##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_30429.34100000035b60.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14661082 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.743028174864584 33.0 33.0 33.0 33.0 33.0 2 32.7703286155824 33.0 33.0 33.0 33.0 33.0 3 32.796980877673285 33.0 33.0 33.0 33.0 33.0 4 36.71784913282662 37.0 37.0 37.0 37.0 37.0 5 36.72635594016867 37.0 37.0 37.0 37.0 37.0 6 36.67354885539826 37.0 37.0 37.0 37.0 37.0 7 36.63631715585521 37.0 37.0 37.0 37.0 37.0 8 36.62780236820175 37.0 37.0 37.0 37.0 37.0 9 36.61805356521435 37.0 37.0 37.0 37.0 37.0 10-11 36.61039140903789 37.0 37.0 37.0 37.0 37.0 12-13 36.57642976145962 37.0 37.0 37.0 37.0 37.0 14-15 38.66794913226732 40.0 37.0 40.0 37.0 40.0 16-17 38.630554484314324 40.0 37.0 40.0 37.0 40.0 18-19 38.58594430479278 40.0 37.0 40.0 37.0 40.0 20-21 38.5315661899988 40.0 37.0 40.0 37.0 40.0 22-23 38.4818650151469 40.0 37.0 40.0 37.0 40.0 24-25 38.43807118055816 40.0 37.0 40.0 37.0 40.0 26-27 38.34397355529421 40.0 37.0 40.0 37.0 40.0 28-29 38.274089934153565 40.0 37.0 40.0 37.0 40.0 30-31 38.22238389363077 40.0 37.0 40.0 37.0 40.0 32-33 38.13857169614084 40.0 37.0 40.0 37.0 40.0 34-35 38.05347593035766 40.0 37.0 40.0 37.0 40.0 36-37 37.95118910732509 40.0 37.0 40.0 35.0 40.0 38-39 37.82178689130856 40.0 37.0 40.0 33.0 40.0 40-41 37.683481035028656 40.0 37.0 40.0 33.0 40.0 42-43 37.54334720998082 40.0 37.0 40.0 33.0 40.0 44-45 37.3764710544556 40.0 37.0 40.0 33.0 40.0 46-47 37.17136692230491 37.0 37.0 40.0 33.0 40.0 48-49 36.92725158347794 37.0 37.0 40.0 33.0 40.0 50-51 36.69805448192705 37.0 37.0 40.0 33.0 40.0 52-53 36.443699619168626 37.0 37.0 40.0 33.0 40.0 54-55 36.14447797918326 37.0 37.0 40.0 33.0 40.0 56-57 35.88945508933106 37.0 37.0 40.0 33.0 40.0 58-59 35.60208417768893 37.0 33.0 37.0 33.0 40.0 60-61 35.304930973034594 37.0 33.0 37.0 33.0 40.0 62-63 35.04721711535342 37.0 33.0 37.0 33.0 40.0 64-65 34.76664761168378 37.0 33.0 37.0 27.0 38.5 66-67 34.52684986005808 37.0 33.0 37.0 27.0 37.0 68-69 34.27824034406191 37.0 33.0 37.0 27.0 37.0 70-71 34.04547321268649 37.0 33.0 37.0 27.0 37.0 72-73 33.84895702104387 37.0 33.0 37.0 27.0 37.0 74-75 33.60076203789052 37.0 33.0 37.0 27.0 37.0 76-77 30.73472234177532 33.0 30.0 33.0 24.5 35.0 78-79 33.394631071567574 35.0 33.0 37.0 27.0 37.0 80-81 33.930151301247754 37.0 33.0 37.0 27.0 37.0 82-83 33.90175169199654 37.0 33.0 37.0 27.0 37.0 84-85 33.83393896166736 37.0 33.0 37.0 27.0 37.0 86-87 33.67855336325108 37.0 33.0 37.0 27.0 37.0 88-89 33.53439903003066 37.0 33.0 37.0 27.0 37.0 90-91 33.447179546502774 37.0 33.0 37.0 27.0 37.0 92-93 33.349974442541146 37.0 33.0 37.0 27.0 37.0 94-95 33.24631101578997 37.0 33.0 37.0 27.0 37.0 96-97 33.129789738574544 33.0 33.0 37.0 27.0 37.0 98-99 32.943956285081825 33.0 33.0 37.0 27.0 37.0 100-101 30.883162852509795 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.082643082180347 2306 2 0.07692477678703824 2306 3 0.06733381908642855 2306 4 0.06872909812045691 2306 5 0.06483232326450405 2306 6 0.07464684499626628 2306 7 0.09680193970265805 2306 8 0.10292005993946418 2306 9 0.07577558052285127 2306 10-11 0.07022333258850466 2306 12-13 0.08999752423166285 2306 14-15 0.160696484363136 2306 16-17 0.15595424642678068 