##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_30455.34100000035ba4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13939697 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.745138362763555 33.0 33.0 33.0 33.0 33.0 2 32.7719905246147 33.0 33.0 33.0 33.0 33.0 3 32.79831290450574 33.0 33.0 33.0 33.0 33.0 4 36.71992576309227 37.0 37.0 37.0 37.0 37.0 5 36.72938099013199 37.0 37.0 37.0 37.0 37.0 6 36.67560543102192 37.0 37.0 37.0 37.0 37.0 7 36.639200837722655 37.0 37.0 37.0 37.0 37.0 8 36.63133036535873 37.0 37.0 37.0 37.0 37.0 9 36.6215384738994 37.0 37.0 37.0 37.0 37.0 10-11 36.615785766362066 37.0 37.0 37.0 37.0 37.0 12-13 36.582723390616025 37.0 37.0 37.0 37.0 37.0 14-15 38.67921888833021 40.0 37.0 40.0 37.0 40.0 16-17 38.645638495585665 40.0 37.0 40.0 37.0 40.0 18-19 38.60294459771974 40.0 37.0 40.0 37.0 40.0 20-21 38.55144516412373 40.0 37.0 40.0 37.0 40.0 22-23 38.51358609157717 40.0 37.0 40.0 37.0 40.0 24-25 38.47811021286905 40.0 37.0 40.0 37.0 40.0 26-27 38.38710231649942 40.0 37.0 40.0 37.0 40.0 28-29 38.32557192598949 40.0 37.0 40.0 37.0 40.0 30-31 38.27159969832917 40.0 37.0 40.0 37.0 40.0 32-33 38.183631215226555 40.0 37.0 40.0 37.0 40.0 34-35 38.099550083477425 40.0 37.0 40.0 37.0 40.0 36-37 38.00227745265912 40.0 37.0 40.0 37.0 40.0 38-39 37.87280713490401 40.0 37.0 40.0 33.0 40.0 40-41 37.740407126496365 40.0 37.0 40.0 33.0 40.0 42-43 37.60699371729529 40.0 37.0 40.0 33.0 40.0 44-45 37.43729261116651 40.0 37.0 40.0 33.0 40.0 46-47 37.23164538655324 37.0 37.0 40.0 33.0 40.0 48-49 36.98787918417452 37.0 37.0 40.0 33.0 40.0 50-51 36.770608966608094 37.0 37.0 40.0 33.0 40.0 52-53 36.52206644089897 37.0 37.0 40.0 33.0 40.0 54-55 36.22666009167918 37.0 37.0 40.0 33.0 40.0 56-57 35.962677129926135 37.0 37.0 40.0 33.0 40.0 58-59 35.67106189610864 37.0 35.0 37.0 33.0 40.0 60-61 35.37683136154251 37.0 33.0 37.0 33.0 40.0 62-63 35.111548766088674 37.0 33.0 37.0 33.0 40.0 64-65 34.82288248446146 37.0 33.0 37.0 27.0 38.5 66-67 34.580975540573085 37.0 33.0 37.0 27.0 37.0 68-69 34.32943158664066 37.0 33.0 37.0 27.0 37.0 70-71 34.09293347624414 37.0 33.0 37.0 27.0 37.0 72-73 33.900860327164935 37.0 33.0 37.0 27.0 37.0 74-75 33.651625282816404 37.0 33.0 37.0 27.0 37.0 76-77 30.802375152056747 33.0 30.0 33.0 24.5 37.0 78-79 33.44778534282345 35.0 33.0 37.0 27.0 37.0 80-81 33.976777759229634 37.0 33.0 37.0 27.0 37.0 82-83 33.95394630887601 37.0 33.0 37.0 27.0 37.0 84-85 33.88641116087387 37.0 33.0 37.0 27.0 37.0 86-87 33.73034055187857 37.0 33.0 37.0 27.0 37.0 88-89 33.580373949304644 37.0 33.0 37.0 27.0 37.0 90-91 33.496425819011705 37.0 33.0 37.0 27.0 37.0 92-93 33.40179452968023 37.0 33.0 37.0 27.0 37.0 94-95 33.306145320088376 37.0 33.0 37.0 27.0 37.0 96-97 33.18597868375475 35.0 33.0 37.0 27.0 37.0 98-99 33.00761770503333 33.0 33.0 37.0 27.0 37.0 100-101 30.975357175984527 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10566139012558295 2306 2 0.09851885268273008 2306 3 0.054243515533507036 2306 4 0.07086263422570482 2306 5 0.0688805806255246 2306 6 0.07337178407208711 2306 7 0.10829235715538488 2306 8 0.11770365426500007 2306 9 0.1120409908050064 2306 10-11 0.12029962671272187 2306 12-13 0.10736581097950193 2306 14-15 0.15437116741544088 2306 16-17 