FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30301.34100000035c7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30301.34100000035c7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14174106
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195400.13785701898941632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1085300.063.7141571
TTTGTGT1140500.060.7716262
ACTTTGT341600.056.281521
TTGTGTT1313100.054.238673
TGTGTTT1404000.051.0656054
GTGTTTG1528300.046.9127585
AACTTTG150650.044.6443372
TAACTTT156800.042.6217081
TTTGAGG452350.033.2135128
GTTTGAG1181400.032.5808647
TTGAGGG208550.031.8770739
TGTTTGA2268750.031.5852496
TTTGAGT452050.029.0938873
TTTGAGC320650.027.1150288
TTGAGTC165250.025.9731944
GTTTGAC455950.025.0363037
TTGAGGC107150.024.9946779
TTGAGGT160900.024.0526089
TTGAGCC183150.022.9973269
GTTTGAA247300.022.31452
Produced by FastQC (version 0.11.2)