2306 18-19 0.2009014633214079 2306 20-21 0.1564917416505338 2306 22-23 0.16439908060348074 2306 24-25 0.1304669486395582 2306 26-27 0.13314566622198498 2306 28-29 0.11745722519131618 2306 30-31 0.06659019720213877 2306 32-33 0.07782319677455263 2306 34-35 0.06781918266143805 2306 36-37 0.013509938939094468 2306 38-39 0.04492128108498861 2306 40-41 0.029425892362965556 2306 42-43 -0.01876264497374791 2306 44-45 -0.04002730582316616 2306 46-47 -0.006813823469727254 2306 48-49 -0.04483192203746711 2306 50-51 -0.10609540746899171 2306 52-53 -0.11031697125802253 2306 54-55 -0.0850247755055662 2306 56-57 -0.14699302232101275 2306 58-59 -0.1674607124673102 2306 60-61 -0.26219622911110463 2306 62-63 -0.3282615959504298 2306 64-65 -0.29748873263825004 2306 66-67 -0.3141459439714396 2306 68-69 -0.3227219954456828 2306 70-71 -0.2987637875440896 2306 72-73 -0.3608638859233082 2306 74-75 -0.4548362603373022 2306 76-77 -0.3688868811991952 2306 78-79 -0.34460007073833765 2306 80-81 -0.28504023768908837 2306 82-83 -0.30169737419767984 2306 84-85 -0.35981355356121014 2306 86-87 -0.21136516923716897 2306 88-89 -0.4412792072089786 2306 90-91 -0.5422637284736496 2306 92-93 -0.443324427041631 2306 94-95 -0.46420612714774023 2306 96-97 -0.5218036453232031 2306 98-99 -0.4996450292512691 2306 100-101 -0.8392418627485796 2307 1 0.08692071766352427 2307 2 0.08977609662826325 2307 3 0.07610836285884659 2307 4 0.06789512492176186 2307 5 0.07986506738976118 2307 6 0.07200887089491204 2307 7 0.11981724716435593 2307 8 0.066349222700957 2307 9 0.08765723069828368 2307 10-11 0.07888251134713897 2307 12-13 0.0833074275470338 2307 14-15 0.12941878115959327 2307 16-17 0.13251740016611535 2307 18-19 0.19658132359240454 2307 20-21 0.1901830568925078 2307 22-23 0.14422682639310835 2307 24-25 0.09796570113226721 2307 26-27 0.1799095177470278 2307 28-29 0.15206452994748076 2307 30-31 0.054768441698023196 2307 32-33 0.06394065027613038 2307 34-35 0.1044997330254418 2307 36-37 0.08885968596977989 2307 38-39 0.08785537730559412 2307 40-41 0.03789137247066776 2307 42-43 0.040446843569746704 2307 44-45 0.018493879909236455 2307 46-47 0.05485063052103811 2307 48-49 0.004942902641474234 2307 50-51 0.0031282542712816053 2307 52-53 -0.10363170808871303 2307 54-55 -0.03379690405477476 2307 56-57 -0.05217436149425936 2307 58-59 -0.04642910526668942 2307 60-61 -0.16069573205182763 2307 62-63 -0.13379866139182894 2307 64-65 -0.2003920795177052 2307 66-67 -0.27869584293306104 2307 68-69 -0.2860659451222958 2307 70-71 -0.32792461563114017 2307 72-73 -0.42998448484136986 2307 74-75 -0.45815077313839936 2307 76-77 -0.7436020106852332 2307 78-79 -0.35593417825249674 2307 80-81 -0.2235728325635833 2307 82-83 -0.1957487643371465 2307 84-85 -0.40549878951961915 2307 86-87 -0.31933232158772995 2307 88-89 -0.3430150939068781 2307 90-91 -0.34924962095130496 2307 92-93 -0.35049085770999255 2307 94-95 -0.3358088052323467 2307 96-97 -0.4175529547698673 2307 98-99 -0.32991309549937853 2307 100-101 -0.7214726482209031 2201 1 0.10866646546745073 2201 2 0.08123094467805458 2201 3 0.05903212362394328 2201 4 0.03969838651056534 2201 5 0.07014315062830434 2201 6 0.06336574171142217 2201 7 0.08779627469581897 2201 8 0.1269150320711958 2201 9 0.07246146497558215 