##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l06n01_30291.34100000035bb0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12164951 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73753466002452 33.0 33.0 33.0 33.0 33.0 2 32.76881181025719 33.0 33.0 33.0 33.0 33.0 3 32.78838862565085 33.0 33.0 33.0 33.0 33.0 4 36.70187508359056 37.0 37.0 37.0 37.0 37.0 5 36.7258302150169 37.0 37.0 37.0 37.0 37.0 6 36.68066710667392 37.0 37.0 37.0 37.0 37.0 7 36.644728449789895 37.0 37.0 37.0 37.0 37.0 8 36.63563568813389 37.0 37.0 37.0 37.0 37.0 9 36.626140869782375 37.0 37.0 37.0 37.0 37.0 10-11 36.614585870506176 37.0 37.0 37.0 37.0 37.0 12-13 36.5718960972387 37.0 37.0 37.0 37.0 37.0 14-15 38.68113484386414 40.0 37.0 40.0 37.0 40.0 16-17 38.629037593328576 40.0 37.0 40.0 37.0 40.0 18-19 38.58869558948491 40.0 37.0 40.0 37.0 40.0 20-21 38.5505967101717 40.0 37.0 40.0 37.0 40.0 22-23 38.50005819998782 40.0 37.0 40.0 37.0 40.0 24-25 38.47103584716453 40.0 37.0 40.0 37.0 40.0 26-27 38.38321342190363 40.0 37.0 40.0 37.0 40.0 28-29 38.326401273626175 40.0 37.0 40.0 37.0 40.0 30-31 38.26544956901183 40.0 37.0 40.0 37.0 40.0 32-33 38.17215350887973 40.0 37.0 40.0 37.0 40.0 34-35 38.08321673469955 40.0 37.0 40.0 37.0 40.0 36-37 38.009981256809006 40.0 37.0 40.0 37.0 40.0 38-39 37.92359406133243 40.0 37.0 40.0 35.0 40.0 40-41 37.794129873601634 40.0 37.0 40.0 33.0 40.0 42-43 37.669848115294506 40.0 37.0 40.0 33.0 40.0 44-45 37.5622730416259 40.0 37.0 40.0 33.0 40.0 46-47 37.41150182191445 40.0 37.0 40.0 33.0 40.0 48-49 37.238605441156324 40.0 37.0 40.0 33.0 40.0 50-51 37.0671551410277 37.0 37.0 40.0 33.0 40.0 52-53 36.85524076504706 37.0 37.0 40.0 33.0 40.0 54-55 36.66370781107133 37.0 37.0 40.0 33.0 40.0 56-57 36.44095315303777 37.0 37.0 40.0 33.0 40.0 58-59 36.205264698559 37.0 37.0 40.0 33.0 40.0 60-61 35.9333199122627 37.0 37.0 40.0 33.0 40.0 62-63 35.67036381815266 37.0 35.0 38.5 33.0 40.0 64-65 35.40522851263437 37.0 33.0 37.0 33.0 40.0 66-67 35.122437690049054 37.0 33.0 37.0 30.0 40.0 68-69 34.889676662076155 37.0 33.0 37.0 27.0 40.0 70-71 34.612436581125564 37.0 33.0 37.0 27.0 37.0 72-73 34.34858993677821 37.0 33.0 37.0 27.0 37.0 74-75 34.02558584083076 37.0 33.0 37.0 27.0 37.0 76-77 31.216080072990017 33.0 30.0 35.0 24.5 37.0 78-79 33.71006443018143 35.0 33.0 37.0 27.0 37.0 80-81 34.1234164445052 37.0 33.0 37.0 27.0 37.0 82-83 34.077619219345806 37.0 33.0 37.0 27.0 37.0 84-85 33.97466985275979 37.0 33.0 37.0 27.0 37.0 86-87 33.89903839316739 37.0 33.0 37.0 27.0 37.0 88-89 33.74703210888396 37.0 33.0 37.0 27.0 37.0 90-91 33.63285799507125 37.0 33.0 37.0 27.0 37.0 92-93 33.50367777889117 37.0 33.0 37.0 27.0 37.0 94-95 33.39186384721155 37.0 33.0 37.0 27.0 37.0 96-97 33.30855175659976 37.0 33.0 37.0 27.0 37.0 98-99 33.16867268926936 37.0 33.0 37.0 27.0 37.0 100-101 31.30683148662087 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1163644614996997 2306 2 0.10594306619569238 2306 3 0.10373617276248126 2306 4 0.05461395367330368 2306 5 0.07044242093810027 2306 6 0.07307385303528235 2306 7 0.11877796603835122 2306 8 0.1210320914462244 2306 9 0.09248560376303772 2306 10-11 0.11271889234370747 2306 12-13 0.1279997521064402 2306 14-15 0.2584377233466384 2306 16-17 0.26841468071148 2306 18-19 0.24445560284402035 2306 20-21 0.22314517889321195 2306 22-23 0.22790887315273523 2306 24-25 0.24136968624007693 2306 26-27 0.2565586663830146 2306 28-29 0.20244337885011987 