0.16427203619284114 2306 18-19 0.18361935106238292 2306 20-21 0.20740335205146465 2306 22-23 0.12340876124906686 2306 24-25 0.14404795330768394 2306 26-27 0.14695919705863503 2306 28-29 0.0881220392302069 2306 30-31 0.06761201766612146 2306 32-33 0.10284984820196286 2306 34-35 0.0863971129873704 2306 36-37 0.08972382019832281 2306 38-39 0.013605315946790597 2306 40-41 0.04563459103820122 2306 42-43 0.094022463191763 2306 44-45 0.04564360529501954 2306 46-47 0.05507988504992767 2306 48-49 3.2896270715099263E-4 2306 50-51 -0.010573635636234258 2306 52-53 -0.017697963493610303 2306 54-55 0.00662471871481074 2306 56-57 -0.0657396622161528 2306 58-59 -0.03611541236096372 2306 60-61 -0.14901597980033898 2306 62-63 -0.22712934941044693 2306 64-65 -0.17832099759726106 2306 66-67 -0.2342905530218431 2306 68-69 -0.2700516667315256 2306 70-71 -0.2871833219046209 2306 72-73 -0.35013974615880983 2306 74-75 -0.431960241346637 2306 76-77 -0.3015542867814105 2306 78-79 -0.22137656568515496 2306 80-81 -0.19219969601318354 2306 82-83 -0.22739526478250838 2306 84-85 -0.2750490371515468 2306 86-87 -0.20733633042230792 2306 88-89 -0.37931863199025173 2306 90-91 -0.4482660347886096 2306 92-93 -0.3452621403203011 2306 94-95 -0.40034635380246897 2306 96-97 -0.4874084214875012 2306 98-99 -0.37622177100019627 2306 100-101 -0.7461124224612696 2307 1 0.11054477605578228 2307 2 0.10927309417470354 2307 3 0.0969260548350519 2307 4 0.06670649041084431 2307 5 0.07331464078353633 2307 6 0.06484396060832154 2307 7 0.09852461127894685 2307 8 0.06361955285044729 2307 9 0.07244838692619737 2307 10-11 0.07890778040967206 2307 12-13 0.10794031955362016 2307 14-15 0.13515993603653698 2307 16-17 0.13030633599358055 2307 18-19 0.17168057127489078 2307 20-21 0.1861121224744906 2307 22-23 0.1493683757613553 2307 24-25 0.10177347019711647 2307 26-27 0.12724693009197097 2307 28-29 0.11495837688121213 2307 30-31 0.05487175313032111 2307 32-33 0.03442346937791285 2307 34-35 0.010156799228973057 2307 36-37 0.017695173517076057 2307 38-39 -0.008833895886397158 2307 40-41 -0.03882414825110203 2307 42-43 -0.012450325583131416 2307 44-45 -0.07989670401073568 2307 46-47 -0.04185993919583808 2307 48-49 -0.03310091561737494 2307 50-51 -0.06727970559980179 2307 52-53 -0.17210650356184942 2307 54-55 -0.10263340370926954 2307 56-57 -0.12633406506779465 2307 58-59 -0.1833567815353021 2307 60-61 -0.26278887961304775 2307 62-63 -0.221206857953959 2307 64-65 -0.2926053558389654 2307 66-67 -0.3098604915475178 2307 68-69 -0.35720961752005564 2307 70-71 -0.40174043942244 2307 72-73 -0.49555630498799275 2307 74-75 -0.6093436977190692 2307 76-77 -0.8202660392588896 2307 78-79 -0.41021332877244987 2307 80-81 -0.2914849900060048 2307 82-83 -0.34947256878265165 2307 84-85 -0.45171211735202377 2307 86-87 -0.3765307513280334 2307 88-89 -0.40836333358651444 2307 90-91 -0.4671581976468744 2307 92-93 -0.40538201908313454 2307 94-95 -0.437260691875089 2307 96-97 -0.402518636619817 2307 98-99 -0.38898272902845576 2307 100-101 -0.6392445353872986 2201 1 0.0874871557371506 2201 2 0.07405215407372623 2201 3 0.07381992300519613 2201 4 0.02789381516798528 2201 5 0.04795425793160035 2201 6 0.06626099841118105 2201 7 0.07334995380605847 2201 8 0.10801683981983246 2201 9 