FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30295.34200000035c36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30295.34200000035c36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13629433
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA616260.45215380566454966No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408110.2994328524157975No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC239130.17545117247357245No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC165600.12150175286088571No Hit
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT153000.1122570542736444No Hit
CCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA143320.10515477789868441No Hit
TTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACT140370.10299034449929062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC5802.569497E-5214.6771994-95
AGGATCG4600.003683254180.453394-95
GCCCCAT5400.00594994153.7194794-95
GAGATCA8109.730933E-5153.7194794-95
TCCCTCG6000.008152955138.3475294-95
TAAATCG6050.008357728137.2041594-95
CCTCGCA6150.008777465134.9731894-95
CTATATG6400.00988753129.700894-95
TATATTG13655.0949056E-6121.6241894-95
CAGATAG11654.135733E-4106.87791494-95
GAGCGCG4200.00787502598.8196594-95
TAGATAA13357.1081094E-493.2679894-95
GAAATTA14209.0839056E-487.6850494-95
TTTAGCG14259.211638E-487.3773894-95
AAGAGCC14309.3407097E-487.0718694-95
TATTATC15750.00137071579.05572594-95
ATATTAA21304.6421355E-577.9422694-95
TAGCGAC5850.00736712670.9474494-95
ATGCTTC17850.002252291469.7550594-95
TTTCTAA18050.002354038468.9821494-95
Produced by FastQC (version 0.11.2)