##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_30515.34100000035be7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17987162 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74510575931878 33.0 33.0 33.0 33.0 33.0 2 32.77318195054895 33.0 33.0 33.0 33.0 33.0 3 32.79987854671015 33.0 33.0 33.0 33.0 33.0 4 36.72081788110876 37.0 37.0 37.0 37.0 37.0 5 36.73113618479669 37.0 37.0 37.0 37.0 37.0 6 36.67893095086373 37.0 37.0 37.0 37.0 37.0 7 36.64312141070393 37.0 37.0 37.0 37.0 37.0 8 36.635589927971964 37.0 37.0 37.0 37.0 37.0 9 36.625871607761134 37.0 37.0 37.0 37.0 37.0 10-11 36.619859903413335 37.0 37.0 37.0 37.0 37.0 12-13 36.58823131742517 37.0 37.0 37.0 37.0 37.0 14-15 38.69484352228551 40.0 37.0 40.0 37.0 40.0 16-17 38.65919242846648 40.0 37.0 40.0 37.0 40.0 18-19 38.61949747825699 40.0 37.0 40.0 37.0 40.0 20-21 38.57054881698403 40.0 37.0 40.0 37.0 40.0 22-23 38.530934563217926 40.0 37.0 40.0 37.0 40.0 24-25 38.496147307729814 40.0 37.0 40.0 37.0 40.0 26-27 38.40601413385836 40.0 37.0 40.0 37.0 40.0 28-29 38.34348209017075 40.0 37.0 40.0 37.0 40.0 30-31 38.288766121081245 40.0 37.0 40.0 37.0 40.0 32-33 38.2034668948887 40.0 37.0 40.0 37.0 40.0 34-35 38.11879967501265 40.0 37.0 40.0 37.0 40.0 36-37 38.02306489484 40.0 37.0 40.0 37.0 40.0 38-39 37.89589677904719 40.0 37.0 40.0 33.0 40.0 40-41 37.76269836230974 40.0 37.0 40.0 33.0 40.0 42-43 37.628006574911595 40.0 37.0 40.0 33.0 40.0 44-45 37.46151040947983 40.0 37.0 40.0 33.0 40.0 46-47 37.255637687590735 38.5 37.0 40.0 33.0 40.0 48-49 37.01077043171124 37.0 37.0 40.0 33.0 40.0 50-51 36.79456848167599 37.0 37.0 40.0 33.0 40.0 52-53 36.546254322944336 37.0 37.0 40.0 33.0 40.0 54-55 36.24916901843659 37.0 37.0 40.0 33.0 40.0 56-57 35.98863230897682 37.0 37.0 40.0 33.0 40.0 58-59 35.69760104456723 37.0 35.0 37.0 33.0 40.0 60-61 35.40415377923432 37.0 33.0 37.0 33.0 40.0 62-63 35.1402320443881 37.0 33.0 37.0 33.0 40.0 64-65 34.85200636431695 37.0 33.0 37.0 27.0 40.0 66-67 34.611282980605836 37.0 33.0 37.0 27.0 37.0 68-69 34.36313624684094 37.0 33.0 37.0 27.0 37.0 70-71 34.131655899913504 37.0 33.0 37.0 27.0 37.0 72-73 33.93847225593454 37.0 33.0 37.0 27.0 37.0 74-75 33.693589572385015 37.0 33.0 37.0 27.0 37.0 76-77 30.82791209641632 33.0 30.0 33.0 24.5 37.0 78-79 33.47184842166874 35.0 33.0 37.0 27.0 37.0 80-81 34.00115593555003 37.0 33.0 37.0 27.0 37.0 82-83 33.97908196968482 37.0 33.0 37.0 27.0 37.0 84-85 33.907910931140776 37.0 33.0 37.0 27.0 37.0 86-87 33.756448932855555 37.0 33.0 37.0 27.0 37.0 88-89 33.598424392908676 37.0 33.0 37.0 27.0 37.0 90-91 33.51752733421759 37.0 33.0 37.0 27.0 37.0 92-93 33.42168055749984 37.0 33.0 37.0 27.0 37.0 94-95 33.323772783054935 37.0 33.0 37.0 27.0 37.0 96-97 33.21173165060725 37.0 33.0 37.0 27.0 37.0 98-99 33.03731578111099 33.0 33.0 37.0 27.0 37.0 100-101 31.007858243562826 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10076704522770541 2306 2 0.08789380533667668 2306 3 0.08007746935511761 2306 4 0.03390969962055124 2306 5 0.03862404913943607 2306 6 0.05818027216764676 2306 7 0.11024047660357184 2306 8 0.12091611047262063 2306 9 0.09867842214508471 2306 10-11 0.08662564932532746 2306 12-13 0.09761908449959122 2306 14-15 0.1592387270537472 2306 16-17 0.15667256710266741 2306 18-19 0.19735437698441416 2306 20-21 0.17818175624312005 2306 22-23 0.15958346690003822 2306 24-25 0.13752510523703876 2306 26-27 0.13573759007027064 2306 28-29 0.1202891704448561 