##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30348.34100000035cf3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16874426 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75165211545566 33.0 33.0 33.0 33.0 33.0 2 32.767939543543584 33.0 33.0 33.0 33.0 33.0 3 32.800772541833425 33.0 33.0 33.0 33.0 33.0 4 36.672955749724466 37.0 37.0 37.0 37.0 37.0 5 36.71267467112659 37.0 37.0 37.0 37.0 37.0 6 36.68193489959303 37.0 37.0 37.0 37.0 37.0 7 36.65492598088966 37.0 37.0 37.0 37.0 37.0 8 36.65069585181742 37.0 37.0 37.0 37.0 37.0 9 36.639038566408125 37.0 37.0 37.0 37.0 37.0 10-11 36.61516338392784 37.0 37.0 37.0 37.0 37.0 12-13 36.59540564520535 37.0 37.0 37.0 37.0 37.0 14-15 38.723462978829616 40.0 37.0 40.0 37.0 40.0 16-17 38.69190163860981 40.0 37.0 40.0 37.0 40.0 18-19 38.65450389245832 40.0 37.0 40.0 37.0 40.0 20-21 38.61274211638369 40.0 37.0 40.0 37.0 40.0 22-23 38.55998266252138 40.0 37.0 40.0 37.0 40.0 24-25 38.50803325102732 40.0 37.0 40.0 37.0 40.0 26-27 38.43529483610287 40.0 37.0 40.0 37.0 40.0 28-29 38.36059828049855 40.0 37.0 40.0 37.0 40.0 30-31 38.31508843619332 40.0 37.0 40.0 37.0 40.0 32-33 38.24088608999204 40.0 37.0 40.0 37.0 40.0 34-35 38.15313303101391 40.0 37.0 40.0 37.0 40.0 36-37 38.04263066488899 40.0 37.0 40.0 37.0 40.0 38-39 37.93504235344064 40.0 37.0 40.0 33.0 40.0 40-41 37.82814073201661 40.0 37.0 40.0 33.0 40.0 42-43 37.674266935064935 40.0 37.0 40.0 33.0 40.0 44-45 37.54964050332734 40.0 37.0 40.0 33.0 40.0 46-47 37.356326135182314 38.5 37.0 40.0 33.0 40.0 48-49 37.153443115635454 37.0 37.0 40.0 33.0 40.0 50-51 36.95328421837875 37.0 37.0 40.0 33.0 40.0 52-53 36.70230860000808 37.0 37.0 40.0 33.0 40.0 54-55 36.43905875672452 37.0 37.0 40.0 33.0 40.0 56-57 36.179681993331215 37.0 37.0 40.0 33.0 40.0 58-59 35.897898364068794 37.0 37.0 38.5 33.0 40.0 60-61 35.605894979775904 37.0 33.0 37.0 33.0 40.0 62-63 35.36403045650263 37.0 33.0 37.0 33.0 40.0 64-65 35.08255972677234 37.0 33.0 37.0 33.0 40.0 66-67 34.80153890864199 37.0 33.0 37.0 30.0 37.0 68-69 34.521427573299384 37.0 33.0 37.0 27.0 37.0 70-71 34.30907972810453 37.0 33.0 37.0 27.0 37.0 72-73 34.14249578030091 37.0 33.0 37.0 27.0 37.0 74-75 33.89857453521678 37.0 33.0 37.0 27.0 37.0 76-77 30.939179442311104 33.0 30.0 33.0 24.5 37.0 78-79 33.68387378035851 35.0 33.0 37.0 27.0 37.0 80-81 34.18592916879069 37.0 33.0 37.0 27.0 37.0 82-83 34.16862138006946 37.0 33.0 37.0 27.0 37.0 84-85 34.093138338453706 37.0 33.0 37.0 27.0 37.0 86-87 33.94787846413264 37.0 33.0 37.0 27.0 37.0 88-89 33.83508339779972 37.0 33.0 37.0 27.0 37.0 90-91 33.744780978031486 37.0 33.0 37.0 27.0 37.0 92-93 33.657638813906914 37.0 33.0 37.0 27.0 37.0 94-95 33.513938311146106 37.0 33.0 37.0 27.0 37.0 96-97 33.43372835318961 37.0 33.0 37.0 27.0 37.0 98-99 33.2684532499061 37.0 33.0 37.0 27.0 37.0 100-101 31.31584031362015 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08882280368710127 2306 2 0.08975097642950658 2306 3 0.06551042618258407 2306 4 5.574048265586384E-4 2306 5 0.01925274718810499 2306 6 0.06635550296402215 2306 7 0.07839590926564455 2306 8 0.07873912448798137 2306 9 0.09292598651282447 2306 10-11 0.1079677913819097 2306 12-13 0.07900588991853397 2306 14-15 0.1169976499534684 2306 16-17 0.13815250386658562 2306 