##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l06n01_30112.34100000035b54.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12026478 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73475709181025 33.0 33.0 33.0 33.0 33.0 2 32.76871433182682 33.0 33.0 33.0 33.0 33.0 3 32.786050163647246 33.0 33.0 33.0 33.0 33.0 4 36.70535596539569 37.0 37.0 37.0 37.0 37.0 5 36.72848326833508 37.0 37.0 37.0 37.0 37.0 6 36.676326186269996 37.0 37.0 37.0 37.0 37.0 7 36.63990771030388 37.0 37.0 37.0 37.0 37.0 8 36.63228195320359 37.0 37.0 37.0 37.0 37.0 9 36.62339331598162 37.0 37.0 37.0 37.0 37.0 10-11 36.61387340499854 37.0 37.0 37.0 37.0 37.0 12-13 36.5679865709645 37.0 37.0 37.0 37.0 37.0 14-15 38.673126537960655 40.0 37.0 40.0 37.0 40.0 16-17 38.62716728039581 40.0 37.0 40.0 37.0 40.0 18-19 38.5873764954295 40.0 37.0 40.0 37.0 40.0 20-21 38.5407718286268 40.0 37.0 40.0 37.0 40.0 22-23 38.48169193840458 40.0 37.0 40.0 37.0 40.0 24-25 38.44277709567173 40.0 37.0 40.0 37.0 40.0 26-27 38.34493764508612 40.0 37.0 40.0 37.0 40.0 28-29 38.281315693588766 40.0 37.0 40.0 37.0 40.0 30-31 38.207308407332555 40.0 37.0 40.0 37.0 40.0 32-33 38.10795222009303 40.0 37.0 40.0 37.0 40.0 34-35 38.00568574606797 40.0 37.0 40.0 37.0 40.0 36-37 37.92056103208271 40.0 37.0 40.0 35.0 40.0 38-39 37.81880214639731 40.0 37.0 40.0 33.0 40.0 40-41 37.666638811462505 40.0 37.0 40.0 33.0 40.0 42-43 37.517670468444706 40.0 37.0 40.0 33.0 40.0 44-45 37.38102979941426 40.0 37.0 40.0 33.0 40.0 46-47 37.18282226932939 38.5 37.0 40.0 33.0 40.0 48-49 36.95572061912058 37.0 37.0 40.0 33.0 40.0 50-51 36.733235199864836 37.0 37.0 40.0 33.0 40.0 52-53 36.46883243789246 37.0 37.0 40.0 33.0 40.0 54-55 36.223434699668516 37.0 37.0 40.0 33.0 40.0 56-57 35.93515383306734 37.0 37.0 40.0 33.0 40.0 58-59 35.663290324898114 37.0 35.0 38.5 33.0 40.0 60-61 35.37746208823564 37.0 33.0 37.0 33.0 40.0 62-63 35.10734917571047 37.0 33.0 37.0 30.0 40.0 64-65 34.856690171469985 37.0 33.0 37.0 27.0 40.0 66-67 34.59345067608322 37.0 33.0 37.0 27.0 38.5 68-69 34.383435615980005 37.0 33.0 37.0 27.0 37.0 70-71 34.15749099611706 37.0 33.0 37.0 27.0 37.0 72-73 33.93608099561651 37.0 33.0 37.0 27.0 37.0 74-75 33.664652319656675 37.0 33.0 37.0 27.0 37.0 76-77 30.739679937883725 33.0 30.0 33.0 24.5 37.0 78-79 33.40895505733266 35.0 33.0 37.0 27.0 37.0 80-81 33.89067031927385 37.0 33.0 37.0 27.0 37.0 82-83 33.88032805614412 37.0 33.0 37.0 27.0 37.0 84-85 33.78033980521978 37.0 33.0 37.0 27.0 37.0 86-87 33.700736283723295 37.0 33.0 37.0 27.0 37.0 88-89 33.54025817034713 37.0 33.0 37.0 27.0 37.0 90-91 33.414215866024946 37.0 33.0 37.0 27.0 37.0 92-93 33.281373441168725 37.0 33.0 37.0 27.0 37.0 94-95 33.165195828737225 37.0 33.0 37.0 27.0 37.0 96-97 33.08172617120324 37.0 33.0 37.0 27.0 37.0 98-99 32.93295614060908 33.0 33.0 37.0 27.0 37.0 100-101 30.9675145541363 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10152143826812932 2306 2 0.0805517497259487 2306 3 0.09090164080563312 2306 4 0.051786918429961304 2306 5 0.026942862947969104 2306 6 0.06107588828065502 2306 7 0.09713595842352163 2306 8 0.0932637712564457 2306 9 0.09457780061835308 2306 10-11 0.12634179353788966 2306 12-13 0.1413343605074786 2306 14-15 0.22760217867742227 2306 16-17 0.2405318323291894 2306 18-19 0.22749447010148316 2306 20-21 0.22474565376756317 2306 22-23 0.259535013681095 2306 24-25 