##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l06n01_30348.34100000035cf3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16770997 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74260015668717 33.0 33.0 33.0 33.0 33.0 2 32.77632289839418 33.0 33.0 33.0 33.0 33.0 3 32.792163876721226 33.0 33.0 33.0 33.0 33.0 4 36.715417157369956 37.0 37.0 37.0 37.0 37.0 5 36.739449837120596 37.0 37.0 37.0 37.0 37.0 6 36.68711007461274 37.0 37.0 37.0 37.0 37.0 7 36.65448822154103 37.0 37.0 37.0 37.0 37.0 8 36.64673286865414 37.0 37.0 37.0 37.0 37.0 9 36.63803100078069 37.0 37.0 37.0 37.0 37.0 10-11 36.63037582082926 37.0 37.0 37.0 37.0 37.0 12-13 36.59100705223428 37.0 37.0 37.0 37.0 37.0 14-15 38.71894053168097 40.0 37.0 40.0 37.0 40.0 16-17 38.68494940998439 40.0 37.0 40.0 37.0 40.0 18-19 38.6577092584299 40.0 37.0 40.0 37.0 40.0 20-21 38.602933713481676 40.0 37.0 40.0 37.0 40.0 22-23 38.53890877805297 40.0 37.0 40.0 37.0 40.0 24-25 38.50613624819085 40.0 37.0 40.0 37.0 40.0 26-27 38.42956179051251 40.0 37.0 40.0 37.0 40.0 28-29 38.37852305381725 40.0 37.0 40.0 37.0 40.0 30-31 38.32000491682158 40.0 37.0 40.0 37.0 40.0 32-33 38.22471168529813 40.0 37.0 40.0 37.0 40.0 34-35 38.12957691185562 40.0 37.0 40.0 37.0 40.0 36-37 38.042867576686106 40.0 37.0 40.0 37.0 40.0 38-39 37.952066743557346 40.0 37.0 40.0 33.0 40.0 40-41 37.81849284213693 40.0 37.0 40.0 33.0 40.0 42-43 37.67734679101069 40.0 37.0 40.0 33.0 40.0 44-45 37.54696250318332 40.0 37.0 40.0 33.0 40.0 46-47 37.36169259943222 38.5 37.0 40.0 33.0 40.0 48-49 37.154665014846756 37.0 37.0 40.0 33.0 40.0 50-51 36.94827701656616 37.0 37.0 40.0 33.0 40.0 52-53 36.686834032586134 37.0 37.0 40.0 33.0 40.0 54-55 36.44355788746489 37.0 37.0 40.0 33.0 40.0 56-57 36.16889356667347 37.0 37.0 40.0 33.0 40.0 58-59 35.88687688036674 37.0 37.0 38.5 33.0 40.0 60-61 35.59946394361647 37.0 33.0 37.0 33.0 40.0 62-63 35.334879435015104 37.0 33.0 37.0 33.0 40.0 64-65 35.06213518492669 37.0 33.0 37.0 33.0 40.0 66-67 34.782225439549 37.0 33.0 37.0 30.0 37.0 68-69 34.55345004235586 37.0 33.0 37.0 27.0 37.0 70-71 34.33604749318123 37.0 33.0 37.0 27.0 37.0 72-73 34.12950163308717 37.0 33.0 37.0 27.0 37.0 74-75 33.8753812012488 37.0 33.0 37.0 27.0 37.0 76-77 30.93787626340879 33.0 30.0 33.0 24.5 37.0 78-79 33.654174942610744 35.0 33.0 37.0 27.0 37.0 80-81 34.13452485859964 37.0 33.0 37.0 27.0 37.0 82-83 34.132139013560135 37.0 33.0 37.0 27.0 37.0 84-85 34.03519039446492 37.0 33.0 37.0 27.0 37.0 86-87 33.94841666240832 37.0 33.0 37.0 27.0 37.0 88-89 33.79431526939037 37.0 33.0 37.0 27.0 37.0 90-91 33.67233170454922 37.0 33.0 37.0 27.0 37.0 92-93 33.54510056855892 37.0 33.0 37.0 27.0 37.0 94-95 33.42499593196517 37.0 33.0 37.0 27.0 37.0 96-97 33.37018294141964 37.0 33.0 37.0 27.0 37.0 98-99 33.22040439814043 35.0 33.0 37.0 27.0 37.0 100-101 31.17645343326935 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10425439434441586 2306 2 0.06971870934286528 2306 3 0.07885284754774347 2306 4 0.035329715908979153 2306 5 0.04155620114960357 2306 6 0.05153701989996762 2306 7 0.11415069748223772 2306 8 0.10642907447751071 2306 9 0.07600486445862487 2306 10-11 0.08109034328312958 