FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30304.34200000035cbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30304.34200000035cbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19504740
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG2059700.063.563031
TTTGTGT2172050.060.354582
TAACTTT219200.059.8775441
ACTTTGT625300.058.887251
AACTTTG226050.058.434092
TTGTGTT2453700.054.936623
TGTGTTT2583450.052.419994
GTTCTCG19900.050.0434494-95
AGATCGA15000.045.84137794-95
CGTCGTA13550.045.49723494-95
GTGTTTG3132750.043.257755
CAGTCTA11250.037.93769594-95
TTTGAGG884650.035.628488
GTTTGAG2116450.034.5360457
TTGAGGG406500.033.937429
TGTTTGA3999250.033.8108146
CGTAGTA15600.033.43867594-95
TTGAGGT264350.031.9888239
TTCGACA8905.456968E-1231.96996994-95
TTTGAGA260350.030.9233938
Produced by FastQC (version 0.11.2)