FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30191.34100000035b8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30191.34100000035b8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16187842
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1291300.070.981451
TTTGTGT1397400.065.7175452
TTGTGTT1588900.059.34353
TGTGTTT1718150.055.2112584
ACTTTGT339950.050.6619721
GTGTTTG1875800.050.5406845
AACTTTG162450.045.5824552
TAACTTT162500.045.4346281
TTTGAGG514350.034.5448238
GTTTGAG1422250.033.5727777
TTGAGGG266300.032.6848649
TTTGAGT544350.032.2179643
TGTTTGA2959050.032.06136
GTTTGAA283300.031.0031172
TTTGAGC386000.028.5758958
TTGAGGT143450.027.7064119
GTTTGAC677550.026.6165397
TTGACCC86900.026.2833469
GTTTGAT915150.026.0400622
TTGAGTC241550.025.9136564