FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30427.34200000035d1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30427.34200000035d1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22972646
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG7958.8484245E-5157.4622794-95
AGATCGA12403.0806714E-6134.6048494-95
CGTCGTA18601.7131242E-7112.170794-95
TGATACA24008.176215E-569.54584594-95
TAACTTT241100.062.1473921
AACTTTG251000.059.9777762
AGCACGA21350.004485939658.63349594-95
TTTTCGA28651.9637951E-458.25829394-95
TGCAGGG22000.005051411756.90114294-95
AGTTAGG23650.00672475452.93129394-95
CCCTCCG24000.007126988452.1593894-95
GGCTTTA34404.8449804E-448.5203594-95
TTCCTCA34554.9500586E-448.309794-95
GGAATAA26050.00985348548.05470794-95
ACTTTGT506650.046.4487341
CGATCTA9350.044.6283594-95
CTTTGTG1008250.043.4629331
TTTGTGT1085000.040.433882
TCCGATC18150.038.4249427
TGTGGAA43900.001609197538.02050494-95
Produced by FastQC (version 0.11.2)