2306 12-13 0.1074554715278424 2306 14-15 0.21138724616576354 2306 16-17 0.20601560449642164 2306 18-19 0.17876226871128154 2306 20-21 0.19691331537552514 2306 22-23 0.1905285177046494 2306 24-25 0.20040010908243744 2306 26-27 0.20224317513302736 2306 28-29 0.17379385433373074 2306 30-31 0.18838366443815602 2306 32-33 0.1809751360636298 2306 34-35 0.13736402915936452 2306 36-37 0.15376482600817099 2306 38-39 0.17316271125537241 2306 40-41 0.1836735415731141 2306 42-43 0.15174397090314784 2306 44-45 0.13897621583028297 2306 46-47 0.20238053771479514 2306 48-49 0.16243541952137974 2306 50-51 0.1390465597362791 2306 52-53 0.17222518538712706 2306 54-55 0.14803171164688678 2306 56-57 0.14779550226386107 2306 58-59 0.12124103948068665 2306 60-61 0.09624928204555516 2306 62-63 0.1413204160498296 2306 64-65 0.12807573975326392 2306 66-67 0.1711390818096561 2306 68-69 0.055482377317311204 2306 70-71 0.04125811201499374 2306 72-73 -0.006244608356198 2306 74-75 -0.02260902611713078 2306 76-77 -0.283581469070171 2306 78-79 0.1094725039055433 2306 80-81 0.1981468276286904 2306 82-83 0.17944315562847635 2306 84-85 0.10782714116168535 2306 86-87 0.10724906000790924 2306 88-89 0.19040300814607036 2306 90-91 0.009830996819673032 2306 92-93 0.15197366486658126 2306 94-95 0.14206130313948506 2306 96-97 0.09959713494149014 2306 98-99 0.19584435829657565 2306 100-101 0.17892651326210185 2307 1 0.11646465979050191 2307 2 0.07721473817772306 2307 3 0.07328876894503367 2307 4 0.03124490818263581 2307 5 0.05580661710990853 2307 6 0.0883517936147129 2307 7 0.07770479435526312 2307 8 0.1024959790697153 2307 9 0.10349893696491108 2307 10-11 0.08657426327959428 2307 12-13 0.11153554195039561 2307 14-15 0.21653121025829591 2307 16-17 0.18766811257658134 2307 18-19 0.15400833441673 2307 20-21 0.15657951230019762 2307 22-23 0.14053130671813108 2307 24-25 0.1711477325767632 2307 26-27 0.1619290888610223 2307 28-29 0.1350766479403589 2307 30-31 0.09944330270475632 2307 32-33 0.08205314017838106 2307 34-35 0.08596754118423888 2307 36-37 0.038065817002049585 2307 38-39 0.05835677296140318 2307 40-41 0.06310452826797786 2307 42-43 0.06209764877977619 2307 44-45 0.005252859639114149 2307 46-47 0.06235037406948152 2307 48-49 -0.0666821171101688 2307 50-51 -0.06652346718056634 2307 52-53 0.00926743994946122 2307 54-55 0.011209325302381501 2307 56-57 -0.03562320839675692 2307 58-59 -0.09820305458922718 2307 60-61 -0.112089412325588 2307 62-63 -0.09999699887978863 2307 64-65 -0.10334376413888435 2307 66-67 -0.1429657836213991 2307 68-69 -0.31238667261596476 2307 70-71 -0.3113342302250146 2307 72-73 -0.3255789234820128 2307 74-75 -0.36356689945540666 2307 76-77 -0.45110451265989937 2307 78-79 -0.11957135743954694 2307 80-81 -0.06241261592747094 2307 82-83 -0.16210613615579916 2307 84-85 -0.24206138329377325 2307 86-87 -0.14634203698667392 2307 88-89 -0.12437695272419802 2307 90-91 -0.23702796322314867 2307 92-93 -0.1834066624851971 2307 94-95 -0.30825654293089144 2307 96-97 -0.3746787619151135 2307 98-99 -0.23092327008712488 2307 100-101 -0.1781344472591151 2201 1 0.08175868298021527 2201 2 0.06788856054530612 2201 3 