2306 30-31 0.072246121963353 2306 32-33 0.11604533003779238 2306 34-35 0.050368169942267116 2306 36-37 0.040254705485772035 2306 38-39 0.06080529513156563 2306 40-41 0.04893350843138933 2306 42-43 0.05389943247060103 2306 44-45 -0.032617446616505674 2306 46-47 -0.01491840039638248 2306 48-49 -0.03647129831953322 2306 50-51 -0.043849851539512485 2306 52-53 -0.04612535164623921 2306 54-55 0.008976076745355499 2306 56-57 -0.03678676586490326 2306 58-59 -0.06324165141642624 2306 60-61 -0.20296513338878697 2306 62-63 -0.1941370382559242 2306 64-65 -0.2185145160537303 2306 66-67 -0.25378916862614886 2306 68-69 -0.27567149469354746 2306 70-71 -0.339192369739024 2306 72-73 -0.3245995960982313 2306 74-75 -0.3567933706644908 2306 76-77 -0.29488715413068434 2306 78-79 -0.2606898804581519 2306 80-81 -0.19987114854900767 2306 82-83 -0.26083100931760583 2306 84-85 -0.33769053786914327 2306 86-87 -0.2332745061773096 2306 88-89 -0.4225760511716885 2306 90-91 -0.4593197012772592 2306 92-93 -0.39543856524149845 2306 94-95 -0.3952959406936216 2306 96-97 -0.41985994446917374 2306 98-99 -0.3867254810738032 2306 100-101 -0.7473760059143899 2307 1 0.11763278990737547 2307 2 0.10788453132720832 2307 3 0.09334452274136851 2307 4 0.06717092042182315 2307 5 0.07019272386680342 2307 6 0.10205557529891252 2307 7 0.10105846228535853 2307 8 0.09367934455967486 2307 9 0.06943542116837165 2307 10-11 0.09000354648276243 2307 12-13 0.09292638219761074 2307 14-15 0.16349291266005395 2307 16-17 0.14461623937749124 2307 18-19 0.1645922991320461 2307 20-21 0.17646600416124159 2307 22-23 0.13461739326006494 2307 24-25 0.08482322765669181 2307 26-27 0.13224881451756687 2307 28-29 0.10365287067705964 2307 30-31 0.058758030498680114 2307 32-33 0.07787045279330584 2307 34-35 0.08085080769341602 2307 36-37 0.049883223647206876 2307 38-39 0.07254532394509994 2307 40-41 -0.029080869903076234 2307 42-43 0.01198084408837019 2307 44-45 -0.03424162847816348 2307 46-47 0.043775445759557385 2307 48-49 0.00596375030161056 2307 50-51 0.04008545303757671 2307 52-53 -0.07228184601819976 2307 54-55 -0.03837735240855977 2307 56-57 -0.11130196830512773 2307 58-59 -0.13302421581696677 2307 60-61 -0.1571827433677413 2307 62-63 -0.179798256560062 2307 64-65 -0.20070311933586993 2307 66-67 -0.264834735289746 2307 68-69 -0.30755861397678785 2307 70-71 -0.37039805287385263 2307 72-73 -0.4237011298718798 2307 74-75 -0.5017642979720591 2307 76-77 -0.8336324501073591 2307 78-79 -0.37227174801220286 2307 80-81 -0.23003439220490662 2307 82-83 -0.26346164521415716 2307 84-85 -0.4002961688789881 2307 86-87 -0.2710207870518957 2307 88-89 -0.3274137523337046 2307 90-91 -0.34213252177958964 2307 92-93 -0.2899202515646735 2307 94-95 -0.3856151532747134 2307 96-97 -0.45936373617402637 2307 98-99 -0.37664697829909954 2307 100-101 -0.6475612552765462 2201 1 0.07952871660506133 2201 2 0.07087910360967697 2201 3 0.06895632399379537 2201 4 0.01918312513603837 2201 5 0.06096679383337289 2201 6 0.053941151060143966 2201 7 0.08909889306958974 2201 8 0.07506755197342585 2201 9 0.08916927269645214 2201 10-11 0.07342102901417746 2201 12-13 0.08800826505258641 2201 14-15 0.14424934378093468 2201 16-17 0.1441774935324176 2201 18-19 0.11800354250811296 2201 20-21 0.14433479232025093 2201 22-23 0.13033646999041792 2201 24-25 0.05136155498433226 2201 26-27 0.0649837428544302 2201 28-29 0.12790011680456104 2201 30-31 0.060559838144982336 2201 32-33 