FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_30455.34100000035ba4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_30455.34100000035ba4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13939697
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1448400.073.677481
TTTGTGT1582400.067.556692
TAACTTT154550.063.843691
TTGTGTT1777150.061.5733223
AACTTTG162750.061.0537532
TGTGTTT1891400.058.1856274
GTGTTTG2050450.053.7020845
ACTTTGT324050.047.2380181
TTTGAGG614750.038.8207138
GTTTGAG1683200.037.395247
TGTTTGA2976750.036.9688266
TTGAGGG314700.036.3931929
TTTGAGC455550.035.0951168
TTGAGTC216000.033.506224
TTTGAGT640550.032.0412523
TTGAGGC121850.031.9931649
GTTTGAC541500.031.8746577
TTGAGGT189250.031.4128999
TTTGACC161950.029.7007968
TTGAGCC275450.029.5638459