18-19 0.14619816110408124 2306 20-21 0.13644927148973096 2306 22-23 0.09913467949287735 2306 24-25 0.10542686979337645 2306 26-27 0.139160608246506 2306 28-29 0.09194964934112448 2306 30-31 0.11110528527539287 2306 32-33 0.11763668894853652 2306 34-35 0.10886315534455804 2306 36-37 0.05375907247356082 2306 38-39 0.15648505421532377 2306 40-41 0.08258359200413423 2306 42-43 0.10357390578401748 2306 44-45 0.12365118109628526 2306 46-47 0.1529787858180569 2306 48-49 0.04452100170744444 2306 50-51 0.01240196339422539 2306 52-53 7.246815792001371E-4 2306 54-55 0.015123476126980506 2306 56-57 0.06175315681985438 2306 58-59 0.015543947809881331 2306 60-61 -0.009099873145736126 2306 62-63 -0.014281733733433555 2306 64-65 -0.058006383862853284 2306 66-67 -0.12210873216142915 2306 68-69 -0.18120006146400414 2306 70-71 -0.18915709671261283 2306 72-73 -0.19219076369803645 2306 74-75 -0.2603731312871247 2306 76-77 -0.5010373242566217 2306 78-79 -0.11417741177454133 2306 80-81 -0.015109553414951904 2306 82-83 -0.055274894643687844 2306 84-85 -0.09799320957473157 2306 86-87 -0.11888098317855622 2306 88-89 -0.04525453384693634 2306 90-91 -0.13114232556226568 2306 92-93 -0.14749936347236314 2306 94-95 -0.15988404270778034 2306 96-97 -0.2856148753095482 2306 98-99 -0.11194378843870112 2306 100-101 -0.333525813864032 2307 1 0.10084711182796724 2307 2 0.08087926221583785 2307 3 0.07721555725451879 2307 4 -0.011359983003572438 2307 5 0.02540615132814139 2307 6 0.08750248177159392 2307 7 0.08803698028236795 2307 8 0.10577931496069937 2307 9 0.08511084266579161 2307 10-11 0.08581965833877803 2307 12-13 0.12404354712602839 2307 14-15 0.15436384642648449 2307 16-17 0.14186691126300133 2307 18-19 0.15874729092006135 2307 20-21 0.132460664924146 2307 22-23 0.13913962076455988 2307 24-25 0.12598416486332553 2307 26-27 0.10934579468266747 2307 28-29 0.16040712912005262 2307 30-31 0.14227527513523341 2307 32-33 0.13967225320477894 2307 34-35 0.18760455425150724 2307 36-37 0.16064015873049442 2307 38-39 0.07973519160114506 2307 40-41 0.0626292813065632 2307 42-43 0.14535544547236867 2307 44-45 0.1070945433105095 2307 46-47 0.13175056245884065 2307 48-49 0.06189312869877739 2307 50-51 0.07428243194448925 2307 52-53 -0.03630925664104723 2307 54-55 0.04628777934527051 2307 56-57 -0.005195521469168796 2307 58-59 0.04279482071890328 2307 60-61 -0.032525830801930056 2307 62-63 0.04198630584880192 2307 64-65 -0.025255431321774324 2307 66-67 -0.1703900840570256 2307 68-69 -0.14644295528979256 2307 70-71 -0.19082438948233715 2307 72-73 -0.2398245445166225 2307 74-75 -0.3674865492918755 2307 76-77 -0.5545457874486175 2307 78-79 -0.14648199157809927 2307 80-81 -0.0977458819634549 2307 82-83 -0.12152071930346153 2307 84-85 -0.12794203132741444 2307 86-87 -0.14973282643472174 2307 88-89 -0.1601088640355499 2307 90-91 -0.18352893650595092 2307 92-93 -0.14930138003178683 2307 94-95 -0.16624805295334966 2307 96-97 -0.3502795273784969 2307 98-99 -0.18252379763426774 2307 100-101 -0.3633041890317692 2201 1 0.07296143152035484 2201 2 0.07578422104453608 2201 3 0.051157091875523975 2201 4 -0.022123979406089234 2201 5 0.031109255257156576 2201 6 0.04868167807809698 2201 7 0.07972304674873953 2201 8 0.04316152393045769 2201 9 0.02908885963628194 