##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30301.34100000035c7c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14269267 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74990109863387 33.0 33.0 33.0 33.0 33.0 2 32.766341816997326 33.0 33.0 33.0 33.0 33.0 3 32.80047047966794 33.0 33.0 33.0 33.0 33.0 4 36.66899343883607 37.0 37.0 37.0 37.0 37.0 5 36.710729429899935 37.0 37.0 37.0 37.0 37.0 6 36.68588288382298 37.0 37.0 37.0 37.0 37.0 7 36.65829351991241 37.0 37.0 37.0 37.0 37.0 8 36.65407781633072 37.0 37.0 37.0 37.0 37.0 9 36.64563603722602 37.0 37.0 37.0 37.0 37.0 10-11 36.61947400661856 37.0 37.0 37.0 37.0 37.0 12-13 36.60442908525014 37.0 37.0 37.0 37.0 37.0 14-15 38.75524338426073 40.0 37.0 40.0 37.0 40.0 16-17 38.71532773897916 40.0 37.0 40.0 37.0 40.0 18-19 38.68365382748813 40.0 37.0 40.0 37.0 40.0 20-21 38.64611517886658 40.0 37.0 40.0 37.0 40.0 22-23 38.593374487981755 40.0 37.0 40.0 37.0 40.0 24-25 38.54393662968112 40.0 37.0 40.0 37.0 40.0 26-27 38.47212666915547 40.0 37.0 40.0 37.0 40.0 28-29 38.40126956065788 40.0 37.0 40.0 37.0 40.0 30-31 38.36712712713274 40.0 37.0 40.0 37.0 40.0 32-33 38.30759649391942 40.0 37.0 40.0 37.0 40.0 34-35 38.23805455458925 40.0 37.0 40.0 37.0 40.0 36-37 38.14830901965742 40.0 37.0 40.0 37.0 40.0 38-39 38.045377628717716 40.0 37.0 40.0 37.0 40.0 40-41 37.95727072035305 40.0 37.0 40.0 35.0 40.0 42-43 37.827829733650645 40.0 37.0 40.0 33.0 40.0 44-45 37.72533301815714 40.0 37.0 40.0 33.0 40.0 46-47 37.56088893704211 40.0 37.0 40.0 33.0 40.0 48-49 37.38455805052915 38.5 37.0 40.0 33.0 40.0 50-51 37.21296745656241 37.0 37.0 40.0 33.0 40.0 52-53 36.9876448103466 37.0 37.0 40.0 33.0 40.0 54-55 36.747126043685356 37.0 37.0 40.0 33.0 40.0 56-57 36.50207649068449 37.0 37.0 40.0 33.0 40.0 58-59 36.241462508200314 37.0 37.0 40.0 33.0 40.0 60-61 35.96739156257991 37.0 37.0 38.5 33.0 40.0 62-63 35.726425716191315 37.0 35.0 37.0 33.0 40.0 64-65 35.4438750427755 37.0 33.0 37.0 33.0 40.0 66-67 35.16992666827245 37.0 33.0 37.0 33.0 40.0 68-69 34.8884364557759 37.0 33.0 37.0 30.0 38.5 70-71 34.6506587198908 37.0 33.0 37.0 27.0 37.0 72-73 34.46784333771314 37.0 33.0 37.0 27.0 37.0 74-75 34.2036501594651 37.0 33.0 37.0 27.0 37.0 76-77 31.310118978080652 33.0 30.0 35.0 24.5 37.0 78-79 33.943247049760856 35.0 33.0 37.0 27.0 37.0 80-81 34.41083970886521 37.0 33.0 37.0 33.0 37.0 82-83 34.37742453764443 37.0 33.0 37.0 33.0 37.0 84-85 34.30489845764327 37.0 33.0 37.0 30.0 37.0 86-87 34.163575010545394 37.0 33.0 37.0 27.0 37.0 88-89 34.05597137540421 37.0 33.0 37.0 27.0 37.0 90-91 33.97087912784868 37.0 33.0 37.0 27.0 37.0 92-93 33.88295772305614 37.0 33.0 37.0 27.0 37.0 94-95 33.75549693617759 37.0 33.0 37.0 27.0 37.0 96-97 33.66062135497219 37.0 33.0 37.0 27.0 37.0 98-99 33.507188175818705 37.0 33.0 37.0 27.0 37.0 100-101 31.623379287807847 35.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10624162224046785 2306 2 0.09390858928267676 2306 3 0.08016901425310152 2306 4 0.03536109696079848 2306 5 0.04936577474168047 2306 6 0.08626810358086345 2306 7 0.11486034600676476 2306 8 0.12145866335094979 2306 9 0.10781430136315606 2306 10-11 0.14088965590448055 2306 12-13 0.10467242908949004 2306 14-15 0.18518969149867814 2306 16-17 0.22850900369042648 2306 18-19 0.17774841432938615 2306 20-21 0.15661496199600577 2306 22-23 0.15628274989585833 2306 24-25 0.15234226162824882 2306 26-27 0.2576260107931674 2306 28-29 0.20206676830646586 2306 30-31 0.19471284095535424 2306 32-33 0.2009386833614215 2306 34-35 0.16595875873120747 2306 36-37 0.1677890957491286 2306 38-39 0.20049628070920988 2306 40-41 0.14441548528061077 2306 42-43 0.1566334472464277 2306 44-45 0.18979343811189153 2306 46-47 0.17362842098468434 2306 48-49 0.11756776327563756 2306 50-51 0.06420238908962261 2306 52-53 0.020320050432744097 2306 54-55 -0.02943069562488887 2306 56-57 -3.7218587457488184E-4 2306 58-59 0.01551360471395924 2306 60-61 -0.021552125769467523 2306 62-63 0.019471451452147903 2306 64-65 0.008390610603186133 2306 66-67 -0.17192230649109774 2306 68-69 -0.21815518380405052 2306 70-71 -0.17838653993369036 2306 72-73 -0.131413640318911 2306 74-75 -0.14035026668511108 2306 76-77 -0.4136356845305009 