2306 30-31 0.23098851286418665 2306 32-33 0.27347482835774173 2306 34-35 0.26862743936203515 2306 36-37 0.24355196790613576 2306 38-39 0.25127961103521557 2306 40-41 0.28206874557024975 2306 42-43 0.304475599927315 2306 44-45 0.19946500555474955 2306 46-47 0.2708053402560111 2306 48-49 0.24852654272296348 2306 50-51 0.3011470952629338 2306 52-53 0.30521945929303484 2306 54-55 0.2206394356451895 2306 56-57 0.27762938628210065 2306 58-59 0.16395737636605645 2306 60-61 0.1522216498380331 2306 62-63 0.11028697655002162 2306 64-65 0.2702744133413191 2306 66-67 0.3103571699595804 2306 68-69 0.20622642899966337 2306 70-71 0.20585006444903797 2306 72-73 0.12935739151900094 2306 74-75 0.035888911607941054 2306 76-77 -0.2100266775980728 2306 78-79 0.15725075556272827 2306 80-81 0.3184701210705896 2306 82-83 0.22788875842564948 2306 84-85 0.2546640650272991 2306 86-87 0.2225264997077332 2306 88-89 0.1522215005929155 2306 90-91 0.03181164817161175 2306 92-93 0.22823278941377367 2306 94-95 0.2169927202811266 2306 96-97 0.1936134777875651 2306 98-99 0.26765000656434523 2306 100-101 0.35787776679451966 2307 1 0.11384452911884324 2307 2 0.08644289241903635 2307 3 0.08495827573202064 2307 4 0.05180668503918895 2307 5 0.06766742023082628 2307 6 0.07211412480202029 2307 7 0.09527867406035284 2307 8 0.10720661733252257 2307 9 0.09055519998207018 2307 10-11 0.07410321783948604 2307 12-13 0.10632866847401345 2307 14-15 0.19677369465018302 2307 16-17 0.18234060771052185 2307 18-19 0.21113046746838648 2307 20-21 0.16280509872498072 2307 22-23 0.16080312715202894 2307 24-25 0.12917319574954433 2307 26-27 0.1558958140103499 2307 28-29 0.15252134942302575 2307 30-31 0.09808689231996937 2307 32-33 0.10079556676708279 2307 34-35 0.05408596851584946 2307 36-37 0.034376546690786824 2307 38-39 0.10015088961456797 2307 40-41 0.11296827860783054 2307 42-43 0.10368628625559495 2307 44-45 0.0549310912256189 2307 46-47 0.09192988502334742 2307 48-49 -0.007567045471866152 2307 50-51 -0.105740248397872 2307 52-53 -0.012940652492574145 2307 54-55 -0.0062230326011061265 2307 56-57 -0.04176489511964121 2307 58-59 -0.10681213920366162 2307 60-61 -0.0907553472712479 2307 62-63 -0.04909835702805054 2307 64-65 -0.08735557781746195 2307 66-67 -0.13364954439328613 2307 68-69 -0.3197541244151054 2307 70-71 -0.3256458648857006 2307 72-73 -0.3119857838281277 2307 74-75 -0.32029808410320015 2307 76-77 -0.3017821197868855 2307 78-79 -0.10317254332915837 2307 80-81 0.020375076787274793 2307 82-83 -0.09762151148099463 2307 84-85 -0.15779355513041082 2307 86-87 -0.11025979819069676 2307 88-89 -0.03041912012118786 2307 90-91 -0.15796575370129062 2307 92-93 -0.03730097826781531 2307 94-95 -0.20207379770614864 2307 96-97 -0.2085521975845097 2307 98-99 -0.15209309919012526 2307 100-101 -0.11187932719507643 2201 1 0.10810438545368584 2201 2 0.08090848051986654 2201 3 0.09187979127739965 2201 4 0.07331684476555012 2201 5 0.08514145542184792 2201 6 0.07982218436489319 2201 7 0.07350294203106245 2201 8 0.08397436663418034 2201 9 0.02617575831821739 2201 10-11 0.06642334316951093 2201 12-13 0.08682454306740084 2201 14-15 0.13683208704613037 2201 16-17 0.14511608362231954 2201 18-19 0.132974477550313 2201 20-21 0.1307703648837233 2201 22-23 0.07979005079789658 2201 24-25 0.1314900418181395 2201 26-27 0.13787238284970726 2201 28-29 0.09822049035227565 2201 30-31 0.06532964383573159 2201 32-33 0.11621395983323879 2201 34-35 