FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30301.34200000035c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30301.34200000035c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14269267
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216600.15179476282839197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCG15450.001191187481.90217694-95
GGTACGA16850.001680798175.0972594-95
CTTTGTG1144550.062.9398881
TTTGTGT1207700.059.710932
ACTTTGT351850.057.5439641
TTGTGTT1378500.053.70393
TGTGTTT1465850.050.776244
GTGTTTG1617600.046.0128335
AACTTTG147200.044.9151572
TAACTTT153950.042.8218771
CTAGTTA31800.039.792194-95
TTTGAGG472900.032.750218
GTTTGAG1251950.032.5102777
TTGAGGG226200.031.7162659
GAGCCGG13300.031.71400594-95
TGTTTGA2393900.031.105276
GACAACG69403.9040178E-430.38877994-95
AGTTCTG55750.0049190730.26340794-95
CAACCTA56000.005027883730.12830294-95
TTTGAGT477150.029.6446633
Produced by FastQC (version 0.11.2)