##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l06n01_30427.34100000035d1e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22740931 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.734405596674996 33.0 33.0 33.0 33.0 33.0 2 32.766256931169615 33.0 33.0 33.0 33.0 33.0 3 32.78314370682537 33.0 33.0 33.0 33.0 33.0 4 36.69504898458203 37.0 37.0 37.0 37.0 37.0 5 36.7195352292305 37.0 37.0 37.0 37.0 37.0 6 36.67896710121499 37.0 37.0 37.0 37.0 37.0 7 36.6460905668286 37.0 37.0 37.0 37.0 37.0 8 36.63705676781659 37.0 37.0 37.0 37.0 37.0 9 36.62735448254075 37.0 37.0 37.0 37.0 37.0 10-11 36.61357397812781 37.0 37.0 37.0 37.0 37.0 12-13 36.580321601609015 37.0 37.0 37.0 37.0 37.0 14-15 38.70781666766413 40.0 37.0 40.0 37.0 40.0 16-17 38.661366854329756 40.0 37.0 40.0 37.0 40.0 18-19 38.636083500715074 40.0 37.0 40.0 37.0 40.0 20-21 38.58496923454893 40.0 37.0 40.0 37.0 40.0 22-23 38.52448081391215 40.0 37.0 40.0 37.0 40.0 24-25 38.491396636311855 40.0 37.0 40.0 37.0 40.0 26-27 38.40950111057458 40.0 37.0 40.0 37.0 40.0 28-29 38.35334523023705 40.0 37.0 40.0 37.0 40.0 30-31 38.28585201722832 40.0 37.0 40.0 37.0 40.0 32-33 38.18686365567004 40.0 37.0 40.0 37.0 40.0 34-35 38.0948973241245 40.0 37.0 40.0 37.0 40.0 36-37 38.0173386261099 40.0 37.0 40.0 37.0 40.0 38-39 37.91175669105192 40.0 37.0 40.0 33.0 40.0 40-41 37.75766555028024 40.0 37.0 40.0 33.0 40.0 42-43 37.602977094473395 40.0 37.0 40.0 33.0 40.0 44-45 37.445528835209075 40.0 37.0 40.0 33.0 40.0 46-47 37.22311582142349 37.0 37.0 40.0 33.0 40.0 48-49 36.982847074290845 37.0 37.0 40.0 33.0 40.0 50-51 36.756175835545164 37.0 37.0 40.0 33.0 40.0 52-53 36.476818912998766 37.0 37.0 40.0 33.0 40.0 54-55 36.214841907747754 37.0 37.0 40.0 33.0 40.0 56-57 35.90933757285487 37.0 37.0 38.5 33.0 40.0 58-59 35.604783880659944 37.0 33.0 37.0 33.0 40.0 60-61 35.310544102174184 37.0 33.0 37.0 33.0 40.0 62-63 35.0404950659232 37.0 33.0 37.0 33.0 40.0 64-65 34.77732044039885 37.0 33.0 37.0 27.0 37.0 66-67 34.50930390668702 37.0 33.0 37.0 27.0 37.0 68-69 34.300686348329364 37.0 33.0 37.0 27.0 37.0 70-71 34.091512194465565 37.0 33.0 37.0 27.0 37.0 72-73 33.89535712060337 37.0 33.0 37.0 27.0 37.0 74-75 33.652064728572455 37.0 33.0 37.0 27.0 37.0 76-77 30.67751993091224 33.0 30.0 33.0 24.5 35.0 78-79 33.43953341224244 35.0 33.0 37.0 27.0 37.0 80-81 33.96780362686118 37.0 33.0 37.0 27.0 37.0 82-83 33.97910723180155 37.0 33.0 37.0 27.0 37.0 84-85 33.888614652583925 37.0 33.0 37.0 27.0 37.0 86-87 33.81318689195267 37.0 33.0 37.0 27.0 37.0 88-89 33.65719409200969 37.0 33.0 37.0 27.0 37.0 90-91 33.541495244851674 37.0 33.0 37.0 27.0 37.0 92-93 33.408319474695205 37.0 33.0 37.0 27.0 37.0 94-95 33.296986411857986 37.0 33.0 37.0 27.0 37.0 96-97 33.21467076259982 35.0 33.0 37.0 27.0 37.0 98-99 33.06784579312078 33.0 33.0 37.0 27.0 37.0 100-101 31.033825550062133 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11926821057068082 2306 2 0.08276646877335025 2306 3 0.08662201670264835 2306 4 0.08004149200970545 2306 5 0.074837222305419 2306 6 0.07815030104804777 2306 7 0.08129582095359211 2306 8 0.12433970798780791 2306 9 0.09388091611267413 2306 10-11 0.09320029060142332 2306 12-13 0.13361938206760016 2306 14-15 0.20982335308084288 2306 16-17 0.23325678691112017 2306 18-19 0.19488782055563547 2306 20-21 0.17823062763801545 2306 22-23 0.19919894388591075 2306 24-25 0.21006364998404337 2306 26-27 0.19206694434675597 2306 28-29 