0.19731722586128342 2201 36-37 0.16157688323396968 2201 38-39 0.12053024271443036 2201 40-41 0.10913220098747445 2201 42-43 0.11671530652238715 2201 44-45 0.052072688297741365 2201 46-47 0.05806935655959933 2201 48-49 -0.013357874071807885 2201 50-51 -0.01400332894083789 2201 52-53 -0.010253991905706528 2201 54-55 0.004153496025409709 2201 56-57 0.06674359146531117 2201 58-59 -0.01605169762837022 2201 60-61 0.0076129808080835915 2201 62-63 -0.05821637932223922 2201 64-65 -0.13840482179211477 2201 66-67 -0.018854091316633514 2201 68-69 -0.12699596635470556 2201 70-71 -0.3308014861860613 2201 72-73 -0.3533014857456678 2201 74-75 -0.2965084786438297 2201 76-77 -0.7259895917156953 2201 78-79 -0.11212785920341872 2201 80-81 0.05035261264970359 2201 82-83 0.01875953630286631 2201 84-85 -0.006200959080366886 2201 86-87 -0.07925581759818101 2201 88-89 -0.11180356684754855 2201 90-91 -0.11898850028619279 2201 92-93 -0.010419113062219765 2201 94-95 -0.14500244145628471 2201 96-97 -0.2698694792922183 2201 98-99 -0.21784007664460603 2201 100-101 -0.29186613834073327 1101 1 0.10494857416625791 1101 2 0.08665125451392441 1101 3 0.06891530794415957 1101 4 0.04735976952786558 1101 5 0.06389574315633695 1101 6 0.05587935243763553 1101 7 0.03431796802036757 1101 8 0.04403942213845369 1101 9 0.05011917086778794 1101 10-11 0.07328170229214948 1101 12-13 0.060417404896703886 1101 14-15 0.09721182371455228 1101 16-17 0.08562910214222796 1101 18-19 0.07667909627176783 1101 20-21 0.08648860758513166 1101 22-23 0.08842425938710363 1101 24-25 0.0013076973813710424 1101 26-27 0.04458939269641604 1101 28-29 -0.012753851947792327 1101 30-31 0.05191118804592065 1101 32-33 0.03934256573972306 1101 34-35 0.04618989925928929 1101 36-37 -0.08055686182509447 1101 38-39 -0.10489364382938504 1101 40-41 -0.09495862188982329 1101 42-43 -0.17686838147440653 1101 44-45 -0.14111375726795217 1101 46-47 -0.08196974512736688 1101 48-49 -0.105953600949654 1101 50-51 -0.17607748279671398 1101 52-53 -0.12043118815798692 1101 54-55 -0.17598507594046708 1101 56-57 -0.12128233138547273 1101 58-59 -0.17373418560787002 1101 60-61 -0.18926735164335184 1101 62-63 -0.209141439690832 1101 64-65 -0.26045540560785696 1101 66-67 -0.178155628481953 1101 68-69 -0.3468592330261444 1101 70-71 -0.3374060649736066 1101 72-73 -0.34725165931947544 1101 74-75 -0.3964575129076451 1101 76-77 -0.7395563965059537 1101 78-79 -0.24890123457380753 1101 80-81 -0.10716301092898561 1101 82-83 -0.10021860391226056 1101 84-85 -0.13718928792678753 1101 86-87 -0.2706557044413458 1101 88-89 -0.1924242972575172 1101 90-91 -0.26688342184089464 1101 92-93 -0.18967994537784705 1101 94-95 -0.223765277241462 1101 96-97 -0.22069147920061027 1101 98-99 -0.29716755149882346 1101 100-101 -0.4018794539457353 2304 1 0.09916967239066565 2304 2 0.09227992782227545 2304 3 0.07886825724769153 2304 4 0.03252505157987429 2304 5 0.05033599755753215 2304 6 0.04406537861176929 2304 7 0.05072426553564213 2304 8 0.034379290022151565 2304 9 0.1082748666485216 2304 10-11 0.0777792453673456 2304 12-13 0.06322635493221895 2304 14-15 0.09679981453324871 2304 16-17 0.111620730889193 2304 18-19 0.11177599064886579 2304 20-21 0.10356177147484402 2304 22-23 0.10429951986220232 2304 24-25 0.06541236884209667 2304 26-27 0.11393507997852481 2304 28-29 0.05427069509725868 2304 30-31 0.07000415374268698 2304 32-33 0.04287741113421362 2304 34-35 0.07340238050535675 2304 36-37 