2201 10-11 0.0725925116365147 2201 12-13 0.07347448312745541 2201 14-15 0.11234253400093763 2201 16-17 0.14725972503456575 2201 18-19 0.10390435357174255 2201 20-21 0.1511592478463868 2201 22-23 0.12194645886270905 2201 24-25 0.09971340024015518 2201 26-27 0.10596052939346379 2201 28-29 0.09058097540032861 2201 30-31 0.0547434026315301 2201 32-33 0.10049288159450498 2201 34-35 0.056849066227172784 2201 36-37 0.05993573699964827 2201 38-39 0.07123969841901356 2201 40-41 0.0736228770710241 2201 42-43 0.08129700291804909 2201 44-45 0.09563185882615244 2201 46-47 0.09715433145036911 2201 48-49 0.07989653995731771 2201 50-51 -0.004109426563481122 2201 52-53 0.06430864280115856 2201 54-55 0.053679542979025996 2201 56-57 -0.04003670966995543 2201 58-59 -0.023453095418162206 2201 60-61 -0.08973778300873647 2201 62-63 -0.10933733570384163 2201 64-65 -0.17367625529993802 2201 66-67 -0.13028619369457317 2201 68-69 -0.24731100097636727 2201 70-71 -0.25461538731203603 2201 72-73 -0.1969055531778281 2201 74-75 -0.3763176126855541 2201 76-77 -0.5589737281468139 2201 78-79 -0.20869463390899057 2201 80-81 -0.13494944573317014 2201 82-83 -0.12545583229827173 2201 84-85 -0.17252159491120977 2201 86-87 -0.12069169805412372 2201 88-89 0.03330184213418619 2201 90-91 -0.09711153427132047 2201 92-93 -0.120557823267049 2201 94-95 -0.12555781220357431 2201 96-97 -0.26465626123565045 2201 98-99 -0.14252398647272457 2201 100-101 -0.40163712683765596 1101 1 0.07478228564016831 1101 2 0.07650693203029135 1101 3 0.06813737777896733 1101 4 0.0524201369833861 1101 5 0.06857685266003699 1101 6 0.07683857682510364 1101 7 0.06417703733984581 1101 8 0.08084724256595877 1101 9 0.0849839023894674 1101 10-11 0.06899946369505727 1101 12-13 0.09561015588678146 1101 14-15 0.09997259473754383 1101 16-17 0.13158733326372385 1101 18-19 0.0900939407069572 1101 20-21 0.09617354566405112 1101 22-23 0.13758239646257664 1101 24-25 0.1374420423704592 1101 26-27 0.10881502230400741 1101 28-29 0.07430727767805934 1101 30-31 0.04640322848334222 1101 32-33 0.06429449620289773 1101 34-35 0.028494651924319214 1101 36-37 0.006512776281091703 1101 38-39 0.004784570605458782 1101 40-41 2.739042944384096E-4 1101 42-43 -0.03756032790450092 1101 44-45 -0.08357967305779823 1101 46-47 -0.01610821975529575 1101 48-49 0.021413569921911346 1101 50-51 -0.023120788256044023 1101 52-53 -0.015403102510262556 1101 54-55 -0.057357689233114684 1101 56-57 -0.05233555976574422 1101 58-59 -0.08618966713979859 1101 60-61 -0.14907900156389786 1101 62-63 -0.14844714783930613 1101 64-65 -0.11377487240748252 1101 66-67 -0.1734584788818836 1101 68-69 -0.17118127695442098 1101 70-71 -0.1815889031838296 1101 72-73 -0.19601197026518946 1101 74-75 -0.41597451267999475 1101 76-77 -0.9627862116710126 1101 78-79 -0.30479894082848347 1101 80-81 -0.03240963278596354 1101 82-83 -0.05905601259370741 1101 84-85 -0.09417509717899719 1101 86-87 0.048219132863629 1101 88-89 -0.16612270353537895 1101 90-91 -0.14878040168014195 1101 92-93 -0.07757059907936537 1101 94-95 -0.02036632754672496 1101 96-97 -0.21838432242061856 1101 98-99 -0.1910894577397073 1101 100-101 -0.34023741390262074 2304 1 0.062296409295200306 2304 2 0.047630957925555606 2304 3 0.027268450741267714 2304 4 