0.12900023237700253 2306 30-31 0.1783921263099657 2306 32-33 0.21251516684675664 2306 34-35 0.19255733771687744 2306 36-37 0.18480755515448521 2306 38-39 0.19208222878251746 2306 40-41 0.16177175961950496 2306 42-43 0.12052999684353694 2306 44-45 0.12252488741658141 2306 46-47 0.22269464019959884 2306 48-49 0.1409325117503002 2306 50-51 0.13353011657106606 2306 52-53 0.16795469542240227 2306 54-55 0.1543114742239453 2306 56-57 0.13848607764674625 2306 58-59 0.09835741612432969 2306 60-61 0.07943070924998352 2306 62-63 0.0745036029159678 2306 64-65 0.1558792186835305 2306 66-67 0.1340008748966497 2306 68-69 0.05082631539453786 2306 70-71 0.06595306384456023 2306 72-73 0.0023931279551803186 2306 74-75 -0.07356281511185614 2306 76-77 -0.34095670903706576 2306 78-79 0.12041607226971252 2306 80-81 0.2294397940047901 2306 82-83 0.22881789418215703 2306 84-85 0.22087507757043312 2306 86-87 0.20260697007139328 2306 88-89 0.1683920184795511 2306 90-91 -2.3793561727813994E-4 2306 92-93 0.1433903518082147 2306 94-95 0.10211951509719341 2306 96-97 0.015609026539067372 2306 98-99 0.13538343637824113 2306 100-101 0.17549488875151198 2307 1 0.10811564766540727 2307 2 0.09541589130063954 2307 3 0.06197382525801487 2307 4 0.053243166902703365 2307 5 0.0659894021676024 2307 6 0.09829989644579484 2307 7 0.07685062823893674 2307 8 0.0930350071991981 2307 9 0.1025930157718733 2307 10-11 0.09202728982372577 2307 12-13 0.10833306571387169 2307 14-15 0.18896374538599758 2307 16-17 0.17041366409893044 2307 18-19 0.1613587399581391 2307 20-21 0.15205872087145167 2307 22-23 0.16187549491996123 2307 24-25 0.1389323563625524 2307 26-27 0.13008055566395882 2307 28-29 0.13509961446393248 2307 30-31 0.08929976248540328 2307 32-33 0.08632032017923308 2307 34-35 0.0817814691045129 2307 36-37 0.06242419502112995 2307 38-39 0.030549796261105655 2307 40-41 0.07296550707956584 2307 42-43 0.07471680806887804 2307 44-45 0.04989386134993623 2307 46-47 0.06507268783716569 2307 48-49 -0.04114759703393389 2307 50-51 -0.08498817739215525 2307 52-53 0.03548854267929613 2307 54-55 0.01434049975900109 2307 56-57 0.01275173264912155 2307 58-59 0.012229575643665669 2307 60-61 -0.08832275123064193 2307 62-63 -0.10514142823765127 2307 64-65 -0.14622729186486794 2307 66-67 -0.18307102871130354 2307 68-69 -0.3482675408876261 2307 70-71 -0.33060630733231733 2307 72-73 -0.3814779893306408 2307 74-75 -0.423311199974691 2307 76-77 -0.4299382334809856 2307 78-79 -0.12720318876964853 2307 80-81 -0.0803776837389023 2307 82-83 -0.14413401212341626 2307 84-85 -0.19462124029802652 2307 86-87 -0.12614394870309553 2307 88-89 -0.12082764963959391 2307 90-91 -0.21929249175329346 2307 92-93 -0.1045599111608837 2307 94-95 -0.2468112954817272 2307 96-97 -0.3233818605616179 2307 98-99 -0.20604806429065547 2307 100-101 -0.2086501785722028 2201 1 0.10357998810588498 2201 2 0.06541789193052949 2201 3 0.07832498382422415 2201 4 0.029013321089898625 2201 5 0.050157949413282665 2201 6 0.06256387072782132 2201 7 0.049274810712084616 2201 8 0.095317406641378 2201 9 0.07091584808807738 2201 10-11 0.05638157376897368 2201 12-13 0.08337985611603216 2201 14-15 0.15786100316224605 2201 16-17 0.12500537466104333 2201 18-19 0.1414864498859103 2201 20-21 0.14254256908893126 2201 22-23 0.09147234117357783 2201 24-25 0.1040598203005132 2201 26-27 0.14423613087253528 2201 28-29 0.07999784141386357 2201 30-31 0.0676225581648211 2201 32-33 0.1256089588650866 