0.07309414365410305 2201 10-11 0.08238214139816336 2201 12-13 0.11122522101059928 2201 14-15 0.16253298648118886 2201 16-17 0.16462441891022905 2201 18-19 0.1359711431479198 2201 20-21 0.15227024141044154 2201 22-23 0.1535771917754971 2201 24-25 0.10845890601294172 2201 26-27 0.10858547876994606 2201 28-29 0.1097955539116171 2201 30-31 0.07524767967603907 2201 32-33 0.12700276108176212 2201 34-35 0.09697949307302878 2201 36-37 0.10834902661813572 2201 38-39 0.09558528117182874 2201 40-41 0.1665063958002193 2201 42-43 0.13845535616283655 2201 44-45 0.11939195293209082 2201 46-47 0.02724789068303579 2201 48-49 0.04304228075235983 2201 50-51 -0.001539918894430059 2201 52-53 -0.02875888847533048 2201 54-55 0.0441189304221794 2201 56-57 -0.022886973133601884 2201 58-59 -0.04840951990417608 2201 60-61 -0.08370846953459221 2201 62-63 -0.12822976536394037 2201 64-65 -0.15284142311335813 2201 66-67 -0.14636616193516971 2201 68-69 -0.1733052019846184 2201 70-71 -0.18516860683426728 2201 72-73 -0.17472168327058313 2201 74-75 -0.3348231359047915 2201 76-77 -0.5325530171238171 2201 78-79 -0.19745443115368033 2201 80-81 -0.15460086813616414 2201 82-83 -0.0767345330619591 2201 84-85 -0.15214171211115968 2201 86-87 -0.07954486665978067 2201 88-89 -0.051210645301559055 2201 90-91 -0.15630633737693955 2201 92-93 -0.16071894082334381 2201 94-95 -0.09360400991813123 2201 96-97 -0.22272079843919812 2201 98-99 -0.15450141007190155 2201 100-101 -0.332927206639571 1101 1 0.056268585920946634 1101 2 0.07146380122081553 1101 3 0.07432237204557879 1101 4 0.04449551400524143 1101 5 0.052083726013265164 1101 6 0.06869232499631295 1101 7 0.07395340267778039 1101 8 0.08407145298941288 1101 9 0.08033280491580541 1101 10-11 0.06125826798246692 1101 12-13 0.08219235209683973 1101 14-15 0.14048773700384487 1101 16-17 0.11735095990289324 1101 18-19 0.07483163211438892 1101 20-21 0.0701274051470051 1101 22-23 0.10684804558398042 1101 24-25 0.10122846602180857 1101 26-27 0.09041316708964331 1101 28-29 0.08399450071205905 1101 30-31 0.09173125103494328 1101 32-33 0.04876425949540675 1101 34-35 0.07221367292014946 1101 36-37 0.011260400042580443 1101 38-39 -0.003975618483636367 1101 40-41 -0.007791592951193138 1101 42-43 -0.013963136866699699 1101 44-45 -0.07694584631721568 1101 46-47 -0.06803292797525984 1101 48-49 -0.02674942863826857 1101 50-51 -0.03502911990537427 1101 52-53 -0.023933398492935964 1101 54-55 -0.04443102364680129 1101 56-57 -0.05901061559249143 1101 58-59 -0.1057132487162491 1101 60-61 -0.19973871698965695 1101 62-63 -0.17333176605885114 1101 64-65 -0.11671937923485132 1101 66-67 -0.17190889976868107 1101 68-69 -0.19136058892799923 1101 70-71 -0.24086591840875826 1101 72-73 -0.23316279441610277 1101 74-75 -0.34265618741601855 1101 76-77 -0.8892746700839496 1101 78-79 -0.26188522509182377 1101 80-81 -0.0958883599084217 1101 82-83 -0.06923659728597187 1101 84-85 -0.05457772482051837 1101 86-87 -0.01298627849065781 1101 88-89 -0.11850081617275521 1101 90-91 -0.1607911851033279 1101 92-93 -0.023285429968424864 1101 94-95 -0.052631998746583974 1101 96-97 -0.2130771719441853 1101 98-99 -0.11687293254676945 1101 100-101 -0.19598753532779867 2304 1 0.0827648461694892 2304 2 0.07976926399599904 2304 3 