##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l06n01_30304.34100000035cbf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19504740 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7328268410653 33.0 33.0 33.0 33.0 33.0 2 32.76373640458678 33.0 33.0 33.0 33.0 33.0 3 32.78289256867818 33.0 33.0 33.0 33.0 33.0 4 36.699116727523666 37.0 37.0 37.0 37.0 37.0 5 36.723636408380735 37.0 37.0 37.0 37.0 37.0 6 36.67198393826321 37.0 37.0 37.0 37.0 37.0 7 36.63564989843494 37.0 37.0 37.0 37.0 37.0 8 36.625752919546734 37.0 37.0 37.0 37.0 37.0 9 36.618316060608855 37.0 37.0 37.0 37.0 37.0 10-11 36.606436768703404 37.0 37.0 37.0 37.0 37.0 12-13 36.5642760170092 37.0 37.0 37.0 37.0 37.0 14-15 38.658413518970264 40.0 37.0 40.0 37.0 40.0 16-17 38.61642136731892 40.0 37.0 40.0 37.0 40.0 18-19 38.58249558825188 40.0 37.0 40.0 37.0 40.0 20-21 38.52751326087915 40.0 37.0 40.0 37.0 40.0 22-23 38.46252716006468 40.0 37.0 40.0 37.0 40.0 24-25 38.42108897632063 40.0 37.0 40.0 37.0 40.0 26-27 38.32641768103548 40.0 37.0 40.0 37.0 40.0 28-29 38.26440283233717 40.0 37.0 40.0 37.0 40.0 30-31 38.1907335345152 40.0 37.0 40.0 37.0 40.0 32-33 38.07813523789602 40.0 37.0 40.0 37.0 40.0 34-35 37.973711518328365 40.0 37.0 40.0 35.0 40.0 36-37 37.87489482043851 40.0 37.0 40.0 33.0 40.0 38-39 37.76398044782961 40.0 37.0 40.0 33.0 40.0 40-41 37.5970028054719 40.0 37.0 40.0 33.0 40.0 42-43 37.43136194073851 40.0 37.0 40.0 33.0 40.0 44-45 37.269712182782236 38.5 37.0 40.0 33.0 40.0 46-47 37.04249097911584 37.0 37.0 40.0 33.0 40.0 48-49 36.796788472955804 37.0 37.0 40.0 33.0 40.0 50-51 36.55379497496506 37.0 37.0 40.0 33.0 40.0 52-53 36.26974166279581 37.0 37.0 40.0 33.0 40.0 54-55 36.00558623185954 37.0 37.0 40.0 33.0 40.0 56-57 35.7136991315957 37.0 33.0 38.5 33.0 40.0 58-59 35.433552587729956 37.0 33.0 37.0 33.0 40.0 60-61 35.145653338624356 37.0 33.0 37.0 33.0 40.0 62-63 34.880248288364776 37.0 33.0 37.0 27.0 40.0 64-65 34.62458538283515 37.0 33.0 37.0 27.0 38.5 66-67 34.356121435097315 37.0 33.0 37.0 27.0 37.0 68-69 34.1379763585672 37.0 33.0 37.0 27.0 37.0 70-71 33.92770393248 37.0 33.0 37.0 27.0 37.0 72-73 33.71743422368101 37.0 33.0 37.0 27.0 37.0 74-75 33.45620331263068 37.0 33.0 37.0 27.0 37.0 76-77 30.491925988246958 33.0 30.0 33.0 24.5 35.0 78-79 33.271906034122985 35.0 33.0 37.0 27.0 37.0 80-81 33.79437713601924 37.0 33.0 37.0 27.0 37.0 82-83 33.796971659196686 37.0 33.0 37.0 27.0 37.0 84-85 33.688070130645166 37.0 33.0 37.0 27.0 37.0 86-87 33.60049769953355 37.0 33.0 37.0 27.0 37.0 88-89 33.44095263510306 37.0 33.0 37.0 27.0 37.0 90-91 33.30595196347144 37.0 33.0 37.0 27.0 37.0 92-93 33.16509999620605 37.0 33.0 37.0 27.0 37.0 94-95 33.033885481170216 33.0 33.0 37.0 27.0 37.0 96-97 32.95687494424432 33.0 33.0 37.0 27.0 37.0 98-99 32.79103074432164 33.0 33.0 37.0 27.0 37.0 100-101 30.721725231917986 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1150802877604562 2306 2 0.0774581430805199 2306 3 0.08855929010879748 2306 4 0.08953194906442263 2306 5 0.0920474252799437 2306 6 0.10260764402844558 2306 7 0.1475069852916704 2306 8 0.13133757892705233 2306 9 0.09709962153741714 2306 10-11 0.11563894368849503 2306 12-13 0.1549491117366486 2306 14-15 0.27200584521536086 2306 16-17 0.26697414595216173 2306 18-19 0.2443316821288093 2306 20-21 0.23620959565674582 2306 22-23 0.22493893965854284 2306 24-25 