0.27198107232980817 2306 26-27 0.28029287786961987 2306 28-29 0.19401528165044368 2306 30-31 0.2770059106004723 2306 32-33 0.2500141015514572 2306 34-35 0.2583477861775947 2306 36-37 0.2763221110740659 2306 38-39 0.28483518959048126 2306 40-41 0.1985141736292988 2306 42-43 0.3032560806980271 2306 44-45 0.27126164961467936 2306 46-47 0.32247965144363633 2306 48-49 0.23917849928064783 2306 50-51 0.32856560323653383 2306 52-53 0.30156292202831736 2306 54-55 0.2727148802670101 2306 56-57 0.20194831381592593 2306 58-59 0.2061854070536313 2306 60-61 0.1761291694291387 2306 62-63 0.17648304763601175 2306 64-65 0.2191804288143686 2306 66-67 0.1320962666330061 2306 68-69 0.08357933798993855 2306 70-71 0.13018516818071646 2306 72-73 0.07732302840746996 2306 74-75 0.07540370494195514 2306 76-77 -0.23126405269237438 2306 78-79 0.1788730563768084 2306 80-81 0.29389086274118625 2306 82-83 0.2714638485866274 2306 84-85 0.30744397602159523 2306 86-87 0.24544077435882627 2306 88-89 0.26998496992426624 2306 90-91 0.0805573537526314 2306 92-93 0.23548244203104218 2306 94-95 0.19368490461676657 2306 96-97 0.14802217046008082 2306 98-99 0.19426342362216076 2306 100-101 0.23855508720933116 2307 1 0.12037391323420366 2307 2 0.10144469973029402 2307 3 0.06691941074732455 2307 4 0.04971536601635762 2307 5 0.06963038755324646 2307 6 0.08421496827754282 2307 7 0.08755844434281812 2307 8 0.12615655036342588 2307 9 0.11128201676347516 2307 10-11 0.07545738558163606 2307 12-13 0.10116383539571672 2307 14-15 0.1539788894841223 2307 16-17 0.2027678720450652 2307 18-19 0.1363142828024806 2307 20-21 0.15305898985329947 2307 22-23 0.18609114556873152 2307 24-25 0.1762809035757158 2307 26-27 0.20182550149947787 2307 28-29 0.16762426594480928 2307 30-31 0.11698135608985893 2307 32-33 0.15625324421070985 2307 34-35 0.05908498551385577 2307 36-37 0.06815227942206548 2307 38-39 0.03214481810586278 2307 40-41 0.07194795992525371 2307 42-43 0.04513706704090481 2307 44-45 0.06928367478791841 2307 46-47 0.08413805948111275 2307 48-49 0.008462447788090799 2307 50-51 -0.0766656045396914 2307 52-53 0.01940737045848806 2307 54-55 0.02901899776542649 2307 56-57 -0.023892121910300546 2307 58-59 -0.0633917601561862 2307 60-61 -0.09221719130304962 2307 62-63 -0.10034407360636521 2307 64-65 -0.11320250649166752 2307 66-67 -0.09198741610929773 2307 68-69 -0.2893586696964974 2307 70-71 -0.2209159776776417 2307 72-73 -0.3600252719815913 2307 74-75 -0.26245446039870757 2307 76-77 -0.32007986368778774 2307 78-79 -0.13461323432694172 2307 80-81 -0.008015072924699496 2307 82-83 -0.11057512757460586 2307 84-85 -0.16079446697369804 2307 86-87 -0.12183570119608333 2307 88-89 -0.10753273459137347 2307 90-91 -0.16399779561617578 2307 92-93 -0.020811621851862583 2307 94-95 -0.20684172576100224 2307 96-97 -0.29785213170901415 2307 98-99 -0.1792770466219551 2307 100-101 -0.18319529660471545 2201 1 0.10570443222547254 2201 2 0.08382237557127326 2201 3 0.07969610513882941 2201 4 0.053898591257294015 2201 5 0.05993118728929403 2201 6 0.10042156511504174 2201 7 0.07275846453319446 2201 8 0.07916590195674189 2201 9 0.06595898298644443 2201 10-11 0.060427493969505974 2201 12-13 0.07494564140063176 2201 14-15 0.15368772942364473 2201 16-17 0.15417953666806739 2201 18-19 0.1790208156217048 2201 20-21 0.15994015469051703 2201 22-23 0.06853958007557281 2201 24-25 