0.04272867408527503 2304 4 0.043513402685448455 2304 5 0.05418059252197338 2304 6 0.04414285090479808 2304 7 0.07097282876084421 2304 8 0.07308318883556097 2304 9 0.06187355466613553 2304 10-11 0.08846325219237627 2304 12-13 0.07950615549549411 2304 14-15 0.1283171297112986 2304 16-17 0.08378680993257603 2304 18-19 0.10674216477418241 2304 20-21 0.1067767479297217 2304 22-23 0.10849644058164643 2304 24-25 0.06378315931867462 2304 26-27 0.0710748418965963 2304 28-29 0.040818245150077814 2304 30-31 0.03771259641552405 2304 32-33 0.0674526872759671 2304 34-35 0.06414781791502833 2304 36-37 0.06846036392661858 2304 38-39 0.04959204698081976 2304 40-41 0.06319652848821278 2304 42-43 0.07758094307916963 2304 44-45 0.013842350599716724 2304 46-47 0.05989550913238162 2304 48-49 0.04515446779983989 2304 50-51 -0.041231803305237236 2304 52-53 -0.10184242240784869 2304 54-55 3.1632805073655845E-4 2304 56-57 -0.03619191380825271 2304 58-59 0.006829521018950402 2304 60-61 -0.0624535209316619 2304 62-63 -0.05385678603023791 2304 64-65 -0.11703644411894487 2304 66-67 -0.15464157409120816 2304 68-69 -0.1571391037056742 2304 70-71 -0.16080964633333394 2304 72-73 -0.1519219349022407 2304 74-75 -0.26547861819059193 2304 76-77 -0.8896591071197761 2304 78-79 -0.24765427870746493 2304 80-81 -0.051010579868439265 2304 82-83 -0.06563630891071881 2304 84-85 -0.10273415343296932 2304 86-87 0.021317238764382296 2304 88-89 -0.14998071662712675 2304 90-91 -0.10371691639113578 2304 92-93 -0.045155800021220216 2304 94-95 -0.10295737041700193 2304 96-97 -0.12356510399921916 2304 98-99 -0.0718219529904971 2304 100-101 -0.181291745553537 2202 1 0.045753388508899206 2202 2 0.04484723491099629 2202 3 0.02856313906222141 2202 4 0.029251541187321095 2202 5 0.031174243506775667 2202 6 0.022138813507623922 2202 7 0.03157388748798695 2202 8 0.027488186312872642 2202 9 0.027869493791932598 2202 10-11 0.047585230801757916 2202 12-13 0.018171339015225385 2202 14-15 0.0283746620231895 2202 16-17 0.06129801532377144 2202 18-19 0.06807435367471726 2202 20-21 0.03856472382054932 2202 22-23 0.07396301885439982 2202 24-25 0.026654547469867396 2202 26-27 0.014088751385251896 2202 28-29 -0.007748476680369265 2202 30-31 -0.013941450078995388 2202 32-33 -0.05421216137000329 2202 34-35 -0.04327285852344431 2202 36-37 -0.007602145624510115 2202 38-39 0.025651331157448 2202 40-41 -0.0077791823495445556 2202 42-43 0.021437389947934093 2202 44-45 -0.011864441174886053 2202 46-47 -0.002034833212725573 2202 48-49 0.027392857592900555 2202 50-51 -0.05481939306412187 2202 52-53 -0.01314785727527834 2202 54-55 -0.003246161936203862 2202 56-57 -0.04007743034966893 2202 58-59 -0.09474318734568499 2202 60-61 -0.15603353663084363 2202 62-63 -0.20126983732472326 2202 64-65 -0.12890726240868133 2202 66-67 -0.14120977780054034 2202 68-69 -0.1711077196977513 2202 70-71 -0.1141479605651341 2202 72-73 -0.12779766891269873 2202 74-75 -0.30274949160787656 2202 76-77 -0.5447678965288532 2202 78-79 -0.2070333634119308 2202 80-81 -0.034976202539077406 2202 82-83 -1.2509438956698204E-4 2202 84-85 0.0017003615196244937 2202 86-87 0.0056047467449431565 2202 88-89 -0.05063866585195598 2202 90-91 -0.14401687024138 2202 92-93 -0.05849451694931673 2202 94-95 -0.10643332961131335 