FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l06n01_30112.34100000035b54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l06n01_30112.34100000035b54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12026478
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT193160.16061227568037792No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142850.11877957952444598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1518500.063.1795431
TTTGTGT1654800.058.0884252
TTGTGTT1787500.055.2039643
TGTGTTT1920900.051.6597374
ACTTTGT448800.049.3738331
GTGTTTG2061750.048.12835
TAACTTT245150.047.8258321
AACTTTG251450.047.274842
TTGAGGG331100.036.9730539
TTTGAGG606250.036.303678
GTTTGAG1615550.035.1560137
TGTTTGA2824750.035.0895846
GTTTGAC562400.032.8440637
GTTTGAT678100.029.3688687
TTGAGGC126900.029.2247649
TTTGACC200350.029.1993988
TTTGATG194900.027.4333678
TTGACCC124100.027.396999
TTGAGTC220450.027.0583724
TTTGAGA223450.026.839578
Produced by FastQC (version 0.11.2)