##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_619.34100000035c2c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16986345 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74031582426944 33.0 33.0 33.0 33.0 33.0 2 32.7680860714886 33.0 33.0 33.0 33.0 33.0 3 32.79490755662857 33.0 33.0 33.0 33.0 33.0 4 36.71057940951982 37.0 37.0 37.0 37.0 37.0 5 36.721641824653865 37.0 37.0 37.0 37.0 37.0 6 36.672110922037675 37.0 37.0 37.0 37.0 37.0 7 36.63058409563682 37.0 37.0 37.0 37.0 37.0 8 36.621796331111845 37.0 37.0 37.0 37.0 37.0 9 36.60877398875391 37.0 37.0 37.0 37.0 37.0 10-11 36.60001351085239 37.0 37.0 37.0 37.0 37.0 12-13 36.56196606745006 37.0 37.0 37.0 37.0 37.0 14-15 38.64343824407193 40.0 37.0 40.0 37.0 40.0 16-17 38.59668389521113 40.0 37.0 40.0 37.0 40.0 18-19 38.539456133735655 40.0 37.0 40.0 37.0 40.0 20-21 38.48479608179393 40.0 37.0 40.0 37.0 40.0 22-23 38.43555817923161 40.0 37.0 40.0 37.0 40.0 24-25 38.387506140962046 40.0 37.0 40.0 37.0 40.0 26-27 38.28169394298774 40.0 37.0 40.0 37.0 40.0 28-29 38.20778442919887 40.0 37.0 40.0 37.0 40.0 30-31 38.14918898091379 40.0 37.0 40.0 37.0 40.0 32-33 38.057750916986556 40.0 37.0 40.0 37.0 40.0 34-35 37.96795793915642 40.0 37.0 40.0 37.0 40.0 36-37 37.85729887742183 40.0 37.0 40.0 33.0 40.0 38-39 37.73043091377221 40.0 37.0 40.0 33.0 40.0 40-41 37.588901879715735 40.0 37.0 40.0 33.0 40.0 42-43 37.45694594687674 40.0 37.0 40.0 33.0 40.0 44-45 37.308084817540205 40.0 37.0 40.0 33.0 40.0 46-47 37.12691918126001 38.5 37.0 40.0 33.0 40.0 48-49 36.90236328062335 37.0 37.0 40.0 33.0 40.0 50-51 36.68981169874979 37.0 37.0 40.0 33.0 40.0 52-53 36.45187069378374 37.0 37.0 40.0 33.0 40.0 54-55 36.17588033211382 37.0 37.0 40.0 33.0 40.0 56-57 35.94505159879892 37.0 37.0 40.0 33.0 40.0 58-59 35.67142990443206 37.0 35.0 38.5 33.0 40.0 60-61 35.3766858614964 37.0 33.0 37.0 33.0 40.0 62-63 35.11533520012692 37.0 33.0 37.0 27.0 40.0 64-65 34.84259006278278 37.0 33.0 37.0 27.0 40.0 66-67 34.598279088291214 37.0 33.0 37.0 27.0 38.5 68-69 34.34098468505144 37.0 33.0 37.0 27.0 37.0 70-71 34.08718994580647 37.0 33.0 37.0 27.0 37.0 72-73 33.85458107674135 37.0 33.0 37.0 27.0 37.0 74-75 33.58338123946028 37.0 33.0 37.0 27.0 37.0 76-77 30.7742080771349 33.0 30.0 33.0 24.5 37.0 78-79 33.333295067302586 35.0 33.0 37.0 27.0 37.0 80-81 33.83077354192441 37.0 33.0 37.0 27.0 37.0 82-83 33.781835291818226 37.0 33.0 37.0 27.0 37.0 84-85 33.704777484503 37.0 33.0 37.0 27.0 37.0 86-87 33.54979011082137 37.0 33.0 37.0 27.0 37.0 88-89 33.394596071138324 37.0 33.0 37.0 27.0 37.0 90-91 33.30007559012843 37.0 33.0 37.0 27.0 37.0 92-93 33.201060852113855 37.0 33.0 37.0 27.0 37.0 94-95 33.09789636911296 37.0 33.0 37.0 27.0 37.0 96-97 32.96976948248726 35.0 33.0 37.0 27.0 37.0 98-99 32.78384620116923 33.0 33.0 37.0 27.0 37.0 100-101 30.817482572030652 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10219262031827725 2306 2 0.09234819566652419 2306 3 0.08135782225563304 2306 4 0.06116238963531373 2306 5 0.06992544856792193 2306 6 0.04917981303443497 2306 7 0.11083348342494048 2306 8 0.11275918281906172 2306 9 0.08993055867004074 2306 10-11 0.07388806443670859 2306 12-13 0.10640383483615068 2306 14-15 0.2026532932625642 2306 16-17 0.1660035922448273 2306 18-19 0.16266580659745955 2306 20-21 0.16864732730413579 2306 22-23 0.1346614927582337 2306 24-25 0.11925792992726514 