0.0038440875478329417 2304 5 0.018970933168397153 2304 6 0.040207548877056354 2304 7 0.03929891427606691 2304 8 0.057460465810507344 2304 9 0.08176314374662752 2304 10-11 0.0813596002536201 2304 12-13 0.0570949015018698 2304 14-15 0.1141340756935918 2304 16-17 0.11223965315824813 2304 18-19 0.11171520300683824 2304 20-21 0.07888886484155933 2304 22-23 0.09566301843798897 2304 24-25 0.049441492708162116 2304 26-27 0.0401717139120521 2304 28-29 0.02971103171242362 2304 30-31 0.019688028785502354 2304 32-33 0.071216060733299 2304 34-35 0.09628349925121427 2304 36-37 0.10560774572654452 2304 38-39 0.06011702068256142 2304 40-41 0.030870950885500292 2304 42-43 0.03251061593880422 2304 44-45 -0.03379484811993905 2304 46-47 0.026487600215091334 2304 48-49 0.03030457389865404 2304 50-51 -0.04862014975167739 2304 52-53 -0.05404080959497293 2304 54-55 0.025265640372062137 2304 56-57 -0.05759149260812535 2304 58-59 -0.04606822778389841 2304 60-61 -0.04579724687659592 2304 62-63 -0.10559400691290222 2304 64-65 -0.08046978289474538 2304 66-67 -0.10431005484799272 2304 68-69 -0.1365269675001315 2304 70-71 -0.12753172406803515 2304 72-73 -0.2499154728478885 2304 74-75 -0.31759995258081375 2304 76-77 -0.8447802840279515 2304 78-79 -0.19911967868799962 2304 80-81 -0.04361024430968996 2304 82-83 -0.040918789918080734 2304 84-85 -0.15875565027214833 2304 86-87 -0.039151391865658525 2304 88-89 -0.10518916256425825 2304 90-91 -0.11840747716826172 2304 92-93 -0.13488907059385724 2304 94-95 -0.10749625986903055 2304 96-97 -0.1952454240985091 2304 98-99 -0.1485885375648195 2304 100-101 -0.19232625624655242 2202 1 0.0726451392156875 2202 2 0.06372666620076473 2202 3 0.05043837527352224 2202 4 0.020742844856272313 2202 5 0.038110003738317744 2202 6 0.020944098163610647 2202 7 0.02384816695004588 2202 8 0.057023184208645716 2202 9 0.05868565623102029 2202 10-11 0.04983208698545383 2202 12-13 0.06985521004306605 2202 14-15 0.042774875447520344 2202 16-17 0.05983266893571937 2202 18-19 0.030679460942117487 2202 20-21 0.042105687531481806 2202 22-23 0.05081426041774506 2202 24-25 0.07498778782900928 2202 26-27 0.016241037046803797 2202 28-29 -0.00960749648163528 2202 30-31 -0.0056408805129919415 2202 32-33 -0.03304766823242744 2202 34-35 -0.06791948757429367 2202 36-37 -0.007668719820784986 2202 38-39 0.06361231253234934 2202 40-41 -0.0042255842245069175 2202 42-43 0.038873906434616856 2202 44-45 0.028244439925998677 2202 46-47 1.023224943992318E-4 2202 48-49 0.036044169449645835 2202 50-51 -0.056144197396470474 2202 52-53 -0.0318458073354293 2202 54-55 0.011163465584367316 2202 56-57 -0.016361573196967072 2202 58-59 -0.010648349401513713 2202 60-61 -0.16064244546381445 2202 62-63 -0.16066183007371393 2202 64-65 -0.049257308470323835 2202 66-67 -0.0409912965222361 2202 68-69 -0.19440873698699335 2202 70-71 -0.20671080374752648 2202 72-73 -0.24806845811055211 2202 74-75 -0.37591621918579676 2202 76-77 -0.6291692465226433 2202 78-79 -0.26961744398293064 2202 80-81 -0.01818162304054738 2202 82-83 -0.009916896978978684 2202 84-85 -0.033750027379802816 2202 86-87 0.062056042643185094 2202 88-89 0.06294977046785277 2202 90-91 -0.13315303437737924 2202 92-93 -0.06949756806172047 2202 94-95 -0.062299127636286755 2202 96-97 -0.10325049327077096 2202 98-99 -0.06423981870383244 2202 100-101 -0.034139911299920556 1102 1 0.036835810773801825 1102 2 0.060640406472757036 1102 3 0.038456547092515336 1102 4 0.012657458148481737 1102 5 0.00961375008189691 1102 6 0.035680175499400946 1102 7 0.043507442970557975 1102 8 0.06534531968546275 1102 9 0.06843562947324955 1102 10-11 0.037552278128323735 1102 12-13 0.0619102879426805 1102 14-15 0.052956301778770865 1102 16-17 0.01839312688174033 1102 18-19 0.03385431889468293 1102 20-21 0.08842593045431357 1102 22-23 0.07740877713028027 1102 24-25 0.05405648618276615 1102 26-27 0.08506436182957344 1102 28-29 0.04312389203090561 1102 30-31 0.02013780106120322 1102 32-33 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50-51 -0.5982487449543257 1213 52-53 -0.27219967377246235 1213 54-55 -0.8280587924201299 1213 56-57 -0.3026574768585206 1213 58-59 -0.15552130672096354 1213 60-61 -0.141318077232512 1213 62-63 -0.01817230104601464 1213 64-65 -0.02059467861570141 1213 66-67 -0.336571007576012 1213 68-69 -0.02208040767957442 1213 70-71 -0.02753376768502136 1213 72-73 0.04197079228353573 1213 74-75 0.12435242974093086 1213 76-77 0.7225895488108272 1213 78-79 -0.003866417579139636 1213 80-81 -0.15235261875205452 1213 82-83 -0.055600215330741776 1213 84-85 -0.04124375171523553 1213 86-87 -1.3016989074064398 1213 88-89 -0.6455229293872833 1213 90-91 -0.33991312380417327 1213 92-93 -0.22514384262025544 1213 94-95 0.09414070914639439 1213 96-97 0.15227347740682973 1213 98-99 0.09726831272534753 1213 100-101 0.5529948837415617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 30.0 8 386.0 9 1177.0 10 832.0 11 316.0 12 252.0 13 434.0 14 1048.0 15 2360.0 16 4326.0 17 6544.0 18 9610.0 19 12979.0 20 17506.0 21 22892.0 22 29929.0 23 38443.0 24 49683.0 25 64600.0 26 82217.0 27 105634.0 28 135434.0 29 173833.0 30 225751.0 31 299023.0 32 409120.0 33 598378.0 34 969488.0 35 1849219.0 36 3957801.0 37 4931619.0 38 660191.0 39 25.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.983692843399034 20.87775815195578 31.143751991853936 27.994797012791246 2 27.744828110230884 21.459514379634463 31.56945715193463 19.22620035820003 3 21.131571326045375 19.616219321329762 39.32075408895469 19.931455263670173 4 21.135712044228296 19.64571434266568 38.15543266428349 21.063140948822532 5 30.12348611105238 18.922143672615704 31.732460128113328 19.221910088218593 6 29.960735503696114 19.552547349506675 31.89367606019801 18.5930410865992 7 30.1800985766262 26.17183370231474 24.42694202242372 19.221125698635337 8 26.684115128746978 21.196873464045833 31.894344496538523 20.224666910668667 9 22.723779868361692 20.289007318832265 35.83235534730656 21.154857465499475 10-11 28.78844341547425 21.244509557282907 28.121168311029955 21.845878716212887 12-13 27.116136678795264 25.929088621710505 24.112854074917905 22.84192062457632 14-15 26.859975273312024 23.40507337725824 25.75867865686857 23.976272692561164 16-17 26.364826562827666 23.035926638030432 25.081915291146505 25.517331507995394 18-19 26.15214211338563 23.941926659983213 25.75556156087252 24.15036966575864 20-21 24.91537852749191 24.4215915596318 26.116684561230546 24.546345351645744 22-23 25.060536122813865 24.452586408873945 25.514186164035245 