2201 10-11 0.07440140875783641 2201 12-13 0.08316852049234313 2201 14-15 0.16016746717727415 2201 16-17 0.1465068744267768 2201 18-19 0.14685431367057333 2201 20-21 0.08675044994770786 2201 22-23 0.07676292673870222 2201 24-25 0.12238748630679908 2201 26-27 0.11481851289978096 2201 28-29 0.0879013401546942 2201 30-31 0.12961174856758362 2201 32-33 0.10599533016434037 2201 34-35 0.11884629872416497 2201 36-37 0.12738361925781305 2201 38-39 0.09436991752994572 2201 40-41 0.0947079060519016 2201 42-43 0.070157620496623 2201 44-45 0.045019149003671544 2201 46-47 0.026873182656565575 2201 48-49 0.06639683631635052 2201 50-51 -0.008107697009798187 2201 52-53 -0.007472749266490553 2201 54-55 0.02792711366088696 2201 56-57 0.009519021435828279 2201 58-59 -0.04893604791859474 2201 60-61 0.04178820487099699 2201 62-63 0.008380187667100358 2201 64-65 -0.02582359296627601 2201 66-67 -0.10912956511647565 2201 68-69 -0.11158856001549822 2201 70-71 -0.08656563033249398 2201 72-73 -0.20297992561096123 2201 74-75 -0.18151188262252305 2201 76-77 -0.04972081775558479 2201 78-79 -0.017954780074980192 2201 80-81 0.035501392875467275 2201 82-83 -0.03494542199710082 2201 84-85 0.019465451218664498 2201 86-87 0.03549204681124962 2201 88-89 0.030463746615517096 2201 90-91 0.008520634824641604 2201 92-93 -0.05755503297191922 2201 94-95 -0.060829255813260374 2201 96-97 -0.23649827286838132 2201 98-99 -0.12284857358005041 2201 100-101 -0.13632700745359827 1101 1 0.08721457432424273 1101 2 0.0951500651663082 1101 3 0.053532659651573056 1101 4 0.005988940722403413 1101 5 0.029789954680225605 1101 6 0.062013415250874004 1101 7 0.0669575478624651 1101 8 0.056055656368258155 1101 9 0.07122722892432876 1101 10-11 0.09032251662049617 1101 12-13 0.06457955409033644 1101 14-15 0.100144708147063 1101 16-17 0.07571013609562982 1101 18-19 0.12150771026090723 1101 20-21 0.07748455762999384 1101 22-23 0.051882121794449176 1101 24-25 0.07316269790846519 1101 26-27 0.09934073756837591 1101 28-29 0.10430820032200216 1101 30-31 0.0093119097561285 1101 32-33 0.04019813619304813 1101 34-35 0.06846146117496943 1101 36-37 0.037310081499612124 1101 38-39 0.011675158356894144 1101 40-41 -0.03469718292908652 1101 42-43 0.06758212129801677 1101 44-45 0.11177218134684352 1101 46-47 0.07648134587223865 1101 48-49 0.03102571266158094 1101 50-51 0.023796845604280747 1101 52-53 0.002399695788689371 1101 54-55 -0.04557783339058119 1101 56-57 -0.010364017403944104 1101 58-59 0.031097050498495094 1101 60-61 0.0670201396681307 1101 62-63 -0.01959666431825724 1101 64-65 -0.029649928535825154 1101 66-67 -0.12039009652470867 1101 68-69 -0.06596080844015972 1101 70-71 -0.23567697978051427 1101 72-73 -0.2892614863318812 1101 74-75 -0.2819947446437894 1101 76-77 -0.5020823183498173 1101 78-79 -0.014639804237098986 1101 80-81 0.15080980628589913 1101 82-83 0.04930661339619746 1101 84-85 -0.021483762698458975 1101 86-87 0.010305629304973252 1101 88-89 0.023646605390304387 1101 90-91 0.010611791679970395 1101 92-93 -0.002406128353584336 1101 94-95 -0.04431925133755499 1101 96-97 -0.12634371625637897 1101 98-99 -0.06661900091069839 1101 100-101 0.009452278963909322 2304 1 0.07818680363644148 2304 2 0.08133774317255416 2304 3 0.05291335448827539 2304 4 -0.00110344250744987 