0.22590095083109674 2306 26-27 0.24839937788057398 2306 28-29 0.18798437688867864 2306 30-31 0.22037295383344002 2306 32-33 0.2456721020685535 2306 34-35 0.21509284496665515 2306 36-37 0.2724227977910232 2306 38-39 0.2556353024948095 2306 40-41 0.22374624868432136 2306 42-43 0.2344523342997462 2306 44-45 0.21395767861803705 2306 46-47 0.280821081112137 2306 48-49 0.22601968323561294 2306 50-51 0.24179480803435638 2306 52-53 0.2518175180597453 2306 54-55 0.25632581852852354 2306 56-57 0.23391448412297677 2306 58-59 0.20180857268117336 2306 60-61 0.12381197615376749 2306 62-63 0.14500279298686536 2306 64-65 0.20319669368798543 2306 66-67 0.23611352106807004 2306 68-69 0.1701397235631319 2306 70-71 0.1157247579277012 2306 72-73 0.056699914446745936 2306 74-75 0.045480070131233674 2306 76-77 -0.242069019787035 2306 78-79 0.13219766480860073 2306 80-81 0.26792213196136316 2306 82-83 0.24659254235579908 2306 84-85 0.27914324326292217 2306 86-87 0.2528662918647555 2306 88-89 0.28471108319721594 2306 90-91 0.11794490781355194 2306 92-93 0.25703170167049194 2306 94-95 0.21370051174746152 2306 96-97 0.13191174101714154 2306 98-99 0.3026544167025449 2306 100-101 0.2575598046691461 2307 1 0.08241956344676282 2307 2 0.07190070122823755 2307 3 0.05686861515799535 2307 4 0.034478907260840685 2307 5 0.04786529049114563 2307 6 0.08358499176576828 2307 7 0.06155398280989033 2307 8 0.09662789534653626 2307 9 0.07327441770296161 2307 10-11 0.08468471897640484 2307 12-13 0.09706620679899913 2307 14-15 0.13594738417612007 2307 16-17 0.13118121369186753 2307 18-19 0.1287266279566026 2307 20-21 0.12818376780521135 2307 22-23 0.11633164054875778 2307 24-25 0.10465979065906339 2307 26-27 0.08883510978541409 2307 28-29 0.1186652406322608 2307 30-31 0.06782270998778017 2307 32-33 0.07287927361814184 2307 34-35 0.010960550655234158 2307 36-37 0.024462474698211167 2307 38-39 0.00907056762677172 2307 40-41 0.05397171502400511 2307 42-43 0.06224119468199518 2307 44-45 -0.0035852589324960604 2307 46-47 0.04760746454915221 2307 48-49 -0.09439628225476326 2307 50-51 -0.10384585118655565 2307 52-53 -0.04352542017138461 2307 54-55 -0.05151345174042632 2307 56-57 -0.04335751384213182 2307 58-59 -0.169127817090434 2307 60-61 -0.1775532235275037 2307 62-63 -0.20676585417597693 2307 64-65 -0.23775902343783173 2307 66-67 -0.22245135298891938 2307 68-69 -0.3769558009284637 2307 70-71 -0.3715252207255588 2307 72-73 -0.4884735479925908 2307 74-75 -0.46229071700543045 2307 76-77 -0.5392023948907436 2307 78-79 -0.24196036625667716 2307 80-81 -0.14244811972909588 2307 82-83 -0.21176583403575222 2307 84-85 -0.289732603482868 2307 86-87 -0.20966293693383165 2307 88-89 -0.20361023670435685 2307 90-91 -0.33585199433374413 2307 92-93 -0.23543394367357706 2307 94-95 -0.3808347863893147 2307 96-97 -0.5064702630744691 2307 98-99 -0.4080873259234963 2307 100-101 -0.3405253127449548 2201 1 0.07507318224079285 2201 2 0.08648671851721446 2201 3 0.09181508736408261 2201 4 0.04984816129118741 2201 5 0.0706838835270247 2201 6 0.08662624289218002 2201 7 0.07484715312222079 2201 8 0.08625098144374022 2201 9 0.036037641027377276 2201 10-11 0.07588559486994484 2201 12-13 0.08869916802672151 2201 14-15 0.1280574590129362 2201 16-17 0.1208736630300038 2201 18-19 0.1633799326332408 2201 20-21 0.12689615611829907 2201 22-23 0.09502970312629344 2201 24-25 0.13407945792074116 