2202 96-97 -0.09424477871626635 2202 98-99 -0.11337052348930143 2202 100-101 -0.04277655689277182 1102 1 0.052686371578531066 1102 2 0.05731818070875505 1102 3 0.031189351006879917 1102 4 0.025755132656342994 1102 5 0.03575215655430242 1102 6 0.00840645279319574 1102 7 0.019732321773311412 1102 8 0.07522626740202298 1102 9 0.04577593915860234 1102 10-11 0.030813487067689493 1102 12-13 0.022082986263413318 1102 14-15 0.07859896802035848 1102 16-17 0.010965043240716454 1102 18-19 0.03169482686561764 1102 20-21 0.05908801292573429 1102 22-23 0.051214359605509685 1102 24-25 0.05623862770704591 1102 26-27 0.06386323032919705 1102 28-29 0.05055311196318257 1102 30-31 -0.001777944811763632 1102 32-33 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1213 50-51 -0.615342378991798 1213 52-53 -0.2886774881911549 1213 54-55 -0.7939376835405696 1213 56-57 -0.3328828476585741 1213 58-59 -0.1646508848738577 1213 60-61 -0.15743354923196762 1213 62-63 -0.0012444173484595922 1213 64-65 -0.015175100565180344 1213 66-67 -0.40017592163989235 1213 68-69 -0.04539235891722626 1213 70-71 -0.02678712395088212 1213 72-73 0.05269067460434229 1213 74-75 0.09324193352617982 1213 76-77 0.6434554657160163 1213 78-79 -0.005705013434614159 1213 80-81 -0.18175533961211698 1213 82-83 -0.11377442771510715 1213 84-85 -0.13297299622620073 1213 86-87 -1.3805250072016975 1213 88-89 -0.6868821852160423 1213 90-91 -0.3404341917862439 1213 92-93 -0.2487479717426737 1213 94-95 0.014203427864586615 1213 96-97 0.15158698890812872 1213 98-99 0.054507079918387547 1213 100-101 0.48531795206537964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 23.0 8 295.0 9 893.0 10 647.0 11 230.0 12 202.0 13 375.0 14 952.0 15 2053.0 16 3810.0 17 5999.0 18 8448.0 19 11791.0 20 15985.0 21 20925.0 22 27080.0 23 35532.0 24 46038.0 25 58854.0 26 76768.0 27 98401.0 28 126245.0 29 163362.0 30 211027.0 31 278928.0 32 381380.0 33 553773.0 34 892537.0 35 1699282.0 36 3744715.0 37 4809112.0 38 663997.0 39 36.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.80655626391037 21.30073344362831 31.040175034893807 27.852535257567514 2 27.640277977347715 21.981639916563466 31.215764589431178 19.16231751665764 3 20.93989560892177 20.081125149276918 38.84451720865955 20.13446203314175 4 21.006345264586574 20.151423925965357 37.60726274254601 21.234968066902056 5 29.78684543860602 19.43159166228649 31.40121338361946 19.380349515488035 6 29.653420730737544 20.06285359000271 31.501631635178295 18.782094044081447 7 29.640816439553884 26.498703666227463 24.39102514208164 19.469454752137008 8 26.505145700082288 21.459067582315456 31.643837021708578 20.391949695893675 9 22.535174186354265 20.578618028784987 35.413302025144446 21.472905759716298 10-11 28.254624870858574 21.50886110238202 28.211255805782052 22.02525822097735 12-13 26.876743537571958 25.97364245735433 24.382362564564737 22.767251440508975 14-15 26.52625806715885 23.450237117779533 26.066714362586218 23.956790452475403 16-17 25.95545320007752 23.238129754220235 25.37350907800477 25.432907967697478 18-19 25.925348305633904 23.96728924595707 26.241190895325772 23.866171553083255 20-21 24.524211704036976 24.711366937317255 26.60540573268851 24.159015625957263 22-23 24.745757556486257 