2304 5 -0.0064475916216224505 2304 6 0.029513479648869634 2304 7 0.05336840406162224 2304 8 0.06429287129918748 2304 9 0.03813250952538283 2304 10-11 0.05324038980761259 2304 12-13 0.03286111555938476 2304 14-15 0.090063275727708 2304 16-17 0.07228107824990104 2304 18-19 0.050532175423512626 2304 20-21 0.030422541611123677 2304 22-23 0.06417748475190166 2304 24-25 0.028034708754418602 2304 26-27 0.04361087889390802 2304 28-29 0.034772597558891505 2304 30-31 0.07699067118181802 2304 32-33 0.0972386910547911 2304 34-35 0.09430806058113461 2304 36-37 0.06618534074630844 2304 38-39 0.07501057916062592 2304 40-41 -0.06949656695072548 2304 42-43 -0.03289123621186718 2304 44-45 0.02281977912965516 2304 46-47 -0.0017419611371352062 2304 48-49 0.010685699620751166 2304 50-51 0.009851072953836137 2304 52-53 -0.055349589986875 2304 54-55 -0.02237172581283886 2304 56-57 -0.03143962540032419 2304 58-59 -0.03268204130989005 2304 60-61 -0.03523197852472748 2304 62-63 -0.08906355712846192 2304 64-65 -0.12203400748476412 2304 66-67 -0.1874140443754797 2304 68-69 -0.08449446019456985 2304 70-71 -0.13240209452711582 2304 72-73 -0.12795242960818598 2304 74-75 -0.20252531847056332 2304 76-77 -0.43329007513010964 2304 78-79 -0.1513877356189255 2304 80-81 0.07601698421054692 2304 82-83 0.08393196937288394 2304 84-85 0.025650615391704434 2304 86-87 0.05901413180281878 2304 88-89 0.04823016517374157 2304 90-91 0.015498320975289914 2304 92-93 -0.0545951968655487 2304 94-95 -0.10633749011911675 2304 96-97 -0.1173325847018063 2304 98-99 -0.05439429672563989 2304 100-101 0.1867806062284174 2202 1 0.08160846645331787 2202 2 0.07669220513860608 2202 3 0.05891581455017558 2202 4 -0.012491969148982207 2202 5 -5.417446293591865E-4 2202 6 0.030984604600064358 2202 7 0.02529424909149469 2202 8 0.04984718613987127 2202 9 0.02392829505367189 2202 10-11 0.027769237544220005 2202 12-13 0.023577482636369496 2202 14-15 0.04821017163256158 2202 16-17 0.03582401817235592 2202 18-19 -0.04378785247536143 2202 20-21 0.015795217014705543 2202 22-23 0.05047377289988475 2202 24-25 -0.0028674526652423538 2202 26-27 -0.020746363966495096 2202 28-29 -0.030283833638243607 2202 30-31 0.007899874264744255 2202 32-33 0.004336696693442832 2202 34-35 0.013628656609874668 2202 36-37 0.029678791622778533 2202 38-39 -0.01018155444322133 2202 40-41 -0.009945888675197523 2202 42-43 0.04578504867640021 2202 44-45 -0.005107701542037546 2202 46-47 0.06008122704667329 2202 48-49 0.03131562732477988 2202 50-51 -0.007905974862794096 2202 52-53 -0.031720825883368775 2202 54-55 0.008612934833486463 2202 56-57 -0.028114671016886916 2202 58-59 -0.05371105938055365 2202 60-61 0.020392631550564033 2202 62-63 0.042694056710480766 2202 64-65 -0.01688649965899458 2202 66-67 -0.09582552667716726 2202 68-69 -0.11282534406091571 2202 70-71 -0.06540566554871674 2202 72-73 -0.1752431777962542 2202 74-75 -0.18918036286437 2202 76-77 -0.3592602842979282 2202 78-79 -0.02737041551137054 2202 80-81 0.10344632251668173 2202 82-83 0.05020545393737308 2202 84-85 0.05821039998547661 2202 86-87 0.042806597984125005 2202 88-89 0.04863523723496854 2202 90-91 -0.006371567435422776 2202 92-93 0.06497648919768295 2202 94-95 0.014643420250266104 2202 96-97 -0.14800066374456122 2202 