2201 34-35 0.13353863450635828 2201 36-37 0.10383039707846109 2201 38-39 0.09931507003664564 2201 40-41 0.07166395929336744 2201 42-43 0.07856654532428564 2201 44-45 0.12169035764380709 2201 46-47 0.11514724695604173 2201 48-49 0.03170250763603377 2201 50-51 -0.020057138934397756 2201 52-53 -0.003651020293638396 2201 54-55 0.019448062728791626 2201 56-57 -0.02420558340840273 2201 58-59 -0.04118595034339734 2201 60-61 0.008344829392846975 2201 62-63 -0.05387124088107953 2201 64-65 -0.12197422422146786 2201 66-67 -0.07756147194175611 2201 68-69 -0.2466374495037158 2201 70-71 -0.394283187024854 2201 72-73 -0.3140212210584039 2201 74-75 -0.3071424206088409 2201 76-77 -0.7950953764820596 2201 78-79 -0.18207808855824936 2201 80-81 0.026985170726852914 2201 82-83 -0.040282632755193504 2201 84-85 -0.03541065234704632 2201 86-87 -0.045097368700730556 2201 88-89 -0.12101544584960067 2201 90-91 -0.09657565337134599 2201 92-93 -0.005104624846410388 2201 94-95 -0.2631325819874988 2201 96-97 -0.3601405136784379 2201 98-99 -0.29589924110982935 2201 100-101 -0.39349976430835554 1101 1 0.11041407827042349 1101 2 0.0894339677499687 1101 3 0.09252761977845836 1101 4 0.05935669079649131 1101 5 0.05703702384854381 1101 6 0.0598485013439074 1101 7 0.04058383833272927 1101 8 0.043436490287007246 1101 9 0.0675644145867409 1101 10-11 0.06885308823095215 1101 12-13 0.09083176715621022 1101 14-15 0.08323557547343086 1101 16-17 0.05865643414460919 1101 18-19 0.07111707985832538 1101 20-21 0.07610777837204807 1101 22-23 0.07589079979628366 1101 24-25 0.022779352365823513 1101 26-27 0.04869854797860995 1101 28-29 0.04438207988674492 1101 30-31 0.031408086881896224 1101 32-33 0.04867083792355942 1101 34-35 0.0811711634612351 1101 36-37 -0.07298557974412745 1101 38-39 -0.10680518167493602 1101 40-41 -0.05943549806001869 1101 42-43 -0.08436746684218832 1101 44-45 -0.14532566895730525 1101 46-47 -0.07454326167675873 1101 48-49 -0.1047929199551092 1101 50-51 -0.15672073310505397 1101 52-53 -0.11082681229601121 1101 54-55 -0.06505805573137735 1101 56-57 -0.06466804479554611 1101 58-59 -0.10422208015725687 1101 60-61 -0.08197555279061675 1101 62-63 -0.16967279869162866 1101 64-65 -0.2696326005134253 1101 66-67 -0.15388287577049908 1101 68-69 -0.34408118189848125 1101 70-71 -0.34513178572491654 1101 72-73 -0.35135474320640014 1101 74-75 -0.4293496083149506 1101 76-77 -0.794072103748988 1101 78-79 -0.1900827741265303 1101 80-81 -0.04022815704589533 1101 82-83 -0.04236316788647798 1101 84-85 -0.05313813273926371 1101 86-87 -0.16836228018981103 1101 88-89 -0.1212022399109145 1101 90-91 -0.20214224813796022 1101 92-93 -0.1474625775984535 1101 94-95 -0.16450393790996998 1101 96-97 -0.23630825324413962 1101 98-99 -0.2763326870637641 1101 100-101 -0.4175250055134754 2304 1 0.08489772363852666 2304 2 0.07293622720214898 2304 3 0.0573419752043236 2304 4 0.03689427398946776 2304 5 0.04180845976567582 2304 6 0.03833392092059995 2304 7 0.03292333495617328 2304 8 0.06652210118519264 2304 9 0.06100750143647815 2304 10-11 0.05683114044219906 2304 12-13 0.058355145671328046 2304 14-15 0.0980561954773762 2304 16-17 0.08962178115690023 2304 18-19 0.09168326986329589 2304 20-21 0.0741102993368088 2304 22-23 0.06355884007837886 2304 24-25 0.06487262637946145 2304 26-27 0.10141903092346638 2304 28-29 0.061782624664182606 2304 30-31 0.061220949768731714 2304 32-33 0.07389167763646753 2304 34-35 0.051037268442925665 2304 