24.97269130427695 24-25 25.457660628321975 24.2609856489446 25.354738483830868 24.926615238902556 26-27 25.243534197577517 24.314142028704765 25.482140348053132 24.960183425664585 28-29 25.180476119052887 24.91365404774723 24.873019254173688 25.0328505790262 30-31 25.446395426440056 24.726661059714043 24.83897631700834 24.987967196837563 32-33 25.22023954302963 25.13393963692448 24.767165888574937 24.87865493147095 34-35 25.063160413399228 25.218015969080593 24.73515938318877 24.98366423433141 36-37 25.162955403936987 25.091718367062132 24.848817396297214 24.896508832703663 38-39 25.15168801517344 24.583604870660015 25.075339244877714 25.18936786928883 40-41 25.09201668716598 24.960074858212668 24.593396216987724 25.354512237633624 42-43 25.10552943835816 24.853879430357257 24.725353352925662 25.315237778358917 44-45 25.544992984439375 24.62036320485529 24.67054524178012 25.16409856892522 46-47 24.98005481331957 24.73790187858434 24.988040051169648 25.294003256926445 48-49 25.24194898098014 24.51761725151633 24.819708355257173 25.42072541224636 50-51 25.044399618015433 24.693217065811027 25.445166902351605 24.81721641382194 52-53 25.10415486741676 24.55099409415886 25.49514555622896 24.849705482195418 54-55 24.908220900419007 24.699933036686502 24.90352429354864 25.488321769345852 56-57 25.063549331420955 24.86384113391961 25.181569883799167 24.89103965086027 58-59 24.947843654036074 25.19358566316538 25.133232345366842 24.725338337431705 60-61 25.441439595331587 25.040580316863963 24.729345462025552 24.788634625778894 62-63 24.975462145369214 25.282948006282048 25.203564691450225 24.538025156898517 64-65 25.260569915436992 25.185513991866948 24.94123859635156 24.6126774963445 66-67 25.613272969895988 25.023440670899532 25.052443448166184 24.310842911038296 68-69 25.250507859039317 24.780113103793468 25.559115720084552 24.410263317082663 70-71 25.212258787684867 25.222201450073356 25.045573378332154 24.519966383909626 72-73 24.995234229477827 24.76241495585254 25.4855091413601 24.756841673309527 74-75 24.82872614757788 24.76221729601965 25.46671922242942 24.942337333973054 76-77 24.781322059455615 24.766108820614726 25.190673844467682 25.261895275461978 78-79 25.12408620208636 24.774723277059913 24.94978161619703 25.151408904656698 80-81 24.89123273927544 24.619309898080775 25.42904872739858 25.060408635245206 82-83 24.612801888475815 25.45158447966238 25.23274449426467 24.702869137597137 84-85 24.941329636459482 25.048432788005787 25.07270759229721 24.93752998323753 86-87 24.61888542886302 25.272882504586512 25.394978058034678 24.713254008515786 88-89 24.810583863277373 26.26366224132866 24.462849659712006 24.46290423568196 90-91 24.86393011174955 25.263080956518618 24.73900298136134 25.13398595037049 92-93 24.71861146853952 25.57078530505732 24.773552904420836 24.93705032198233 94-95 24.815117472029556 25.77016946435074 24.27288613029251 25.141826933327195 96-97 24.65279030842504 25.024015068385214 24.82878711028171 25.494407512908033 98-99 24.913430774360393 25.155945343310997 24.765497057833237 25.16512682449537 100-101 25.165712164054288 25.81682541285711 24.053147641552624 24.964314781535982 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1800.0 1 1461.0 2 1440.0 3 1740.5 