2306 26-27 0.09391218534049983 2306 28-29 0.07509074645081881 2306 30-31 0.016864873622289167 2306 32-33 0.019161696448286136 2306 34-35 0.03282681892347483 2306 36-37 0.009381755933560498 2306 38-39 -0.026008941215359016 2306 40-41 -0.044530389844688045 2306 42-43 0.057559469453480006 2306 44-45 0.035199792749374126 2306 46-47 0.04174593239907409 2306 48-49 -0.026427122424756533 2306 50-51 -0.08756452064287146 2306 52-53 -0.045097636564094046 2306 54-55 -0.024180933940201044 2306 56-57 -0.04420641534206737 2306 58-59 -0.10480165494102067 2306 60-61 -0.2023071662363094 2306 62-63 -0.2829777357237546 2306 64-65 -0.22528648473043233 2306 66-67 -0.25685046987508997 2306 68-69 -0.32369460005079986 2306 70-71 -0.31173628043330837 2306 72-73 -0.3028718431229507 2306 74-75 -0.34591994034348517 2306 76-77 -0.31012384656025915 2306 78-79 -0.2936652655165375 2306 80-81 -0.22905854388567093 2306 82-83 -0.30219958536601865 2306 84-85 -0.3326949722586008 2306 86-87 -0.22827296691966126 2306 88-89 -0.3568871159061544 2306 90-91 -0.41092747022725007 2306 92-93 -0.4618379221842446 2306 94-95 -0.522926515020707 2306 96-97 -0.5179168197866701 2306 98-99 -0.414030552640952 2306 100-101 -0.6676927716103833 2307 1 0.1041511770186645 2307 2 0.08103039373884258 2307 3 0.07432517698741492 2307 4 0.04432048780107323 2307 5 0.062136817519522936 2307 6 0.0938350373891268 2307 7 0.11133456498649252 2307 8 0.07621727149574298 2307 9 0.06952375914531927 2307 10-11 0.07671826595131392 2307 12-13 0.07484400126599411 2307 14-15 0.1429654606823263 2307 16-17 0.09735843485643159 2307 18-19 0.17623493142266966 2307 20-21 0.1895697138730057 2307 22-23 0.1347427098055931 2307 24-25 0.09876929266702206 2307 26-27 0.14516442496962867 2307 28-29 0.1357315650302695 2307 30-31 0.08287713385231399 2307 32-33 0.06808401543662512 2307 34-35 0.06501601788665567 2307 36-37 0.030014473539260678 2307 38-39 0.029384604382400425 2307 40-41 -0.025965755574276272 2307 42-43 0.022928892874389817 2307 44-45 -0.028546775651925316 2307 46-47 0.03303653527449768 2307 48-49 -0.04234866163012896 2307 50-51 -0.021899187859915514 2307 52-53 -0.09246477168150591 2307 54-55 -0.02111337506159572 2307 56-57 -0.11469353936455207 2307 58-59 -0.10576400712475476 2307 60-61 -0.1413322560411956 2307 62-63 -0.26253378801192184 2307 64-65 -0.2683060157513637 2307 66-67 -0.32744270743956605 2307 68-69 -0.34645686774149453 2307 70-71 -0.4280523253169122 2307 72-73 -0.47707818901896815 2307 74-75 -0.5308050648661933 2307 76-77 -0.8131008346207551 2307 78-79 -0.3556688794610068 2307 80-81 -0.2932011734097628 2307 82-83 -0.3149046812208738 2307 84-85 -0.4272346815789092 2307 86-87 -0.29259360360470055 2307 88-89 -0.35874826801165227 2307 90-91 -0.3761214995844071 2307 92-93 -0.37366452455158594 2307 94-95 -0.3654672235993175 2307 96-97 -0.4070147771416188 2307 98-99 -0.40050441047168306 2307 100-101 -0.7048361111520904 2201 1 0.07661753538538107 2201 2 0.0853847502626337 2201 3 0.0572598834912057 2201 4 0.03788797839842317 2201 5 0.06988970001109607 2201 6 0.06206126952723423 2201 7 0.07605423139175826 2201 8 0.0719198770887175 2201 9 0.08804464723553451 2201 10-11 0.06469691476670647 2201 12-13 0.09955133384844572 2201 14-15 0.14953731187292618 2201 16-17 0.15457617373735388 2201 18-19 0.16248573513768605 2201 20-21 0.16400248373989257 2201 22-23 0.11625397551364358 2201 24-25 