98-99 0.004578107364096695 2202 100-101 0.15336420196240752 1102 1 0.05858133226580975 1102 2 0.05250387305331827 1102 3 0.03302468339588671 1102 4 -0.029903195091229406 1102 5 -0.043276004540935276 1102 6 0.013368732508070025 1102 7 0.044106467123327775 1102 8 0.014997092539275059 1102 9 0.021331523290932353 1102 10-11 0.04806300020041476 1102 12-13 0.03789249178576881 1102 14-15 0.07871016058597036 1102 16-17 0.01179544352967099 1102 18-19 0.03717695078803018 1102 20-21 0.019839737376344146 1102 22-23 0.07079963497628938 1102 24-25 0.018026928003891385 1102 26-27 0.03775736768493232 1102 28-29 -0.009260091756964073 1102 30-31 -0.1332355676289083 1102 32-33 -0.07204886364104368 1102 34-35 0.012373004110479258 1102 36-37 -0.02152265225370087 1102 38-39 0.012208270722283032 1102 40-41 -0.007838797134326114 1102 42-43 -0.017398565542549704 1102 44-45 -0.03825873387277312 1102 46-47 -0.039465429055645984 1102 48-49 -0.03597268735855863 1102 50-51 -0.01999307354590485 1102 52-53 -0.059296095643709634 1102 54-55 -0.09587190704770876 1102 56-57 -0.05991075664173451 1102 58-59 -0.07774294163529305 1102 60-61 -0.007531322414280339 1102 62-63 -0.004459548218143539 1102 64-65 -0.1184867394891782 1102 66-67 -0.09292751200687377 1102 68-69 -0.10346683436986126 1102 70-71 -0.1790725109142315 1102 72-73 -0.13850409971012567 1102 74-75 -0.11790262908053961 1102 76-77 -0.4070128852585313 1102 78-79 -0.05718586046006635 1102 80-81 0.05065757383380998 1102 82-83 0.019958114307691233 1102 84-85 0.04733754609838314 1102 86-87 4.5752233571505485E-4 1102 88-89 -0.038419119051866346 1102 90-91 -0.10982040244990543 1102 92-93 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1213 40-41 -0.2144379844284643 1213 42-43 -0.229846168116687 1213 44-45 -0.22391499979322305 1213 46-47 -0.3915837822151573 1213 48-49 -0.24338998725173866 1213 50-51 -0.19500208772851124 1213 52-53 -0.162285810966722 1213 54-55 -0.0987547728321303 1213 56-57 -0.0943015326357255 1213 58-59 -0.10742491845687852 1213 60-61 -0.093332353574624 1213 62-63 -0.049663651337390036 1213 64-65 0.031014649533112504 1213 66-67 0.053656500908140004 1213 68-69 0.09665851462850128 1213 70-71 0.018738419340152745 1213 72-73 0.06254622757010964 1213 74-75 0.16283888836044014 1213 76-77 0.5458263045026612 1213 78-79 0.07595808677442761 1213 80-81 -0.10312563331049063 1213 82-83 -0.0920371768851993 1213 84-85 -0.05070163370312031 1213 86-87 -0.020106874561918175 1213 88-89 0.007711222130218687 1213 90-91 0.05646010959684844 1213 92-93 0.13199948918989435 1213 94-95 0.15340491998495054 1213 96-97 0.10421992760215204 1213 98-99 0.21509100357709343 1213 100-101 0.49759089102524356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 55.0 8 478.0 9 1372.0 10 939.0 11 316.0 12 267.0 13 387.0 14 912.0 15 2037.0 16 3615.0 17 5936.0 18 8421.0 19 11657.0 20 15872.0 21 21686.0 22 28149.0 23 36632.0 24 47619.0 25 61908.0 26 80006.0 27 104224.0 28 134456.0 29 176570.0 30 233117.0 31 312331.0 32 431431.0 33 629439.0 34 1025669.0 35 2019750.0 36 4438921.0 37 6071784.0 38 968421.0 39 45.