0.10456190993890857 2201 34-35 0.05512435590247833 2201 36-37 0.025603342276845353 2201 38-39 0.010524900564817585 2201 40-41 0.09691131516682816 2201 42-43 0.07581994722460195 2201 44-45 0.03946329389373204 2201 46-47 0.017680681592338487 2201 48-49 0.03412908121062941 2201 50-51 -0.030176660522691634 2201 52-53 0.03511575640899878 2201 54-55 0.022919134574379996 2201 56-57 -0.10800833763423867 2201 58-59 -0.09230432901971852 2201 60-61 -0.14770807701664523 2201 62-63 -0.19052810798805808 2201 64-65 -0.2050276305208314 2201 66-67 -0.15056122673943406 2201 68-69 -0.23611289205784658 2201 70-71 -0.2412270688288629 2201 72-73 -0.28308190288378654 2201 74-75 -0.4365786966872278 2201 76-77 -0.6138684138561175 2201 78-79 -0.26363117638552325 2201 80-81 -0.20496021685864463 2201 82-83 -0.12447425870737305 2201 84-85 -0.15501346091615886 2201 86-87 -0.13572897275223283 2201 88-89 -0.06653351831372589 2201 90-91 -0.1550434214219223 2201 92-93 -0.10753994598756123 2201 94-95 -0.17404518731417085 2201 96-97 -0.2656486582217923 2201 98-99 -0.1450254182497943 2201 100-101 -0.4611299370766382 1101 1 0.07598305341463885 1101 2 0.06753515743685767 1101 3 0.07327125553977254 1101 4 0.0711154975539614 1101 5 0.0586660175216025 1101 6 0.08648939049542292 1101 7 0.07026172001391018 1101 8 0.062158725866034104 1101 9 0.09396127983465874 1101 10-11 0.05477583169000155 1101 12-13 0.09487201831165493 1101 14-15 0.11229963902163576 1101 16-17 0.08740662064133886 1101 18-19 0.07165173571306127 1101 20-21 0.11892958689220023 1101 22-23 0.12844337978980747 1101 24-25 0.12689784256718895 1101 26-27 0.1513727624620742 1101 28-29 0.12981901270758556 1101 30-31 0.081968041763119 1101 32-33 0.06989445508642689 1101 34-35 0.06377958482691781 1101 36-37 0.06713429837957818 1101 38-39 0.04992430198249309 1101 40-41 0.04566367360275336 1101 42-43 -0.020490925091721124 1101 44-45 -0.02834326483814209 1101 46-47 0.0457726812674224 1101 48-49 -0.007169114970771773 1101 50-51 0.004006057669663221 1101 52-53 0.04414582782334975 1101 54-55 -0.021591175096979498 1101 56-57 -0.028450873723365078 1101 58-59 -0.08383581571555254 1101 60-61 -0.15817836046736033 1101 62-63 -0.09051472219633183 1101 64-65 -0.08995872507996694 1101 66-67 -0.1230151285543073 1101 68-69 -0.10855099796034295 1101 70-71 -0.21862581425384775 1101 72-73 -0.23153318868222783 1101 74-75 -0.2645341955598468 1101 76-77 -0.8214782085057735 1101 78-79 -0.26561083390425466 1101 80-81 -0.040849053956918624 1101 82-83 -0.024415983273136987 1101 84-85 -0.0377336271781914 1101 86-87 0.06498046321282658 1101 88-89 -0.08769140845122791 1101 90-91 -0.1359509817669462 1101 92-93 -0.02664289945020215 1101 94-95 -0.013935397429769125 1101 96-97 -0.15128063052670626 1101 98-99 -0.10068043552347916 1101 100-101 -0.2709962800332484 2304 1 0.08350818153525807 2304 2 0.07091773251994482 2304 3 0.048774559018760044 2304 4 0.03782797977009977 2304 5 0.033703421923164 2304 6 0.04636345467631031 2304 7 0.05301178472967649 2304 8 0.07075853875409166 2304 9 0.06996766110027863 2304 10-11 0.06224112539306503 2304 12-13 0.06941057990106714 2304 14-15 0.10875047962796458 2304 16-17 0.09772357841137591 2304 18-19 0.11994156524389865 2304 20-21 0.10466822706061407 2304 22-23 0.11371234420878551 2304 24-25 0.0819966368476841 2304 26-27 0.12267565221323906 2304 28-29 0.05029956899065979 2304 30-31 0.07019264654725532 2304 32-33 0.09339648602367845 2304 34-35 0.10304231653947227 2304 