0.012857117993199552 2304 38-39 -0.08948746681919317 2304 40-41 -0.08941297114719049 2304 42-43 -0.008802029553393709 2304 44-45 0.011550416573065547 2304 46-47 -0.05731666873544583 2304 48-49 0.014261835744562745 2304 50-51 -0.09532145997512487 2304 52-53 -0.023345190686619333 2304 54-55 -0.030552967360080174 2304 56-57 -0.05436221908834682 2304 58-59 -0.13539878446951548 2304 60-61 -0.05900411042448184 2304 62-63 -0.011951520767048862 2304 64-65 0.013259474011512395 2304 66-67 -0.017581331969871883 2304 68-69 -0.2109480699170021 2304 70-71 -0.2201228438460774 2304 72-73 -0.28705719552200293 2304 74-75 -0.33703766277918845 2304 76-77 -0.7052618994099262 2304 78-79 -0.2539993334047992 2304 80-81 -0.06425331112986044 2304 82-83 -0.04195660821093128 2304 84-85 -0.08705714715799218 2304 86-87 -0.08818440019742724 2304 88-89 -0.14954514152598364 2304 90-91 -0.19485430318302122 2304 92-93 -0.12298265406813869 2304 94-95 -0.17737867177155664 2304 96-97 -0.24692271548897793 2304 98-99 -0.2302248333089878 2304 100-101 -0.2675403329666963 2202 1 0.07735895685184602 2202 2 0.05559279015451324 2202 3 0.044046028593960784 2202 4 0.028426631146871273 2202 5 6.0314082706725E-4 2202 6 0.06770170704602663 2202 7 0.028185859178897488 2202 8 0.03382059287487493 2202 9 0.021355073006631642 2202 10-11 0.04229240699422121 2202 12-13 0.06049621868519495 2202 14-15 0.07561870684840954 2202 16-17 0.060385181698997314 2202 18-19 0.057859957514111215 2202 20-21 0.03591128214839756 2202 22-23 0.0041781368727882295 2202 24-25 -0.004521487812624514 2202 26-27 0.02589375512145864 2202 28-29 -0.01820211098915081 2202 30-31 -0.048723349090593615 2202 32-33 -0.018315511669996454 2202 34-35 -0.030296828011955768 2202 36-37 -0.10162271134429801 2202 38-39 -0.05744773838776496 2202 40-41 0.019151903875602727 2202 42-43 -0.06563754403416056 2202 44-45 -0.05549267655380419 2202 46-47 -0.06376539687020255 2202 48-49 -0.08102889185962425 2202 50-51 -0.11189523195012896 2202 52-53 -0.14131571108273278 2202 54-55 -0.09746689046170331 2202 56-57 -0.022730148554202856 2202 58-59 -0.12502973635071157 2202 60-61 -0.14389144513922503 2202 62-63 -0.1306214121217053 2202 64-65 -0.11790259421335492 2202 66-67 -0.09304987016091815 2202 68-69 -0.16196603188961234 2202 70-71 -0.23600444514050878 2202 72-73 -0.16894459457650868 2202 74-75 -0.21433419051270164 2202 76-77 -0.24726783058800095 2202 78-79 -0.1359586704550324 2202 80-81 -0.01435668972240478 2202 82-83 -0.06914909655517931 2202 84-85 -0.12182194017535863 2202 86-87 -0.08158143063250378 2202 88-89 -0.09380079307783973 2202 90-91 -0.09467776125183036 2202 92-93 0.01876534982600475 2202 94-95 -0.04567479651161932 2202 96-97 -0.24018714234722438 2202 98-99 -0.15193465610958157 2202 100-101 -0.09947771934945493 1102 1 0.08624892174709231 1102 2 0.06073581901340219 1102 3 0.062328839822669124 1102 4 -0.017699799101428937 1102 5 0.019525592557563698 1102 6 0.015130070892958258 1102 7 0.032579524832357265 1102 8 0.02209677342344918 1102 9 0.04071314101475565 1102 10-11 0.03021715772395339 1102 12-13 0.05432344653868171 1102 14-15 0.06536381723613971 1102 16-17 0.07280286866966179 1102 18-19 0.04878119241982404 1102 20-21 0.04226735978667051 1102 22-23 0.09167343808372408 1102 24-25 0.06999583772802254 1102 26-27 0.030537532820652302 1102 28-29 0.045075302225420444 1102 30-31 0.0666002313916465 1102 32-33 0.03825679427160367 1102 34-35 0.0853331616922901 1102 36-37 