0.08417874732278108 2201 26-27 0.09885610660993649 2201 28-29 0.07775022541317611 2201 30-31 0.0156282357002695 2201 32-33 0.06557368328591906 2201 34-35 0.04323135469174133 2201 36-37 0.014061728604495727 2201 38-39 0.02988407102088786 2201 40-41 0.07912686671015479 2201 42-43 0.040559693030090216 2201 44-45 0.017671589454245407 2201 46-47 -0.0031109114188367926 2201 48-49 -0.015035569182096253 2201 50-51 -0.07993431348222657 2201 52-53 -0.08282139541764622 2201 54-55 -0.06670723579275517 2201 56-57 -0.13433105876323026 2201 58-59 -0.11808742384094728 2201 60-61 -0.1584641468216219 2201 62-63 -0.22382616442834546 2201 64-65 -0.21674533575350097 2201 66-67 -0.2279190156051314 2201 68-69 -0.2789896292453946 2201 70-71 -0.2447293183884014 2201 72-73 -0.2940615295978759 2201 74-75 -0.42749636048767314 2201 76-77 -0.5814594955956878 2201 78-79 -0.2396461168586299 2201 80-81 -0.1814876376735839 2201 82-83 -0.14874721741824004 2201 84-85 -0.18854217570878973 2201 86-87 -0.1729506657597355 2201 88-89 -0.14071003569027596 2201 90-91 -0.26094381652866616 2201 92-93 -0.24490174623196026 2201 94-95 -0.2602997692396727 2201 96-97 -0.43834099912657365 2201 98-99 -0.2529232174823761 2201 100-101 -0.47757586862259416 1101 1 0.06323785278583927 1101 2 0.05714771849348921 1101 3 0.07221566616967579 1101 4 0.04231253956506009 1101 5 0.07140792655385297 1101 6 0.07538026574275847 1101 7 0.0640921271125734 1101 8 0.06949007768809423 1101 9 0.07736820841213188 1101 10-11 0.045743153846878215 1101 12-13 0.061102629782418205 1101 14-15 0.09784626977810973 1101 16-17 0.08234161863224188 1101 18-19 0.03420922812050975 1101 20-21 0.06675560060163122 1101 22-23 0.10130997769770289 1101 24-25 0.0829678001586629 1101 26-27 0.08228029331215936 1101 28-29 0.08245625727116845 1101 30-31 0.05747465789360007 1101 32-33 0.036164009197349856 1101 34-35 8.475169016293194E-4 1101 36-37 0.021776085069525664 1101 38-39 -0.01012095427729065 1101 40-41 -0.015780672717895072 1101 42-43 -0.022447775436589268 1101 44-45 -0.0780648267215227 1101 46-47 -0.027055189808258717 1101 48-49 -0.06478483289492942 1101 50-51 -0.06426118993949359 1101 52-53 -0.0015583423487228742 1101 54-55 -0.06050008080018898 1101 56-57 -0.10906267266410197 1101 58-59 -0.1803266453636425 1101 60-61 -0.21383946187674496 1101 62-63 -0.14351408833583434 1101 64-65 -0.1696488704621686 1101 66-67 -0.24641498553778973 1101 68-69 -0.2202040837397874 1101 70-71 -0.25148387135445205 1101 72-73 -0.2675367057953153 1101 74-75 -0.34589116650915486 1101 76-77 -0.8835635332674165 1101 78-79 -0.31868498323827055 1101 80-81 -0.05272039378466786 1101 82-83 -0.11663737105404692 1101 84-85 -0.07527236553269745 1101 86-87 -0.0523231245780309 1101 88-89 -0.22551836823794957 1101 90-91 -0.26123941612243584 1101 92-93 -0.1399326662194369 1101 94-95 -0.1198223326118395 1101 96-97 -0.233354162607867 1101 98-99 -0.1399242651550452 1101 100-101 -0.28558434874455685 2304 1 0.09714259479829934 2304 2 0.07850476322958855 2304 3 0.06578440910632821 2304 4 0.04683098037259015 2304 5 0.05842858867804068 2304 6 0.0458668740284196 2304 7 0.056698069314386146 2304 8 0.061014679201711886 2304 9 0.08462576196521354 2304 10-11 0.06226712371610432 2304 12-13 0.0712390499436566 2304 14-15 0.1393709078499299 2304 16-17 0.09246619292219549 2304 18-19 0.1182960309688994 2304 20-21 0.13751572208371243 2304 22-23 