FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_30429.34100000035b60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_30429.34100000035b60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14661082
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1600550.073.499551
TTTGTGT1729800.068.097792
TAACTTT170300.065.067131
AACTTTG174850.063.708432
TTGTGTT1969300.061.420733
TGTGTTT2095350.058.04794
GTGTTTG2348750.051.805825
ACTTTGT351950.046.4557081
TTTGAGG697150.038.141378
TTGAGGG333650.037.4851389
GTTTGAG1808100.036.9466677
TGTTTGA3349850.036.2896736
TTGAGTC205950.035.950974
TTTGAGC441450.033.9998868
TTGAGGC130800.033.5427559
TTGAGGT200250.032.6746679
GTTTGAC648000.031.4059317
TTTGAGT676700.031.2443283
TTTGATG310400.030.5026768
GTTTGAA260600.029.9435772