FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30304.34100000035cbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30304.34100000035cbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19504740
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1973900.063.9986721
TAACTTT218600.062.5888941
TTTGTGT2088600.060.5841032
AACTTTG228350.060.13172
ACTTTGT615300.059.358421
TTGTGTT2389550.054.6027833
TGTGTTT2520100.052.091014
GTGTTTG3053200.043.0753675
TTTGAGG865500.035.667028
GTTTGAG2042250.034.8721057
TTGAGGG418250.034.2077649
TGTTTGA3845300.034.1048166
TTGAGGT245500.032.6112639
GTTTGAC764700.030.880277
TTTGAGC458800.030.293758
TTTGAGA259150.029.5187558
TTGAGTC222250.029.3620054
TTGAGGC155400.028.2957679
GTTTGAA262350.028.0184177
TTTGACA94750.027.9646058
Produced by FastQC (version 0.11.2)