FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30292.34200000035c0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30292.34200000035c0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535871
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA992420.6387926367308276No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT619940.3990378138438456No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC234500.15094100613991968No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC174750.11248162397846893No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT169960.10939843668887313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG2657.007906E-4314.1287294-95
ACTAACG6451.2010605E-7258.1212894-95
AATCCCG5051.4625772E-5247.2597494-95
AGATCGT4150.0026842477200.5882394-95
ATAACTC4250.002882484195.868594-95
ATATTAG6403.7631205E-5195.1033894-95
CCGCGGA4400.0031977235189.1911894-95
ATATTAA13251.03682396E-10188.4772394-95
CGCCTAA4800.004148528173.4252594-95
ACCATTA5000.004687314166.4882294-95
CGATAGG5150.0051205726161.6390594-95
GCGTCGA8201.01061E-4152.2758294-95
CGAGCAA5600.0065782326148.650294-95
GCGCAAA8601.221768E-4145.1932294-95
CCTGGCA8651.2503099E-4144.3539494-95
AGAACCG10202.4102525E-4122.41781694-95
GCTATAA10352.554455E-4120.6436494-95
CAACGGG14055.801705E-6118.4969594-95
AATTTAG11353.6871206E-4110.01424494-95
GAAGATA15459.302545E-6107.7593794-95
Produced by FastQC (version 0.11.2)