4 1867.0 5 1912.5 6 1832.5 7 1890.5 8 2009.0 9 2208.0 10 2418.0 11 2553.0 12 2731.5 13 2956.0 14 3225.0 15 3464.0 16 3746.0 17 4139.5 18 4528.5 19 5019.0 20 5590.5 21 6269.0 22 7132.5 23 8006.5 24 9174.0 25 10703.5 26 12215.0 27 13961.0 28 16074.0 29 18464.0 30 21417.5 31 25308.5 32 30167.5 33 36381.0 34 43985.0 35 53242.0 36 65079.5 37 79173.5 38 95684.0 39 121983.0 40 165981.5 41 224750.0 42 281143.0 43 342980.5 44 437850.0 45 558943.5 46 701499.0 47 863689.0 48 995999.5 49 1032784.5 50 986932.5 51 933776.0 52 901428.0 53 898586.5 54 866136.0 55 787019.0 56 715411.5 57 597295.0 58 456976.5 59 357154.0 60 272725.5 61 193992.5 62 129917.0 63 82175.5 64 46858.5 65 26006.0 66 16554.0 67 11862.0 68 9146.5 69 7138.0 70 5853.5 71 4813.5 72 3800.0 73 2907.0 74 2220.0 75 1689.0 76 1376.5 77 1180.0 78 903.0 79 579.5 80 356.5 81 209.0 82 134.5 83 89.0 84 60.0 85 42.5 86 30.5 87 22.0 88 14.0 89 11.5 90 11.0 91 7.0 92 3.0 93 1.0 94 1.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15917651916823056 2 0.0 3 0.0 4 0.08184252703859102 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5577921192992443E-4 12-13 5.115584238598488E-5 14-15 0.0 16-17 3.5809089670189414E-4 18-19 0.0 20-21 0.0013982596918835867 22-23 6.820778984797984E-6 24-25 0.0 26-27 6.957194564493944E-4 28-29 6.820778984797984E-6 30-31 8.014415307137632E-4 32-33 0.0 34-35 0.0 36-37 0.0011527116484308592 38-39 0.004965527100932932 40-41 0.0056851192838291206 42-43 0.0016642700722907082 44-45 0.0031205063855450775 46-47 0.01898563830418519 48-49 0.009763945116738315 50-51 0.009054584102319324 52-53 0.012151217761417608 54-55 0.01065746716374685 56-57 0.014968199482139177 58-59 0.022031116120897486 60-61 0.010732495732579627 62-63 0.00389125441082725 64-65 0.013726817706905944 66-67 0.026362310776244208 68-69 0.03039339115625982 70-71 0.013849591728632309 72-73 0.012669596964262257 74-75 0.024790121220248273 76-77 0.041490798564526137 78-79 0.007584706231095358 80-81 0.002083747979855784 82-83 0.002523688224375254 84-85 0.012615030732383872 86-87 0.014129243667009026 88-89 0.01784315782423153 90-91 0.009112560723690107 92-93 0.018733269481747663 94-95 0.026393004281675802 96-97 0.017577147443824406 98-99 0.007762046484700106 100-101 0.3218589187346473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4661082E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.41024772055918 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6544558310997 19.98629206994024 2 9.989679883769925 5.076804809913613 3 2.970975810546418 2.2647969395332077 4 1.8037843714300565 1.8333843085004347 5 0.8918113481632911 1.1330573638417538 6 0.8200306502528361 1.2502309176825475 7 0.55852771068354 0.9934629241085893 8 0.28756109300590327 0.5845598886459807 9 0.28636628315393287 0.6548974374381504 >10 2.7628818226534095 14.39480997405489 >50 0.40585680977382066 7.365033007079546 >100 0.4818120759684135 25.98124661881483 >500 0.06929382978719745 11.867263308008749 >1k 0.01677095344569446 6.290209850273658 >5k 1.915262660110421E-4 0.32395058216401856 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.8416103258954558E-4 0.0 0.0 2 1.8416103258954558E-4 0.0 0.0 3 1.9098181157434357E-4 0.0 0.0 4 1.9098181157434357E-4 0.0 0.0 5 1.9780259055914153E-4 0.0 