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.39547336446754 20.883458585263938 31.826472542246748 27.89459550802177 2 28.422750498298434 21.42317611277563 31.11117972249841 19.042893666427528 3 20.698458128294263 19.50113740165147 40.05646769851609 19.743936771538184 4 20.7123480223469 19.80859048769458 38.83272043271786 20.64634105724065 5 29.96174803219973 18.968497061766723 32.147398672997824 18.92235623303572 6 30.543587082606543 19.707313303575482 31.446035557002055 18.303064056815916 7 29.78007074136922 27.125598227756015 24.162415954178236 18.931915076696534 8 27.008740919542984 21.147842302902628 31.733826086884378 20.10959069067001 9 22.85160988587108 20.395917467059324 35.72020168271205 21.032270964357544 10-11 28.555625566783526 21.54620842284951 28.078754779278697 21.819411231088264 12-13 27.14409070862618 26.1004700248767 24.010656718041844 22.744782548455277 14-15 26.445105678320974 23.76875891275309 25.82627575096172 23.959859657964216 16-17 25.948719677931564 23.298108036385948 25.255285720533543 25.497886565148942 18-19 26.102333791976097 24.085755568811646 25.73362199105321 24.078288648159056 20-21 24.940900303087904 24.539820345929424 26.065796906953626 24.45348244402904 22-23 25.087534829332863 24.46775078453039 25.754923456359347 24.689790929777402 24-25 25.67497999635662 24.459569765513802 25.198086737883706 24.667363500245877 26-27 25.377494321880935 24.61525209805655 25.504938656876387 24.502314923186127 28-29 25.075081072387288 25.337134430528184 25.105559146130364 24.48222535095416 30-31 24.98971001517534 25.202535139361103 25.024982343834978 24.782772501628575 32-33 24.88744506035346 25.45137239038531 25.103882052047283 24.557300497213948 34-35 24.575064087473343 25.579541432516333 25.157274504171184 24.688119975839136 36-37 24.77318959916273 25.44770281049838 25.05266634355378 24.726441246785104 38-39 24.755428702120426 24.95631070536108 25.27914016611489 25.0091204264036 40-41 24.900243270789773 25.14766858455338 24.793330951840858 25.158757192815994 42-43 24.841834405035605 25.168835701982516 24.86517470855733 25.124155184424545 44-45 25.025434490131627 25.151635785509058 24.675631913925567 25.14729781043375 46-47 24.672148472121133 25.08815081796984 25.16518916738698 25.07451154252205 48-49 24.834064593359916 24.85312599771992 24.962883892994796 25.34992551592537 50-51 24.796235496918708 25.23159683634108 25.396457408885333 24.575710257854876 52-53 25.136000412063638 24.77269447742557 25.47809561992417 24.613209490586623 54-55 24.551895935509467 25.12015307234547 24.9782243718171 25.349726620327967 56-57 24.673485414685068 25.326058176478362 25.294702296667047 24.705754112169526 58-59 24.814363717929723 25.48190970733291 25.104471449786324 24.59925512495104 60-61 25.13007969449264 25.464506198587312 24.881574319998997 24.52383978692105 62-63 24.79129628759347 25.651199970416094 25.219735450447228 24.337768291543206 64-65 25.04268896757772 25.428169811414687 24.887007287053585 24.64213393395401 66-67 25.595897107518056 25.24283600592024 25.084114238895346 24.077152647666363 68-69 25.01406803123853 24.875171971419473 25.724319107584442 24.386440889757555 70-71 25.08232714071236 25.12465940456211 25.324870120554642 24.468143334170893 72-73 24.63288789364452 24.846714947741276 25.790575677280025 24.72982148133418 74-75 24.655385882820116 24.894558256555843 25.755327503282 24.69472835734204 76-77 24.2862448791824 24.894868865636468 25.396576066632377 