0.005231352213748153 1102 38-39 -0.043178707953458684 1102 40-41 0.012214673079832039 1102 42-43 -0.03605347434434236 1102 44-45 -0.03903370096406178 1102 46-47 -0.035020851588690505 1102 48-49 -0.05042809130906534 1102 50-51 -0.10208842671488583 1102 52-53 -0.03265226870914972 1102 54-55 -0.05477820109974374 1102 56-57 0.021187782258024868 1102 58-59 -0.06640837606279604 1102 60-61 -0.12475876299413358 1102 62-63 -0.11052947066795582 1102 64-65 -0.0885601416318238 1102 66-67 -0.12891376449604053 1102 68-69 -0.13716175319106583 1102 70-71 -0.17252338487357122 1102 72-73 -0.2506320587816191 1102 74-75 -0.2892357169578048 1102 76-77 -0.4668728801159716 1102 78-79 -0.1280389157085935 1102 80-81 -0.018250487025966322 1102 82-83 -0.11548428281923862 1102 84-85 -0.10632000800998043 1102 86-87 -0.1026920788128578 1102 88-89 -0.13741958808321897 1102 90-91 -0.2662865299337085 1102 92-93 -0.15024495378734315 1102 94-95 -0.30344530695089134 1102 96-97 -0.3125729329279352 1102 98-99 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20-21 -0.4278965855743593 1213 22-23 -0.4102880584341335 1213 24-25 -0.37914668058570555 1213 26-27 -0.4036553421369433 1213 28-29 -0.3963010718186979 1213 30-31 -0.32903027504978866 1213 32-33 -0.3367410225160299 1213 34-35 -0.277599856503457 1213 36-37 -0.27382847745261785 1213 38-39 -0.313797291165514 1213 40-41 -0.25999780758090907 1213 42-43 -0.22832548977378053 1213 44-45 -0.20982748645082694 1213 46-47 -0.3118860632587399 1213 48-49 -0.2103412755184948 1213 50-51 -0.13931150387519153 1213 52-53 -0.11395469510052436 1213 54-55 -0.6059249325146538 1213 56-57 -1.5731370761241052 1213 58-59 -0.4583753875177763 1213 60-61 -0.253633466249795 1213 62-63 -0.2036025661594394 1213 64-65 -1.6231178048564985 1213 66-67 -0.6142207098651227 1213 68-69 -0.18904855519148356 1213 70-71 -0.05641531744866768 1213 72-73 -0.9308917312440173 1213 74-75 -0.32373578873495035 1213 76-77 -0.40776862065729347 1213 78-79 -0.40486844243404363 1213 80-81 -0.3372971590365239 1213 82-83 -1.2495898751287768 1213 84-85 -0.5063967444193196 1213 86-87 -0.35685521545496357 1213 88-89 -1.1888983043439083 1213 90-91 -1.3636629888042506 1213 92-93 -2.5267509758710958 1213 94-95 -1.1246264571081852 1213 96-97 -0.40422360794501344 1213 98-99 -0.06987289911923966 1213 100-101 0.05413298330606153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 32.0 8 319.0 9 976.0 10 731.0 11 245.0 12 230.0 13 505.0 14 1297.0 15 3042.0 16 5783.0 17 9146.0 18 12705.0 19 16203.0 20 20292.0 21 24614.0 22 29960.0 23 35681.0 24 43272.0 25 53182.0 26 66037.0 27 81886.0 28 102577.0 29 130613.0 30 167671.0 31 219822.0 32 295671.0 33 414353.0 34 637427.0 35 1199755.0 36 2757097.0 37 4660632.0 38 1173150.0 39 44.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.936811591100604 25.398471869631816 31.095991631961066 23.568724907306514 2 23.819767132641964 24.18687917444139 32.131703613109494 19.861650079807145 3 21.443547121562595 22.763979896014376 36.30417418039744 19.48829880202559 4 20.914658310024745 22.57089837411486 36.345517213686165 20.168926102174233 5 26.12438800616624 21.93370939184219 32.77333381778521 19.168568784206364 6 26.655853633254033 22.139698242877962 32.4089562562079 18.79549186766011 7 26.809125659445733 25.50124533999356 28.920363098873146 18.76926590168756 8 25.363554690849142 23.917860417193623 31.346275048703443 19.372309843253788 9 21.985719465701095 22.715151092676 