FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30292.34100000035c02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30292.34100000035c02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15405556
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT899120.5836335929712632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA592400.3845365918633511No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC193130.12536386223256077No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174780.11345257516184422No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC173990.11293977315716486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT346700.057.243021
CTTTGTG960500.047.3911
TTGTGTT1060750.044.3124543
TTTGTGT1042450.043.799972
TGTGTTT1151200.041.0579034
GTGTTTG1198300.039.4001925
CCGATCT7650.031.656048
TTGAGGG374250.029.5762029
AGTTTCG8050.029.4932087
TTCCGAT8450.029.220966
TTTGACG97850.028.922338
TTTGAGG525000.028.3547698
GTTTGAG1000200.028.0479747
GTTTGAC235300.027.8688857
TTGAGTC87900.027.848694
TGTTTGA1747150.027.0529926
TTGACGG77000.026.6412739
CTTCCGA9550.026.3523245
TTTGATT262100.024.8994643
GTTTGAT476800.024.3940642
Produced by FastQC (version 0.11.2)