##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30427.34100000035d1e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22972646 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7438004747037 33.0 33.0 33.0 33.0 33.0 2 32.75747952586742 33.0 33.0 33.0 33.0 33.0 3 32.79162352477812 33.0 33.0 33.0 33.0 33.0 4 36.6532087770821 37.0 37.0 37.0 37.0 37.0 5 36.693931295506836 37.0 37.0 37.0 37.0 37.0 6 36.67464435746757 37.0 37.0 37.0 37.0 37.0 7 36.646778956155075 37.0 37.0 37.0 37.0 37.0 8 36.639757736222464 37.0 37.0 37.0 37.0 37.0 9 36.62779233180192 37.0 37.0 37.0 37.0 37.0 10-11 36.59832580887722 37.0 37.0 37.0 37.0 37.0 12-13 36.585844921825725 37.0 37.0 37.0 37.0 37.0 14-15 38.71288612117211 40.0 37.0 40.0 37.0 40.0 16-17 38.66743376013368 40.0 37.0 40.0 37.0 40.0 18-19 38.63076826239346 40.0 37.0 40.0 37.0 40.0 20-21 38.59298260635714 40.0 37.0 40.0 37.0 40.0 22-23 38.54523910741497 40.0 37.0 40.0 37.0 40.0 24-25 38.49198868950491 40.0 37.0 40.0 37.0 40.0 26-27 38.4148358878642 40.0 37.0 40.0 37.0 40.0 28-29 38.3350386368205 40.0 37.0 40.0 37.0 40.0 30-31 38.28056228263823 40.0 37.0 40.0 37.0 40.0 32-33 38.203220625956625 40.0 37.0 40.0 37.0 40.0 34-35 38.1171333506815 40.0 37.0 40.0 37.0 40.0 36-37 38.01444467911968 40.0 37.0 40.0 37.0 40.0 38-39 37.89079984517238 40.0 37.0 40.0 33.0 40.0 40-41 37.763390294700926 40.0 37.0 40.0 33.0 40.0 42-43 37.59498361660211 40.0 37.0 40.0 33.0 40.0 44-45 37.445008642017115 40.0 37.0 40.0 33.0 40.0 46-47 37.21647079748672 37.0 37.0 40.0 33.0 40.0 48-49 36.98013657199088 37.0 37.0 40.0 33.0 40.0 50-51 36.75659719389748 37.0 37.0 40.0 33.0 40.0 52-53 36.487842018720876 37.0 37.0 40.0 33.0 40.0 54-55 36.20511903591776 37.0 37.0 40.0 33.0 40.0 56-57 35.91543416461474 37.0 37.0 38.5 33.0 40.0 58-59 35.611633918879 37.0 33.0 37.0 33.0 40.0 60-61 35.315611488550346 37.0 33.0 37.0 33.0 40.0 62-63 35.0663789230026 37.0 33.0 37.0 33.0 40.0 64-65 34.7924304409688 37.0 33.0 37.0 30.0 38.5 66-67 34.52304873805133 37.0 33.0 37.0 27.0 37.0 68-69 34.26102045450054 37.0 33.0 37.0 27.0 37.0 70-71 34.058573204845445 37.0 33.0 37.0 27.0 37.0 72-73 33.904739227688445 37.0 33.0 37.0 27.0 37.0 74-75 33.67168740596969 37.0 33.0 37.0 27.0 37.0 76-77 30.673240949257654 33.0 30.0 33.0 24.5 35.0 78-79 33.46494086924075 35.0 33.0 37.0 27.0 37.0 80-81 34.01592108719213 37.0 33.0 37.0 27.0 37.0 82-83 34.013166397984804 37.0 33.0 37.0 27.0 37.0 84-85 33.94172121052142 37.0 33.0 37.0 27.0 37.0 86-87 33.8088902558286 37.0 33.0 37.0 27.0 37.0 88-89 33.693576308101385 37.0 33.0 37.0 27.0 37.0 90-91 33.60976817820638 37.0 33.0 37.0 27.0 37.0 92-93 33.51257138598662 37.0 33.0 37.0 27.0 37.0 94-95 33.381881325294444 37.0 33.0 37.0 27.0 37.0 96-97 33.27660805812269 37.0 33.0 37.0 27.0 37.0 98-99 33.11181171729194 33.0 33.0 37.0 27.0 37.0 100-101 31.16674716094959 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08764173706529022 2306 2 0.10260920608100577 2306 3 0.07103259401676354 2306 4 0.017678748554338597 2306 5 0.041644250688605666 2306 6 0.07774441859120884 2306 7 0.08696843749390837 2306 8 0.10963783982115416 2306 9 0.09627370436894012 2306 10-11 0.11340340313812902 2306 12-13 0.08830771946330174 2306 14-15 0.15151474192073522 2306 16-17 0.1911828736825214 2306 18-19 0.16267728474453946 2306 20-21 0.11959703911575303 2306 22-23 0.1287855389192032 2306 24-25 0.16192231956208047 2306 26-27 0.18844334745772073 2306 28-29 