35.387343524852675 19.911785916770235 10-11 25.596310802789297 23.508808892584028 30.672019050182232 20.22286125444444 12-13 25.395128612109325 26.842230883092107 27.087982369650003 20.67465813514856 14-15 25.609089439873973 24.552738154797147 28.04319068841094 21.794981716917945 16-17 24.960556766730914 24.50185783732298 28.220569897897658 22.317015498048452 18-19 24.295671227940005 24.96671379934042 28.869783363697888 21.86783160902169 20-21 23.0927574372092 25.90799711201402 29.109229117692003 21.890016333084777 22-23 22.985957773278333 25.774456469245127 29.04929908883316 22.190286668643385 24-25 23.24578208329816 26.024371162695186 28.630637312061513 22.099209441945142 26-27 23.203373363361678 25.90354453544449 28.64840968122272 22.244672419971113 28-29 23.445101957983756 25.900712601277238 28.299935778543865 22.35424966219514 30-31 23.315278858467366 25.74184993132109 28.247899383996316 22.69497182621522 32-33 23.248408481053477 26.013787478469908 27.917613478262265 22.82019056221435 34-35 23.040754541469177 26.085353734675955 27.96948791655634 22.904403807298525 36-37 23.22712092070167 26.245176421169102 28.099958466409685 22.427744191719544 38-39 23.415246116701248 26.221559389559506 27.882194153860308 22.481000339878936 40-41 23.119861561328253 26.719712574196603 27.65585961933311 22.504566245142033 42-43 23.20457080777097 26.73706268740588 27.55780659657206 22.500559908251088 44-45 23.063003780136384 26.274455082057774 28.270142676823205 22.392398460982637 46-47 22.968910039055334 26.430626610949354 28.083480276221117 22.516983073774195 48-49 23.01962314002301 26.25069824279161 28.016254722258644 22.71342389492674 50-51 23.01017684617774 26.307798247967405 28.15438188799324 22.52764301786161 52-53 23.300612324376758 26.822972593885797 27.46624303698094 22.410172044756504 54-55 23.102820773436406 26.445836650673694 27.782840765237616 22.668501810652288 56-57 23.205708200216495 26.749285671328778 27.533745141703843 22.511260986750887 58-59 23.111436682751847 26.631273289236727 27.736873032842897 22.520416995168524 60-61 23.103680610303726 26.534850953642735 27.552954197911916 22.808514238141623 62-63 22.818783063182764 26.852283265605063 27.493202955476587 22.83573071573558 64-65 23.226171312075216 26.78248146066367 27.339567933555912 22.651779293705196 66-67 23.45584030791126 26.780003018367022 27.048264395795645 22.715892277926073 68-69 23.33642913538902 26.914135973184262 27.029015826822107 22.720419064604616 70-71 23.353865338688067 27.181591339758977 26.952244383973657 22.5122989375793 72-73 23.41915130658673 27.00389142168461 26.77361098041866 22.80334629131 74-75 23.45596360048244 26.95702782659186 26.87218408862952 22.71482448429618 76-77 23.360706222852084 26.714088028199708 27.149277006723466 22.775928742224743 78-79 23.622897213278662 26.639699120475523 26.906759127451352 22.83064453879446 80-81 23.592361507030912 26.68082846477852 26.87868892159413 22.848121106596434 82-83 23.415324764201596 26.79160651182768 27.06733677016553 22.725731953805195 84-85 23.035492646966638 27.32460899019904 26.8329762988427 22.806922063991625 86-87 22.901070605784284 27.045521197323634 26.987130647088485 23.066277549803598 88-89 22.669731065649586 27.612100103352965 26.790098522205962 22.92807030879149 90-91 22.719062044689398 27.464806305645762 26.82667412573889 22.98945752392595 92-93 22.679963587522753 27.858315948004797 26.50396846751496 22.957751996957487 