0.10504154661471432 2201 26-27 0.13323794788416876 2201 28-29 0.12360883630827857 2201 30-31 0.07797237137052804 2201 32-33 0.13435409885474314 2201 34-35 0.09768320679058462 2201 36-37 0.1038205256166691 2201 38-39 0.09588791151838194 2201 40-41 0.09829077411958309 2201 42-43 0.14056342262797727 2201 44-45 0.10491209422583125 2201 46-47 0.09408142177040446 2201 48-49 0.03181908623746921 2201 50-51 0.04788959152860173 2201 52-53 -6.640837906033425E-4 2201 54-55 0.03205127898296212 2201 56-57 0.08949191513165289 2201 58-59 -0.0611336384525103 2201 60-61 -0.06611949325264987 2201 62-63 -0.04738353595129752 2201 64-65 -0.07502680586084409 2201 66-67 -0.014598487824542872 2201 68-69 -0.18892987033471087 2201 70-71 -0.38834378475609554 2201 72-73 -0.3262240204242275 2201 74-75 -0.31581443518535934 2201 76-77 -0.8102670925334365 2201 78-79 -0.1930480427891652 2201 80-81 0.05450748736328137 2201 82-83 0.05975076855820305 2201 84-85 -0.08014411511938135 2201 86-87 -0.08750993063760149 2201 88-89 -0.10215501443004626 2201 90-91 -0.1112912073965262 2201 92-93 -0.07477407636194755 2201 94-95 -0.2587712921110281 2201 96-97 -0.33790027468646144 2201 98-99 -0.2817448589696099 2201 100-101 -0.3063371660369718 1101 1 0.08270435288284972 1101 2 0.077810599928263 1101 3 0.05995928645202753 1101 4 0.05002404309024655 1101 5 0.053113676488628414 1101 6 0.0571660291373135 1101 7 0.05069316438936511 1101 8 0.034847507492166585 1101 9 0.08127593514517173 1101 10-11 0.06766132899107191 1101 12-13 0.10250036992992761 1101 14-15 0.11726365989888166 1101 16-17 0.08038409326339746 1101 18-19 0.08247820788415083 1101 20-21 0.08073673800085857 1101 22-23 0.08232380573065257 1101 24-25 -0.03598793806908418 1101 26-27 0.054347294162489845 1101 28-29 0.07575826458396762 1101 30-31 0.02742035795579767 1101 32-33 0.04202717929968003 1101 34-35 0.03966633325922686 1101 36-37 -0.09573896480074495 1101 38-39 -0.1894400404888117 1101 40-41 -0.09737567148555826 1101 42-43 -0.1462367425776634 1101 44-45 -0.18979289176778025 1101 46-47 -0.09634486768445782 1101 48-49 -0.06960948020100233 1101 50-51 -0.07836405103803656 1101 52-53 -0.11465561492193643 1101 54-55 -0.11206839298103688 1101 56-57 -0.09145101789402332 1101 58-59 -0.1215948137113756 1101 60-61 -0.08715405453368419 1101 62-63 -0.12467456253340714 1101 64-65 -0.21634450221013424 1101 66-67 -0.20436874518573234 1101 68-69 -0.3586410482133928 1101 70-71 -0.3882619637148039 1101 72-73 -0.37160462466238187 1101 74-75 -0.3847177321624713 1101 76-77 -0.7285616719019288 1101 78-79 -0.27035625195628654 1101 80-81 -0.046292658879977466 1101 82-83 -0.08601573691153419 1101 84-85 -0.11758387003218473 1101 86-87 -0.1649192226402434 1101 88-89 -0.08982277542611428 1101 90-91 -0.19274389598869845 1101 92-93 -0.17671025262299622 1101 94-95 -0.26384685805930275 1101 96-97 -0.27875409114152205 1101 98-99 -0.27226446333434495 1101 100-101 -0.40649076138060636 2304 1 0.08740606896929393 2304 2 0.055165659153360025 2304 3 0.08170762563618439 2304 4 0.024609929894218396 2304 5 0.026058681861520938 2304 6 0.040431819799749746 2304 7 0.034209021731101075 2304 8 -0.007131596060418133 2304 9 0.059634406192479616 2304 10-11 0.031570170281980836 2304 12-13 0.02002275182275781 2304 14-15 0.0768377602678072 2304 16-17 0.06330452364942118 2304 18-19 0.09825652115201677 2304 20-21 0.09498890450024788 2304 