0.0 6 2.0462336954393953E-4 0.0 0.0 7 2.1826492751353548E-4 0.0 0.0 8 2.3190648548313146E-4 0.0 0.0 9 2.523688224375254E-4 0.0 0.0 10-11 2.864727173615153E-4 0.0 0.0 12-13 3.478597282246972E-4 0.0 0.0 14-15 4.19477907565076E-4 0.0 0.0 16-17 5.013272553826519E-4 0.0 0.0 18-19 6.445636140634094E-4 6.820778984797984E-6 0.0 20-21 8.014415307137632E-4 6.820778984797984E-6 0.0 22-23 9.958337317805058E-4 6.820778984797984E-6 0.0 24-25 0.001210688269801642 6.820778984797984E-6 0.0 26-27 0.0014596467027467685 6.820778984797984E-6 0.0 28-29 0.0017734025360474758 6.820778984797984E-6 0.0 30-31 0.002042823305946996 6.820778984797984E-6 0.0 32-33 0.0023906830341716935 6.820778984797984E-6 0.0 34-35 0.0027692362678279816 6.820778984797984E-6 0.0 36-37 0.0031784830069158604 6.820778984797984E-6 0.0 38-39 0.003710503767730103 6.820778984797984E-6 3.410389492398992E-6 40-41 0.0043448362133163155 6.820778984797984E-6 6.820778984797984E-6 42-43 0.0052042543654008625 6.820778984797984E-6 6.820778984797984E-6 44-45 0.006206908876166166 6.820778984797984E-6 6.820778984797984E-6 46-47 0.007540371167694172 6.820778984797984E-6 6.820778984797984E-6 48-49 0.009190999682015283 6.820778984797984E-6 6.820778984797984E-6 50-51 0.010984864555017154 6.820778984797984E-6 6.820778984797984E-6 52-53 0.013348264473249655 6.820778984797984E-6 6.820778984797984E-6 54-55 0.016212991646864808 6.820778984797984E-6 6.820778984797984E-6 56-57 0.019732513603020568 1.0231168477196975E-5 6.820778984797984E-6 58-59 0.024015962805473703 1.3641557969595968E-5 6.820778984797984E-6 60-61 0.029407788592956507 1.3641557969595968E-5 6.820778984797984E-6 62-63 0.0362046948513077 1.3641557969595968E-5 6.820778984797984E-6 64-65 0.044665871181949604 1.3641557969595968E-5 6.820778984797984E-6 66-67 0.05529946561924966 1.705194746199496E-5 6.820778984797984E-6 68-69 0.06910472228448078 2.0462336954393954E-5 6.820778984797984E-6 70-71 0.08653181259063963 2.0462336954393954E-5 1.3641557969595968E-5 72-73 0.1079661105503673 2.7283115939191935E-5 1.3641557969595968E-5 74-75 0.13510257974138606 2.7283115939191935E-5 1.3641557969595968E-5 76-77 0.16931901751862516 3.069350543159093E-5 1.3641557969595968E-5 78-79 0.21275714848331112 3.410389492398992E-5 1.3641557969595968E-5 80-81 0.26589442716437983 4.092467390878791E-5 1.3641557969595968E-5 82-83 0.3311044846485409 4.774545289358589E-5 1.3641557969595968E-5 84-85 0.4110747078558049 4.774545289358589E-5 1.3641557969595968E-5 86-87 0.5085504603275529 4.774545289358589E-5 1.3641557969595968E-5 88-89 0.6254654329059751 5.456623187838387E-5 1.3641557969595968E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 160055 0.0 73.49955 1 TTTGTGT 172980 0.0 68.09779 2 TAACTTT 17030 0.0 65.06713 1 AACTTTG 17485 0.0 63.70843 2 TTGTGTT 196930 0.0 61.42073 3 TGTGTTT 209535 0.0 58.0479 4 GTGTTTG 234875 0.0 51.80582 5 ACTTTGT 35195 0.0 46.455708 1 TTTGAGG 69715 0.0 38.14137 8 TTGAGGG 33365 0.0 37.485138 9 GTTTGAG 180810 0.0 36.946667 7 TGTTTGA 334985 0.0 36.289673 6 TTGAGTC 20595 0.0 35.95097 4 TTTGAGC 44145 0.0 33.999886 8 TTGAGGC 13080 0.0 33.542755 9 TTGAGGT 20025 0.0 32.674667 9 GTTTGAC 64800 0.0 31.405931 7 TTTGAGT 67670 0.0 31.244328 3 TTTGATG 31040 0.0 30.502676 8 GTTTGAA 26060 0.0 29.943577 2 >>END_MODULE