0.08494493897871536 2201 4 0.029738083930119785 2201 5 0.05684824039280301 2201 6 0.07942649295335258 2201 7 0.060694793647556367 2201 8 0.07568370083557596 2201 9 0.07104153323111717 2201 10-11 0.09105127112667333 2201 12-13 0.10646912899131422 2201 14-15 0.17428593011016602 2201 16-17 0.15814203123253634 2201 18-19 0.14016805481136174 2201 20-21 0.13216956916787836 2201 22-23 0.08909245833045532 2201 24-25 0.10578765318609129 2201 26-27 0.14183020509979372 2201 28-29 0.12842300282415664 2201 30-31 0.08454534788065615 2201 32-33 0.07934027001639521 2201 34-35 0.1386633203795853 2201 36-37 0.10946426380360208 2201 38-39 0.08064507915665331 2201 40-41 0.07817843823384862 2201 42-43 0.05968695202316354 2201 44-45 0.03695118380597506 2201 46-47 0.035125479996125364 2201 48-49 -0.019171008563127145 2201 50-51 -0.05279008131926588 2201 52-53 -0.030510567852125803 2201 54-55 0.010229503280363872 2201 56-57 -0.011283776945688828 2201 58-59 -0.1103538149054657 2201 60-61 -0.06867387833569438 2201 62-63 -0.13622637502680846 2201 64-65 -0.1344721094365724 2201 66-67 -0.07802098231388044 2201 68-69 -0.20013104430241668 2201 70-71 -0.3900597913851982 2201 72-73 -0.41189757344533007 2201 74-75 -0.33031773972614786 2201 76-77 -0.8534132477990326 2201 78-79 -0.22304008108685736 2201 80-81 0.0016183003974106214 2201 82-83 -0.006116283490371188 2201 84-85 -0.02286708409969407 2201 86-87 -0.04271721458464839 2201 88-89 -0.07015610011733031 2201 90-91 -0.09581021041569215 2201 92-93 -0.018771309251782498 2201 94-95 -0.2098460972854994 2201 96-97 -0.31207737757733156 2201 98-99 -0.1947730491949855 2201 100-101 -0.2893127270688005 1101 1 0.08949098446079518 1101 2 0.07494187064821745 1101 3 0.06249817961636239 1101 4 0.041138523588735154 1101 5 0.040536653121499455 1101 6 0.045612025698744674 1101 7 0.03888902916072823 1101 8 0.06965403617664379 1101 9 0.059595120197968754 1101 10-11 0.049144559319778125 1101 12-13 0.06724521220073143 1101 14-15 0.0897031371545367 1101 16-17 0.0906734983322579 1101 18-19 0.12027183392113017 1101 20-21 0.11929808788261909 1101 22-23 0.085809762626198 1101 24-25 0.013945733525169146 1101 26-27 0.02095669768895192 1101 28-29 0.028961800669605964 1101 30-31 0.03768031855567244 1101 32-33 0.04204070721547026 1101 34-35 0.07265718749419392 1101 36-37 -0.02833427895475893 1101 38-39 -0.09274555027840137 1101 40-41 0.0017766192410491044 1101 42-43 -0.02333121729791543 1101 44-45 -0.052798920724292486 1101 46-47 -0.009352483561535507 1101 48-49 -0.05182199580080038 1101 50-51 -0.14114810196473826 1101 52-53 -0.07969995143266573 1101 54-55 -0.07900572072683332 1101 56-57 -0.05222024846924711 1101 58-59 -0.0891933661000337 1101 60-61 -0.023312037433079524 1101 62-63 -0.17027725018942164 1101 64-65 -0.20027638756618416 1101 66-67 -0.11834792772348379 1101 68-69 -0.2790448601789137 1101 70-71 -0.28905543667762146 1101 72-73 -0.2648707598535367 1101 74-75 -0.2378878580962862 1101 76-77 -0.6823489003636993 1101 78-79 -0.16303049554520044 1101 80-81 0.019165997108750332 1101 82-83 -9.766679969658298E-5 1101 84-85 0.028805233959481313 1101 86-87 -0.12021738243133484 1101 88-89 -0.05790910158174967 