22-23 0.08780476782443003 2304 24-25 0.030327756467222855 2304 26-27 0.08378080525591969 2304 28-29 0.017310296799706748 2304 30-31 0.017947055117652155 2304 32-33 0.0380376136479299 2304 34-35 0.07367739046159727 2304 36-37 -0.05936900178656401 2304 38-39 -0.08026874481348045 2304 40-41 -0.004387621342722525 2304 42-43 -0.042284255246734403 2304 44-45 -0.07649688343017402 2304 46-47 -0.05238431716774983 2304 48-49 -0.025189590187174815 2304 50-51 -0.05976889542471753 2304 52-53 -0.04490455614701716 2304 54-55 -0.046636041259624506 2304 56-57 -0.035890672734169016 2304 58-59 -0.10352458228407357 2304 60-61 -0.15415572113096943 2304 62-63 -0.16148869453839154 2304 64-65 -0.08458937946624445 2304 66-67 -0.15844127068960745 2304 68-69 -0.2116250898891181 2304 70-71 -0.2670758891295577 2304 72-73 -0.32244476853551873 2304 74-75 -0.45224860892215446 2304 76-77 -0.8211397021103508 2304 78-79 -0.31926742895014826 2304 80-81 -0.05127166838774855 2304 82-83 -0.06499562828207672 2304 84-85 -0.1053229712413497 2304 86-87 -0.20493904913528382 2304 88-89 -0.15584381679502712 2304 90-91 -0.15815904213587118 2304 92-93 -0.14505856374788095 2304 94-95 -0.22956931487963317 2304 96-97 -0.24710785994855655 2304 98-99 -0.28446891982475364 2304 100-101 -0.32952557183407194 2202 1 0.08033323609689802 2202 2 0.07765214915859531 2202 3 0.08176491088590154 2202 4 0.005432275736680481 2202 5 0.032830288519399176 2202 6 0.03382770357119114 2202 7 0.0341833276117427 2202 8 -0.0033070780884116857 2202 9 0.01673181313505978 2202 10-11 0.04366306352215332 2202 12-13 0.06064637030912223 2202 14-15 0.060710851996006454 2202 16-17 0.06709854182102504 2202 18-19 0.018106817486398086 2202 20-21 0.02868918317231106 2202 22-23 -0.006207659934794663 2202 24-25 -0.006587101607237855 2202 26-27 0.06937231087962203 2202 28-29 3.281304714448652E-4 2202 30-31 0.006049921581947615 2202 32-33 0.051605659506762436 2202 34-35 0.061583118699374495 2202 36-37 -0.07177516416305707 2202 38-39 -0.010538935135684824 2202 40-41 0.06092425491195286 2202 42-43 0.00897087950858122 2202 44-45 0.044579617842565256 2202 46-47 0.07998156173099602 2202 48-49 -0.010012770801317572 2202 50-51 -0.03483130705331661 2202 52-53 -0.07244352093439232 2202 54-55 -0.04979452247295768 2202 56-57 0.026955965730230957 2202 58-59 -0.09062845104877937 2202 60-61 -0.10605775607841395 2202 62-63 -0.10709997812431027 2202 64-65 -0.10062066234376488 2202 66-67 -0.045257717606453696 2202 68-69 -0.1643843549453976 2202 70-71 -0.13630336154236744 2202 72-73 -0.13416259847376466 2202 74-75 -0.09914703003574488 2202 76-77 -0.12026442676493332 2202 78-79 0.03621292376003282 2202 80-81 0.13905170033721959 2202 82-83 0.061548091449076026 2202 84-85 0.09636964323102148 2202 86-87 0.1073242830397092 2202 88-89 0.006245084475025919 2202 90-91 0.050031657243891914 2202 92-93 0.08514668286875349 2202 94-95 0.009555448067224859 2202 96-97 -0.1384225009057829 2202 98-99 -0.06795787298084832 2202 100-101 -0.025205771949526223 1102 1 0.06217778212144509 1102 2 0.046468910893189275 1102 3 0.03160857201824996 1102 4 -0.029654890360980346 1102 5 0.024495699498430668 1102 6 0.033494292375188195 1102 7 -0.005141106654768635 1102 8 -0.0166702222078996 1102 9 0.008993063101264909 1102 10-11 0.004557699950886729 1102 12-13 0.05634695844569393 1102 14-15 0.036637353720180954 1102 16-17 0.010293075641804705 