0.15495312996966248 2304 24-25 0.11867299274684484 2304 26-27 0.09737112601120401 2304 28-29 0.0515283426018982 2304 30-31 0.04145160132937775 2304 32-33 0.050475339898653715 2304 34-35 0.0735167480445611 2304 36-37 0.10003726816101732 2304 38-39 0.02027368800521856 2304 40-41 0.027183119394003086 2304 42-43 0.05891594442005754 2304 44-45 -7.338567943833141E-4 2304 46-47 0.09351995329067364 2304 48-49 0.01774767790335119 2304 50-51 -0.019515033404275073 2304 52-53 -0.07229707728561863 2304 54-55 -0.012604156340472628 2304 56-57 -0.02751203379336431 2304 58-59 -0.06868553856342174 2304 60-61 -0.15839186301505492 2304 62-63 -0.1646264876494854 2304 64-65 -0.21379373605433472 2304 66-67 -0.19017139286953721 2304 68-69 -0.18352073597409202 2304 70-71 -0.19103338987893892 2304 72-73 -0.21169563918473244 2304 74-75 -0.3349072054837663 2304 76-77 -0.8697160143788096 2304 78-79 -0.23853562005362505 2304 80-81 -0.07014698553465593 2304 82-83 -0.07229942232959985 2304 84-85 -0.1452175895739174 2304 86-87 -0.04169324999945445 2304 88-89 -0.18443873481755446 2304 90-91 -0.1855480914800367 2304 92-93 -0.10481262819241266 2304 94-95 -0.13722058160418982 2304 96-97 -0.19069690082168478 2304 98-99 -0.10379757686705204 2304 100-101 -0.1597215905309639 2202 1 0.06864608339630962 2202 2 0.06237797911519749 2202 3 0.04698322366377994 2202 4 0.021554235489702478 2202 5 0.04257722019652732 2202 6 0.028005977681601735 2202 7 0.023929323535064384 2202 8 0.06584818007852533 2202 9 0.060931569967848986 2202 10-11 0.05354258506523735 2202 12-13 0.054389253207105526 2202 14-15 0.045214849923453926 2202 16-17 0.0708351335919275 2202 18-19 0.09121728271990293 2202 20-21 0.10372910621509135 2202 22-23 0.07368651123881875 2202 24-25 0.07924613305154082 2202 26-27 0.0886104367292333 2202 28-29 0.04023735760031144 2202 30-31 0.0295404652951774 2202 32-33 -0.021990339363014755 2202 34-35 -0.06682018826025882 2202 36-37 0.02870722805494097 2202 38-39 0.03581609903118732 2202 40-41 0.041998294012728365 2202 42-43 0.10197923460948743 2202 44-45 0.02538950853868016 2202 46-47 -0.0111745726341681 2202 48-49 0.06931431705744018 2202 50-51 -0.04672167538085148 2202 52-53 -0.056501301269470616 2202 54-55 0.021504523140329468 2202 56-57 -0.028125476461447363 2202 58-59 -0.02028593072944318 2202 60-61 -0.14102632969759554 2202 62-63 -0.16157877825781952 2202 64-65 -0.08816619339783927 2202 66-67 -0.07406500715249109 2202 68-69 -0.17753439022895634 2202 70-71 -0.15537911563205142 2202 72-73 -0.24136580380800154 2202 74-75 -0.33108014563074306 2202 76-77 -0.5864869381404851 2202 78-79 -0.26757296228903726 2202 80-81 -0.025932092766623782 2202 82-83 -0.008581077735321685 2202 84-85 -0.06070648275836987 2202 86-87 0.015615873426007454 2202 88-89 -0.03923606021745485 2202 90-91 -0.06849359024197099 2202 92-93 -0.05328642556899865 2202 94-95 -0.08196893842756481 2202 96-97 -0.10392613110393967 2202 98-99 -0.12693447769207467 2202 100-101 -0.01658496797883302 1102 1 0.07335803758337534 1102 2 0.05699808247830873 1102 3 0.04020068354390105 1102 4 -0.011694730732948244 1102 5 -0.02000256825721891 1102 6 0.04234714428356057 1102 7 0.019208864047719487 1102 8 0.053294731309421195 1102 9 0.0751463873920386 1102 10-11 0.04495323030619858 1102 12-13 0.04072757450974507 1102 14-15 0.03311343022657098 1102 16-17 8.271956682861514E-4 1102 18-19 0.06806246749675182 1102 