FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30291.34100000035bb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30291.34100000035bb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12164951
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT185080.15214200205163178No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC138190.11359684062845793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135280.11120472248511318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT433450.058.60841
CTTTGTG1104750.051.225121
TTTGTGT1221850.046.3989032
TTGTGTT1268200.045.9773563
TGTGTTT1345600.043.477524
GTGTTTG1495250.039.08855
TTTGAGG542250.032.4873628
AACTTTG244850.032.4365232
TTGAGGG371100.032.39849
TAACTTT248050.031.8352891
GTTTGAG1163800.031.3222737
TGTTTGA1942550.030.0682586
GTTTGAC327100.027.0586097
TTTGACG116500.026.1667148
TTGACGG77250.026.0626229
TTGAGTC151100.023.7579029
TTTGACC100000.023.5516728
TTTGAGT409300.023.0049368
GTTTGAT487900.022.6759327
TTGACCC69150.022.5919919
Produced by FastQC (version 0.11.2)