1102 18-19 0.021355791876288777 1102 20-21 -6.739339497414676E-4 1102 22-23 0.041258315772410015 1102 24-25 0.003316385408680844 1102 26-27 -0.060807072882383295 1102 28-29 -0.049510093973367475 1102 30-31 -0.03893167038574319 1102 32-33 -0.09784967793686405 1102 34-35 -0.04300921884136244 1102 36-37 -0.14584155524098463 1102 38-39 -0.1288880584826657 1102 40-41 -0.14734131173809573 1102 42-43 -0.16066965287652835 1102 44-45 -0.102732213866922 1102 46-47 -0.13541260236669217 1102 48-49 -0.1626992614955114 1102 50-51 -0.1957749817304304 1102 52-53 -0.08615110374415025 1102 54-55 -0.06528366119713525 1102 56-57 -0.13286650064671335 1102 58-59 -0.14342086591837955 1102 60-61 -0.2775458410524436 1102 62-63 -0.2914342347438321 1102 64-65 -0.2323717253322073 1102 66-67 -0.2323887859214011 1102 68-69 -0.24339297665671467 1102 70-71 -0.31023876193181366 1102 72-73 -0.33149542759522177 1102 74-75 -0.39158108482288867 1102 76-77 -0.5636849577874088 1102 78-79 -0.32718853049453855 1102 80-81 -0.1959793947165238 1102 82-83 -0.2603165563246108 1102 84-85 -0.28319099624247457 1102 86-87 -0.30272500427947335 1102 88-89 -0.3143881509152635 1102 90-91 -0.4436918916060648 1102 92-93 -0.3005093708429598 1102 94-95 -0.50831531055033 1102 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44-45 -0.26873736737849896 1213 46-47 -0.3277363553717052 1213 48-49 -0.2763446812323238 1213 50-51 -0.22124036359087995 1213 52-53 -0.1733244672195653 1213 54-55 -0.6719911926256685 1213 56-57 -1.6221092760628224 1213 58-59 -0.5506523908068033 1213 60-61 -0.371741299367109 1213 62-63 -0.229655495281456 1213 64-65 -1.6058496004306164 1213 66-67 -0.6480946737750344 1213 68-69 -0.2808368124281415 1213 70-71 -0.1457314293441172 1213 72-73 -0.9891665656879169 1213 74-75 -0.4219965507011594 1213 76-77 -0.40842139364185215 1213 78-79 -0.46904490530334186 1213 80-81 -0.3369909086467189 1213 82-83 -1.318225239902958 1213 84-85 -0.5906612996621519 1213 86-87 -0.39800736982443397 1213 88-89 -1.209705465065987 1213 90-91 -1.4431380943857377 1213 92-93 -2.5034401564890914 1213 94-95 -1.1400397746402575 1213 96-97 -0.43041135656373086 1213 98-99 -0.13963244176606793 1213 100-101 0.014921885057805895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 19.0 8 257.0 9 770.0 10 490.0 11 159.0 12 212.0 13 420.0 14 1291.0 15 2979.0 16 5733.0 17 9141.0 18 12543.0 19 16365.0 20 20346.0 21 24955.0 22 31160.0 23 38008.0 24 46651.0 25 57992.0 26 71806.0 27 89520.0 28 112563.0 29 142118.0 30 181028.0 31 236347.0 32 318261.0 33 452404.0 34 719643.0 35 1388840.0 36 3132421.0 37 4208903.0 38 702992.0 39 140.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.537257787654276 22.00217824137073 31.123135095269326 27.337428875705662 2 26.559562990927187 21.89765781802453 32.29983873915538 19.2429404518929 3 20.83235840118778 20.270032506607507 39.61613699372335 19.281472098481366 4 20.681636458169734 19.961095287830698 38.8323442777389 20.524923976260666 5 29.546355965561986 19.358601911548835 32.40632876890474 18.688713353984436 6 29.17485069194822 19.738272501724943 32.85231968993749 18.234557116389354 7 29.943912091303872 25.47788305104786 25.9607592513785 18.61744560626977 8 27.01634676419813 21.1813716368167 32.189656855481715 19.61262474350346 9 22.23011591589824 20.361222961535372 36.7146640936773 20.69399702888909 10-11 28.231219859640937 