0.14052588433948898 2306 30-31 0.14711399121883062 2306 32-33 0.15283505935891384 2306 34-35 0.1527441629799 2306 36-37 0.12791111029907398 2306 38-39 0.14455445736649608 2306 40-41 0.09608580627997299 2306 42-43 0.10635587285829473 2306 44-45 0.12365513170122 2306 46-47 0.14391113097249786 2306 48-49 0.051091080568731684 2306 50-51 0.024172990096488434 2306 52-53 0.035221489891632984 2306 54-55 6.61495052867167E-4 2306 56-57 0.08448137850075454 2306 58-59 -0.021494318395568257 2306 60-61 0.005368476697157121 2306 62-63 0.01679891387175303 2306 64-65 0.008846865257744696 2306 66-67 -0.12429908248276433 2306 68-69 -0.1693620938203395 2306 70-71 -0.20737328833217106 2306 72-73 -0.19841261341034766 2306 74-75 -0.26619884355142176 2306 76-77 -0.5728972166777311 2306 78-79 -0.09854703793824626 2306 80-81 2.0952205850477412E-4 2306 82-83 -0.026076465407633975 2306 84-85 -0.07386700404925506 2306 86-87 -0.13046722560632418 2306 88-89 -0.02390214686383274 2306 90-91 -0.12595815525585152 2306 92-93 -0.19765338221108664 2306 94-95 -0.1601196600997028 2306 96-97 -0.30101808600986146 2306 98-99 -0.1821791627322682 2306 100-101 -0.3574276515814141 2307 1 0.09240418595815214 2307 2 0.0832651527257866 2307 3 0.07220516368091268 2307 4 6.755758506145071E-4 2307 5 0.0012445055504812785 2307 6 0.08449992472902323 2307 7 0.10005249469361388 2307 8 0.11679911953731192 2307 9 0.06156100252028551 2307 10-11 0.09300708773830735 2307 12-13 0.09826816310400233 2307 14-15 0.14464644378389124 2307 16-17 0.16196605018472354 2307 18-19 0.13017778366181432 2307 20-21 0.12469799162615658 2307 22-23 0.1310215085712514 2307 24-25 0.08971710654238763 2307 26-27 0.10072126965657446 2307 28-29 0.08578961144902308 2307 30-31 0.10239951593548113 2307 32-33 0.04356410143575573 2307 34-35 0.11797201797505608 2307 36-37 0.140812521661978 2307 38-39 0.06927222973280323 2307 40-41 0.07946753334466905 2307 42-43 0.11129259708528849 2307 44-45 0.07750771307246396 2307 46-47 0.0644574979472381 2307 48-49 -0.0184410505340864 2307 50-51 0.03761112060814753 2307 52-53 -0.07071794678032006 2307 54-55 -0.006918867480735003 2307 56-57 -0.03759951839364817 2307 58-59 -0.036117441460248756 2307 60-61 -0.07679237584015652 2307 62-63 -0.04740813349779671 2307 64-65 -0.12583480244526157 2307 66-67 -0.24429623294548009 2307 68-69 -0.21292008835684584 2307 70-71 -0.2483459863363251 2307 72-73 -0.32485848094824377 2307 74-75 -0.3909650319681006 2307 76-77 -0.6847957397242119 2307 78-79 -0.20359350036434876 2307 80-81 -0.1087819365987528 2307 82-83 -0.2106591557626416 2307 84-85 -0.17153824943799378 2307 86-87 -0.12642028686226325 2307 88-89 -0.17607106560772223 2307 90-91 -0.26718115052037916 2307 92-93 -0.2346317752898841 2307 94-95 -0.24978545323529744 2307 96-97 -0.4947692844401388 2307 98-99 -0.30458034673672074 2307 100-101 -0.3557553062652694 2201 1 0.08349555496307204 2201 2 0.0872315845964664 2201 3 0.06458416915675258 2201 4 0.017912270735756408 2201 5 0.04350690860443507 2201 6 0.07247742032558335 2201 7 0.10522096197379227 2201 8 0.11397978010301557 2201 9 0.09333410352099492 2201 10-11 0.08424909168333983 2201 12-13 0.07797869887085085 2201 14-15 0.17278588287073404 2201 16-17 0.15085136458152704 2201 18-19 0.18344949910603958 2201 20-21 0.14500073012824544 2201 22-23 0.12126887501005257 2201 24-25 0.1707561913140907 2201 26-27 0.15339722496513986 2201 28-29 0.1605112161232185 2201 30-31 0.16370064203002954 