2201 26-27 0.16405986851421517 2201 28-29 0.11156513545685698 2201 30-31 0.09080811918307319 2201 32-33 0.04113812060558075 2201 34-35 0.11567255493112327 2201 36-37 0.12027338846847613 2201 38-39 0.09724484381317922 2201 40-41 0.06471251635032615 2201 42-43 0.0658985337494471 2201 44-45 0.06577671447953293 2201 46-47 0.05884506182596283 2201 48-49 0.03350008961987072 2201 50-51 0.030351746302464733 2201 52-53 -0.007890844352466786 2201 54-55 -0.0053041885126674515 2201 56-57 0.011431802270749358 2201 58-59 -0.00731697732734915 2201 60-61 -0.016807295617617513 2201 62-63 -0.05604599748725292 2201 64-65 -0.08291189085472439 2201 66-67 -0.06679316357821818 2201 68-69 -0.2678979452370527 2201 70-71 -0.40264340469573057 2201 72-73 -0.33859435969556984 2201 74-75 -0.3588336277708706 2201 76-77 -0.8727394849184229 2201 78-79 -0.19625533448229504 2201 80-81 0.05914152338937839 2201 82-83 -0.037641484076978315 2201 84-85 -0.033128159365411136 2201 86-87 -0.06657749370045707 2201 88-89 -0.0666629445927569 2201 90-91 -0.1093970446977508 2201 92-93 0.00915411250856124 2201 94-95 -0.2221526040457391 2201 96-97 -0.31574216123921417 2201 98-99 -0.2810402210996088 2201 100-101 -0.30379276752275075 1101 1 0.08774179882450284 1101 2 0.07165605184272295 1101 3 0.06346536335931319 1101 4 0.019247938165229073 1101 5 0.03585382972086393 1101 6 0.050120213518482615 1101 7 0.061213353880532395 1101 8 0.046224637984309425 1101 9 0.04249186761688861 1101 10-11 0.05771270550538077 1101 12-13 0.06878380233705883 1101 14-15 0.09296864516584691 1101 16-17 0.10278122695723368 1101 18-19 0.11066977140086465 1101 20-21 0.09972035748636188 1101 22-23 0.08383778902095429 1101 24-25 0.015692325437008492 1101 26-27 0.047243208153055605 1101 28-29 0.03663942993566138 1101 30-31 0.0702392327535648 1101 32-33 0.0411118711904237 1101 34-35 0.05649314571050468 1101 36-37 -0.026758848030105753 1101 38-39 -0.09948143763433137 1101 40-41 -0.04177470871367461 1101 42-43 -0.043011965169029054 1101 44-45 -0.12533651894993625 1101 46-47 -0.05764295332844682 1101 48-49 -0.03291995582919327 1101 50-51 -0.1240425383665027 1101 52-53 -0.11193944421803081 1101 54-55 -0.062356278823379796 1101 56-57 -0.03447733227481109 1101 58-59 -0.09580993589111131 1101 60-61 -0.08093359541167189 1101 62-63 -0.18978632715312216 1101 64-65 -0.21534353721760624 1101 66-67 -0.2080708292635265 1101 68-69 -0.3307077833187009 1101 70-71 -0.3883739709068408 1101 72-73 -0.35240424102573087 1101 74-75 -0.35189793008557757 1101 76-77 -0.7293423518342621 1101 78-79 -0.18661723322898638 1101 80-81 -0.0718249448695687 1101 82-83 -0.06825688073210046 1101 84-85 -0.03533512744910183 1101 86-87 -0.15942562656904613 1101 88-89 -0.15833866746571346 1101 90-91 -0.24942862381503517 1101 92-93 -0.18002981063941093 1101 94-95 -0.1683901763474509 1101 96-97 -0.2702106124591168 1101 98-99 -0.2916265790181498 1101 100-101 -0.36936663780460677 2304 1 0.07755718282938773 2304 2 0.06290224206602346 2304 3 0.07863311085976221 2304 4 0.02764001044420894 2304 5 0.041267030147338346 2304 6 0.03930002661987686 2304 7 0.04792032219238962 2304 8 0.03789397679238249 2304 9 0.06835551557077224 2304 10-11 0.04097430286242343 2304 12-13 0.029030713684427667 2304 14-15 0.05779911677330318 2304 16-17 0.04564234021743374 2304 18-19 0.04438951581257555 2304 20-21 0.08128386580875713 2304 22-23 