24.70831413008167 25.81708288986062 24.728845423571453 24-25 25.170575802329132 24.355070271613506 25.776040899597746 24.698313026459616 26-27 25.006247484461635 24.503693987719384 25.90131194842209 24.58874657939689 28-29 25.08533363386593 24.87469777858156 25.1334695438502 24.90649904370231 30-31 25.524501222255257 24.750139001472817 25.01169240825804 24.713667368013883 32-33 25.269462456752105 25.26318183243151 24.872502608916104 24.594853101900277 34-35 25.278332807377378 25.286284917096836 24.811027097647816 24.62435517787797 36-37 25.229721772093317 25.13156563327783 25.045188948504737 24.593523646124112 38-39 25.177455493899558 24.800035698430907 25.202704816160253 24.819803991509286 40-41 24.890401985293952 25.188506720009972 24.766077191352284 25.155014103343788 42-43 24.761607695213385 24.999866386603422 25.19327401998519 25.045251898198 44-45 25.249872401804268 24.890986895327224 25.121906539616923 24.737234163251586 46-47 24.568105769986225 25.007976947751082 25.225211101213425 25.198706181049268 48-49 25.029790143514276 24.769217413701135 25.04220554320154 25.158786899583056 50-51 24.727930008120723 24.946423255955104 25.725672434681783 24.599974301242387 52-53 24.7332253310738 25.089467770796052 25.66142654817987 24.51588034995028 54-55 24.705257366153408 24.812297906677465 25.163775004435657 25.318669722733468 56-57 24.72014280840506 25.231545274619542 25.36891513611964 24.679396780855754 58-59 24.57527703644515 25.403849887784798 25.554524936975366 24.46634813879469 60-61 25.380772426473726 24.971587038509544 24.87054444191821 24.777096093098518 62-63 24.72607754668788 25.32548939089891 25.49114136256665 24.45729169984656 64-65 24.976461376676017 25.219793666558783 25.46509543052596 24.33864952623924 66-67 25.219607365322982 25.28384742603477 25.277432389533644 24.2191128191086 68-69 25.052299354274076 25.07532344870006 25.866003932502412 24.00637326452345 70-71 24.86996473894869 25.634944300223893 25.20905083873604 24.286040122091375 72-73 24.999638575952662 25.138106137274484 25.438914977387412 24.42334030938544 74-75 24.812472854065682 25.21250876042757 25.307584558800578 24.667433826706166 76-77 24.837428603867494 24.895642835397297 25.270656883891192 24.99627167684402 78-79 24.95603223274614 24.89065632035309 25.07288382924565 25.080427617655122 80-81 24.65209858543783 24.75874950585217 25.56747504546734 25.02167686324266 82-83 24.409027618705796 25.495523215310435 25.334927572340675 24.76052159364309 84-85 24.762091371779164 25.176299695922932 25.168267647511673 24.89334128478623 86-87 24.43541153279952 25.511846524750116 25.428740835809478 24.624001106640893 88-89 24.693554651954088 26.506158001775322 24.530361802089036 24.269925544181554 90-91 24.697510849977856 25.366214217313797 24.85050553713421 25.085769395574133 92-93 24.41938907561747 25.781273991816345 24.85508404795163 24.944252884614556 94-95 24.63993894935193 25.873146971470227 24.573449139170076 24.913464940007763 96-97 24.453562390854362 25.14033099984667 24.960600125118145 25.445506484180818 98-99 24.746550781208803 25.06534555752443 25.13854879592923 25.049554865337537 100-101 25.18102172167101 25.723171985847948 24.384761123799777 24.711045168681263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1267.0 