94-95 22.697434324987913 28.01549483665104 26.332090912832275 22.954979925528775 96-97 22.53207897406486 28.103391107452047 26.37480920973516 22.98972070874793 98-99 22.92471376010787 27.893546473792824 26.157723846837538 23.02401591926176 100-101 23.233310475653973 28.061737914450386 25.814188875946787 22.890762733948858 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31618.0 1 17177.5 2 3569.0 3 4290.0 4 4557.5 5 3371.5 6 1506.5 7 1220.0 8 1372.0 9 1557.5 10 1581.5 11 1356.0 12 963.5 13 695.0 14 627.0 15 621.5 16 623.0 17 636.0 18 698.0 19 860.0 20 1042.5 21 1241.0 22 1548.5 23 1941.0 24 2546.0 25 3217.0 26 3689.0 27 4397.0 28 5643.5 29 7111.0 30 9495.0 31 13999.0 32 31308.5 33 58556.5 34 90208.0 35 160403.0 36 233791.0 37 335543.5 38 470102.5 39 510589.5 40 506244.0 41 521753.0 42 571650.5 43 673801.0 44 762249.0 45 802105.5 46 784610.0 47 702155.0 48 633678.5 49 607989.5 50 552861.0 51 485657.5 52 439330.0 53 385236.0 54 315477.0 55 264879.5 56 233297.5 57 189059.5 58 148324.5 59 119145.0 60 92169.5 61 75949.0 62 64461.5 63 48414.5 64 33443.5 65 25034.5 66 20612.0 67 16464.0 68 13187.0 69 10428.0 70 8008.5 71 6691.5 72 7535.5 73 7330.5 74 5046.5 75 3484.0 76 2800.5 77 2577.5 78 2593.5 79 2408.5 80 1772.5 81 1120.5 82 767.0 83 628.0 84 504.5 85 355.0 86 263.0 87 242.0 88 259.5 89 273.5 90 245.5 91 196.5 92 162.0 93 133.0 94 107.0 95 93.0 96 83.0 97 63.0 98 39.5 99 20.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1540162389474483 2 0.0 3 0.0 4 0.09916192839576583 5 0.0 6 1.6440674524706264E-5 7 0.0 8 0.0 9 0.0 10-11 2.301694433458877E-4 12-13 1.602965766158861E-4 14-15 0.0012577116011400294 16-17 0.0 18-19 0.0 20-21 5.918642828894255E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1097455304176729E-4 30-31 6.165252946764849E-5 32-33 0.0 34-35 0.0 36-37 8.097032203417835E-4 38-39 0.0026757197788959447 40-41 0.0011672878912541448 42-43 9.453387851706102E-5 44-45 3.7813551406824407E-4 46-47 0.007628472979463706 48-49 0.0039950839095036226 50-51 0.005117159945814825 52-53 0.0019605504370712222 54-55 0.007928515289539596 56-57 0.01321419214923266 58-59 0.019827453476795754 60-61 0.010706989284214955 62-63 0.008421735525280784 64-65 0.01002881146007082 66-67 0.022568935953790524 68-69 0.018446436816720427 70-71 0.012346946568054407 72-73 0.01785868270246218 74-75 0.02130711418401932 76-77 0.03499397572583728 78-79 6.740676555129568E-4 80-81 5.302117534217771E-4 82-83 0.001344025142394737 84-85 0.00898893879638315 86-87 0.01663796261900274 88-89 0.01859029271881161 90-91 0.02121258030550226 92-93 0.021672919192194034 94-95 0.029671307348463634 96-97 0.027649104381924763 98-99 0.025322748936678827 100-101 0.3042059108992712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2164951E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.57481402305342 #Duplication Level Percentage of deduplicated Percentage of total 1 67.99340366502466 10.589846168771581 2 11.566150943088362 3.6028129980233032 3 5.463080425482934 2.5525938485964077 4 3.0332293379430673 1.889679313109309 5 1.9945353675144308 1.5532258705719881 6 1.2033285386095454 1.1244970918485797 7 0.8911338640331808 0.9715470941373235 8 0.7964778573573134 0.9923995601456181 9 0.5841360242581578 0.8188028947787966 >10 5.0738031958720144 16.292779226942592 >50 0.7555884315117449 8.199052835664475 >100 0.4660206882996323 13.864975949520753 >500 0.08008733473429344 9.021021397612438 >1k 0.09748398623783067 