1101 90-91 -0.15594043285743453 1101 92-93 -0.10610774514531585 1101 94-95 -0.13961157058989926 1101 96-97 -0.22249187580879237 1101 98-99 -0.20805460819493504 1101 100-101 -0.3270848929691361 2304 1 0.06763748780227274 2304 2 0.04696470075647596 2304 3 0.06056408825735815 2304 4 -0.006601888339403672 2304 5 0.02130934571496823 2304 6 0.06139682529550328 2304 7 0.02945745520865728 2304 8 0.019409637868754714 2304 9 0.04570090299811369 2304 10-11 0.03361659447477905 2304 12-13 0.06178318653628878 2304 14-15 0.07805558076827168 2304 16-17 0.06077857277318088 2304 18-19 0.0743259039202826 2304 20-21 0.06329914843835382 2304 22-23 0.08177412543481921 2304 24-25 0.05994397740112589 2304 26-27 0.05640824719251469 2304 28-29 0.02490199458690512 2304 30-31 0.02394657287874935 2304 32-33 0.046091172951250314 2304 34-35 0.059752687633334745 2304 36-37 0.015819682683485325 2304 38-39 -0.08599430885259096 2304 40-41 -0.06203569640298667 2304 42-43 -0.060915211631836996 2304 44-45 -0.042862073169708026 2304 46-47 -0.050187984534552754 2304 48-49 -0.031072268859695384 2304 50-51 -0.09746340104150875 2304 52-53 -0.07551781109812339 2304 54-55 -0.10007959835946423 2304 56-57 -0.09578133852739512 2304 58-59 -0.15461414253173444 2304 60-61 -0.13977098301401014 2304 62-63 -0.12634548618310504 2304 64-65 -0.11730434569346926 2304 66-67 -0.1390386230771128 2304 68-69 -0.20505416423532097 2304 70-71 -0.2803250382062217 2304 72-73 -0.30451996735931175 2304 74-75 -0.39077822944300067 2304 76-77 -0.746975625842822 2304 78-79 -0.24951204139443206 2304 80-81 -0.04070747341563674 2304 82-83 -0.06017405437788881 2304 84-85 -0.1434798372956223 2304 86-87 -0.22093410532599478 2304 88-89 -0.16305370914017914 2304 90-91 -0.14499941868513844 2304 92-93 -0.11337914325296339 2304 94-95 -0.18743459054914524 2304 96-97 -0.24661659135333736 2304 98-99 -0.25175776919341075 2304 100-101 -0.30902731776186343 2202 1 0.08242680568274352 2202 2 0.07445744914860342 2202 3 0.06926484744222705 2202 4 0.042639358671330285 2202 5 0.017374131853621577 2202 6 0.03872534917068293 2202 7 0.07247781989580204 2202 8 0.035690515262736255 2202 9 0.04017476848252244 2202 10-11 0.0502499308563813 2202 12-13 0.06972626826191686 2202 14-15 0.10701415675761439 2202 16-17 0.08711987712137415 2202 18-19 0.06430231247590967 2202 20-21 0.0530513674991866 2202 22-23 0.04149523381475717 2202 24-25 0.025411634291003793 2202 26-27 0.071452836270673 2202 28-29 0.011246190606208017 2202 30-31 0.04886968223490129 2202 32-33 0.07533408173944878 2202 34-35 0.041696029380879907 2202 36-37 -0.06527271699171422 2202 38-39 -0.046610893491987326 2202 40-41 0.031215528294126216 2202 42-43 0.008945185686791035 2202 44-45 -0.01060042190569277 2202 46-47 0.01849931882247091 2202 48-49 0.0010637878048029847 2202 50-51 -0.029755387267371702 2202 52-53 -0.017256419856018113 2202 54-55 0.010634625458116886 2202 56-57 0.011376071308781377 2202 58-59 -0.025335978092563494 2202 60-61 -0.08638530926327803 2202 62-63 -0.14922631450393453 2202 64-65 -0.05970089461300887 2202 66-67 -0.039850040814584986 2202 68-69 -0.1417210499088739 2202 70-71 -0.1938902914759879 2202 72-73 -0.21782533802679893 2202 74-75 -0.15277218630176748 2202 76-77 -0.21138665808359036 2202 78-79 -0.02949728880060576 2202 80-81 0.04929906391213024 2202 82-83 -0.05441121966862994 2202 84-85 -0.06359611323561154 2202 86-87 -0.03435774807260117 2202 88-89 -0.04197803534988509 2202 90-91 -0.01276478662980196 2202 92-93 0.03975781557502245 2202 94-95 -0.04776229228471607 2202 96-97 -0.201288281358174 2202 98-99 -0.0918137920472546 2202 100-101 -0.04463605744075139 1102 1 0.08816200328778478 1102 2 0.07044626234785056 1102 3 0.06516487733800602 1102 4 -0.019442640626238017 1102 5 0.03927099993506289 1102 6 0.0432021126884905 1102 7 0.03408889144025551 1102 8 0.023155269101231113 1102 9 0.018832965880470454 1102 10-11 0.019734471927435493 1102 12-13 0.0735036346439557 1102 14-15 0.08186221006480565 1102 16-17 0.06612580041521454 1102 18-19 0.05320257057456246 1102 20-21 0.047234049257660615 1102 22-23 0.038203706495806955 1102 24-25 0.02901118584910023 1102 26-27 -0.010094575711711684 1102 28-29 0.006536743242300247 1102 30-31 -0.005268954853072216 1102 32-33 0.019971303703094634 1102 34-35 0.035555401958312416 1102 36-37 -0.0677120761403529 1102 38-39 -0.07910827375746265 1102 40-41 -0.02403577417888414 1102 42-43 -0.04863799797169577 1102 44-45 -0.06646429781892493 1102 46-47 -0.06082630274818257 1102 48-49 -0.09762630051394439 1102 50-51 -0.1239449093639351 1102 52-53 -0.03478657600124535 1102 54-55 0.006371785806457808 1102 56-57 -0.009198962836734381 1102 58-59 -0.06666954407054959 1102 60-61 -0.16528011756162897 1102 62-63 -0.19750453503989007 1102 64-65 -0.15698116259957828 1102 66-67 -0.16333135119761977 1102 68-69 -0.1796395026275306 1102 70-71 -0.25614661749538215 1102 72-73 -0.27098319134371707 1102 74-75 -0.31086645661196144 1102 76-77 -0.5184998363110793 1102 78-79 -0.15325016165115812 1102 80-81 -0.08135954568280113 1102 82-83 -0.11243984833865284 1102 84-85 -0.1586308272982393 1102 86-87 -0.21489683203479615 1102 88-89 -0.19575155306193892 1102 90-91 -0.33187582120298487 1102 92-93 -0.22319725699030357 1102 94-95 -0.3719920971271051 1102 96-97 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-1.2300172087923045 1213 96-97 -0.48877362545447056 1213 98-99 -0.21059706006543877 1213 100-101 -0.0013345899177288345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 31.0 8 434.0 9 1207.0 10 779.0 11 274.0 12 204.0 13 360.0 14 779.0 15 1846.0 16 3424.0 17 5526.0 18 7993.0 19 11506.0 20 15965.0 21 21064.0 22 27961.0 23 36809.0 24 47966.0 25 61775.0 26 80042.0 27 103930.0 28 135314.0 29 175804.0 30 232662.0 31 313261.0 32 433881.0 33 632765.0 34 1037220.0 35 2047268.0 36 4449964.0 37 5948661.0 38 934274.0 39 44.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.422810003271394 20.919767751425756 31.80579560830764 27.851626636995213 2 28.427636114895254 21.37112659432233 31.130021667763703 19.071215623018713 3 20.723574156026622 19.504547046308577 40.026839191492314 19.74503960617249 4 20.67582890403262 19.824808707567314 38.84208945613754 20.657272932262526 5 29.97295867383436 18.9813521521708 32.140426714046875 18.90526245994797 6 30.560610081797762 19.716466468868845 31.435441792756862 