0.04532580438353051 2304 24-25 -0.027138697788593902 2304 26-27 0.024556647629886186 2304 28-29 0.019940700576746906 2304 30-31 0.03512942859308055 2304 32-33 0.03338505441568884 2304 34-35 0.01716218469988462 2304 36-37 -0.04704244591644624 2304 38-39 -0.12931442828470097 2304 40-41 -0.09130841987038707 2304 42-43 -0.03417144699911745 2304 44-45 -0.05212591439130421 2304 46-47 -0.06809693449513077 2304 48-49 -0.07169909451530287 2304 50-51 -0.14625257615289655 2304 52-53 -0.14075380807850735 2304 54-55 -0.09860870741168526 2304 56-57 -0.06621943623078863 2304 58-59 -0.16774768971334453 2304 60-61 -0.22691126284234997 2304 62-63 -0.15820816453097564 2304 64-65 -0.175436628361048 2304 66-67 -0.16652294162299341 2304 68-69 -0.28035536620064505 2304 70-71 -0.33596459693337977 2304 72-73 -0.3696629271396503 2304 74-75 -0.5077648667260561 2304 76-77 -0.8820921037111411 2304 78-79 -0.32556571891093 2304 80-81 -0.1215999094008211 2304 82-83 -0.10415837174156906 2304 84-85 -0.13068940483524472 2304 86-87 -0.18823183760368778 2304 88-89 -0.17820013771004284 2304 90-91 -0.24659398818121048 2304 92-93 -0.18774203805003964 2304 94-95 -0.19908382726848117 2304 96-97 -0.3470447010957187 2304 98-99 -0.25828562166440605 2304 100-101 -0.3567626229138803 2202 1 0.08352273355284723 2202 2 0.06698216865390094 2202 3 0.07013106298799698 2202 4 0.0028042907761047786 2202 5 0.01770367676031981 2202 6 0.06054865054348113 2202 7 0.046699849687222184 2202 8 0.027569197467229856 2202 9 0.025385672665230175 2202 10-11 0.03755131141912926 2202 12-13 0.06849391529824089 2202 14-15 0.07645831389320534 2202 16-17 0.0641268161319104 2202 18-19 0.07418769180158336 2202 20-21 0.02114471298760634 2202 22-23 -0.01540640320096287 2202 24-25 0.007937247972982675 2202 26-27 0.03981397182600688 2202 28-29 -0.02200561081578911 2202 30-31 -0.0077630175969147786 2202 32-33 0.030764231172057066 2202 34-35 0.00687945141275037 2202 36-37 -0.1088552822774318 2202 38-39 -0.05676376856472132 2202 40-41 0.006584158712577448 2202 42-43 -0.030438132221220826 2202 44-45 -0.005963742180824738 2202 46-47 -0.032330867589827506 2202 48-49 -0.08688896953614034 2202 50-51 -0.13999130765123624 2202 52-53 -0.08866414425113334 2202 54-55 -0.07278445587917304 2202 56-57 0.01211407570284706 2202 58-59 -0.0350116858955829 2202 60-61 -0.0635339717858372 2202 62-63 -0.11408460312063795 2202 64-65 -0.08963774128379498 2202 66-67 -0.06270211774337753 2202 68-69 -0.17998693071598382 2202 70-71 -0.22287457727968274 2202 72-73 -0.21036065859043873 2202 74-75 -0.19583621835185028 2202 76-77 -0.21911229268542698 2202 78-79 -0.03803957749444464 2202 80-81 0.041364640470355596 2202 82-83 -0.04744839122594158 2202 84-85 -0.08392327703675306 2202 86-87 -0.04829963588068864 2202 88-89 -0.1253816724488317 2202 90-91 -0.03756409157774243 2202 92-93 -0.022237953942074284 2202 94-95 -0.10761881713988686 2202 96-97 -0.2513037852751552 2202 98-99 -0.21080516377153913 2202 100-101 -0.07116750365307567 1102 1 0.086629444855852 1102 2 0.06899777805727325 1102 3 0.05559466367877519 1102 4 -0.022937504793837604 1102 5 0.027530026920928208 1102 6 0.0343103076945539 1102 7 0.008081604908156237 1102 8 0.052069986538072044 1102 9 0.02422734653917047 1102 10-11 0.008653300310648149 1102 12-13 0.06341391653111828 1102 14-15 0.03700459431113501 1102 16-17 0.04065330857432059 1102 18-19 