FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_619.34100000035c2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_619.34100000035c2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16986345
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398000.23430584978699065No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT322330.18975830291919774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295940.1742222944370905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1757300.060.2037241
TTTGTGT1920400.055.1764372
ACTTTGT596900.052.9159281
TTGTGTT2092050.052.0645333
TGTGTTT2245850.048.7572174
TAACTTT319700.048.334021
AACTTTG325000.047.8170472
GTGTTTG2635000.041.5542645
TTTGAGG869450.032.706128
TTGAGGG508750.032.1890459
GTTTGAG2089200.031.9516837
TGTTTGA3515350.031.1425066
TTGAGTC238500.028.85354
TTTGAGT778200.027.2982393
TTGAGGC145550.026.6193399
GTTTGAC653200.026.3936467
GTTTGAA194250.026.300612
TTTGAGA258650.026.250828
TGAGTCT38550.024.633365
TTTGATG254200.024.543658
Produced by FastQC (version 0.11.2)