2201 32-33 0.15464904056486972 2201 34-35 0.1688003368904205 2201 36-37 0.15946024477997156 2201 38-39 0.13993509471447396 2201 40-41 0.12394588436892917 2201 42-43 0.16170099378093994 2201 44-45 0.12352301329472226 2201 46-47 0.11448560578262601 2201 48-49 0.13156640591973456 2201 50-51 0.10052585083672483 2201 52-53 0.0604772199479342 2201 54-55 0.07252434672528807 2201 56-57 0.11389360693404171 2201 58-59 0.03314433398860217 2201 60-61 0.0244292374158519 2201 62-63 0.05658456516852084 2201 64-65 0.00734324504222883 2201 66-67 -0.07793253578793724 2201 68-69 -0.03102638710918626 2201 70-71 -0.12173966216242604 2201 72-73 -0.14506299666087585 2201 74-75 -0.0740846794729535 2201 76-77 -0.05713150748312046 2201 78-79 0.03753787765632666 2201 80-81 0.006072069111866085 2201 82-83 -0.01676799278576624 2201 84-85 0.03621154824194406 2201 86-87 0.059673266454545626 2201 88-89 0.08925396939146424 2201 90-91 0.020927797256597103 2201 92-93 -0.02195832003881293 2201 94-95 0.0337021431440192 2201 96-97 -0.13525960983298546 2201 98-99 -0.049290642860761125 2201 100-101 -0.059843157702268 1101 1 0.0818229698432873 1101 2 0.08032440366322646 1101 3 0.0625271278358639 1101 4 0.011369418567817036 1101 5 0.015613717078217348 1101 6 0.06290257494510598 1101 7 0.04719327086579739 1101 8 0.04957310040766316 1101 9 0.057082457827398514 1101 10-11 0.05352069788328606 1101 12-13 0.07718021717959545 1101 14-15 0.08159349713124442 1101 16-17 0.05134231098158182 1101 18-19 0.06135828220235595 1101 20-21 0.054821736506028174 1101 22-23 0.0368701671698517 1101 24-25 0.055354135594420484 1101 26-27 0.04510856049363099 1101 28-29 0.040275737711517934 1101 30-31 -2.969840953568337E-4 1101 32-33 -0.0062858887178904865 1101 34-35 0.03181229557224441 1101 36-37 0.00884436052890436 1101 38-39 0.00531928868349496 1101 40-41 -0.048791806532271664 1101 42-43 0.056695912472513044 1101 44-45 0.0952020448281985 1101 46-47 0.05769533989727904 1101 48-49 0.016087153571866963 1101 50-51 4.960142075560725E-5 1101 52-53 -0.07459470506628207 1101 54-55 -0.053819054721458315 1101 56-57 -0.045639357735950625 1101 58-59 -0.0025258203147444647 1101 60-61 0.05553352666353106 1101 62-63 -0.05220364691518853 1101 64-65 -0.058232397823452686 1101 66-67 -0.14840821129027404 1101 68-69 -0.08389731438278858 1101 70-71 -0.1677522331186836 1101 72-73 -0.2846547627534619 1101 74-75 -0.2631720578984087 1101 76-77 -0.5138039457529402 1101 78-79 -0.05632482032514474 1101 80-81 0.08623705504573564 1101 82-83 0.022987680342993144 1101 84-85 -0.05848686089344568 1101 86-87 -0.06238254312480507 1101 88-89 0.007321978306755739 1101 90-91 -0.026912092228322138 1101 92-93 -0.1110584054815078 1101 94-95 -0.15642311687781074 1101 96-97 -0.2259935120051395 1101 98-99 -0.15078110405655565 1101 100-101 -0.10210752270054968 2304 1 0.08220878889699179 2304 2 0.08603967035620741 2304 3 0.06205896474666872 2304 4 -0.005823612482267038 2304 5 0.00256118826702334 2304 6 0.02224807175552712 2304 7 0.05597637745346162 2304 8 0.06598958901196283 2304 9 0.03348887940985179 2304 10-11 0.0629522207299189 2304 12-13 0.03881963013911616 2304 14-15 0.0632799413357219 2304 16-17 0.046355406536463306 2304 18-19 0.05416599227078933 2304 20-21 0.038537498506649115 2304 22-23 0.06869363753364155 2304 24-25 0.0015856592004368508 2304 26-27 0.01631873981955323 2304 28-29 0.021673422688387234 2304 30-31 0.06406367802839696 2304 32-33 