0.06873226949857525 1102 20-21 0.05713364956442035 1102 22-23 0.09601543336697205 1102 24-25 0.019181798082726687 1102 26-27 0.012119667071772255 1102 28-29 0.03378083783942287 1102 30-31 -0.002439896162684363 1102 32-33 -0.007097075792984242 1102 34-35 0.022559508687166385 1102 36-37 -0.01997056461763691 1102 38-39 -0.09406143643178666 1102 40-41 -0.07530275143358978 1102 42-43 -0.03871643594575147 1102 44-45 -0.03187674703037402 1102 46-47 -0.010382280378507858 1102 48-49 -0.047557746213328755 1102 50-51 -0.11044761327334385 1102 52-53 -0.013459104547500544 1102 54-55 0.0322572312794307 1102 56-57 0.0012360003236651096 1102 58-59 -0.077592856307092 1102 60-61 -0.1754125558937787 1102 62-63 -0.16564061790108298 1102 64-65 -0.1609791932299487 1102 66-67 -0.12061133863370799 1102 68-69 -0.17284703387132794 1102 70-71 -0.23668671434765542 1102 72-73 -0.2203833366909791 1102 74-75 -0.2780444813563818 1102 76-77 -0.47992783299924113 1102 78-79 -0.14643233227296548 1102 80-81 -0.08073854954395898 1102 82-83 -0.16481040665475888 1102 84-85 -0.16444519241876066 1102 86-87 -0.18589531995616682 1102 88-89 -0.19524954261172667 1102 90-91 -0.30086799075856163 1102 92-93 -0.1925784663508594 1102 94-95 -0.3202722618621934 1102 96-97 -0.34437732211997485 1102 98-99 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40-41 -0.3147599167475619 1213 42-43 -0.22972872855284976 1213 44-45 -0.2518264376150512 1213 46-47 -0.326563834884837 1213 48-49 -0.24738442065275734 1213 50-51 -0.16749753680229418 1213 52-53 -0.15394265456664158 1213 54-55 -0.6804948278868892 1213 56-57 -1.6321740503576265 1213 58-59 -0.5435170190350149 1213 60-61 -0.2988659092270396 1213 62-63 -0.17134116453164694 1213 64-65 -1.5665336005325514 1213 66-67 -0.6678469563336265 1213 68-69 -0.25787427119825423 1213 70-71 -0.10718925185165773 1213 72-73 -0.9242197071979703 1213 74-75 -0.3642169710718619 1213 76-77 -0.3530983093001723 1213 78-79 -0.3926593028714649 1213 80-81 -0.3285259416409261 1213 82-83 -1.292302388170242 1213 84-85 -0.6067256274862274 1213 86-87 -0.4013145029706564 1213 88-89 -1.149220669150445 1213 90-91 -1.4188104923141651 1213 92-93 -2.5286910004854626 1213 94-95 -1.0462350820913926 1213 96-97 -0.35647740831614527 1213 98-99 -0.04748050704611728 1213 100-101 0.2074025527942105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 47.0 8 495.0 9 1447.0 10 943.0 11 328.0 12 269.0 13 575.0 14 1391.0 15 3246.0 16 6099.0 17 9513.0 18 13351.0 19 18584.0 20 24283.0 21 32160.0 22 41673.0 23 54621.0 24 69469.0 25 88506.0 26 113361.0 27 144922.0 28 185620.0 29 239351.0 30 315880.0 31 422311.0 32 582179.0 33 852414.0 34 1384814.0 35 2625387.0 36 5340220.0 37 6153118.0 38 778115.0 39 45.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 22.21335824922201 22.44989213635739 28.511170557241055 26.82557905717954 2 26.76239724292659 21.596729820546184 30.640803209886418 21.000069726640806 3 22.721810185626673 20.15005070562335 36.231454507981134 20.89668460076884 4 21.90282706022051 20.12500517044401 36.167094175570526 21.805073593764963 5 29.381217078515277 19.48214126412349 30.69748174033594 20.4391599170253 6 29.194365062031075 19.968376917610797 30.854207746424716 19.983050273933415 7 30.115054084289255 24.583424336853504 25.016170428316396 20.28535115054084 8 26.8886229706215 22.007911922947958 30.03761137036433 21.065853736066206 9 