FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30348.34100000035cf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30348.34100000035cf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16874426
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1814150.073.863491
TTTGTGT1934300.069.358082
TTGTGTT2208750.062.488533
TAACTTT167050.061.473691
TGTGTTT2351250.058.994674
AACTTTG178450.058.0258142
GTGTTTG2546300.054.463955
ACTTTGT399400.051.8046531
TTTGAGG871550.037.4920588
TTGAGTC250200.037.2414444
GTTTGAG2227100.036.1493577
GTTTGAA230550.035.897442
TGTTTGA3890500.035.6315576
TTGAGGG424200.034.8998579
TTTGAGC520200.034.0816738
TTGAGGT233450.033.497939
TTTGAGT863600.032.8476563
TTTGAGA257250.031.17398
TTGAGCC261050.031.1021639
TTGATTC103750.030.9453244
Produced by FastQC (version 0.11.2)