FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n02_30112.34200000035b51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n02_30112.34200000035b51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12181520
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT214930.17643939344186932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134100.11008478416486613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA10602.8860223E-4116.9869194-95
CTGCCTA11954.649602E-4103.7708294-95
GTAAATA16951.52434895E-597.5466194-95
CTTTGTG1601900.062.004071
TTTGTGT1753300.056.7264982
TTGTGTT1888750.053.7756083
TGTGTTT2027350.050.334674
ACTTTGT472400.048.880651
TAACTTT249450.048.5486221
AACTTTG257200.047.431122
GTGTTTG2200100.046.3824355
ACCAACG56651.3339234E-436.48312494-95
TTGAGGG358650.034.9381189
TTTGAGG650200.034.875258
GTTTGAG1703400.034.4272737
TGTTTGA2985450.034.160876
AGGATCG12656.5847416E-1032.67618694-95
GTTTGAC587800.032.1142167
TGACACG13652.5465852E-1130.28232894-95
ACTAACG27450.030.1168594-95
Produced by FastQC (version 0.11.2)