21.137903557669304 29.49206668872362 21.138809893966137 12-13 26.842390883400963 26.232726783200555 25.006941985456482 21.917940347941997 14-15 26.853955684328533 23.145788626748846 26.200481318806045 23.799774370116573 16-17 26.457958015638493 22.8933441694235 26.137448553100917 24.51124926183709 18-19 25.996592684907416 23.739032325174502 26.73996077654655 23.52441421337153 20-21 24.478309460103482 24.827728049937445 27.298466087937406 23.395496402021667 22-23 24.536302315607276 24.678896015940825 27.034469276873914 23.750332391577984 24-25 24.746621579484867 24.976273186547218 26.586428711714273 23.690676522253646 26-27 24.57811422429742 24.802739422131733 26.540268065180843 24.07887828839 28-29 24.764643008757893 24.968277252221547 26.199865305385465 24.06721443363509 30-31 24.903051352558148 24.923897037118955 25.92149234147676 24.251559268846137 32-33 24.844634480685034 24.94681734752269 25.816826838248076 24.3917213335442 34-35 24.72199674750995 25.18652177304112 25.953134408926704 24.138347070522226 36-37 24.866948840587145 25.187984523035134 25.8841120140052 24.060954622372517 38-39 25.027608574810483 24.939039119319517 25.798798535358973 24.234553770511024 40-41 24.610871828456197 25.207691001904607 25.891120070242486 24.290317099396702 42-43 24.672380897264418 25.333427126472564 25.713326757835592 24.280865218427426 44-45 24.696839724455348 24.91140454709128 26.165096881781807 24.22665884667157 46-47 24.39011957391308 25.1547607974987 25.96638522342529 24.48873440516293 48-49 24.748062656878766 24.869728154135295 25.878309057886717 24.50390013109922 50-51 24.628084219571726 25.31737712712701 26.1985230263296 23.85601562697166 52-53 24.97095206345224 25.12100309976313 25.972308139662754 23.93573669712187 54-55 24.486724398711843 25.072584995439296 25.835888516432128 24.60480208941673 56-57 24.80007457844943 25.086334243429363 25.967196542613603 24.1463946355076 58-59 24.50142049713262 24.951111869955227 26.07059775731902 24.476869875593135 60-61 24.733721098918725 25.02067633603891 25.789414838588616 24.45618772645375 62-63 24.24906242726374 25.331723084018275 25.9409676148128 24.47824687390519 64-65 24.621224862649044 25.169173428621267 25.754763443679092 24.4548382650506 66-67 24.60369004929891 25.23598620602013 25.72492913219752 24.435394612483446 68-69 24.519689417661024 25.35698065517761 25.91795114722738 24.205378779933987 70-71 24.40085159364869 25.54528970683038 25.668271159446032 24.385587540074898 72-73 24.725787144126226 25.217499092263758 25.573129610098384 24.48358415351163 74-75 24.77836307288191 25.10850848939416 25.621239586611516 24.491888851112414 76-77 24.711458022062754 24.979755325469196 25.709840402801543 24.598946249666508 78-79 25.033084520884458 24.872899354831574 25.671692376297816 24.422323747986148 80-81 24.848832744324284 25.03214175064986 25.58731139639796 24.53171410862789 82-83 24.657424270884547 25.5175253541836 25.62311876144313 24.201931613488718 84-85 24.42490731675028 25.662932005562244 25.65853712893229 24.25362354875519 86-87 24.424200802015157 25.66336237441329 25.640908145487767 24.271528678083783 88-89 24.43834029918619 26.283810680440272 25.167487556294045 24.11036146407949 90-91 24.106032835152206 25.86429935162449 25.498596616168523 24.531071197054786 92-93 24.13796759955788 26.32031094710456 25.11946613040184 24.42225532293572 94-95 