FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30348.34100000035cf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30348.34100000035cf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16770997
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1796800.073.969751
TTTGTGT1910600.069.629712
TTGTGTT2183900.062.48733
TAACTTT173050.061.2526471
TGTGTTT2325050.059.0126884
AACTTTG182900.058.5714722
GTGTTTG2522900.054.3916555
ACTTTGT389850.050.8625341
TTTGAGG865450.038.384638
TTGAGGG427500.036.977289
TTGAGTC243250.036.517614
GTTTGAA224000.036.2822342
GTTTGAG2208200.036.1862537
TGTTTGA3860300.035.5366136
TTGAGGT226900.033.2751439
TTTGAGC530100.033.2327048
TTTGAGT845500.033.2046783
TTTGAGA254850.032.811368
TGAGTCT55700.031.6277475
GTTTGAC709300.031.3102787
Produced by FastQC (version 0.11.2)