23.38370570435699 20.510255455853297 34.361093764900225 21.744945074889486 10-11 28.20902509994844 21.336562199182797 28.12130257035984 22.333110130508924 12-13 27.305120393402564 25.671170836878954 23.81368800677683 23.210020762941657 14-15 27.12461113790407 23.17749728017956 25.329641160584217 24.36825042133216 16-17 26.74132031495934 22.767258112643386 24.83942364779023 25.651997924607045 18-19 26.482311479158398 23.52333330257158 25.1436214991843 24.850733719085717 20-21 25.442337729048404 24.040284058584323 25.471417991161438 25.045960221205842 22-23 25.5844963839559 23.8471776604046 25.169838203431578 25.398487752207927 24-25 25.719784011476182 24.100915982474003 24.749258385397603 25.43004162065221 26-27 25.62107723558479 23.994416229080727 24.880977649535446 25.503528885799042 28-29 25.418357101641842 24.511717510816677 24.678200303172794 25.391725084368687 30-31 25.596269560707004 24.298091944583486 24.554663082441504 25.550975412268013 32-33 25.435242920438828 24.460884892595338 24.631269117147934 25.4726030698179 34-35 25.222891973950944 24.753749601378946 24.49254130021728 25.530817124452827 36-37 25.46813954307499 24.55625213763665 24.449200473262696 25.526407846025663 38-39 25.390844153232333 24.160633807274223 24.620690526778414 25.827831512715026 40-41 25.344945790497526 24.523487229674114 24.423838502205182 25.70772847762318 42-43 25.49829592573082 24.501519503607458 24.213256018733922 25.7869285519278 44-45 25.723337537539397 24.19324271180443 24.329330608892274 25.7540891417639 46-47 25.410520756121297 24.377485051014457 24.51842312509356 25.693571067770694 48-49 25.601511483854928 24.149556405380686 24.440834930761042 25.808097180003347 50-51 25.50622552817509 24.52495812740559 24.873674662592794 25.095141681826526 52-53 25.612318097248444 24.06949508451797 24.96393175191977 25.35425506631382 54-55 25.31323730596446 24.377056214473665 24.599619411651645 25.71008706791023 56-57 25.50240836770678 24.419754311985002 24.852310350625338 25.225526969682875 58-59 25.428701239196993 24.497768953129683 24.66287898503328 25.41065082264004 60-61 25.701889551723223 24.666691569208552 24.491359386263266 25.140059492804955 62-63 25.502849434623904 24.768421827947734 24.803362463861156 24.925366273567207 64-65 25.68102997742755 24.768892296384244 24.38744623676934 25.162631489418867 66-67 26.07306935002721 24.519192604184422 24.53178470265417 24.875953343134196 68-69 25.756944482191475 24.31223343260603 24.899072701601536 25.03174938360096 70-71 25.74883653388959 24.46770730017459 24.55076422575465 25.23269194018117 72-73 25.499342660488217 24.213646263656372 24.844100440697968 25.442910635157446 74-75 25.21456740478975 24.20753850439309 25.041324301694427 25.53656978912273 76-77 25.172212224738864 24.28451177911538 24.708327857333813 25.834948138811942 78-79 25.582937202613564 24.42535699061291 24.619659778371876 25.372046028401645 80-81 25.399770444260135 24.26801358097982 24.916582631068486 25.415633343691557 82-83 25.426719606261 24.873316375190253 24.682401399390315 25.017562619158433 84-85 25.607587349730643 24.687184927254417 24.570394822338677 25.134832900676262 86-87 25.35327510134423 24.64626211327904 24.861535512649922 25.138927272726807 88-89 25.425645738055756 25.43022752265445 24.184915390822123 24.959211348467665 90-91 25.483111488284244 24.714669372358 24.3778288519295 25.424390287428256 