FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30301.34200000035c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30301.34200000035c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14174106
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1120200.062.7163541
TTTGTGT1184900.059.3586582
ACTTTGT346500.057.8629761
TTGTGTT1350900.053.3996053
AGATCGA15000.052.0270494-95
TGTGTTT1436350.050.4800764
TCTCGCG9650.049.01275394-95
GTGTTTG1580450.045.877645
CTCTCGG13600.043.4717994-95
AACTTTG149600.041.8912542
TAACTTT150450.041.3139081
GTTCTCG17400.038.055394-95
GACCTAG10800.037.22473594-95
GCGTCGG9150.036.18373594-95
TTTGAGG470650.033.2715038
CTCGCTG22600.032.4384294-95
TTGAGGG226550.032.431699
GTTTGAG1224350.032.1809547
TGTTTGA2350050.030.84576
TTTGAGT467900.029.2472533
Produced by FastQC (version 0.11.2)