18.287481656576528 7 29.784693181925913 27.118876713173346 24.173524090428256 18.922906014472485 8 27.02830964670735 21.14514718474996 31.75096268874176 20.075580479800934 9 22.87124015346255 20.387529733622873 35.71199136223088 21.029238750683696 10-11 28.55346127581641 21.5387443076785 28.0828140449429 21.82498037156219 12-13 27.14621992785984 26.107263488685774 23.99134444456171 22.75517213889268 14-15 26.44827053054602 23.769530920418187 25.81832662633024 23.96387192270556 16-17 25.950878173790144 23.297449161788055 25.24418494619014 25.50748771823166 18-19 26.106682864471324 24.08394384662999 25.722090344420188 24.087282944478495 20-21 24.936558260830264 24.54881816413062 26.055499469646797 24.45912410539232 22-23 25.092172516636907 24.462517046541716 25.74608712886896 24.699223307952415 24-25 25.676091290219656 24.45218373123554 25.19390469153384 24.677820287010963 26-27 25.382116519369717 24.63595634773532 25.493368104472264 24.4885590284227 28-29 25.080436563635516 25.327819244909954 25.086190550582018 24.505553640872506 30-31 24.984113189518506 25.205152648286216 25.0301897845908 24.78054437760448 32-33 24.883881381649523 25.457562242721764 25.098504877199606 24.560051498429104 34-35 24.571186793486397 25.586016144418842 25.143913626601925 24.69888343549283 36-37 24.771958048097975 25.43603798788644 25.058201354759568 24.733802609256017 38-39 24.75847934388132 24.952080471237593 25.261712285338533 25.02772789954255 40-41 24.91431841054346 25.16712963366 24.770476257145297 25.14807569865124 42-43 24.859172664353167 25.163045774216958 24.860141599949987 25.11763996147989 44-45 25.028691013039168 25.15051489985857 24.665795238201145 25.154998848901112 46-47 24.687669492865865 25.082992711465753 25.145951517128022 25.08338627854036 48-49 24.835405179564397 24.848240333228617 24.9630381861176 25.35331630108939 50-51 24.80940463944013 25.250202763559308 25.36621843271096 24.5741741642896 52-53 25.158938099111065 24.782856151330776 25.474447070721045 24.583758678837114 54-55 24.540804439815197 25.12276749975417 24.98951233912435 25.346915721306278 56-57 24.686466725957494 25.326248887477004 25.28912329177459 24.698161094790912 58-59 24.820419820917657 25.481332371600445 25.086852513227925 24.611395294253974 60-61 25.153106597399194 25.463690901396063 24.866605137192302 24.516597364012437 62-63 24.812067804550278 25.626541031480038 25.19863011738137 24.362761046588314 64-65 25.035264503379572 25.454329855468682 24.872076819474394 24.638328821677344 66-67 25.597856401504547 25.261800131894947 25.07547131270061 24.0648721538999 68-69 25.011551834159317 24.850747559324663 25.7460206704477 24.39167993606832 70-71 25.0871173954182 25.12456768545072 25.31865918062403 24.469655738507047 72-73 24.631662847642634 24.836376848737874 25.791092601679 24.740867701940495 74-75 24.662046751650582 24.87870516420904 25.76560133802434 24.693646746116034 76-77 24.29788642622128 24.889012346853562 25.388850089565846 25.424251137359317 78-79 25.237297028136723 25.113308149570635 24.86142778971548 24.787967032577164 80-81 24.766493006849778 24.959496833535162 25.48923251694556 24.7847776426695 82-83 