92-93 25.47114778986277 24.912758196342484 24.439073938584016 25.177020075210727 94-95 25.413325376008107 25.14634008811061 23.868024201941076 25.572310333940212 96-97 25.37431090906886 24.59407903291454 24.37000981290051 25.661600245116094 98-99 25.434949125753008 24.735865892204398 24.365576728347083 25.46360825369551 100-101 25.644465364782715 25.349596242299516 23.664027435206094 25.341910957711676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2829.0 1 1876.0 2 1289.0 3 1529.0 4 1570.0 5 1570.0 6 1454.5 7 1601.0 8 1774.0 9 1907.0 10 2150.5 11 2472.5 12 2776.5 13 3113.5 14 3504.0 15 3944.0 16 4403.5 17 5048.5 18 5837.0 19 6618.5 20 7540.5 21 8582.0 22 9674.0 23 11120.5 24 12901.0 25 14674.0 26 16869.5 27 19309.5 28 21778.0 29 24791.5 30 28409.5 31 32908.5 32 39158.5 33 47168.5 34 56289.5 35 68876.5 36 84175.0 37 103597.0 38 126673.5 39 149248.5 40 175367.0 41 213210.5 42 271808.5 43 361195.5 44 499019.0 45 679423.0 46 867923.0 47 1047639.0 48 1175551.5 49 1206188.0 50 1162573.0 51 1131005.5 52 1112025.0 53 1146668.5 54 1179429.0 55 1141202.5 56 1091369.0 57 969961.5 58 816164.5 59 685084.5 60 561535.5 61 407035.5 62 248696.0 63 151248.5 64 97777.0 65 58984.5 66 31147.5 67 19106.5 68 13835.0 69 10496.0 70 7904.5 71 5990.5 72 4573.5 73 3541.5 74 2739.0 75 2042.0 76 1559.0 77 1169.0 78 855.0 79 595.0 80 439.0 81 309.5 82 217.0 83 149.0 84 96.5 85 73.0 86 41.0 87 30.5 88 32.0 89 24.5 90 21.0 91 16.5 92 13.5 93 12.5 94 9.0 95 8.0 96 5.5 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15458806423464244 2 0.0 3 0.0 4 0.09734044134912848 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.9992709464468637E-4 12-13 1.9226095810556817E-4 14-15 0.0012073988169029682 16-17 0.0 18-19 0.0 20-21 5.203863266057379E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1022961598052576E-4 30-31 6.665046547659697E-5 32-33 0.0 34-35 0.0 36-37 7.126472847113061E-4 38-39 0.0026378203452083956 40-41 0.0011586927075162242 42-43 7.690438324222728E-5 44-45 3.409427657072076E-4 46-47 0.0076571130914844295 48-49 0.003729862587248023 50-51 0.004783452637666536 52-53 0.0020328391970362075 54-55 0.007769906186906363 56-57 0.012955825096873886 58-59 0.019508078549111654 60-61 0.010487194394798392 62-63 0.00851331522491456 64-65 0.010164195985181038 66-67 0.02264065042651171 68-69 0.018323751047181352 70-71 0.012576430139545566 72-73 0.017441914119337147 74-75 0.021061547090604642 76-77 0.03474796382827969 78-79 6.690681342073773E-4 80-81 5.588385182268515E-4 82-83 0.0012612318851725273 84-85 0.009246470345157127 86-87 0.01671132247853599 88-89 0.01848268677254862 90-91 0.02110768972054998 92-93 0.0215204099106166 94-95 0.02935440308355815 96-97 0.027729157117705745 98-99 0.024837552307798 100-101 0.29985275374088555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.950474E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.41258172500495 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4105713598134 16.78972767346337 2 9.210758684384883 3.9445224615738073 3 3.143608223592737 2.0193830399723134 4 1.458553001860234 1.2492554141033423 5 1.003485511479096 1.0743607762202267 6 0.7545968238235001 0.9694719695729915 7 0.5300219360288417 0.7944396614884044 8 0.4229407271122475 0.7245002307299213 9 0.3396394659938005 0.6545302040366364 >10 3.384246441036905 16.041716322194045 >50 0.7019770219695302 