0.044153328783075096 2304 34-35 0.033653474955094964 2304 36-37 0.04468272572635357 2304 38-39 0.08090142948454115 2304 40-41 -0.06304879033231003 2304 42-43 -0.04583460455788213 2304 44-45 0.019945912079357697 2304 46-47 0.012095476199583288 2304 48-49 0.019324259752657724 2304 50-51 -0.04691435805928279 2304 52-53 -0.09626793109354281 2304 54-55 -0.055285898751790796 2304 56-57 -0.020621757350617997 2304 58-59 -0.04450966148847613 2304 60-61 -0.04100896668899168 2304 62-63 -0.09875884491879106 2304 64-65 -0.09931879503509577 2304 66-67 -0.1624433216568164 2304 68-69 -0.14116109774405317 2304 70-71 -0.1751286494538533 2304 72-73 -0.16896204374918966 2304 74-75 -0.21420857995946818 2304 76-77 -0.4647153988590489 2304 78-79 -0.22294105816261123 2304 80-81 0.002970533492721472 2304 82-83 0.01608038881362006 2304 84-85 -9.90247745697559E-4 2304 86-87 0.007370057401303143 2304 88-89 0.07674415354551911 2304 90-91 0.03840199742123218 2304 92-93 -0.12133605437045247 2304 94-95 -0.1622780309282703 2304 96-97 -0.18002599501186722 2304 98-99 -0.13533709412606498 2304 100-101 0.04658817531042203 2202 1 0.0665105332901561 2202 2 0.07328051007998937 2202 3 0.048207173940298276 2202 4 -0.034279555852066323 2202 5 -0.01849943635243534 2202 6 0.026132019475873847 2202 7 0.018111030726082333 2202 8 0.048538528670846404 2202 9 0.03364704480780034 2202 10-11 0.025491869112876486 2202 12-13 0.020213473097108192 2202 14-15 0.0586958469725829 2202 16-17 0.008577480325229203 2202 18-19 -0.025522242443933862 2202 20-21 -0.033677385819693484 2202 22-23 0.05737658555059966 2202 24-25 0.001203265755087557 2202 26-27 0.011843826017852166 2202 28-29 -0.019106152398457255 2202 30-31 0.027157099326437617 2202 32-33 0.005346340670008942 2202 34-35 0.014946670475993074 2202 36-37 0.04531966094737072 2202 38-39 0.0637262328081647 2202 40-41 0.010017150116517826 2202 42-43 0.05085913959729993 2202 44-45 0.03725678427634449 2202 46-47 0.08376286313852432 2202 48-49 0.049020052854771734 2202 50-51 -0.011393505555005845 2202 52-53 0.009322321808269862 2202 54-55 0.02518609720193865 2202 56-57 0.010502180867106858 2202 58-59 -0.02856733231317321 2202 60-61 0.04952828822517574 2202 62-63 0.05009176186357678 2202 64-65 -0.008743622262258555 2202 66-67 -0.11105477429848776 2202 68-69 -0.06540733619996075 2202 70-71 -0.016549434161866827 2202 72-73 -0.16766958186369862 2202 74-75 -0.15799649550527306 2202 76-77 -0.38974409050990033 2202 78-79 -0.07075405501376508 2202 80-81 0.1420991246672827 2202 82-83 0.06971304379582222 2202 84-85 0.12061385530223134 2202 86-87 0.08395996105277703 2202 88-89 0.0823345616651352 2202 90-91 0.03422916855644331 2202 92-93 0.09493126644570538 2202 94-95 -0.031018112407956266 2202 96-97 -0.14268524333667187 2202 98-99 0.03933318188901325 2202 100-101 0.12201438489088545 1102 1 0.06569381501884664 1102 2 0.0711851835909485 1102 3 0.050557469321830695 1102 4 -0.047027083877566156 1102 5 -0.05242428312622138 1102 6 0.0313475489504782 1102 7 0.019663968023508005 1102 8 0.03962381666892867 1102 9 0.013868826687279068 1102 10-11 0.058145037965310564 1102 12-13 0.05322576475482066 1102 14-15 0.07274522470142841 1102 16-17 0.048105454502767486 1102 18-19 0.04629930879546862 1102 20-21 0.031209855954905663 1102 22-23 0.054204657644419285 1102 24-25 0.008193094765694298 1102 26-27 0.04307575220087756 1102 28-29 0.03987395146746309 1102 30-31 -0.05856909366115559 1102 