36-37 -0.010185869353286137 2304 38-39 -0.059431244197178046 2304 40-41 -0.02179166097976548 2304 42-43 0.03270496928312383 2304 44-45 0.011812109489014233 2304 46-47 0.03339711249574151 2304 48-49 0.009828249784270326 2304 50-51 -0.08765256500950613 2304 52-53 -0.04753273392419288 2304 54-55 -0.013168608223445233 2304 56-57 -0.017704370643365053 2304 58-59 -0.08648268752954635 2304 60-61 -0.08100167815440074 2304 62-63 -0.10068397165353105 2304 64-65 -0.10857388329720408 2304 66-67 -0.06968742194600708 2304 68-69 -0.177433179187247 2304 70-71 -0.23358681967827266 2304 72-73 -0.27803713230333216 2304 74-75 -0.30858332575017755 2304 76-77 -0.7235475559761042 2304 78-79 -0.18823824164181246 2304 80-81 0.030665732477935137 2304 82-83 0.02203790465635791 2304 84-85 0.021073838520813126 2304 86-87 -0.07509424133528597 2304 88-89 -0.10066292539686117 2304 90-91 -0.09754306980622118 2304 92-93 -0.03941977163519539 2304 94-95 -0.11597290943326044 2304 96-97 -0.24083375102005533 2304 98-99 -0.1688697931270724 2304 100-101 -0.2689872453098765 2202 1 0.07806158498042493 2202 2 0.0688725916047872 2202 3 0.053864878968987284 2202 4 0.025508447913608734 2202 5 0.022243345566657524 2202 6 0.047163718973678215 2202 7 0.05734417092253352 2202 8 0.060463468253090014 2202 9 0.024040574413795923 2202 10-11 0.05649020730277243 2202 12-13 0.06193029377802617 2202 14-15 0.08136062758985219 2202 16-17 0.05892379673319681 2202 18-19 0.05305788514039733 2202 20-21 0.036761913547451286 2202 22-23 0.02304940887123763 2202 24-25 0.03068800967096763 2202 26-27 0.0676158277809904 2202 28-29 0.015570319483380501 2202 30-31 -0.002862435111019579 2202 32-33 0.054668899089016065 2202 34-35 0.061796023072268724 2202 36-37 -0.06407299350191664 2202 38-39 -0.05317832975497794 2202 40-41 0.02064741643669521 2202 42-43 -0.026185186900470114 2202 44-45 -0.012046583852871606 2202 46-47 0.037798048390286 2202 48-49 -0.0377174505574871 2202 50-51 -0.038434997866588105 2202 52-53 -0.038708863615831035 2202 54-55 -0.004077873172725788 2202 56-57 -0.004287008304423523 2202 58-59 -0.056053310011087376 2202 60-61 -0.08192064130729193 2202 62-63 -0.07826349123821075 2202 64-65 -0.07857371711371997 2202 66-67 -0.058090384772803816 2202 68-69 -0.17851182467624227 2202 70-71 -0.23344460933741118 2202 72-73 -0.17533430723423038 2202 74-75 -0.15337767539395486 2202 76-77 -0.22301887476023907 2202 78-79 -0.0376064132770253 2202 80-81 0.09005838577629532 2202 82-83 -0.02240903549632378 2202 84-85 -0.024076136029137274 2202 86-87 0.008505863325702023 2202 88-89 -0.06479173746427591 2202 90-91 -0.07057046888577645 2202 92-93 -0.013089186793472152 2202 94-95 -0.09526144560219052 2202 96-97 -0.20802919593334934 2202 98-99 -0.08755386030134105 2202 100-101 0.027593901194055803 1102 1 0.0757907316860269 1102 2 0.06343555331516626 1102 3 0.06175752950542801 1102 4 -0.02993656756155616 1102 5 0.0285377841122596 1102 6 0.02424689124314483 1102 7 0.03163011871206578 1102 8 0.05968366337900477 1102 9 0.04740378672265422 1102 10-11 0.023940470781532497 1102 12-13 0.04793251928249731 1102 14-15 0.08502429120569133 1102 16-17 0.06223286769559877 1102 18-19 0.04656788248905741 1102 20-21 0.013720808456781697 1102 22-23 0.030099983744960923 1102 24-25 0.03498037130106013 1102 26-27 0.006776854678825828 1102 28-29 0.016047152974088874 1102 30-31 0.019382542760858712 1102 32-33 9.193914497913624E-4 1102 34-35 0.030550299408353965 1102 