24.201198674002857 26.148860945298622 25.12034897918581 24.529591401512707 96-97 24.035558293968077 26.03872941669831 25.06901560580467 24.85669668352895 98-99 24.30683077740962 25.89074468871686 25.05993051777655 24.742494016096966 100-101 24.752348782853865 26.461894983482136 24.43494789223482 24.350808341429182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 20060.0 1 11886.0 2 6059.5 3 7378.0 4 7629.0 5 6298.0 6 3444.0 7 3217.5 8 3365.0 9 3501.0 10 3532.5 11 3397.0 12 3156.5 13 3003.0 14 3014.5 15 3119.0 16 3318.5 17 3625.0 18 3956.0 19 4338.5 20 4848.5 21 5369.5 22 6016.0 23 7044.0 24 8193.0 25 9517.5 26 10986.5 27 12696.5 28 15160.0 29 18255.5 30 21757.5 31 26382.5 32 35007.5 33 48148.0 34 63442.5 35 88805.0 36 118750.0 37 160654.5 38 219451.0 39 254280.0 40 271395.5 41 280851.0 42 295811.5 43 339295.5 44 400239.0 45 465086.5 46 525981.5 47 567246.5 48 604281.5 49 651284.5 50 695496.0 51 733544.0 52 729418.0 53 685282.0 54 641233.5 55 595152.5 56 532720.0 57 444087.5 58 343160.5 59 265697.5 60 198288.0 61 141904.0 62 106234.5 63 75350.5 64 50001.0 65 34282.0 66 24933.0 67 18425.0 68 14884.0 69 11741.5 70 8798.0 71 7233.5 72 6597.5 73 5655.5 74 4223.5 75 3203.0 76 2638.5 77 2232.0 78 2028.0 79 1873.5 80 1488.0 81 1078.0 82 785.5 83 612.5 84 459.5 85 323.0 86 268.5 87 253.5 88 255.5 89 244.5 90 201.0 91 154.0 92 122.5 93 95.0 94 79.5 95 78.0 96 71.0 97 46.0 98 22.0 99 8.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15487493512231928 2 0.0 3 0.0 4 0.09546435789430621 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.076544936929997E-4 12-13 1.6214223316252688E-4 14-15 0.0012098305089819313 16-17 0.0 18-19 0.0 20-21 5.945215215959319E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1225231526636477E-4 30-31 6.651989052821616E-5 32-33 0.0 34-35 0.0 36-37 7.400337821264047E-4 38-39 0.0026857405800767274 40-41 0.0011516256047697422 42-43 9.977983579232423E-5 44-45 3.533869184311483E-4 46-47 0.007512590136530413 48-49 0.003924673541164753 50-51 0.005018094241722307 52-53 0.0019664942637403903 54-55 0.007658102397060886 56-57 0.012942276200896056 58-59 0.019477855445293294 60-61 0.010456095292403976 62-63 0.008406451165503318 64-65 0.009732691482909625 66-67 0.022300793299584468 68-69 0.018517474525792173 70-71 0.012422589556144368 72-73 0.01712887181101566 74-75 0.021070175324812465 76-77 0.034760800294150955 78-79 6.943013573882561E-4 80-81 5.321591242257292E-4 82-83 0.0013220828242482962 84-85 0.009246264783422045 86-87 0.016771327399426498 88-89 0.01851331703263416 90-91 0.02082488322848967 92-93 0.021352884859557386 94-95 0.02931864175031127 96-97 0.027368777459202936 98-99 0.02470382434491628 100-101 0.30165523106598624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2026478E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.131985080375436 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7202037744442 20.54439439091208 2 11.671502277000048 6.333420512902664 3 4.084003104353813 3.3242133388660373 4 1.9964710228300944 2.1667288801931206 5 1.307250384215842 1.773414896042964 6 0.7265110264690302 1.1827011798531584 7 0.5496594215944931 1.0439345858192706 8 0.550620459485395 1.1951540873365762 9 0.31373459761979083 0.766101817963598 >10 2.1442994173126415 11.604849819998488 >50 0.3883730684286964 7.5331730660413045 >100 0.4851346401934733 27.358475595299307 >500 