FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30191.34200000035b87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30191.34200000035b87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16297633
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGC16350.001513696277.10979594-95
ACATCCG16800.001685907475.0443594-95
CTTTGTG1350850.069.6633151
TTTGTGT1464000.064.334712
GTTCTCA49201.2827513E-859.7914394-95
TTGTGTT1651950.058.3782463
AGCGCCA22600.005460696355.7851894-95
TGTGTTT1781300.054.4521984
ACTTTGT349800.050.3573271
GAGCCGG16700.050.3291494-95
CTAGTTA25400.00866644949.6356394-95
GTGTTTG1955450.049.595625
AACTTTG166200.045.4861682
TAACTTT170700.043.875281
ATTCGGG9657.520606E-443.54905394-95
CTCGCTA48654.9403945E-543.1909994-95
TAGTTGA99054.0017767E-1142.42790294-95
ATCCTAA41650.001198950140.35998594-95
GTTAAGA43900.001552810338.2914294-95
TCTATAG22500.037.35540894-95
Produced by FastQC (version 0.11.2)