36-37 -0.06917979175305788 1102 38-39 -0.08056662454541907 1102 40-41 -0.06585248675608568 1102 42-43 -0.09822311506371761 1102 44-45 -0.06868862837898604 1102 46-47 -0.06237665342077747 1102 48-49 -0.1008741519676235 1102 50-51 -0.16514325448044076 1102 52-53 -0.08184497925442002 1102 54-55 -0.05363324400953928 1102 56-57 -0.05820669710828241 1102 58-59 -0.09662287599137898 1102 60-61 -0.18355133149454161 1102 62-63 -0.252485178768616 1102 64-65 -0.2131131002808644 1102 66-67 -0.150533215184403 1102 68-69 -0.21140410965200118 1102 70-71 -0.2792003424629641 1102 72-73 -0.3266901307775285 1102 74-75 -0.36125449582581837 1102 76-77 -0.5423215476144598 1102 78-79 -0.23252792124595345 1102 80-81 -0.12939849094853884 1102 82-83 -0.18633466423897005 1102 84-85 -0.22517658346576042 1102 86-87 -0.27009510120434754 1102 88-89 -0.2777532765234696 1102 90-91 -0.39803888540816956 1102 92-93 -0.3124653255210177 1102 94-95 -0.40501269350329494 1102 96-97 -0.42186383988013887 1102 98-99 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36-37 -0.3731755284923324 1213 38-39 -0.40949711078171447 1213 40-41 -0.3231741389298648 1213 42-43 -0.2850139714430213 1213 44-45 -0.2956908951552961 1213 46-47 -0.38666212805537015 1213 48-49 -0.28555607880816325 1213 50-51 -0.23431478853037646 1213 52-53 -0.21886546888169534 1213 54-55 -0.718501721564671 1213 56-57 -1.640799434010951 1213 58-59 -0.5592246052543786 1213 60-61 -0.37362017188180374 1213 62-63 -0.27685034300680655 1213 64-65 -1.6652746818291178 1213 66-67 -0.6766582995945853 1213 68-69 -0.3007615595891693 1213 70-71 -0.1695176087809358 1213 72-73 -1.0547979636842442 1213 74-75 -0.45126660797319573 1213 76-77 -0.4482772087616631 1213 78-79 -0.49171791291531974 1213 80-81 -0.3965978634948115 1213 82-83 -1.3520256214286377 1213 84-85 -0.6629206474097415 1213 86-87 -0.46666867237212983 1213 88-89 -1.2509672376601628 1213 90-91 -1.5218800397248984 1213 92-93 -2.7055455560445942 1213 94-95 -1.2539124474838772 1213 96-97 -0.46263529631641376 1213 98-99 -0.16134593353492477 1213 100-101 0.055463615481297524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 62.0 8 657.0 9 1798.0 10 1178.0 11 385.0 12 312.0 13 568.0 14 1291.0 15 2905.0 16 5404.0 17 8783.0 18 12861.0 19 17775.0 20 23672.0 21 31646.0 22 41317.0 23 54053.0 24 70097.0 25 91135.0 26 119054.0 27 154021.0 28 199225.0 29 258324.0 30 336884.0 31 449404.0 32 619783.0 33 914463.0 34 1506657.0 35 2914828.0 36 6265652.0 37 7664478.0 38 972208.0 39 46.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.647320660801423 24.59746768099184 27.450423488173755 24.304788170032985 2 25.9946745364119 24.423151365262928 26.76582150484516 22.816352593480012 3 24.121030928768924 23.152121608389738 28.72264992141263 24.004197541428713 4 24.403064177459353 22.73125608290057 28.108708550167517 24.75697118947257 5 26.80297917442342 22.141270293639252 27.424374138420276 23.631376393517044 6 27.23483484471238 22.399980018408215 27.102887740172115 23.262297396707286 7 26.460187579831274 23.91280726369558 26.324617052837457 23.30238810363569 8 26.709201131651117 23.114339514068266 26.53352670565686 23.642932648623752 9 24.778114844990295 22.774366625535254 28.712206197714597 23.735312331759857 10-11 26.26719564097496 23.31354809942366 26.62613097610843 23.793125283492955 12-13 26.217219867323134 24.48551319859322 25.215051504471713 24.082215429611935 14-15 26.27239710720797 23.879893987245755 