FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30304.34200000035cbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30304.34200000035cbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19625347
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTAG5600.006446758149.6660294-95
GGCCTCA10452.5840304E-4120.30569594-95
GGATGTA17550.002027992971.63502594-95
CTTTGTG2076450.062.961051
TTTGTGT2192800.059.6360932
TAACTTT222350.059.0229571
ACTTTGT643300.058.83171
AACTTTG230300.057.3921242
TCGCTGA22950.00586979554.7797294-95
TTGTGTT2471750.054.292523
GTGTCAA23350.00628505453.84131294-95
GTACACA23600.00655545453.27095894-95
TGTGTTT2609150.051.7392164
TACAATA24650.007786717751.0018194-95
TGGTCTA24900.008103589550.48974294-95
GATGACA25200.00849638549.88867694-95
GGTTCTA33604.2225386E-449.88867694-95
GTGTTTG3158300.042.8166545
CGACTCG19950.042.01151394-95
TTTGAGG893850.035.286848
Produced by FastQC (version 0.11.2)