FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30348.34200000035cf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30348.34200000035cf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16770997
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG174600.10410830077663243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA8000.0106.65272594-95
GTTCTCG12950.098.8287894-95
CTTTGTG1897650.072.4365841
TTTGTGT2022050.068.017042
GACCTAG10600.067.0771994-95
CGTCGTA11250.065.3083494-95
TCTCGCG8400.064.89451694-95
TTGTGTT2280800.061.8410643
TAACTTT172150.060.3851171
AACTTTG178400.058.635262
TGTGTTT2416950.058.6322984
GTGTTTG2657650.053.3030785
ACTTTGT412150.052.2757031
CCCCTGA11250.046.34785594-95
GCTCTAG7200.042.7927694-95
TCCTAGA10300.041.41853394-95
ACATCCG31450.039.1870194-95
TTCGACA11800.038.1619994-95
TTTGAGG898400.037.1637768
TCCTAGG8350.036.89914794-95
Produced by FastQC (version 0.11.2)