25.279127782570953 24.56858112297532 16-17 26.091403205963733 23.556023278026743 25.114191674914277 25.238381841095247 18-19 26.033613135715505 23.726077881332124 25.260845741100045 24.979463241852322 20-21 25.550818352916345 23.98800613874622 25.380804545134733 25.080370963202704 22-23 25.598912375223335 24.13546745293761 25.12781248929518 25.137807682543865 24-25 25.754504070215944 24.087452708070746 24.946817260911615 25.21122596080169 26-27 25.618909357756724 24.167277935982483 25.0915123043995 25.122300401861295 28-29 25.69649285224011 24.286660849541537 24.83443158210838 25.182414716109975 30-31 25.803950791245718 24.175216711835624 24.802799254365908 25.21803324255275 32-33 25.76008651536738 24.493742142746928 24.642150754513963 25.10402058737173 34-35 25.62880561046511 24.459574676164316 24.736726917644663 25.17489279572591 36-37 25.736327521174378 24.361080231029355 24.688393584616936 25.214198663179328 38-39 25.724160682656883 24.32960943479878 24.677179210183706 25.26905067236063 40-41 25.631134122942157 24.459781186301484 24.66296781631693 25.24611687443943 42-43 25.58028133352101 24.370298918130317 24.684624524024084 25.364795224324588 44-45 25.79651460329541 24.24017051975157 24.62905700962665 25.334257867326365 46-47 25.68952380994264 24.225332230095827 24.640271056924547 25.44487290303699 48-49 25.736254784625977 24.247885512160014 24.597194760849394 25.41866494236461 50-51 25.521387222535107 24.463133288645107 24.827761003201044 25.187718485618742 52-53 25.587505200537702 24.403356892393933 24.868803092922093 25.14033481414627 54-55 25.38447863292968 24.34497570543298 24.82746981938079 25.44307584225655 56-57 25.542335404523804 24.499509947921947 24.775892239251142 25.182262408303107 58-59 25.330707999431485 24.480411034116887 24.90405312237185 25.28482784407978 60-61 25.619914471413974 24.536231633254104 24.493596856377316 25.350257038954606 62-63 25.44335256889751 24.594784624466286 24.722941338074563 25.238921468561642 64-65 25.521999565804336 24.659549678011132 24.71137125811613 25.10707949806841 66-67 25.66636139825643 24.61674255932716 24.602012440374963 25.11488360204145 68-69 25.595785014023377 24.603232582342816 24.775748951595094 25.025233452038716 70-71 25.48647401720118 24.77414108832235 24.63877757942436 25.100607315052116 72-73 25.49135222675804 24.624654018150434 24.66720815009155 25.216785604999973 74-75 25.360816104371636 24.635554199413377 24.682857904705354 25.32077179150963 76-77 25.458757078127064 24.610755385783563 24.556829312276285 25.373658223813088 78-79 25.332579883780827 24.545485866895405 24.603252238125325 25.518682011198447 80-81 25.348499577378718 24.548901832188516 24.590703334461335 25.511895255971428 82-83 25.192052172871808 24.803624068382803 24.615904998764215 25.388418759981167 84-85 25.38829233829229 24.725142490306503 24.484492439198988 25.402072732202218 86-87 25.245871717803446 24.813839168300564 24.577203473096347 25.36308564079965 88-89 25.268917889507176 25.303742674832037 24.304669811071804 25.12266962458899 90-91 25.403026358163533 24.954044461263507 24.30749575147125 25.335433429101712 92-93 25.239990020231133 25.077912240270084 24.2621354088379 25.419962330660884 94-95 25.385409478065135 25.123845368279333 24.04559307724834 25.445152076407183 96-97 25.283181731919484 24.985016755216503 24.20444308987442 25.5273584229896 98-99 25.418404318310834 24.980613749982076 24.19522321040171 25.405758721305382 