20-21 0.072040791270922 1102 22-23 0.07440948850901918 1102 24-25 0.05452507658839778 1102 26-27 0.09743784182666815 1102 28-29 0.051726520492614725 1102 30-31 -0.007474601532237557 1102 32-33 -0.005467536492474778 1102 34-35 0.03382279817640921 1102 36-37 0.008257597584382381 1102 38-39 0.0027007034676813646 1102 40-41 0.010991383786119968 1102 42-43 -0.03360599495408678 1102 44-45 -0.026792568151101648 1102 46-47 -0.08681232394261684 1102 48-49 -0.057848605767425454 1102 50-51 -0.1660022353883619 1102 52-53 -0.13933791555655972 1102 54-55 -0.0425741238914199 1102 56-57 -0.1242403444467044 1102 58-59 -0.11307572556733447 1102 60-61 -0.17882038039517312 1102 62-63 -0.1877572723082892 1102 64-65 -0.1339152140044817 1102 66-67 -0.15121397379595436 1102 68-69 -0.19394759597541622 1102 70-71 -0.1803868114131859 1102 72-73 -0.2840548866185273 1102 74-75 -0.3423162357203111 1102 76-77 -0.4258529127598152 1102 78-79 -0.24893905295133578 1102 80-81 -0.1093675639984184 1102 82-83 -0.11442071349022598 1102 84-85 -0.1913905196613186 1102 86-87 -0.21234670631965002 1102 88-89 -0.26886523304793997 1102 90-91 -0.43607704863471497 1102 92-93 -0.29734111368694727 1102 94-95 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1213 46-47 -0.5634852163640645 1213 48-49 -0.9120923632519293 1213 50-51 -0.561896730415576 1213 52-53 -0.22305750234203714 1213 54-55 -0.7805904482962589 1213 56-57 -0.2739427266358163 1213 58-59 -0.10755043265116626 1213 60-61 -0.08931721609062038 1213 62-63 0.03334723363393266 1213 64-65 0.04194899632555149 1213 66-67 -0.3287190911597264 1213 68-69 0.014414241440888986 1213 70-71 0.04360022647577466 1213 72-73 0.14308379012047823 1213 74-75 0.16947398898022215 1213 76-77 0.7129213611701388 1213 78-79 0.07257317183488254 1213 80-81 -0.09756741961412274 1213 82-83 -0.004572803562659544 1213 84-85 -0.0037685836328620326 1213 86-87 -1.2970354281348975 1213 88-89 -0.5644189244426983 1213 90-91 -0.258297977889967 1213 92-93 -0.13077045651640162 1213 94-95 0.16630094275843277 1213 96-97 0.22895532085313874 1213 98-99 0.21119672205013273 1213 100-101 0.6270512633514613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 29.0 8 405.0 9 1123.0 10 804.0 11 332.0 12 346.0 13 837.0 14 2380.0 15 5663.0 16 10557.0 17 15729.0 18 20955.0 19 26248.0 20 31854.0 21 39108.0 22 47196.0 23 56786.0 24 69337.0 25 84929.0 26 104241.0 27 129077.0 28 162299.0 29 205651.0 30 263807.0 31 346183.0 32 466894.0 33 661763.0 34 1036234.0 35 1944064.0 36 4317626.0 37 5922047.0 38 1011790.0 39 50.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.962471113766373 22.997118437700166 31.742163826920404 25.298246621613057 2 26.165128519407794 22.560892293191973 31.732459219449506 19.541519967950727 3 21.320590156387382 20.99159059821286 38.23469380846792 19.453125436931842 4 21.00958828176407 20.603693623712495 37.82026732367121 20.566450770852228 5 27.788974025901393 20.044435692316387 33.10509117764887 19.06149910413335 6 28.827849663950662 20.36823107030971 32.265970107165494 18.537949158574136 7 28.401377694848424 25.88980148466312 26.91225216490069 18.796568655587766 8 26.813926127133293 22.191742838144403 31.264989613716192 19.729341421006108 9 22.475417754672943 21.13144410996009 35.88301662305811 20.510121512308856 10-11 27.31780371659601 21.87133380932488 29.58186116620127 21.22900130787783 12-13 26.426042652109555 