FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n02_30112.34200000035b51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n02_30112.34200000035b51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12026478
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT214210.1781153218756148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121950.1014012581239495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1596100.062.0585861
TTTGTGT1740500.056.9581032
AGATCGA11850.055.92747594-95
TTGTGTT1882300.053.8851743
TGTGTTT2012200.050.5945324
TAACTTT245350.049.510181
ACTTTGT470500.049.1302761
AACTTTG253100.048.3071332
GTGTTTG2181300.046.612715
TTTGAGG635200.035.8829738
TTGAGGG350250.035.7439089
GTTTGAG1682900.034.807677
TGTTTGA2959500.034.311836
ACGTATA5709.897485E-833.22007894-95
GTTTGAC571900.032.382587
TTTGACC200300.028.9136338
TTGAGGC128050.028.4537589
GTTTGAT715600.028.0478617
GAGCGTA15250.027.9375494-95
TTTGAGA216100.027.5326888
Produced by FastQC (version 0.11.2)