1 1129.0 2 1251.0 3 1509.5 4 1649.0 5 1737.5 6 1645.0 7 1682.0 8 1822.0 9 1905.0 10 1965.0 11 2110.5 12 2348.5 13 2549.5 14 2663.5 15 2836.5 16 3120.0 17 3439.5 18 3775.5 19 4173.5 20 4639.0 21 5160.0 22 6087.0 23 7382.0 24 8670.5 25 10216.0 26 11808.0 27 13567.5 28 15935.5 29 19509.0 30 24018.5 31 28729.0 32 35318.5 33 44864.0 34 56076.5 35 68595.5 36 83833.0 37 102483.5 38 123678.0 39 150694.0 40 192058.5 41 257190.5 42 317394.0 43 348806.5 44 408507.0 45 509761.0 46 623926.0 47 761461.5 48 923064.0 49 996212.0 50 964059.5 51 930984.5 52 900259.5 53 871531.5 54 803832.5 55 714565.0 56 636206.5 57 509804.0 58 381861.5 59 296534.0 60 227412.0 61 165950.0 62 116998.5 63 78532.5 64 45374.5 65 26985.0 66 17389.0 67 11889.0 68 9156.5 69 6929.5 70 5279.0 71 4052.0 72 3015.0 73 2156.0 74 1583.5 75 1180.0 76 806.0 77 562.0 78 385.0 79 249.0 80 176.5 81 122.0 82 86.0 83 61.0 84 39.0 85 24.0 86 16.0 87 12.5 88 8.0 89 7.0 90 6.5 91 4.0 92 5.0 93 4.5 94 1.5 95 3.0 96 3.5 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16120866902630668 2 0.0 3 0.0 4 0.08260581273753655 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.6901589037408777E-4 12-13 4.304254245985404E-5 14-15 0.0 16-17 3.0488467575729946E-4 18-19 0.0 20-21 0.0014275776582518256 22-23 3.5868785383211705E-6 24-25 0.0 26-27 6.205299871295624E-4 28-29 0.0 30-31 6.922675578959858E-4 32-33 0.0 34-35 0.0 36-37 0.0012302993386441613 38-39 0.005086193767339419 40-41 0.005767700689620441 42-43 0.0016248559778594901 44-45 0.003102649935647812 46-47 0.01909654133802191 48-49 0.009770657138386868 50-51 0.009182409058102196 52-53 0.012317340900594897 54-55 0.010904110756496357 56-57 0.015366187658167893 58-59 0.022116693067288334 60-61 0.010875415728189789 62-63 0.0038236125218503673 64-65 0.014103606412678842 66-67 0.026463989855733592 68-69 0.030814873522717175 70-71 0.013909914971609497 72-73 0.012737005689578477 74-75 0.02473511440026279 76-77 0.04174409242898178 78-79 0.007726136371543801 80-81 0.0021198452161478114 82-83 0.0024390774060583957 84-85 0.012790808867653292 86-87 0.014512510566047455 88-89 0.017973848355527386 90-91 0.009358166106479934 92-93 0.01903197752433213 94-95 0.026736592624645998 96-97 0.017719179979306582 98-99 0.007934175326766428 100-101 0.3210148685441298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3939697E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.161555651357933 #Duplication Level Percentage of deduplicated Percentage of total 1 79.72905261748063 22.45294152317226 2 10.355232365304214 5.832389050765149 3 2.979169415441924 2.516941358633737 4 1.5037804504213814 1.693951873678633 5 0.939639470803357 1.3230854624620627 6 0.5359256920545725 0.9055500721072416 7 0.3534672996493173 0.6967932321006621 8 0.3403178844772429 0.7667104834286622 9 0.25675039506176184 0.6507441485135947 >10 2.1626242669799263 12.94543944828433 >50 0.35707276596391885 7.137247538918583 >100 0.41886814554915763 24.966074203007928 >500 0.04754169745338592 9.420364956067385 >1k 0.020243288030065136 8.111613225744788 >5k 2.8864773309281204E-4 0.5027249776247064 >10k+ 2.5597596085866958E-5 0.07742844549025671 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0043259907299277E-4 0.0 0.0 2 1.0043259907299277E-4 