0.04938009270322088 9.274383677205012 >1k 0.012548764599614438 5.096808340035794 >5k 2.4665355868746944E-4 0.5228440850902744 >10k+ 6.129519102928984E-5 0.2794017264406022 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT 19316 0.16061227568037792 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14285 0.11877957952444598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1640980842437828E-4 0.0 0.0 2 1.247247947404053E-4 0.0 0.0 3 1.330397810564323E-4 0.0 0.0 4 1.330397810564323E-4 0.0 0.0 5 1.330397810564323E-4 0.0 0.0 6 1.4135476737245933E-4 0.0 0.0 7 1.4966975368848636E-4 0.0 0.0 8 1.8292969895259442E-4 0.0 0.0 9 2.4113460316478358E-4 0.0 0.0 10-11 2.8270953474491867E-4 0.0 0.0 12-13 4.282217952753915E-4 0.0 0.0 14-15 7.109313300203103E-4 0.0 8.31498631602702E-6 16-17 9.811683852911884E-4 0.0 8.31498631602702E-6 18-19 0.0013511852763543907 0.0 1.2472479474040529E-5 20-21 0.0016920997153114985 0.0 2.4944958948081058E-5 22-23 0.0021078490311128495 0.0 2.4944958948081058E-5 24-25 0.0026150631963904976 0.0 2.4944958948081058E-5 26-27 0.0030723874437719838 0.0 2.4944958948081058E-5 28-29 0.0037583738148442127 0.0 2.4944958948081058E-5 30-31 0.004456832665390482 0.0 2.4944958948081058E-5 32-33 0.005242598872255037 0.0 2.4944958948081058E-5 34-35 0.006036680065435616 0.0 2.9102452106094566E-5 36-37 0.0070885258344130335 0.0 3.741743842212159E-5 38-39 0.008152844082864492 0.0 4.15749315801351E-5 40-41 0.009624596660801276 0.0 4.15749315801351E-5 42-43 0.011246018992426544 0.0 4.15749315801351E-5 44-45 0.013345553037223366 0.0 4.15749315801351E-5 46-47 0.01573195410992312 0.0 4.15749315801351E-5 48-49 0.018675459265796685 0.0 4.15749315801351E-5 50-51 0.021839311559044967 0.0 4.15749315801351E-5 52-53 0.026050852128112652 0.0 4.15749315801351E-5 54-55 0.031517955630900416 0.0 4.9889917896162117E-5 56-57 0.03821567710846018 0.0 4.9889917896162117E-5 58-59 0.046260426369216325 0.0 5.820490421218914E-5 60-61 0.05586423556422753 0.0 6.236239737020264E-5 62-63 0.06755510632456152 0.0 6.651989052821616E-5 64-65 0.08209801739129277 0.0 7.067738368622967E-5 66-67 0.09955533116179151 0.0 7.483487684424318E-5 68-69 0.12218872391401706 0.0 7.899237000225669E-5 70-71 0.14896713734478206 0.0 9.562234263431072E-5 72-73 0.1816866084983484 0.0 9.977983579232423E-5 74-75 0.2208959264715738 0.0 1.0809482210835126E-4 76-77 0.26915610704979465 0.0 1.0809482210835126E-4 78-79 0.32778923305725916 0.0 1.1640980842437828E-4 80-81 0.39844998677085675 0.0 1.1640980842437828E-4 82-83 0.4835330842496033 0.0 1.1640980842437828E-4 84-85 0.5863478900472774 0.0 1.1640980842437828E-4 86-87 0.7103409659918722 0.0 1.1640980842437828E-4 88-89 0.8580525404029342 0.0 1.1640980842437828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 151850 0.0 63.179543 1 TTTGTGT 165480 0.0 58.088425 2 TTGTGTT 178750 0.0 55.203964 3 TGTGTTT 192090 0.0 51.659737 4 ACTTTGT 44880 0.0 49.373833 1 GTGTTTG 206175 0.0 48.1283 5 TAACTTT 24515 0.0 47.825832 1 AACTTTG 25145 0.0 47.27484 2 TTGAGGG 33110 0.0 36.973053 9 TTTGAGG 60625 0.0 36.30367 8 GTTTGAG 161555 0.0 35.156013 7 TGTTTGA 282475 0.0 35.089584 6 GTTTGAC 56240 0.0 32.844063 7 GTTTGAT 67810 0.0 29.368868 7 TTGAGGC 12690 0.0 29.224764 9 TTTGACC 20035 0.0 29.199398 8 TTTGATG 19490 0.0 27.433367 8 TTGACCC 12410 0.0 27.39699 9 TTGAGTC 22045 0.0 27.058372 4 TTTGAGA 22345 0.0 26.83957 8 >>END_MODULE