FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30292.34200000035c0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30292.34200000035c0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15405556
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA902400.5857626949653748No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554860.36016875989415764No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC235820.1530746439790943No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC172220.11179083702009845No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT168410.10931770330132842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAG9800.0147.5709594-95
TCGTACG1700.0125.5135894-95
TAAATCG3800.0124.7795994-95
CTATACG2650.0116.30398694-95
ATATTAA18050.0107.70447594-95
CGAGGTA3950.0102.0349494-95
TAAGACG2850.099.8236794-95
GTATAGA8300.097.1176194-95
CGCCTAA5550.093.9781494-95
TTGCGCA6400.088.9054694-95
GCTATAA12350.088.3055594-95
TATGCTA11750.084.7439294-95
CTATATG11250.084.2955494-95
CTTATAG3750.082.1881594-95
GCGCAAA9700.080.65649494-95
ATTATGA18150.079.6801894-95
TTGTCTA5100.079.0270794-95
TTAGGCG5450.078.3020594-95
CGACCTA3950.078.02672694-95
GAAATTA12500.077.76263494-95
Produced by FastQC (version 0.11.2)