25.977099414826665 25.635542500355214 21.96131543270857 14-15 26.432263680032403 23.753673906894036 26.714646382138124 23.099416030935437 16-17 25.810406036705675 23.44522723612689 26.552884475707444 24.191482251459995 18-19 25.439136553508128 24.17800003473378 27.173453147219135 23.209410264538956 20-21 24.263212273564854 25.09863064505684 27.50967850183385 23.12847857954445 22-23 24.38355553094755 24.957799187329517 27.052952861809253 23.60569241991368 24-25 24.563677471521977 25.119259028354833 26.786642447212746 23.53042105291044 26-27 24.401445806993113 25.15759094885308 26.86008243268677 23.580880811467033 28-29 24.390392658950034 25.51269714071492 26.374928000987147 23.7219821993479 30-31 24.553677738528297 25.089612381523768 26.428539427828913 23.928170452119023 32-33 24.423141058303006 25.30003658821247 26.38868455809652 23.888137795388 34-35 24.29365175380578 25.476319361228093 26.444402842400766 23.785626042565365 36-37 24.701959915785448 25.217050514955197 26.458692376101027 23.622297193158325 38-39 24.70112894495854 25.041181544651085 26.395731219651065 23.861958290739306 40-41 24.568920752609067 25.295792407970332 26.18093241533666 23.95435442408394 42-43 24.552843140080167 25.261818221101713 26.21282692856049 23.97251171025763 44-45 24.51217571191142 25.085751757342535 26.47740508713644 23.9246674436096 46-47 24.222693651982087 25.385847809719653 26.401310927632508 23.99014761066575 48-49 24.172588257767675 25.13180932931477 26.40083925109745 24.294763161820104 50-51 24.358414280257303 25.277489415236875 26.660718011736616 23.7033782927692 52-53 24.458658642928388 25.467315506268907 26.361160248899722 23.712865601902987 54-55 24.286117721479748 25.282675135500988 26.24412400728065 24.187083135738614 56-57 24.436748298653622 25.391959334611876 26.289897746846 23.88139461988851 58-59 24.296166681937223 25.518213613344315 26.27159772971962 23.91402197499884 60-61 24.545078159742133 25.490174547857166 25.95399420095168 24.01075309144903 62-63 24.13877708295955 25.79295208325024 26.3378470924661 23.730423741324113 64-65 24.436199982153713 25.53651981104133 25.981081346594458 24.046198860210495 66-67 24.6276416109576 25.588875165066185 26.000306209727164 23.783177014249056 68-69 24.373815373732693 25.43261935891763 26.44877885959527 23.74478640775441 70-71 24.293037844702194 25.73998453597564 26.046271017507365 23.920706601814796 72-73 24.115895770995767 25.423736497697263 26.26427361055667 24.1960941207503 74-75 24.22451232145305 25.48426612793433 26.226804383536344 24.064417167076275 76-77 24.26174156907314 25.289871132197582 26.196781670825974 24.2516056279033 78-79 24.585678978968406 25.29775064118272 26.006510561248742 24.11005981860013 80-81 24.24664866635162 25.37398300800559 26.27911670076043 24.10025162488235 82-83 24.397165204062613 25.776929048386798 26.101127086832065 23.724778660718524 84-85 24.247805644100577 25.969081980654778 25.913238708981513 23.869873666263132 86-87 24.021912799113323 25.78890763609506 26.127187546956332 24.061992017835284 88-89 23.862989637057513 26.7502158228733 25.72836521242938 23.658429327639805 90-91 23.907250421025385 25.942052399556214 25.832606994379187 24.318090185039214 92-93 23.811058106505843 26.419142589002586 25.667372397013967 24.102426907477607 94-95 23.66590435882817 26.448303433223614 25.372090276123227 24.513701931824993 96-97 23.607028505299738 