FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30291.34200000035bbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30291.34200000035bbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12164951
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT215360.17703318328203707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206300.16958555772234513No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC157640.12958539660373478No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124700.10250760566154356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAG10500.090.0646194-95
CTATATG8900.069.066494-95
ACTACCG4600.061.67468394-95
TATGCTA9000.060.41834694-95
ACTTTGT455300.058.8040241
GAGCGTA9950.057.02583394-95
AGATCGA17500.055.38973694-95
ACTAACG8750.054.03876594-95
CTTTGTG1189300.051.0299761
CTTATAA8800.048.3585694-95
TTTGTGT1314000.046.282922
TTGTGTT1355600.045.9694023
ATATTAA26950.045.6171494-95
TGTGTTT1433800.043.5843124
TATATTG16350.043.37974594-95
TTAGGCG7150.042.98538294-95
GCTATAA10650.042.17814694-95
TATACTG7900.041.8971594-95
AGAGCGG10200.041.7211194-95
TCGGAAA19750.040.70008594-95
Produced by FastQC (version 0.11.2)