100-101 25.59792531328971 25.14348222704635 23.87077517937649 25.38781728028745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3874.0 1 2536.5 2 1221.5 3 1260.0 4 1371.0 5 1348.0 6 1224.0 7 1297.0 8 1422.0 9 1542.0 10 1592.0 11 1703.0 12 1849.0 13 1920.0 14 2067.0 15 2213.5 16 2371.5 17 2575.0 18 2725.0 19 2872.0 20 3180.5 21 3566.5 22 4030.5 23 4661.5 24 5418.5 25 6397.0 26 7434.0 27 8479.5 28 10011.0 29 12154.0 30 14904.5 31 18508.0 32 23877.5 33 30459.5 34 38307.5 35 50187.0 36 65046.0 37 85252.5 38 112068.5 39 146079.5 40 198531.5 41 289015.5 42 379019.0 43 433041.0 44 545566.5 45 706211.5 46 897143.5 47 1193570.0 48 1555475.0 49 1741545.5 50 1670521.0 51 1544876.0 52 1465248.5 53 1469270.0 54 1422010.5 55 1292467.0 56 1204331.0 57 1034420.0 58 800773.0 59 636530.0 60 513952.5 61 383579.0 62 255010.0 63 159219.5 64 90315.0 65 49597.0 66 30171.5 67 20738.5 68 16013.5 69 12799.5 70 10312.5 71 8142.0 72 6346.5 73 4886.5 74 3764.5 75 2845.0 76 2067.0 77 1446.5 78 971.5 79 668.5 80 449.5 81 297.0 82 208.5 83 130.0 84 89.5 85 71.5 86 45.5 87 37.0 88 31.5 89 22.5 90 17.0 91 11.5 92 11.5 93 8.0 94 4.5 95 4.5 96 3.5 97 2.5 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1533182612444495 2 0.0 3 0.0 4 0.0990900504469232 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.9022558487161324E-4 12-13 1.8249032988139314E-4 14-15 0.00119168384091223 16-17 0.0 18-19 0.0 20-21 5.760538123966868E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.3192072039618782E-4 30-31 7.91524322377127E-5 32-33 0.0 34-35 0.0 36-37 8.113124304365551E-4 38-39 0.0026604011946564543 40-41 0.0011499089461201038 42-43 9.674186162387106E-5 44-45 4.6172252138665734E-4 46-47 0.00789765379438511 48-49 0.0039971978280044914 50-51 0.005109729236679008 52-53 0.00217229452919056 54-55 0.007886660401018762 56-57 0.013374562369500177 58-59 0.019882651242378774 60-61 0.010780121535041815 62-63 0.008680383402069159 64-65 0.010265630725496683 66-67 0.022872854238025698 68-69 0.018679974008100196 70-71 0.012741342911598473 72-73 0.017626806923603962 74-75 0.021408534241628016 76-77 0.03499197108508882 78-79 8.420939318623323E-4 80-81 5.342789176045607E-4 82-83 0.001321405882635148 84-85 0.009249841178446037 86-87 0.017022170288454767 88-89 0.018693166080139816 90-91 0.02121724919705354 92-93 0.02169436246915309 94-95 0.029611804371597626 96-97 0.028008967618783947 98-99 0.02526281795586997 100-101 0.302472225081726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2740931E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.33163539592201 #Duplication Level Percentage of deduplicated Percentage of total 1 80.95570240207655 15.650061220577117 2 8.138419096614617 3.146579013499255 3 2.8313023684968175 1.6420111525017271 4 1.7256588005135267 1.3343922699716646 5 0.9559344535245201 0.9239888158967989 6 0.6688901441839905 0.7758444232374365 7 0.4300840133089072 0.5819959134431866 8 0.3394947259301535 0.5250390608416158 9 0.24277749164425969 0.42239573557229976 >10 2.4066812931256134 10.1273367566461 >50 0.5499085984179837 7.39598354917963 >100 0.5934233649304566 24.953952079685656 >500 0.10942741916680696 14.355943276057282 >1k 0.05195917306945549 17.804743767291605 >5k 3.3665499655084877E-4 0.3597329655986047 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1433129101002944E-4 0.0 0.0 2 1.1433129101002944E-4 0.0 0.0 3 1.2312600570310863E-4 0.0 0.0 4 1.3192072039618782E-4 0.0 0.0 5 1.4071543508926702E-4 