24.65134135439076 25.686153524348583 25.389053325091215 24.273451796169443 84-85 24.829514750780078 25.390664925567783 25.13389199883646 24.64592832481568 86-87 24.61341843508494 25.504028840369358 25.371813995719567 24.51073872882613 88-89 24.729965927722304 26.464731626244554 24.64313326595055 24.16216918008259 90-91 24.70408548214541 25.325142391766587 24.870700615233105 25.1000715108549 92-93 24.515332532847676 25.78969830660485 24.943272094570148 24.751697065977325 94-95 24.398442263409102 26.01925594324344 24.504478336131903 25.077823457215555 96-97 24.379926382664554 25.067412774634683 25.104910622623823 25.447750220076937 98-99 24.743491468303997 25.18397953567904 24.816672051831176 25.25585694418579 100-101 24.71949985143818 26.375766791911538 24.036130426274344 24.868602930375943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2630.0 1 1940.0 2 1581.0 3 1843.0 4 1869.0 5 1745.5 6 1480.0 7 1420.5 8 1430.5 9 1457.0 10 1480.5 11 1464.0 12 1459.5 13 1482.5 14 1544.0 15 1695.0 16 1802.5 17 1958.0 18 2173.5 19 2330.5 20 2534.5 21 2806.0 22 3100.0 23 3591.0 24 4343.0 25 5040.0 26 5880.0 27 6978.0 28 8093.5 29 9335.5 30 11291.0 31 14180.0 32 18901.5 33 26052.0 34 35902.5 35 50724.5 36 69551.0 37 94630.5 38 126958.0 39 159879.0 40 203051.0 41 275988.5 42 367639.5 43 457127.0 44 579961.5 45 748455.0 46 907482.0 47 1084493.0 48 1235896.0 49 1247824.5 50 1178163.5 51 1137033.5 52 1081561.0 53 1037249.5 54 1001853.5 55 890431.0 56 744921.0 57 562884.5 58 386638.5 59 277675.5 60 208256.0 61 154439.0 62 115920.5 63 78456.5 64 43917.5 65 24067.5 66 13387.5 67 8025.5 68 6023.5 69 4600.5 70 3318.0 71 2427.5 72 1943.5 73 1591.0 74 1260.0 75 981.5 76 713.5 77 497.0 78 371.0 79 340.0 80 264.5 81 152.0 82 102.0 83 77.5 84 64.0 85 52.5 86 37.5 87 27.0 88 26.0 89 24.0 90 19.0 91 14.0 92 10.0 93 6.5 94 4.5 95 2.5 96 1.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15404570163598502 2 0.0 3 0.0 4 0.09709023262004042 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.951523991090095E-4 12-13 2.027309408021479E-4 14-15 0.0012998630910255366 16-17 0.0 18-19 0.0 20-21 5.84342123488544E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.0136547040107394E-4 30-31 5.6645409930011916E-5 32-33 0.0 34-35 0.0 36-37 7.423530038196298E-4 38-39 0.002584819495227386 40-41 0.0010911694754939137 42-43 9.838413303633649E-5 44-45 3.9353653214534596E-4 46-47 0.007760421160411632 48-49 0.003866794562064497 50-51 0.005059329507959485 52-53 0.0018901678892435554 54-55 0.00769185040102267 56-57 0.013371298080847548 58-59 0.019960053656917356 60-61 0.010720889163595938 62-63 0.00825830450032279 64-65 0.00987120801464576 66-67 0.0230427564920559 68-69 0.01846640363718388 70-71 0.012530560943991582 72-73 0.01770616260917583 74-75 0.021400039604085555 76-77 0.03551667202611747 78-79 8.228491126675415E-4 80-81 5.127900267348448E-4 82-83 0.0013356391394023861 84-85 0.009009601516236632 86-87 0.016901201520696713 88-89 0.01856180643285548 90-91 0.021540162460228215 92-93 0.021918792305549873 94-95 0.029840205683657327 96-97 0.02803351524062642 98-99 0.025368199636551125 100-101 