FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30191.34200000035b87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30191.34200000035b87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16187842
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG13650.084.9673894-95
CTTTGTG1327950.069.510371
AGATCGA12500.068.1681194-95
TTTGTGT1441650.064.096092
GACCTAG8900.061.16832794-95
CTGCCTA9250.058.85384894-95
TTGTGTT1634500.057.9533733
ACATCCG18200.057.22292794-95
TGTGTTT1755700.054.1878474
ACTTTGT348950.049.4976161
GTGTTTG1929750.049.2307785
CTAGTTA28600.046.3458494-95
AACTTTG166550.045.3485372
TAACTTT169850.043.973691
TCCTAGG9200.043.7370994-95
ATTCGGG12550.041.49232594-95
CCGCTAG10950.036.74714794-95
TCCTAGA18400.036.0187894-95
TTTGAGG527500.033.5110248
CGGAGTA14400.032.87428394-95
Produced by FastQC (version 0.11.2)