32-33 0.004777057844115973 1102 34-35 0.044708901280195334 1102 36-37 -0.027165670683196197 1102 38-39 -0.026779416456705007 1102 40-41 -0.027551221911465973 1102 42-43 -0.04508593983778297 1102 44-45 -0.03613412586904019 1102 46-47 -0.006296617278835015 1102 48-49 -0.0035069940545682243 1102 50-51 -0.03081508352396156 1102 52-53 -0.03870558904200294 1102 54-55 -0.11872591668612387 1102 56-57 -0.05611926517047294 1102 58-59 -0.07286999692770735 1102 60-61 -0.04919305345283931 1102 62-63 -0.06369574730885574 1102 64-65 -0.12140324914882683 1102 66-67 -0.19183673095355402 1102 68-69 -0.1865443374118314 1102 70-71 -0.2203898152148085 1102 72-73 -0.18063621862382462 1102 74-75 -0.20874767444936992 1102 76-77 -0.4969701287644135 1102 78-79 -0.12393913457317751 1102 80-81 0.034332473428690946 1102 82-83 -0.03978119367045707 1102 84-85 -0.03634240469201444 1102 86-87 -0.020627769056119405 1102 88-89 -0.11109422510858735 1102 90-91 -0.17870916238944545 1102 92-93 -0.23461900367188804 1102 94-95 -0.33838425686794693 1102 96-97 -0.3843077765296101 1102 98-99 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48-49 -0.2219567166522225 1213 50-51 -0.14022863141549635 1213 52-53 -0.1258897412873452 1213 54-55 -0.09427963180493748 1213 56-57 -0.09758631550910479 1213 58-59 -0.099980280791101 1213 60-61 -0.072501482560682 1213 62-63 -0.04561473081997747 1213 64-65 0.04834900367860229 1213 66-67 0.055565635365638855 1213 68-69 0.07379422258201629 1213 70-71 0.06420751125177304 1213 72-73 0.08984969007805432 1213 74-75 0.17870370918122092 1213 76-77 0.5464397774397654 1213 78-79 0.06205952799772518 1213 80-81 -0.0947137869850323 1213 82-83 -0.02789051096483064 1213 84-85 -0.021863262250541027 1213 86-87 -0.03037863409924313 1213 88-89 0.03599526537528419 1213 90-91 0.04589577699346137 1213 92-93 0.08445704407517951 1213 94-95 0.19720697391100117 1213 96-97 0.10006892942499235 1213 98-99 0.2558824953956176 1213 100-101 0.5650616868282476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 51.0 8 629.0 9 1871.0 10 1260.0 11 423.0 12 329.0 13 628.0 14 1457.0 15 3272.0 16 5861.0 17 9399.0 18 13210.0 19 17879.0 20 24384.0 21 32279.0 22 41860.0 23 55299.0 24 71595.0 25 92493.0 26 119445.0 27 154787.0 28 199930.0 29 259706.0 30 339390.0 31 450840.0 32 620468.0 33 911186.0 34 1496232.0 35 2896879.0 36 6282002.0 37 7855442.0 38 1012110.0 39 47.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.63244310433602 24.584791509123235 27.458344456182886 24.324420930357856 2 26.00379163984854 24.456007375032026 26.744524770894916 22.795676214224518 3 24.110775049595944 23.133347373219436 28.715151053997 24.04072652318762 4 24.412579104781187 22.74254445859435 28.10049705499126 24.744379381633202 5 26.79565079268622 22.13456386347485 27.41638468637875 23.653400657460182 6 27.22335946847394 22.404184524499268 27.113019545071126 23.259436461955666 7 26.470133218437265 23.908403933965637 26.30866292024001 23.312799927357084 8 26.69205802413879 23.09987713213358 26.561137101925482 23.64692774180214 9 24.774677675353548 22.76389929135721 28.719721707286133 23.74170132600311 10-11 26.272565341435104 23.33216403291851 26.607225257951107 23.788045367695283 12-13 26.202721706502597 24.470415815400628 25.23533423185122 24.091528246245556 14-15 26.28270190573452 23.864300818192298 25.275523050167713 24.577474225905473 16-17 26.09803415766734 23.558215714463195 25.11736131745555 