0.0 0.0 3 1.076063561496351E-4 0.0 0.0 4 1.1478011322627744E-4 0.0 0.0 5 1.1478011322627744E-4 0.0 0.0 6 1.2195387030291978E-4 0.0 0.0 7 1.3630138445620447E-4 0.0 0.0 8 1.3630138445620447E-4 0.0 0.0 9 1.434751415328468E-4 0.0 0.0 10-11 1.8651768399270083E-4 0.0 0.0 12-13 2.2597334791423372E-4 0.0 0.0 14-15 3.012977972189783E-4 0.0 0.0 16-17 4.2683854606021925E-4 7.17375707664234E-6 0.0 18-19 5.631399305164237E-4 7.17375707664234E-6 0.0 20-21 7.137888291259128E-4 7.17375707664234E-6 0.0 22-23 8.895458775036502E-4 7.17375707664234E-6 0.0 24-25 0.0011083454683412416 7.17375707664234E-6 0.0 26-27 0.001330731937717154 7.17375707664234E-6 0.0 28-29 0.0015710527997846724 7.17375707664234E-6 0.0 30-31 0.0018831112326186143 7.17375707664234E-6 0.0 32-33 0.0022202778152208046 7.17375707664234E-6 0.0 34-35 0.002740375203277374 7.17375707664234E-6 0.0 36-37 0.0032138431703357686 7.17375707664234E-6 0.0 38-39 0.003837960036003652 7.17375707664234E-6 0.0 40-41 0.004454903144594893 7.17375707664234E-6 0.0 42-43 0.005290645844023726 1.434751415328468E-5 0.0 44-45 0.006355948769905114 1.434751415328468E-5 0.0 46-47 0.007460707359708034 1.434751415328468E-5 7.17375707664234E-6 48-49 0.008845242475500005 1.793439269160585E-5 7.17375707664234E-6 50-51 0.010559770416817525 2.152127122992702E-5 7.17375707664234E-6 52-53 0.012579183033892345 2.152127122992702E-5 7.17375707664234E-6 54-55 0.015136627431715337 2.152127122992702E-5 7.17375707664234E-6 56-57 0.01845449007966242 2.152127122992702E-5 7.17375707664234E-6 58-59 0.02226016820882118 3.58687853832117E-5 7.17375707664234E-6 60-61 0.027088106721401474 3.58687853832117E-5 7.17375707664234E-6 62-63 0.032884502439328486 3.58687853832117E-5 7.17375707664234E-6 64-65 0.04030934101365331 3.58687853832117E-5 7.17375707664234E-6 66-67 0.049692615269901486 3.58687853832117E-5 7.17375707664234E-6 68-69 0.06103791208661136 3.58687853832117E-5 7.17375707664234E-6 70-71 0.07601312998410223 3.58687853832117E-5 7.17375707664234E-6 72-73 0.09429903677246357 3.58687853832117E-5 7.17375707664234E-6 74-75 0.11709006300495628 3.58687853832117E-5 7.17375707664234E-6 76-77 0.1455411835709198 3.58687853832117E-5 7.17375707664234E-6 78-79 0.18139920831851652 4.304254245985404E-5 7.17375707664234E-6 80-81 0.22543890301202385 4.304254245985404E-5 7.17375707664234E-6 82-83 0.27879372126955126 4.304254245985404E-5 7.17375707664234E-6 84-85 0.3442291464441444 5.0216299536496384E-5 7.17375707664234E-6 86-87 0.424055128314482 5.0216299536496384E-5 7.17375707664234E-6 88-89 0.5225902686407029 5.0216299536496384E-5 7.17375707664234E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 144840 0.0 73.67748 1 TTTGTGT 158240 0.0 67.55669 2 TAACTTT 15455 0.0 63.84369 1 TTGTGTT 177715 0.0 61.573322 3 AACTTTG 16275 0.0 61.053753 2 TGTGTTT 189140 0.0 58.185627 4 GTGTTTG 205045 0.0 53.702084 5 ACTTTGT 32405 0.0 47.238018 1 TTTGAGG 61475 0.0 38.820713 8 GTTTGAG 168320 0.0 37.39524 7 TGTTTGA 297675 0.0 36.968826 6 TTGAGGG 31470 0.0 36.393192 9 TTTGAGC 45555 0.0 35.095116 8 TTGAGTC 21600 0.0 33.50622 4 TTTGAGT 64055 0.0 32.041252 3 TTGAGGC 12185 0.0 31.993164 9 GTTTGAC 54150 0.0 31.874657 7 TTGAGGT 18925 0.0 31.412899 9 TTTGACC 16195 0.0 29.700796 8 TTGAGCC 27545 0.0 29.563845 9 >>END_MODULE