FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30295.34200000035c36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30295.34200000035c36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524697
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA557150.41195007917737453No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366220.27077870949715177No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC232650.1720186411569886No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC165460.12233915480694317No Hit
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT153440.11345170986085677No Hit
CCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA144630.10693770071152056No Hit
TTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACT139240.10295239885965651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA6750.0158.2881394-95
TATGCTA10050.0113.4004694-95
CTATATG7850.0105.8614994-95
TATACTG5100.093.11066494-95
ATATTAG11400.089.55775594-95
ATATTAA23300.086.616994-95
CGTCGTA5500.086.3389894-95
GCGTCGG4800.064.3045694-95
CTATACG5200.063.92405394-95
ATTATGA18100.062.96544394-95
GCGCAAA9750.060.8800594-95
ACTTTGT281750.059.0879251
ATCGGAC4900.058.1466694-95
TATATTG18050.057.87821294-95
TATAGGA11900.057.86162694-95
GTATAGA9500.057.4835894-95
CGTCTAG2952.0654625E-856.3398494-95
CTTTGTG770950.055.868041
TATCGCG3401.0040822E-955.866494-95
TAGCCAG8100.055.69397494-95
Produced by FastQC (version 0.11.2)