25.226388810413912 18-19 26.03819342360475 23.732222661682073 25.24042289251312 24.989161022200054 20-21 25.560156316861864 23.980626334843038 25.379086499178428 25.08013084911667 22-23 25.603657062403695 24.117076021630247 25.133404310500413 25.145862605465645 24-25 25.752780067215593 24.086324231000646 24.94737001562641 25.213525686157357 26-27 25.631176748207412 24.16059734694906 25.105214262214286 25.103011642629237 28-29 25.722023923582853 24.266259968485997 24.831175303010372 25.180540804920774 30-31 25.814423299093757 24.15416324012621 24.808441660765443 25.222971800014594 32-33 25.74309463524576 24.490365628756912 24.647800693050335 25.11873904294699 34-35 25.617793508206276 24.450024231037293 24.74389166956831 25.18829059118812 36-37 25.737518427118957 24.36601906824155 24.697202804097603 25.199259700541894 38-39 25.743205933318603 24.317219436908687 24.686040572762856 25.25353405700986 40-41 25.629949790513923 24.442088222344847 24.69183944518225 25.236122541958977 42-43 25.590718413554274 24.374579087460962 24.691413512618553 25.343288986366208 44-45 25.80625677852498 24.256488176119436 24.610184889540108 25.327070155815477 46-47 25.676445673797687 24.241815138647333 24.637820710872465 25.443918476682516 48-49 25.73627468048874 24.25902733668818 24.60099956794229 25.403698414880786 50-51 25.531031271712173 24.434616670605447 24.83105069064765 25.203301367034726 52-53 25.58057798448918 24.376657153374243 24.89072804366816 25.15203681846841 54-55 25.3789939831216 24.355963347408643 24.824877876614472 25.440164792855285 56-57 25.53604132559372 24.508629102347864 24.772382623032637 25.18294694902577 58-59 25.335759466439427 24.469564450941462 24.908367872930977 25.286308209688137 60-61 25.619912195436477 24.52909791182533 24.505616343920096 25.345373548818095 62-63 25.42685224288597 24.604815535165642 24.736033469486244 25.232298752462146 64-65 25.537578061118342 24.648988071582433 24.700091763755626 25.113342103543594 66-67 25.663367082347243 24.61167954457987 24.611494517091316 25.11345885598157 68-69 25.596031826913784 24.604376084168994 24.771879969090875 25.027712119826344 70-71 25.49976015291528 24.771039471818778 24.64397069139805 25.085229683867894 72-73 25.474595086085277 24.628418062385386 24.671658310651416 25.22532854087793 74-75 25.365073923809856 24.660705731729717 24.666243470790146 25.307976873670285 76-77 25.464433912556224 24.59737275979522 24.552534866001512 25.385658461647044 78-79 25.335385073096926 24.54811838588355 24.600579323207544 25.515917217811975 80-81 25.329998383400643 24.558109738037988 24.59679537202017 25.515096506541195 82-83 25.18974407862898 24.796187825781104 24.628556895472645 25.38551120011727 84-85 25.387277871392435 24.71716430346649 24.491963755181263 25.403594069959812 86-87 25.234673324962575 24.80869980647303 24.588236047003363 25.368390821561032 88-89 25.2563684927778 25.296101405236413 24.30676429270877 25.140765809277017 90-91 25.401873352317892 24.941096681444204 24.31769256599542 25.339337400242485 92-93 25.23376170049553 25.08849127075407 24.259889606579087 25.417857422171313 94-95 25.378568340901296 25.14450475038555 24.03501211096037 25.441914797752784 96-97 25.277701017328674 24.992885824886073 24.203443702703744 25.52596945508151 98-99 25.420962436862983 24.98785866365396 24.199158412597292 25.39202048688577 100-101 25.600961798076995 25.12077403249919 23.891768610875914 