25.422310188548753 78-79 25.219078523514217 25.11222070838671 24.87673642838332 24.79196433971575 80-81 24.757047671796432 24.968819859730633 25.48806857547689 24.786063892996047 82-83 24.63575796694304 25.676736912418498 25.40217735251502 24.285327768123448 84-85 24.82384038844572 25.37811550254667 25.150996810558297 24.647047298449312 86-87 24.619956001502768 25.495412778245097 25.384293429046867 24.50033779120527 88-89 24.708795579036458 26.46128219322275 24.659907872885444 24.170014354855343 90-91 24.68881478672545 25.31344408816276 24.881984162500494 25.11575696261129 92-93 24.493014377325885 25.78788071902932 24.977465494269673 24.741639409375125 94-95 24.394974766002296 26.02518580422909 24.496127716388564 25.08371171338006 96-97 24.369981145010627 25.07535460506819 25.108585053038162 25.446079196883026 98-99 24.73110981062979 25.19442801717331 24.819692585817936 25.254769586378966 100-101 24.729655991260575 26.36367084554143 24.03186995341489 24.8748032097831 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2928.0 1 2200.0 2 1791.0 3 2026.0 4 2032.5 5 1842.5 6 1546.5 7 1478.5 8 1452.5 9 1475.0 10 1510.0 11 1526.0 12 1509.0 13 1563.0 14 1689.0 15 1822.0 16 1909.0 17 2056.5 18 2296.0 19 2502.0 20 2704.5 21 2968.0 22 3274.5 23 3728.0 24 4378.5 25 5105.5 26 5846.5 27 6968.5 28 8234.0 29 9307.5 30 11221.0 31 14184.0 32 19274.5 33 26689.0 34 36235.5 35 51060.0 36 70287.5 37 95197.0 38 127366.0 39 161066.0 40 204525.5 41 277726.5 42 370218.5 43 460054.5 44 583013.0 45 753025.0 46 912998.5 47 1090019.5 48 1243104.0 49 1255627.5 50 1185516.0 51 1143979.5 52 1087326.0 53 1042751.0 54 1010109.5 55 898369.0 56 749638.5 57 564961.0 58 386942.0 59 278571.5 60 209246.0 61 155392.5 62 116533.5 63 78746.5 64 44408.5 65 24258.5 66 13279.5 67 8010.5 68 6065.5 69 4670.5 70 3336.5 71 2461.0 72 1986.0 73 1603.5 74 1299.0 75 971.5 76 735.5 77 560.0 78 400.0 79 338.0 80 259.0 81 155.0 82 96.0 83 78.0 84 59.0 85 37.0 86 32.5 87 33.0 88 26.0 89 19.0 90 16.0 91 14.5 92 11.0 93 8.5 94 6.5 95 3.0 96 3.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.140573670476258 2 0.0 3 0.0 4 0.18222249456070388 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.800299340552384E-4 12-13 0.0 14-15 4.7705326391546594E-4 16-17 0.0 18-19 0.0 20-21 0.003606048585000758 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 5.0372083767471554E-5 32-33 0.0 34-35 1.688946338085811E-4 36-37 2.0445139882091395E-4 38-39 0.00939587515450896 40-41 0.007428400823826542 42-43 0.0010726290778720414 44-45 0.0011467056716477349 46-47 0.0021482212194951105 48-49 8.000272127774894E-5 50-51 9.896632928432648E-4 52-53 0.019233246807921052 54-55 0.017342812134765354 56-57 0.021257019349872997 58-59 0.04088435363668074 60-61 0.015156071086506883 62-63 0.0022815590882913584 64-65 0.007194318787495349 66-67 0.012646356089386388 68-69 0.02736093067699014 70-71 0.011570763947763319 72-73 0.018258398833832925 74-75 0.012302640694267172 76-77 0.022691142205370423 78-79 0.009111421034410296 80-81 0.00655133395352233 82-83 0.00183413646188617 84-85 0.019505848673015604 86-87 0.009345503070741487 88-89 0.012361901969287726 90-91 0.04516894382066685 92-93 0.034673772014526594 94-95 0.029174325692619116 96-97 0.02581124833520263 98-99 0.03234480390621879 