36-37 0.12227447261961544 2304 38-39 0.07478590889619596 2304 40-41 0.09731871028147765 2304 42-43 0.08349404409098327 2304 44-45 0.038658884877278865 2304 46-47 0.08375236487336224 2304 48-49 0.07291351333767437 2304 50-51 -0.015834879974363503 2304 52-53 -0.02393871781521284 2304 54-55 0.03485527151254075 2304 56-57 -0.01924793691058113 2304 58-59 0.003743634048895217 2304 60-61 -0.017308484254698442 2304 62-63 -0.011540414167384938 2304 64-65 -0.0684395096322703 2304 66-67 -0.08342175104615279 2304 68-69 -0.10169395381982582 2304 70-71 -0.08440476152159704 2304 72-73 -0.11983848410644526 2304 74-75 -0.23591770547626822 2304 76-77 -0.7637087787800425 2304 78-79 -0.17910277647940376 2304 80-81 -0.05069350401398509 2304 82-83 0.0027671138666534034 2304 84-85 -0.012854334544051937 2304 86-87 0.07131724970847131 2304 88-89 -0.10648751800606959 2304 90-91 -0.0747636617241696 2304 92-93 0.012547887417483139 2304 94-95 -0.016471407813483552 2304 96-97 -0.08073248197540295 2304 98-99 -0.01529545053021053 2304 100-101 -0.08378575859084947 2202 1 0.08596640152867963 2202 2 0.07361215236109331 2202 3 0.0654744698726546 2202 4 0.009874884877774548 2202 5 0.025402342219230434 2202 6 0.007004113026887637 2202 7 0.020067841115100293 2202 8 0.05449461280302614 2202 9 0.06235795794851384 2202 10-11 0.060587127612414804 2202 12-13 0.03975244786426657 2202 14-15 0.06337552539256563 2202 16-17 0.059484344380415166 2202 18-19 0.05401488834434076 2202 20-21 0.07120772291877131 2202 22-23 0.07406718868464424 2202 24-25 0.06470410990306874 2202 26-27 0.059775301756751276 2202 28-29 0.04432549420653942 2202 30-31 0.010799272776189639 2202 32-33 -0.020403167892240504 2202 34-35 -0.03522153787814375 2202 36-37 0.0056133042667099176 2202 38-39 0.040212608396061 2202 40-41 0.035488923145180706 2202 42-43 0.04437389036953476 2202 44-45 -0.0098021799654191 2202 46-47 0.058420095271983996 2202 48-49 0.01574798411031253 2202 50-51 -0.03645230553958356 2202 52-53 -0.025655528857221555 2202 54-55 4.5087445299429874E-4 2202 56-57 -0.05972711341618009 2202 58-59 -0.03987165264971537 2202 60-61 -0.10124076258446024 2202 62-63 -0.11645021146931356 2202 64-65 0.015197404040549145 2202 66-67 -0.05220033007514502 2202 68-69 -0.1626146713758203 2202 70-71 -0.0960336746044348 2202 72-73 -0.1772443845989855 2202 74-75 -0.2594875655619191 2202 76-77 -0.5803570719320064 2202 78-79 -0.1736056850279084 2202 80-81 0.03915665391333789 2202 82-83 0.05892741352290187 2202 84-85 0.05945666189477805 2202 86-87 0.11531061161745271 2202 88-89 0.03914312935280151 2202 90-91 -0.07235428053856907 2202 92-93 -0.029708403157961527 2202 94-95 -0.040994771073556535 2202 96-97 -0.10734895750747597 2202 98-99 -0.08250287358601582 2202 100-101 -0.01688484448029115 1102 1 0.06465669812105546 1102 2 0.05323636162826517 1102 3 0.04655774483181574 1102 4 0.0028160129908414433 1102 5 0.02782452235603472 1102 6 0.04574059614697035 1102 7 0.07660718734941696 1102 8 0.06389211244504622 1102 9 0.08007504301809831 1102 10-11 0.045849630570707234 1102 12-13 0.046398283066686474 1102 14-15 0.07285436133025058 1102 16-17 0.06511012877336242 1102 18-19 0.07553787997853334 1102 20-21 0.08520830526863676 1102 22-23 0.12244669922421991 1102 24-25 0.08627290192587367 1102 26-27 0.07748574622967652 1102 28-29 0.08338406446060276 1102 30-31 0.058132110743102317 1102 32-33 0.043514501810342665 1102 34-35 0.06469830378031105 1102 36-37 0.048728162416900034 