FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30427.34200000035d1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30427.34200000035d1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22740931
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA13950.094.98682494-95
TAACTTT245450.062.8901861
AACTTTG253350.061.149822
GTTCTCG16350.052.0995994-95
ACATCCG21000.050.70406394-95
ACTTTGT510100.046.2463071
CTATAAG7950.044.6450994-95
CTTTGTG1007200.043.179391
CAGTCTA8700.040.7963794-95
TTTGTGT1084900.040.121262
TCCGATC18650.035.7218447
CGAAGTA22300.033.95429294-95
CGTCGTA18500.033.2545694-95
TTGTGTT1413000.032.0086483
TCCCTCG13350.031.90367994-95
TGTGTTT1436950.031.5894244
AGATCTA7601.1477823E-931.13407394-95
ATTCGGG10800.030.67282994-95
GGGTGCA12550.030.16656194-95
CGTAGTA16750.029.66565994-95
Produced by FastQC (version 0.11.2)