26.105123987107802 25.55316405565044 24.73468345194202 98-99 23.91515922357117 26.14308786365498 25.27984816222367 24.661904750550185 100-101 24.12967843768014 26.61436207572838 24.75256798904345 24.503391497548026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 62531.0 1 34387.5 2 10725.0 3 13126.5 4 12318.0 5 9091.0 6 4092.0 7 4023.5 8 4887.0 9 5367.5 10 4992.0 11 3923.0 12 2929.5 13 2388.0 14 2180.0 15 2142.0 16 2231.0 17 2383.0 18 2543.5 19 2774.0 20 3027.0 21 3442.5 22 3890.5 23 4378.0 24 5246.5 25 6194.0 26 6938.5 27 7991.0 28 9490.5 29 11387.5 30 14137.5 31 19392.5 32 33617.5 33 52829.5 34 71365.0 35 102369.0 36 141073.0 37 202888.5 38 284583.0 39 331968.5 40 379722.5 41 450757.5 42 519196.0 43 595558.0 44 717699.5 45 851683.0 46 965248.0 47 1030157.0 48 1068154.0 49 1111611.5 50 1082863.5 51 1007078.5 52 922220.5 53 821130.5 54 711353.0 55 634214.5 56 588850.0 57 505734.5 58 405373.0 59 327023.5 60 251808.0 61 180439.0 62 122657.5 63 78966.0 64 48293.5 65 30951.5 66 21194.5 67 14822.0 68 11699.5 69 9427.0 70 7334.0 71 5810.0 72 5475.0 73 5121.0 74 4065.0 75 3155.5 76 2506.5 77 2181.5 78 2005.0 79 1823.0 80 1435.5 81 1044.5 82 817.0 83 693.5 84 529.0 85 345.5 86 289.0 87 281.5 88 246.5 89 238.5 90 235.0 91 194.5 92 153.5 93 132.0 94 111.5 95 74.0 96 62.0 97 65.5 98 38.5 99 9.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15978717022408293 2 0.0 3 0.0 4 0.08218954695668786 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.8846700099403373E-4 12-13 6.181435735586437E-5 14-15 0.0 16-17 3.561684400028376E-4 18-19 0.0 20-21 0.0014423350049701687 22-23 8.830622479409196E-6 24-25 0.0 26-27 7.182239616586146E-4 28-29 5.887081652939464E-6 30-31 7.770947781880091E-4 32-33 0.0 34-35 0.0 36-37 0.001218625902158469 38-39 0.005207123722024956 40-41 0.005610388815251309 42-43 0.0016218909953848223 44-45 0.0031878547150667198 46-47 0.019171281402797363 48-49 0.00988146655445889 50-51 0.009142637807014988 52-53 0.012218637970675858 54-55 0.011035334558435026 56-57 0.015680241982604263 58-59 0.022211959076540597 60-61 0.010796907751490977 62-63 0.004047368636395881 64-65 0.014243794059287034 66-67 0.026609609071286375 68-69 0.030913065759585127 70-71 0.014181979701931169 72-73 0.012981015044731517 74-75 0.025061306596563297 76-77 0.042163278798352445 78-79 0.007812157353450669 80-81 0.0020369302519170547 82-83 0.002537332192416909 84-85 0.012975127963078578 86-87 0.014605849580942808 88-89 0.018188138766756474 90-91 0.009542959359414871 92-93 0.019012330198168 94-95 0.026956946888809806 96-97 0.017937937796506546 98-99 0.008097680813618233 100-101 0.32008357301114515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6986345E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.678268595496444 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48797023397447 12.461052705475586 2 11.487356646708305 4.061531525055416 3 4.874699535944278 2.5852874315629446 4 2.5488052508325665 1.802338552873192 5 1.5201821958020385 1.343709458574 6 1.2037924711579 1.2768579983019455 7 0.8872370504054641 1.0979370419460666 8 0.6339065446577037 0.8965096128721557 9 0.619981870862323 0.9864185433698283 >10 4.355522904388025 15.942215764053067 >50 0.6539060948283407 8.1499912239498 >100 0.5766546495792988 19.679275680637236 >500 0.09068709451453022 11.402581081295727 >1k 0.05878426670972192 17.191906265372708 >5k 