FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_30515.34100000035be7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_30515.34100000035be7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17987162
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1701600.071.822931
TTTGTGT1859700.065.785262
TTGTGTT2077250.060.3527643
TAACTTT186150.060.161451
AACTTTG195000.057.92372
TGTGTTT2214100.056.8907244
GTGTTTG2396950.052.537245
ACTTTGT411300.050.7076031
TTTGAGG710450.037.9474878
GTTTGAG1942750.036.7140277
TGTTTGA3444700.036.557466
TTGAGGG365400.034.6557779
TTTGAGC535200.033.809918
TTGAGGC142350.033.4552739
GTTTGAC636150.032.1467067
TTGAGTC255150.031.0829724
TTGAGGT220800.030.8154329
TTTGAGT750400.029.5470183
TTTGACC188600.028.8260388
TTTGATG359100.027.8196378
Produced by FastQC (version 0.11.2)