FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30291.34200000035bbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30291.34200000035bbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12310450
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226900.18431495193108294No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT216700.1760293084330792No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC156370.12702216409635717No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138940.11286346153065079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG4550.0035310634183.0099394-95
GAGCGTA9809.638425E-7169.937894-95
GGTAGCA12856.030397E-497.20177594-95
AGGGTTA19502.948758E-585.4046494-95
TCGGAAA17150.001897627672.8304994-95
TCAATTA30653.3824726E-667.9196894-95
TAAAGTG19250.003000060864.88534594-95
GGACTAG19800.003354365763.08297394-95
CGGCCTA20550.00388662860.78067494-95
ACTTTGT450700.058.6411
CTTTGTG1178650.051.2999951
AGTTCTG24550.00785605650.8775194-95
ACTAACG8705.565702E-547.8560594-95
TTTGTGT1311750.046.1402442
TTGTGTT1347850.045.9335983
AACACCG37507.482205E-444.4104194-95
TGTGTTT1434100.043.3701974
GTTTGGA70152.2503264E-741.54573494-95
CTACCGG20400.040.81839494-95
GTGTTTG1625350.038.2497945
Produced by FastQC (version 0.11.2)