25.386495558547896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4195.0 1 2735.0 2 1325.5 3 1405.0 4 1523.0 5 1481.0 6 1366.0 7 1372.0 8 1492.5 9 1601.5 10 1680.5 11 1820.0 12 1921.5 13 2053.5 14 2163.5 15 2275.5 16 2429.0 17 2580.5 18 2810.5 19 3035.0 20 3287.5 21 3697.5 22 4226.0 23 4842.5 24 5480.0 25 6337.0 26 7400.0 27 8648.0 28 10180.5 29 12247.5 30 15173.0 31 18838.5 32 24152.0 33 30834.5 34 39138.5 35 50968.5 36 65637.5 37 85661.5 38 112490.0 39 146807.5 40 199858.0 41 291647.0 42 383008.5 43 438173.5 44 551180.0 45 711676.5 46 904994.5 47 1206134.0 48 1571644.5 49 1758943.5 50 1686260.0 51 1559551.0 52 1478630.5 53 1483394.5 54 1438297.0 55 1307061.0 56 1217190.5 57 1045519.5 58 808600.5 59 642986.0 60 520238.5 61 388182.0 62 257682.0 63 160754.0 64 91440.5 65 50297.0 66 30491.0 67 21020.0 68 16195.5 69 12906.5 70 10468.5 71 8335.0 72 6473.5 73 4953.5 74 3796.5 75 2851.0 76 2040.5 77 1446.5 78 991.5 79 662.5 80 469.0 81 305.0 82 182.5 83 121.0 84 86.5 85 54.0 86 40.5 87 37.0 88 33.5 89 31.0 90 24.0 91 13.5 92 11.5 93 12.0 94 11.5 95 9.0 96 5.0 97 3.5 98 2.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1387824458706237 2 0.0 3 0.0 4 0.18275648351522067 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.598371907180391E-4 12-13 0.0 14-15 4.897128524071629E-4 16-17 0.0 18-19 0.0 20-21 0.0036739346438368486 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.3530031325081145E-5 32-33 0.0 34-35 1.871791346978489E-4 36-37 2.1982665819165976E-4 38-39 0.009411192772482544 40-41 0.007780993099358254 42-43 0.0010316617424044232 44-45 0.00122319388023478 46-47 0.0021525600490252626 48-49 8.923656421641634E-5 50-51 9.707196985493094E-4 52-53 0.020056461933031136 54-55 0.01817161157665512 56-57 0.021723662132781745 58-59 0.041919420166053135 60-61 0.015524985672090189 62-63 0.0024267992463732737 64-65 0.007413164334661319 66-67 0.01343336766692004 68-69 0.028779880210577397 70-71 0.01219929127885399 72-73 0.019420923475684953 74-75 0.012985008344271704 76-77 0.0237913386207231 78-79 0.009670196458866775 80-81 0.006906039469724123 82-83 0.00197408692059243 84-85 0.020343760139776672 86-87 0.009594018904047883 88-89 0.013161304971138283 90-91 0.0463790718753077 92-93 0.03584262779307181 94-95 0.029942132046957066 96-97 0.02687761784167135 98-99 0.03356600715477007 100-101 0.24370505687503302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2972646E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.635393711463646 #Duplication Level Percentage of deduplicated Percentage of total 1 80.91216468676951 15.887422096715047 2 8.477292737223253 3.329099610054197 3 2.9719874460418043 1.7506843082567443 4 1.0928502820633548 0.8583418222399226 5 1.0609951081070688 1.0416528336809612 6 0.6316648222236219 0.7441792486825504 7 0.4748801181191708 0.6527120659511394 8 0.39372548410126706 0.6184763915651998 9 0.2917039655255837 0.5154949989261048 >10 2.395134692565224 10.450112060300052 >50 0.5527813629322732 7.688663850084564 >100 0.5994309099503752 25.87843315705298 >500 0.09454356267355211 12.633145697537303 >1k 0.05051597830682501 17.59499791424053 >5k 3.288433976928422E-4 0.35658394471262644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1317808144521097E-4 0.0 4.353003132508114E-6 2 1.1317808144521097E-4 0.0 4.353003132508114E-6 3 1.1317808144521097E-4 0.0 4.353003132508114E-6 4 1.1317808144521097E-4 0.0 4.353003132508114E-6 5 1.218840877102272E-4 0.0 