26.748989467629126 >5k 0.0012768221691745602 1.222012022539636 >10k+ 2.6351786360482E-4 0.5557642601082786 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT 18508 0.15214200205163178 No Hit CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC 13819 0.11359684062845793 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13528 0.11120472248511318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1508472167294385E-4 0.0 0.0 2 1.1508472167294385E-4 0.0 0.0 3 1.1508472167294385E-4 0.0 0.0 4 1.1508472167294385E-4 0.0 0.0 5 1.2330505893529698E-4 0.0 0.0 6 1.2330505893529698E-4 0.0 0.0 7 1.2330505893529698E-4 0.0 0.0 8 1.3974573346000325E-4 0.0 0.0 9 1.8084741977176892E-4 8.220337262353132E-6 0.0 10-11 2.548304551329471E-4 8.220337262353132E-6 0.0 12-13 3.9868635722412694E-4 8.220337262353132E-6 0.0 14-15 6.740676555129568E-4 8.220337262353132E-6 0.0 16-17 0.0010686438441059073 8.220337262353132E-6 0.0 18-19 0.0015372030680600357 8.220337262353132E-6 0.0 20-21 0.0020427538096947535 8.220337262353132E-6 0.0 22-23 0.002774363826044182 8.220337262353132E-6 0.0 24-25 0.003522414516918317 8.220337262353132E-6 0.0 26-27 0.004459532964826574 8.220337262353132E-6 0.0 28-29 0.0056062700129248366 8.220337262353132E-6 0.0 30-31 0.006872201951327218 8.220337262353132E-6 0.0 32-33 0.008035379673950188 8.220337262353132E-6 0.0 34-35 0.009247879420147275 8.220337262353132E-6 0.0 36-37 0.01077275198231378 8.220337262353132E-6 0.0 38-39 0.012462031289727348 8.220337262353132E-6 0.0 40-41 0.014389700377749158 8.220337262353132E-6 0.0 42-43 0.01644478469333744 8.220337262353132E-6 0.0 44-45 0.018890335028887498 8.220337262353132E-6 0.0 46-47 0.022096266561205218 8.220337262353132E-6 0.0 48-49 0.02556935905454942 8.220337262353132E-6 0.0 50-51 0.029675417517094807 8.220337262353132E-6 0.0 52-53 0.03478846729427846 8.220337262353132E-6 0.0 54-55 0.041171559178495666 8.220337262353132E-6 0.0 56-57 0.04894388806005055 8.220337262353132E-6 0.0 58-59 0.05873841990814431 8.220337262353132E-6 0.0 60-61 0.07092506989958283 8.220337262353132E-6 0.0 62-63 0.08666701575698907 8.220337262353132E-6 0.0 64-65 0.1068150623870166 8.220337262353132E-6 0.0 66-67 0.13162404024479835 8.220337262353132E-6 0.0 68-69 0.16239276261778612 8.220337262353132E-6 0.0 70-71 0.20211754243810764 8.220337262353132E-6 0.0 72-73 0.25257808272306237 8.220337262353132E-6 0.0 74-75 0.31481836630496907 8.220337262353132E-6 0.0 76-77 0.390609875863865 8.220337262353132E-6 0.0 78-79 0.4846258731333978 8.220337262353132E-6 0.0 80-81 0.6017245774356181 8.220337262353132E-6 0.0 82-83 0.7423909886690049 8.220337262353132E-6 0.0 84-85 0.9129835377059883 8.220337262353132E-6 0.0 86-87 1.1136666312918153 8.220337262353132E-6 0.0 88-89 1.3500999716316162 8.220337262353132E-6 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTGT 43345 0.0 58.6084 1 CTTTGTG 110475 0.0 51.22512 1 TTTGTGT 122185 0.0 46.398903 2 TTGTGTT 126820 0.0 45.977356 3 TGTGTTT 134560 0.0 43.47752 4 GTGTTTG 149525 0.0 39.0885 5 TTTGAGG 54225 0.0 32.487362 8 AACTTTG 24485 0.0 32.436523 2 TTGAGGG 37110 0.0 32.3984 9 TAACTTT 24805 0.0 31.835289 1 GTTTGAG 116380 0.0 31.322273 7 TGTTTGA 194255 0.0 30.068258 6 GTTTGAC 32710 0.0 27.058609 7 TTTGACG 11650 0.0 26.166714 8 TTGACGG 7725 0.0 26.062622 9 TTGAGTC 15110 0.0 23.757902 9 TTTGACC 10000 0.0 23.551672 8 TTTGAGT 40930 0.0 23.004936 8 GTTTGAT 48790 0.0 22.675932 7 TTGACCC 6915 0.0 22.591991 9 >>END_MODULE