FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30348.34200000035cf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30348.34200000035cf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16874426
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG175820.1041931737411394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATG15408.827854E-6108.9056794-95
GTCTAAA12004.4695463E-4104.821794-95
AATGGTC17350.001933361372.4991794-95
CTTTGTG1889450.072.485541
TTTGTGT2017550.067.928332
ACATCCA40302.2525455E-762.4248494-95
TCGGATG20300.003602973761.9635794-95
TTGTGTT2277700.061.4659083
TAACTTT170100.061.0313841
GACTCCG14150.059.26315794-95
GTGATTA21250.004318447359.19343694-95
AACTTTG179550.058.288392
TGTGTTT2421350.058.1373634
GTGTTTG2655400.053.021825
ACTTTGT406800.052.0337941
CACTGCA25000.00821397950.31441594-95
GAATCGG8801.2127504E-547.6462394-95
TGCCGAA35855.7955517E-446.78235294-95
AGTTCTG35905.8355025E-446.71719494-95
GATTCGA9103.6929423E-446.07547494-95
Produced by FastQC (version 0.11.2)