1102 38-39 0.06113880250251924 1102 40-41 0.0520814010481061 1102 42-43 0.03963708784191766 1102 44-45 0.021515312496248384 1102 46-47 -0.028681228549253035 1102 48-49 -0.024305304235774372 1102 50-51 -0.0540287218177653 1102 52-53 -0.04539527416324063 1102 54-55 0.040621012017346914 1102 56-57 -0.012016964293700028 1102 58-59 -0.021456628804479294 1102 60-61 -0.10567971893320305 1102 62-63 -0.11341459304799173 1102 64-65 -0.09204018168290418 1102 66-67 -0.07136225536473972 1102 68-69 -0.12126988370597758 1102 70-71 -0.06917610032877519 1102 72-73 -0.12573978858591772 1102 74-75 -0.19455760613946893 1102 76-77 -0.3217634713909199 1102 78-79 -0.1441285687117002 1102 80-81 0.012835527606853248 1102 82-83 -0.027197733012670255 1102 84-85 -0.10225317509858911 1102 86-87 -0.08746082329768967 1102 88-89 -0.15300944370888914 1102 90-91 -0.2011736655798657 1102 92-93 -0.18325976437110114 1102 94-95 -0.21079384887530495 1102 96-97 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54-55 -0.8318904887134693 1213 56-57 -0.35046529413043714 1213 58-59 -0.19162237392423975 1213 60-61 -0.19528203328933103 1213 62-63 -0.08086595443695899 1213 64-65 -0.05113948918116762 1213 66-67 -0.3931831307371496 1213 68-69 -0.0763249998607094 1213 70-71 -0.03408387381875855 1213 72-73 0.015974252561321123 1213 74-75 0.09832824463764211 1213 76-77 0.6552642929797088 1213 78-79 -0.04556429683954377 1213 80-81 -0.20396729169237204 1213 82-83 -0.13072983995193255 1213 84-85 -0.10412890648569118 1213 86-87 -1.3646737790854289 1213 88-89 -0.6539413426488423 1213 90-91 -0.3400868297419848 1213 92-93 -0.25226363090665416 1213 94-95 0.036496823402693224 1213 96-97 0.11223636140945104 1213 98-99 0.08973533406729217 1213 100-101 0.5154998867641716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 29.0 8 333.0 9 872.0 10 679.0 11 280.0 12 236.0 13 475.0 14 1177.0 15 2596.0 16 4871.0 17 7627.0 18 10832.0 19 14931.0 20 19877.0 21 26440.0 22 34719.0 23 44941.0 24 58281.0 25 75425.0 26 97152.0 27 125865.0 28 160673.0 29 207565.0 30 270125.0 31 356092.0 32 485952.0 33 707838.0 34 1136958.0 35 2173949.0 36 4818996.0 37 6256682.0 38 884643.0 39 50.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.214136942888146 22.01046316800368 30.442546600712184 27.33285328839599 2 27.272812687182114 22.433611261187284 30.49784062655354 19.795735425077062 3 21.337818606403832 20.570949436047776 37.383507192518756 20.707724765029635 4 21.379755169691776 20.711573311296338 36.20654073404357 21.702130784968322 5 29.252585816484 19.980461620349 30.84195828113407 19.92499428203293 6 29.284497465470093 20.577103825495094 30.779263565869925 19.359135143164885 7 29.080585364161394 26.15386462856119 24.762122006795735 20.003428000481676 8 26.52875423037831 21.842556374374123 30.787419382779785 20.84127001246778 9 22.803497294348045 21.022204614602348 34.40826295999336 21.76603513105625 10-11 27.798098033005903 21.88087912150228 28.091104991236644 22.229917854255174 12-13 26.72210808212861 25.892482718918032 24.509821036178316 22.875588162775045 14-15 26.38624147600383 23.566032818295625 26.066026980798863 23.981698724901683 16-17 25.85160101764093 23.38855404689952 25.449882362856783 25.309962572602764 18-19 25.819103647368053 24.01160060714414 26.222127203835715 23.947168541652097 20-21 24.563199015283892 24.68235789248959 26.563411947911007 24.191031144315506 22-23 24.739634433148332 24.712987649552154 25.835101886420492 