FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30304.34100000035cbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30304.34100000035cbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19625347
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1959450.063.9831121
TAACTTT220500.061.418391
TTTGTGT2069950.060.6368032
AACTTTG227500.060.2026672
ACTTTGT617550.059.2097171
TTGTGTT2374000.054.5680433
TGTGTTT2498350.052.128144
GTGTTTG3050400.042.804935
TTTGAGG857150.035.608428
GTTTGAG2061700.034.7925647
TTGAGGG409200.034.115269
TGTTTGA3870650.033.6394546
TTGAGGT244450.031.7394269
TTTGAGA259750.030.4728988
GTTTGAC763450.030.0773377
TTTGAGC476900.029.8595228
TTGAGTC225000.029.4103454
TTGAGGC152250.029.3473829
GTTTGAA265400.028.7334732
TTTGACA100100.026.7059528
Produced by FastQC (version 0.11.2)