FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30295.34100000035c39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30295.34100000035c39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524697
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT557480.4121940772499376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA388170.28700827826309155No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC216890.16036588472185367No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC167890.12413586788672605No Hit
CCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA145290.10742569685664677No Hit
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT144290.1066863087579707No Hit
TTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACT135610.10026842006146236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT260700.060.0324971
CTTTGTG721200.057.3468671
TTTGTGT812750.051.0206872
TTGTGTT858850.049.6710553
TGTGTTT948150.045.2886054
GTGTTTG1055650.040.6932645
TTTGAGG410750.030.1141978
GTTTGAG785900.029.5207427
TGTTTGA1467300.029.3741441
GTTTGAA97050.029.2848832
TTGAGTC67550.028.7064
TTGAGGG310050.028.3786149
GTTTGAC260900.027.6273217
TTTGAGT249150.026.8011423
TGATTTT91450.025.2861775
TTTGACG125600.024.4221558
TTGAGGC42300.024.1349099
TTTGAGA105800.023.9879673
TTTGACC66900.023.9191048
TTGAGTG107100.023.0755334