24.712276030879025 24-25 25.143646896603254 24.412064004315965 25.763797535153127 24.68049156392765 26-27 24.984458254387604 24.521794580987198 25.912518124160506 24.581229040464688 28-29 25.07358095383755 24.86686962809557 25.210158923630054 24.849390494436825 30-31 25.44548381757798 24.747574875811814 25.11573764565853 24.691203660951675 32-33 25.253386276278604 25.232379627203 24.94518868512998 24.569045411388412 34-35 25.227042487302885 25.241925324295185 24.91568430861967 24.615347879782256 36-37 25.1922334342891 25.142224873902126 25.102240263974036 24.56330142783474 38-39 25.160508882571957 24.82118544895896 25.251203253688708 24.767102414780375 40-41 24.860144965085308 25.20034055141619 24.887899694230953 25.05161478926755 42-43 24.756762430574934 24.97776846726654 25.273948913424977 24.991520188733553 44-45 25.226192111668077 24.90289015856312 25.15738529885958 24.713532430909225 46-47 24.627566782248007 25.00671787870451 25.237704474781435 25.12801086426604 48-49 25.01444156158408 24.789047837730084 25.102457315903692 25.09405328478215 50-51 24.73908447400519 24.95434880060048 25.70179553739284 24.604771188001486 52-53 24.7646398730675 25.110127933334635 25.61877981374693 24.506452379850934 54-55 24.702355501580353 24.842153671343585 25.230970284812848 25.224520542263217 56-57 24.746154387219406 25.20261803874513 25.395954450663087 24.65527312337238 58-59 24.565180466819754 25.364105065292335 25.58222486814317 24.48848959974474 60-61 25.308483838280583 25.00117387784437 24.918531209568457 24.771811074306594 62-63 24.74245412054643 25.308354945197514 25.468125167851994 24.481065766404058 64-65 24.957297667928195 25.22979913980646 25.452997520360874 24.35990567190447 66-67 25.1634611264688 25.282457462153523 25.28757077059393 24.266510640783743 68-69 25.029788525659008 25.09492398851369 25.807473975289174 24.067813510538123 70-71 24.83971671050546 25.647175118836813 25.209245900175436 24.303862270482288 72-73 24.935032844575854 25.179327252921723 25.42577625165438 24.459863650848042 74-75 24.790956498392998 25.236526890415472 25.3215336834795 24.65098292771203 76-77 24.81796232094867 24.960411659232506 25.266670506176524 24.954955513642297 78-79 24.91687596173242 24.940219413434132 25.094530276487436 25.048374348346016 80-81 24.64062829278414 24.840186040036585 25.538471366195832 24.980714300983443 82-83 24.425452572582724 25.494181103421386 25.339085954091466 24.74128036990442 84-85 24.734216110762684 25.221081025042974 25.167719491702623 24.876983372491722 86-87 24.43919785576501 25.518532637597197 25.37671683033636 24.66555267630143 88-89 24.681772962171742 26.432588147967824 24.584675202098406 24.300963687762028 90-91 24.69928235427297 25.400361936486977 24.847793853218008 25.05256185602205 92-93 24.419365833367927 25.77668415193413 24.877703171461505 24.92624684323644 94-95 24.635750647869838 25.903323879085505 24.575602924482855 24.88532254856181 96-97 24.478678501477535 25.258635338685348 24.903163966564072 25.359522193273044 98-99 24.712488079092694 25.17451263105353 25.075798275105004 25.03720101474877 100-101 25.13562507001459 25.792494012384587 24.37459422215575 24.697286695445072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1524.0 1 1373.5 2 1509.0 3 1849.5 4 2035.0 5 2138.5 6 2113.0 7 2208.0 8 2354.5 9 2456.5 10 2622.0 11 2849.5 12 3074.0 13 3307.5 14 3542.0 15 3838.0 16 4171.0 17 4485.0 18 5021.0 19 5627.0 20 6257.5 21 7036.0 22 