3.4746979791685443E-4 0.36843678937338564 >10k+ 1.65719837244988E-4 0.7539503252869646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39800 0.23430584978699065 No Hit CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT 32233 0.18975830291919774 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29594 0.1742222944370905 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.24191431411525E-5 0.0 0.0 2 8.24191431411525E-5 0.0 0.0 3 9.419330644703143E-5 0.0 0.0 4 1.0008038809997089E-4 0.0 0.0 5 1.0596746975291035E-4 0.0 0.0 6 1.2951579636466822E-4 0.0 0.0 7 1.4128995967054714E-4 0.0 0.0 8 1.5895120462936554E-4 0.0 0.0 9 2.0016077619994179E-4 0.0 0.0 10-11 2.53144511076397E-4 0.0 0.0 12-13 3.885473890940046E-4 0.0 0.0 14-15 5.945952469468859E-4 0.0 0.0 16-17 8.712880846350407E-4 0.0 5.8870816529394644E-6 18-19 0.0012362871471172875 0.0 5.8870816529394644E-6 20-21 0.001701366597699505 0.0 5.8870816529394644E-6 22-23 0.0023283407937375582 0.0 5.8870816529394644E-6 24-25 0.002975919775560899 0.0 5.8870816529394644E-6 26-27 0.0036382164615165885 0.0 5.8870816529394644E-6 28-29 0.0043034566882987485 0.0 5.8870816529394644E-6 30-31 0.005180631854586728 0.0 5.8870816529394644E-6 32-33 0.006116677837404104 0.0 5.8870816529394644E-6 34-35 0.0071704654532802675 0.0 5.8870816529394644E-6 36-37 0.008450905712794601 0.0 5.8870816529394644E-6 38-39 0.009931506748508876 0.0 5.8870816529394644E-6 40-41 0.011544567121414289 0.0 5.8870816529394644E-6 42-43 0.013372505974651993 0.0 5.8870816529394644E-6 44-45 0.015353508950866124 0.0 8.830622479409196E-6 46-47 0.017605317683115466 0.0 1.1774163305878929E-5 48-49 0.020384020223302893 0.0 1.1774163305878929E-5 50-51 0.02351006058101375 0.0 1.1774163305878929E-5 52-53 0.027268962216415598 0.0 1.1774163305878929E-5 54-55 0.03176080551760841 0.0 1.1774163305878929E-5 56-57 0.036976759862112775 0.0 1.1774163305878929E-5 58-59 0.04360267026249614 0.0 1.7661244958818392E-5 60-61 0.051417771156773276 0.0 1.7661244958818392E-5 62-63 0.06094895635288227 0.0 1.7661244958818392E-5 64-65 0.07315287661942578 0.0 1.7661244958818392E-5 66-67 0.08838570039640664 0.0 1.7661244958818392E-5 68-69 0.10737742580878935 0.0 1.7661244958818392E-5 70-71 0.13031938301029444 0.0 1.7661244958818392E-5 72-73 0.1588275759146538 0.0 1.7661244958818392E-5 74-75 0.19437377493510227 0.0 1.7661244958818392E-5 76-77 0.23850922608718944 0.0 1.7661244958818392E-5 78-79 0.29397436588035863 0.0 1.7661244958818392E-5 80-81 0.36295330160785033 0.0 1.7661244958818392E-5 82-83 0.44714739986736407 0.0 1.7661244958818392E-5 84-85 0.5488496789627197 0.0 1.7661244958818392E-5 86-87 0.6734114961164395 0.0 1.7661244958818392E-5 88-89 0.820832851328523 0.0 1.7661244958818392E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 175730 0.0 60.203724 1 TTTGTGT 192040 0.0 55.176437 2 ACTTTGT 59690 0.0 52.915928 1 TTGTGTT 209205 0.0 52.064533 3 TGTGTTT 224585 0.0 48.757217 4 TAACTTT 31970 0.0 48.33402 1 AACTTTG 32500 0.0 47.817047 2 GTGTTTG 263500 0.0 41.554264 5 TTTGAGG 86945 0.0 32.70612 8 TTGAGGG 50875 0.0 32.189045 9 GTTTGAG 208920 0.0 31.951683 7 TGTTTGA 351535 0.0 31.142506 6 TTGAGTC 23850 0.0 28.8535 4 TTTGAGT 77820 0.0 27.298239 3 TTGAGGC 14555 0.0 26.619339 9 GTTTGAC 65320 0.0 26.393646 7 GTTTGAA 19425 0.0 26.30061 2 TTTGAGA 25865 0.0 26.25082 8 TGAGTCT 3855 0.0 24.63336 5 TTTGATG 25420 0.0 24.54365 8 >>END_MODULE