4.353003132508114E-6 6 1.2623709084273532E-4 0.0 4.353003132508114E-6 7 1.2623709084273532E-4 0.0 4.353003132508114E-6 8 1.52355109637784E-4 0.0 4.353003132508114E-6 9 1.6976712216781645E-4 0.0 4.353003132508114E-6 10-11 2.0241464566162732E-4 0.0 4.353003132508114E-6 12-13 2.960042130105518E-4 0.0 4.353003132508114E-6 14-15 4.157117991545249E-4 0.0 4.353003132508114E-6 16-17 5.985379307198657E-4 0.0 4.353003132508114E-6 18-19 8.248940936102876E-4 0.0 4.353003132508114E-6 20-21 0.0010033672220431202 0.0 4.353003132508114E-6 22-23 0.0012493118990298288 0.0 4.353003132508114E-6 24-25 0.0015431396104741263 0.0 4.353003132508114E-6 26-27 0.0018565558360147108 0.0 4.353003132508114E-6 28-29 0.002257032124205457 0.0 4.353003132508114E-6 30-31 0.0026683909202274737 0.0 4.353003132508114E-6 32-33 0.0032560463431160697 0.0 4.353003132508114E-6 34-35 0.0039786448631124165 0.0 4.353003132508114E-6 36-37 0.004829656975517753 0.0 4.353003132508114E-6 38-39 0.005891789739849732 2.176501566254057E-6 4.353003132508114E-6 40-41 0.007175925663939627 4.353003132508114E-6 1.0882507831270286E-5 42-43 0.008875773387184045 4.353003132508114E-6 1.3059009397524342E-5 44-45 0.010867272320306508 4.353003132508114E-6 1.3059009397524342E-5 46-47 0.01327230655101724 4.353003132508114E-6 1.3059009397524342E-5 48-49 0.016075640568352464 6.529504698762171E-6 1.3059009397524342E-5 50-51 0.019383922949058635 1.0882507831270286E-5 1.52355109637784E-5 52-53 0.023480098896748767 1.3059009397524342E-5 1.7412012530032457E-5 54-55 0.02852305302575942 1.3059009397524342E-5 1.7412012530032457E-5 56-57 0.0346542579378971 1.3059009397524342E-5 1.7412012530032457E-5 58-59 0.041793183075210405 1.3059009397524342E-5 1.7412012530032457E-5 60-61 0.05037730525251641 1.3059009397524342E-5 1.7412012530032457E-5 62-63 0.06089633732222226 1.3059009397524342E-5 1.9588514096286513E-5 64-65 0.07401193576046922 1.3059009397524342E-5 2.6118018795048684E-5 66-67 0.09011151784605047 1.3059009397524342E-5 2.8294520361302744E-5 68-69 0.10938879221836266 1.3059009397524342E-5 3.04710219275568E-5 70-71 0.1335240180865539 1.3059009397524342E-5 3.04710219275568E-5 72-73 0.16306567384532022 1.3059009397524342E-5 3.4824025060064915E-5 74-75 0.19942848551272674 1.7412012530032457E-5 3.4824025060064915E-5 76-77 0.24411206266792254 1.7412012530032457E-5 3.4824025060064915E-5 78-79 0.2984005412349975 1.7412012530032457E-5 3.4824025060064915E-5 80-81 0.3648883110809264 1.7412012530032457E-5 3.4824025060064915E-5 82-83 0.4459325234019625 2.1765015662540572E-5 3.4824025060064915E-5 84-85 0.5440296254945991 2.1765015662540572E-5 3.4824025060064915E-5 86-87 0.6619981868871352 2.1765015662540572E-5 3.4824025060064915E-5 88-89 0.8040736796275013 2.1765015662540572E-5 3.4824025060064915E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACTTT 24250 0.0 64.50677 1 AACTTTG 25590 0.0 61.397057 2 ACTTTGT 49560 0.0 45.58644 1 CTTTGTG 93335 0.0 41.307842 1 TCCGATC 1790 0.0 38.99315 7 TTTGTGT 99795 0.0 38.737423 2 TTGTGTT 132330 0.0 30.500208 3 TGTGTTT 136150 0.0 29.759747 4 GTGTTTG 153340 0.0 26.530655 5 TGTTTGA 173680 0.0 23.325182 6 GTTTGAG 97715 0.0 23.04222 7 TTTGAGG 38500 0.0 22.297783 8 TTGAGGG 22690 0.0 18.101244 9 GTTTGAC 36770 0.0 18.078333 7 TTTGAGC 32420 0.0 16.90117 8 TTGAGGT 12650 0.0 16.47786 9 TTTGATG 20185 0.0 16.183918 8 TTTGAGT 41840 0.0 15.592618 8 TTTGACC 11125 0.0 15.364784 8 TTGAGGC 8975 0.0 15.342296 9 >>END_MODULE