8098.5 23 9773.5 24 11629.0 25 13658.5 26 16009.5 27 18328.0 28 21224.0 29 25750.0 30 31656.0 31 37984.5 32 46364.0 33 58642.0 34 73334.5 35 90264.0 36 110177.0 37 133407.0 38 161423.0 39 196857.0 40 249746.0 41 333396.5 42 411917.0 43 452161.0 44 527398.5 45 653538.5 46 796427.5 47 975757.5 48 1186940.5 49 1280785.0 50 1236121.0 51 1191785.5 52 1153986.5 53 1122730.5 54 1040131.0 55 926308.0 56 826772.0 57 662928.0 58 494953.5 59 383028.0 60 294593.5 61 215965.0 62 152219.5 63 102495.0 64 59660.0 65 35578.5 66 22757.5 67 15286.5 68 11740.5 69 9093.5 70 6916.5 71 5312.0 72 4021.0 73 2884.5 74 2032.5 75 1438.5 76 991.0 77 685.5 78 484.5 79 326.0 80 226.0 81 153.5 82 92.5 83 55.5 84 39.0 85 27.5 86 14.0 87 12.0 88 12.5 89 7.0 90 3.0 91 1.5 92 3.0 93 4.0 94 1.5 95 2.0 96 2.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16189880315749644 2 0.0 3 0.0 4 0.08316487058936814 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5851771391173327E-4 12-13 3.89166451049921E-5 14-15 0.0 16-17 3.947259717792056E-4 18-19 0.0 20-21 0.0014009992237797157 22-23 2.779760364642293E-6 24-25 0.0 26-27 6.782615289727196E-4 28-29 2.779760364642293E-6 30-31 8.061305057462649E-4 32-33 0.0 34-35 0.0 36-37 0.0013426242561222275 38-39 0.005170354278234666 40-41 0.0057707825169974 42-43 0.0017957251955589212 44-45 0.0031744863364214985 46-47 0.019094173944727913 48-49 0.009704143432966245 50-51 0.008892453406490695 52-53 0.012064159982547553 54-55 0.010829946380646376 56-57 0.015049622614173374 58-59 0.021823898622806644 60-61 0.010565869146005356 62-63 0.00392780139523956 64-65 0.013834867334824693 66-67 0.025993539169770083 68-69 0.030054769062512476 70-71 0.0140127719981618 72-73 0.012814695281000973 74-75 0.024914992148288875 76-77 0.041343375903324826 78-79 0.007727733813705575 80-81 0.0021598738033270616 82-83 0.0025657188165648364 84-85 0.012767439354802053 86-87 0.014265730191344249 88-89 0.017929454351942792 90-91 0.009373351949573812 92-93 0.01865497180711443 94-95 0.026513354357958194 96-97 0.017534728380163584 98-99 0.007947334882512317 100-101 0.3216877681982294 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7987162E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.22857022747248 #Duplication Level Percentage of deduplicated Percentage of total 1 79.45446096697533 20.839769093582834 2 10.027869278790273 5.26033347221329 3 3.1303313255689025 2.4631234502382298 4 1.5986178452137159 1.6771784168031472 5 1.0321841406637124 1.353635711054076 6 0.5882808773965617 0.9257859783764909 7 0.4539260566885162 0.8334082019154082 8 0.3696920624499747 0.7757195378006643 9 0.28944726994838377 0.6832609242292232 >10 2.1897315249184914 11.801996349161922 >50 0.3781654850607783 6.902253175962084 >100 0.40181166995394363 23.544968388177352 >500 0.05351753621943137 9.574284677251379 >1k 0.03165260848249131 12.78910115390817 >5k 2.9004149920012655E-4 0.5074552799190635 >10k+ 2.1310170084449828E-5 0.06772618940668973 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2786897677354549E-4 0.0 5.559520729284586E-6 2 1.3342849750283008E-4 0.0 5.559520729284586E-6 3 1.3898801823211465E-4 0.0 5.559520729284586E-6 4 1.3898801823211465E-4 0.0 5.559520729284586E-6 5 1.5010705969068384E-4 0.0 1.1119041458569172E-5 6 1.61226101149253E-4 0.0 1.1119041458569172E-5 7 1.723451426078222E-4 0.0 1.1119041458569172E-5 8 1.7790466333710676E-4 0.0 1.1119041458569172E-5 9 1.8902370479567595E-4 0.0 1.667856218785376E-5 