0.0 0.0 6 1.583048644754254E-4 0.0 0.0 7 1.7589429386158376E-4 0.0 0.0 8 1.8908636590120255E-4 0.0 0.0 9 2.1107315263390052E-4 0.0 0.0 10-11 2.506493687527569E-4 0.0 0.0 12-13 3.495899090498977E-4 0.0 0.0 14-15 4.507291280203084E-4 0.0 0.0 16-17 6.17828707188813E-4 0.0 0.0 18-19 8.13511109109825E-4 4.397357346539594E-6 0.0 20-21 0.0010157895470506461 4.397357346539594E-6 4.397357346539594E-6 22-23 0.001277432309169752 4.397357346539594E-6 4.397357346539594E-6 24-25 0.0016336182542394592 4.397357346539594E-6 4.397357346539594E-6 26-27 0.0020205857007349433 4.397357346539594E-6 4.397357346539594E-6 28-29 0.002438334648656205 4.397357346539594E-6 4.397357346539594E-6 30-31 0.0029352360288151793 6.596036019809391E-6 4.397357346539594E-6 32-33 0.0035794488800832296 8.794714693079189E-6 6.596036019809391E-6 34-35 0.004307211520935533 8.794714693079189E-6 8.794714693079189E-6 36-37 0.005193279026263261 8.794714693079189E-6 8.794714693079189E-6 38-39 0.006402552296561649 8.794714693079189E-6 8.794714693079189E-6 40-41 0.007792117218068161 8.794714693079189E-6 8.794714693079189E-6 42-43 0.00954446412066419 8.794714693079189E-6 8.794714693079189E-6 44-45 0.011648599610983384 8.794714693079189E-6 8.794714693079189E-6 46-47 0.014064947472906892 1.0993393366348986E-5 8.794714693079189E-6 48-49 0.016830885243880295 1.3192072039618782E-5 8.794714693079189E-6 50-51 0.020225645115408863 1.3192072039618782E-5 8.794714693079189E-6 52-53 0.024189862763314307 1.3192072039618782E-5 8.794714693079189E-6 54-55 0.029103909598072304 1.3192072039618782E-5 1.3192072039618782E-5 56-57 0.03511949444813847 1.3192072039618782E-5 1.3192072039618782E-5 58-59 0.042584008543889426 1.7589429386158378E-5 1.3192072039618782E-5 60-61 0.05129737212605764 1.7589429386158378E-5 1.3192072039618782E-5 62-63 0.06191699011795075 1.7589429386158378E-5 1.3192072039618782E-5 64-65 0.07502551236798528 1.9788108059428173E-5 1.3192072039618782E-5 66-67 0.09076585298992376 2.198678673269797E-5 1.3192072039618782E-5 68-69 0.11073645137923332 2.198678673269797E-5 1.3192072039618782E-5 70-71 0.1352495199075183 2.4185465405967765E-5 1.3192072039618782E-5 72-73 0.16501083442889827 2.6384144079237565E-5 1.3192072039618782E-5 74-75 0.20164301980424637 2.6384144079237565E-5 1.3192072039618782E-5 76-77 0.24682146918259418 2.6384144079237565E-5 1.7589429386158378E-5 78-79 0.30194674087881446 2.6384144079237565E-5 1.9788108059428173E-5 80-81 0.36875359236611727 2.6384144079237565E-5 2.198678673269797E-5 82-83 0.4490735229793362 2.6384144079237565E-5 2.198678673269797E-5 84-85 0.5474006319266349 2.6384144079237565E-5 2.6384144079237565E-5 86-87 0.6663513468292042 2.6384144079237565E-5 2.6384144079237565E-5 88-89 0.8090807715831863 2.858282275250736E-5 2.6384144079237565E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACTTT 23835 0.0 65.08612 1 AACTTTG 24850 0.0 62.866478 2 ACTTTGT 49060 0.0 46.262375 1 CTTTGTG 93295 0.0 41.751625 1 TTTGTGT 99650 0.0 39.1429 2 TCCGATC 1730 0.0 34.035313 7 TTGTGTT 132495 0.0 30.784695 3 TGTGTTT 135555 0.0 30.23352 4 GTGTTTG 152330 0.0 26.992027 5 TGTTTGA 172945 0.0 23.689524 6 GTTTGAG 97540 0.0 23.513569 7 TTTGAGG 38230 0.0 23.127546 8 TTGAGGG 21810 0.0 19.225021 9 GTTTGAC 36690 0.0 18.442566 7 TTGAGGT 12750 0.0 17.318272 9 TTTGAGC 32360 0.0 17.036402 8 TTTGACC 11170 0.0 16.324211 8 TTTGATG 19420 0.0 16.260227 8 TTGAGGC 8860 0.0 15.649876 9 AACTTGT 5075 0.0 15.545972 2 >>END_MODULE