10.54834830959592 >100 0.5337867053802516 22.324654728138363 >500 0.06780174264667642 10.0570819793934 >1k 0.03780954315958379 12.494313019387821 >5k 1.7895340338122703E-4 0.24178952259960054 >10k+ 2.385831489180143E-5 0.0719046875299053 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1279309542193334E-4 0.0 0.0 2 1.1279309542193334E-4 0.0 0.0 3 1.1792005430474849E-4 0.0 0.0 4 1.1792005430474849E-4 0.0 0.0 5 1.3330093095319394E-4 0.0 0.0 6 1.3330093095319394E-4 0.0 0.0 7 1.384278898360091E-4 0.0 5.126958882815151E-6 8 1.4355484871882425E-4 0.0 5.126958882815151E-6 9 1.486818076016394E-4 0.0 1.0253917765630303E-5 10-11 1.7688008145712273E-4 0.0 1.0253917765630303E-5 12-13 2.3584010860949696E-4 0.0 1.0253917765630303E-5 14-15 3.358158068243924E-4 0.0 1.0253917765630303E-5 16-17 4.2297410783225E-4 0.0 1.0253917765630303E-5 18-19 6.024176687307802E-4 0.0 1.0253917765630303E-5 20-21 8.510751745473152E-4 0.0 1.0253917765630303E-5 22-23 0.0010715344065083668 0.0 1.0253917765630303E-5 24-25 0.0013817154189186833 0.0 1.0253917765630303E-5 26-27 0.0016329364041766257 0.0 1.5380876648445454E-5 28-29 0.001950807854911165 0.0 2.0507835531260606E-5 30-31 0.0023532741272121547 0.0 2.0507835531260606E-5 32-33 0.0027685577967201816 0.0 2.3071314972668183E-5 34-35 0.0033453406710368862 0.0 3.076175329689091E-5 36-37 0.003960575736974704 0.0 3.076175329689091E-5 38-39 0.0045988821178851915 0.0 3.076175329689091E-5 40-41 0.00557300430562007 0.0 3.076175329689091E-5 42-43 0.006577888246651839 0.0 3.3325232738298486E-5 44-45 0.007716073118636803 0.0 3.588871217970606E-5 46-47 0.008990122401016369 0.0 4.101567106252121E-5 48-49 0.010397472614349128 0.0 4.101567106252121E-5 50-51 0.01217140038780317 0.0 4.101567106252121E-5 52-53 0.0144144448990348 0.0 4.101567106252121E-5 54-55 0.017154804421899497 0.0 4.101567106252121E-5 56-57 0.020251487587119848 0.0 4.101567106252121E-5 58-59 0.02381216053123497 0.0 4.101567106252121E-5 60-61 0.02837002697805764 0.0 4.101567106252121E-5 62-63 0.03421988706334973 0.0 4.870610938674394E-5 64-65 0.04117204330844708 0.0 5.1269588828151514E-5 66-67 0.04970586636889289 0.0 5.383306826955909E-5 68-69 0.0600956485449178 0.0 5.896002715237424E-5 70-71 0.07306941799788154 0.0 6.152350659378182E-5 72-73 0.08903733143840933 0.0 6.152350659378182E-5 74-75 0.10899914584865011 0.0 6.152350659378182E-5 76-77 0.13329836747375254 0.0 6.152350659378182E-5 78-79 0.16343206830749857 0.0 7.177742435941213E-5 80-81 0.20094346297361565 0.0 7.177742435941213E-5 82-83 0.24575308360942005 0.0 7.690438324222727E-5 84-85 0.3000039990279286 0.0 7.690438324222727E-5 86-87 0.3661879112461894 0.0 7.690438324222727E-5 88-89 0.44598646277776577 0.0 7.690438324222727E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 197390 0.0 63.998672 1 TAACTTT 21860 0.0 62.588894 1 TTTGTGT 208860 0.0 60.584103 2 AACTTTG 22835 0.0 60.1317 2 ACTTTGT 61530 0.0 59.35842 1 TTGTGTT 238955 0.0 54.602783 3 TGTGTTT 252010 0.0 52.09101 4 GTGTTTG 305320 0.0 43.075367 5 TTTGAGG 86550 0.0 35.66702 8 GTTTGAG 204225 0.0 34.872105 7 TTGAGGG 41825 0.0 34.207764 9 TGTTTGA 384530 0.0 34.104816 6 TTGAGGT 24550 0.0 32.611263 9 GTTTGAC 76470 0.0 30.88027 7 TTTGAGC 45880 0.0 30.29375 8 TTTGAGA 25915 0.0 29.518755 8 TTGAGTC 22225 0.0 29.362005 4 TTGAGGC 15540 0.0 28.295767 9 GTTTGAA 26235 0.0 28.018417 7 TTTGACA 9475 0.0 27.964605 8 >>END_MODULE