10-11 2.1404154807745657E-4 2.779760364642293E-6 1.667856218785376E-5 12-13 2.724165157349447E-4 1.1119041458569172E-5 1.667856218785376E-5 14-15 3.891664510499211E-4 1.1119041458569172E-5 1.667856218785376E-5 16-17 5.086961467295396E-4 1.1119041458569172E-5 1.667856218785376E-5 18-19 6.421246442323697E-4 1.3898801823211466E-5 1.667856218785376E-5 20-21 8.422673904866149E-4 1.667856218785376E-5 1.667856218785376E-5 22-23 0.0010729875007519252 1.667856218785376E-5 2.2238082917138345E-5 24-25 0.0012842492884647394 1.9458322552496054E-5 2.2238082917138345E-5 26-27 0.001534427721282546 2.2238082917138345E-5 2.2238082917138345E-5 28-29 0.001784606154100352 2.2238082917138345E-5 2.7797603646422932E-5 30-31 0.002076480992387793 3.057736401106523E-5 2.7797603646422932E-5 32-33 0.0024322903190620066 3.335712437570752E-5 2.7797603646422932E-5 34-35 0.0028687126963108465 3.335712437570752E-5 2.7797603646422932E-5 36-37 0.003460801653979655 3.891664510499211E-5 2.7797603646422932E-5 38-39 0.00402509300800204 4.16964054696344E-5 2.7797603646422932E-5 40-41 0.004783967587549387 4.447616583427669E-5 2.7797603646422932E-5 42-43 0.0057151873097045554 4.447616583427669E-5 2.7797603646422932E-5 44-45 0.006827091455561472 4.447616583427669E-5 2.7797603646422932E-5 46-47 0.008208632356788693 5.003568656356128E-5 2.7797603646422932E-5 48-49 0.00970970295369553 5.281544692820357E-5 2.7797603646422932E-5 50-51 0.011572142398005867 5.5595207292845865E-5 2.7797603646422932E-5 52-53 0.013784831648261133 5.5595207292845865E-5 2.7797603646422932E-5 54-55 0.01671469907259411 5.5595207292845865E-5 2.7797603646422932E-5 56-57 0.020308929224076594 5.5595207292845865E-5 2.7797603646422932E-5 58-59 0.024725968443493198 5.5595207292845865E-5 2.7797603646422932E-5 60-61 0.03048563191903203 5.8374967657488155E-5 2.7797603646422932E-5 62-63 0.0375823601299638 6.115472802213045E-5 2.7797603646422932E-5 64-65 0.04657766466994627 6.115472802213045E-5 3.057736401106523E-5 66-67 0.057657789483410445 6.115472802213045E-5 3.335712437570752E-5 68-69 0.07128695455125161 6.393448838677274E-5 3.335712437570752E-5 70-71 0.08853814737422169 6.671424875141504E-5 3.335712437570752E-5 72-73 0.10987280817285128 6.671424875141504E-5 3.335712437570752E-5 74-75 0.13711167998598112 7.227376948069962E-5 3.335712437570752E-5 76-77 0.17004906054662766 7.505352984534192E-5 3.335712437570752E-5 78-79 0.21114225801713465 8.33928109392688E-5 3.335712437570752E-5 80-81 0.2614114444513259 8.33928109392688E-5 3.335712437570752E-5 82-83 0.32405334426854 8.33928109392688E-5 3.891664510499211E-5 84-85 0.40051065309802625 8.33928109392688E-5 4.447616583427669E-5 86-87 0.4936215062720845 8.33928109392688E-5 5.003568656356128E-5 88-89 0.6060600332615006 8.33928109392688E-5 5.003568656356128E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 170160 0.0 71.82293 1 TTTGTGT 185970 0.0 65.78526 2 TTGTGTT 207725 0.0 60.352764 3 TAACTTT 18615 0.0 60.16145 1 AACTTTG 19500 0.0 57.9237 2 TGTGTTT 221410 0.0 56.890724 4 GTGTTTG 239695 0.0 52.53724 5 ACTTTGT 41130 0.0 50.707603 1 TTTGAGG 71045 0.0 37.947487 8 GTTTGAG 194275 0.0 36.714027 7 TGTTTGA 344470 0.0 36.55746 6 TTGAGGG 36540 0.0 34.655777 9 TTTGAGC 53520 0.0 33.80991 8 TTGAGGC 14235 0.0 33.455273 9 GTTTGAC 63615 0.0 32.146706 7 TTGAGTC 25515 0.0 31.082972 4 TTGAGGT 22080 0.0 30.815432 9 TTTGAGT 75040 0.0 29.547018 3 TTTGACC 18860 0.0 28.826038 8 TTTGATG 35910 0.0 27.819637 8 >>END_MODULE