100-101 0.24194897059016998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6874426E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.220441171148842 #Duplication Level Percentage of deduplicated Percentage of total 1 74.17494587826663 14.256751836263732 2 9.882812816330615 3.7990404468351677 3 3.8329170731060103 2.2101107135257823 4 2.4646646093274827 1.8948776452076568 5 1.2561347610539064 1.2071732138935858 6 0.9981258289312579 1.151065126582645 7 0.7383034656916039 0.9933362830146546 8 0.5360398046308447 0.8242337224241013 9 0.4243741253444121 0.7340992119666016 >10 4.379802847111705 17.52737491355357 >50 0.6505319288317485 8.797449975150592 >100 0.5510702353773388 21.021762598603587 >500 0.061055718209075946 8.159408851015048 >1k 0.04859437018444558 16.259503096004153 >5k 3.7998957533987227E-4 0.5384489400638686 >10k+ 2.4654802730598226E-4 0.6253634258952662 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.296578502877669E-5 0.0 0.0 2 8.296578502877669E-5 0.0 0.0 3 8.296578502877669E-5 0.0 0.0 4 8.296578502877669E-5 0.0 0.0 5 8.296578502877669E-5 0.0 0.0 6 8.889191253083216E-5 0.0 0.0 7 8.889191253083216E-5 0.0 0.0 8 1.0074416753494311E-4 0.0 0.0 9 1.0074416753494311E-4 0.0 0.0 10-11 1.3630093254727598E-4 0.0 0.0 12-13 1.8667301631474753E-4 0.0 0.0 14-15 2.3111897258016361E-4 0.0 0.0 16-17 3.022325026048293E-4 0.0 0.0 18-19 4.0890279764182796E-4 0.0 0.0 20-21 5.422406664380762E-4 0.0 0.0 22-23 7.140983639976851E-4 0.0 0.0 24-25 8.94845252810377E-4 0.0 0.0 26-27 0.0011763363091580122 0.0 0.0 28-29 0.001404492217987148 0.0 0.0 30-31 0.0016859832743347832 0.0 0.0 32-33 0.0019793265856865292 0.0 0.0 34-35 0.0023882293833283573 0.0 0.0 36-37 0.0028712087747458787 0.0 0.0 38-39 0.0034815999074575927 0.0 0.0 40-41 0.004171993761447056 0.0 0.0 42-43 0.0051112849705228485 0.0 0.0 44-45 0.006287621279680861 0.0 0.0 46-47 0.007603221585137177 0.0 0.0 48-49 0.009271426476965794 0.0 0.0 50-51 0.011277420636411573 0.0 0.0 52-53 0.014056774434875591 0.0 0.0 54-55 0.01715021299094855 0.0 0.0 56-57 0.021325169816146634 0.0 0.0 58-59 0.026394971894155097 0.0 0.0 60-61 0.03263222109006848 0.0 0.0 62-63 0.041065100525493434 0.0 0.0 64-65 0.051672868754172735 0.0 0.0 66-67 0.06470146006744171 0.0 0.0 68-69 0.08102497827185351 0.0 0.0 70-71 0.10087454233998833 0.0 0.0 72-73 0.12627688787754915 0.0 0.0 74-75 0.15715497522700922 0.0 0.0 76-77 0.1987712056101938 0.0 0.0 78-79 0.25280859923768667 0.0 0.0 80-81 0.3174863547951201 0.0 0.0 82-83 0.4015099535830138 0.0 0.0 84-85 0.49428347962769226 0.0 0.0 86-87 0.6042131447908213 0.0 0.0 88-89 0.734706472386083 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 181415 0.0 73.86349 1 TTTGTGT 193430 0.0 69.35808 2 TTGTGTT 220875 0.0 62.48853 3 TAACTTT 16705 0.0 61.47369 1 TGTGTTT 235125 0.0 58.99467 4 AACTTTG 17845 0.0 58.025814 2 GTGTTTG 254630 0.0 54.46395 5 ACTTTGT 39940 0.0 51.804653 1 TTTGAGG 87155 0.0 37.492058 8 TTGAGTC 25020 0.0 37.241444 4 GTTTGAG 222710 0.0 36.149357 7 GTTTGAA 23055 0.0 35.89744 2 TGTTTGA 389050 0.0 35.631557 6 TTGAGGG 42420 0.0 34.899857 9 TTTGAGC 52020 0.0 34.081673 8 TTGAGGT 23345 0.0 33.49793 9 TTTGAGT 86360 0.0 32.847656 3 TTTGAGA 25725 0.0 31.1739 8 TTGAGCC 26105 0.0 31.102163 9 TTGATTC 10375 0.0 30.945324 4 >>END_MODULE