0.3022181686634372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6770997E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.37714387747152 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17607108314692 13.447671866830744 2 10.137118167486108 3.725825581336453 3 4.0028253881088895 2.206814942210185 4 2.344113178008515 1.72312420549358 5 1.507986805713322 1.3856245246961207 6 1.1036979276438583 1.2169689368147 7 0.7973267808470266 1.0256812278290908 8 0.6518342930635799 0.9583082070319475 9 0.4772863935346358 0.7894044652270946 >10 4.363942405331682 16.79930517871983 >50 0.735171723386897 9.41510457130333 >100 0.5848921519330879 21.46517122458287 >500 0.06623897699469472 8.510740047425717 >1k 0.05083288323911516 16.15841525915382 >5k 3.685863065143716E-4 0.48269558685548164 >10k+ 2.932552551913536E-4 0.6891441744890516 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.75147714831742E-5 0.0 0.0 2 7.75147714831742E-5 0.0 0.0 3 7.75147714831742E-5 0.0 0.0 4 7.75147714831742E-5 0.0 0.0 5 7.75147714831742E-5 0.0 0.0 6 8.347744621264913E-5 0.0 0.0 7 8.944012094212407E-5 0.0 0.0 8 9.540279567159901E-5 0.0 0.0 9 1.1925349458949877E-4 0.0 0.0 10-11 1.341601814131861E-4 0.0 0.0 12-13 1.639735550605608E-4 0.0 0.0 14-15 2.2956297708478512E-4 0.0 0.0 16-17 3.488164716742839E-4 0.0 0.0 18-19 4.8297665308747E-4 0.0 0.0 20-21 6.320435213243434E-4 0.0 0.0 22-23 7.960170763849043E-4 0.0 0.0 24-25 9.361399325275653E-4 0.0 0.0 26-27 0.0011776282590713004 0.0 0.0 28-29 0.001493650019733472 0.0 0.0 30-31 0.0018037091056661687 0.0 0.0 32-33 0.002161469589434665 0.0 0.0 34-35 0.002590782169956861 0.0 0.0 36-37 0.00313338557033908 0.0 0.0 38-39 0.0038041864774050107 0.0 0.0 40-41 0.004579334192236753 0.0 0.0 42-43 0.005402183304904294 0.0 0.0 44-45 0.006600680925528757 0.0 0.0 46-47 0.00801085349904958 0.0 0.0 48-49 0.009820525279445224 0.0 0.0 50-51 0.012023733591986212 0.0 0.0 52-53 0.014808302690651007 0.0 0.0 54-55 0.018454478287724934 0.0 0.0 56-57 0.022365992910260493 0.0 0.0 58-59 0.02730606892363048 0.0 0.0 60-61 0.033724888269910255 0.0 0.0 62-63 0.04212033428901096 0.0 0.0 64-65 0.05271898862065266 0.0 0.0 66-67 0.06561625406050696 0.0 0.0 68-69 0.0815246702387461 0.0 0.0 70-71 0.10181863368051405 0.0 0.0 72-73 0.1266650992782361 0.0 0.0 74-75 0.15797808562007376 0.0 0.0 76-77 0.20035779625981687 0.0 0.0 78-79 0.25490434468505363 0.0 0.0 80-81 0.31795068593715686 0.0 0.0 82-83 0.39968703112879933 0.0 0.0 84-85 0.49271370092070255 0.0 0.0 86-87 0.603100698187472 0.0 0.0 88-89 0.7338502296553986 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 179680 0.0 73.96975 1 TTTGTGT 191060 0.0 69.62971 2 TTGTGTT 218390 0.0 62.4873 3 TAACTTT 17305 0.0 61.252647 1 TGTGTTT 232505 0.0 59.012688 4 AACTTTG 18290 0.0 58.571472 2 GTGTTTG 252290 0.0 54.391655 5 ACTTTGT 38985 0.0 50.862534 1 TTTGAGG 86545 0.0 38.38463 8 TTGAGGG 42750 0.0 36.97728 9 TTGAGTC 24325 0.0 36.51761 4 GTTTGAA 22400 0.0 36.282234 2 GTTTGAG 220820 0.0 36.186253 7 TGTTTGA 386030 0.0 35.536613 6 TTGAGGT 22690 0.0 33.275143 9 TTTGAGC 53010 0.0 33.232704 8 TTTGAGT 84550 0.0 33.204678 3 TTTGAGA 25485 0.0 32.81136 8 TGAGTCT 5570 0.0 31.627747 5 GTTTGAC 70930 0.0 31.310278 7 >>END_MODULE