2306 78-79 0.0010705873350076445 2306 80-81 0.09985908315174186 2306 82-83 0.03603762987098236 2306 84-85 -0.05354994164926552 2306 86-87 -0.08732460478047699 2306 88-89 -0.020606589430279598 2306 90-91 -0.1207406543524101 2306 92-93 -0.159512986318866 2306 94-95 -0.09929073823983714 2306 96-97 -0.24002862944730907 2306 98-99 -0.10188303055561221 2306 100-101 -0.3822677889492887 2307 1 0.09750881241895115 2307 2 0.09794252875006038 2307 3 0.06414680230641778 2307 4 0.008479832024200107 2307 5 0.026452897344483972 2307 6 0.05616292133523615 2307 7 0.05426067378027 2307 8 0.08452211051087488 2307 9 0.07229512264041915 2307 10-11 0.10925712413615685 2307 12-13 0.10339687038062806 2307 14-15 0.12533560745583117 2307 16-17 0.10114651272548514 2307 18-19 0.11773916010268692 2307 20-21 0.09255380305247485 2307 22-23 0.1468507166202997 2307 24-25 0.07371103406942581 2307 26-27 0.11549731540721808 2307 28-29 0.1212846159437504 2307 30-31 0.1192818370287867 2307 32-33 0.10915846181670474 2307 34-35 0.14617433063156682 2307 36-37 0.1228666472158011 2307 38-39 0.08037438324192436 2307 40-41 0.10019625536774868 2307 42-43 0.13478969718251932 2307 44-45 0.09423833894319955 2307 46-47 0.07535297746835568 2307 48-49 -0.016736238348912025 2307 50-51 0.1005691410685472 2307 52-53 -0.007137107777126062 2307 54-55 0.05306150929411757 2307 56-57 0.02439891188864607 2307 58-59 0.03724873998231715 2307 60-61 -0.015627215970965835 2307 62-63 -0.020040423794391415 2307 64-65 -0.0943764225938537 2307 66-67 -0.15914621492583336 2307 68-69 -0.14396172817436792 2307 70-71 -0.21156102394798637 2307 72-73 -0.27887996470582976 2307 74-75 -0.32660555785405165 2307 76-77 -0.5834817525882379 2307 78-79 -0.1386755329250775 2307 80-81 -0.08826446396395937 2307 82-83 -0.1327096114276074 2307 84-85 -0.14125305686338407 2307 86-87 -0.08949179623810721 2307 88-89 -0.1719412055384666 2307 90-91 -0.2040854849831888 2307 92-93 -0.17565941240938088 2307 94-95 -0.1643700319803898 2307 96-97 -0.3856477050098306 2307 98-99 -0.23694704109466613 2307 100-101 -0.37866921862609004 2201 1 0.0817997608985408 2201 2 0.08162770415151499 2201 3 0.06259841880196149 2201 4 -0.020135188623065403 2201 5 -0.005797344479162803 2201 6 0.04772748468655408 2201 7 0.06720259272694307 2201 8 0.04834108528223169 2201 9 0.06126552044840139 2201 10-11 0.06469895729532027 2201 12-13 0.10314856411730489 2201 14-15 0.16660271477891797 2201 16-17 0.1507835668408859 2201 18-19 0.16636065204125572 2201 20-21 0.15164439724586032 2201 22-23 0.11867033107507297 2201 24-25 0.13762752333400385 2201 26-27 0.15534620962248624 2201 28-29 0.1460841564464559 2201 30-31 0.13087053094803736 2201 32-33 0.11480288297442343 2201 34-35 0.16215808021234324 2201 36-37 0.1683636368837469 2201 38-39 0.11343612628860456 2201 40-41 0.1425859569178769 2201 42-43 0.14768625396348511 2201 44-45 0.13311810144453773 2201 46-47 0.14637751981629776 2201 48-49 0.17043992191897672 2201 50-51 0.1135241142834289 2201 52-53 0.037759846863622215 2201 54-55 0.0430615432853898 2201 56-57 0.11772444179644737 2201 58-59 0.0042458018412645515 2201 60-61 0.0018201493491289966 2201 62-63 0.013153577684391848 2201 64-65 0.03343499575522202 2201 66-67 -0.05610455424810823 2201 68-69 -0.10511768079117445 2201 70-71 -0.12831612458158048 2201 72-73 -0.19798620945336864 2201 74-75 -0.15352410634244507 2201 76-77 -0.05407185406999915 2201 78-79 0.024595761475616484 2201 80-81 0.04001735316427357 2201 82-83 0.017142380849584526 2201 84-85 0.0529206039127601 2201 86-87 0.02201544001873401 2201 88-89 0.06815677997671798 2201 90-91 -0.022397742107429508 2201 92-93 -0.01954559602442174 2201 94-95 -0.005314974855622268 2201 96-97 -0.10269877253008985 2201 98-99 -0.1217011692540737 2201 100-101 -0.10007778144909096 1101 1 0.05534687093233259 1101 2 0.0503843703507556 1101 3 0.035527655256942126 1101 4 -0.046139603111477356 1101 5 -0.02356698066630969 1101 6 0.017521854635496936 1101 7 0.03756869264905305 1101 8 0.024916099107855416 1101 9 0.02563413539051851 1101 10-11 0.051895540892878955 1101 12-13 0.0575255025129735 1101 14-15 0.07791217788314952 1101 16-17 0.00928185920118807 1101 18-19 0.07939504067527281 1101 20-21 0.04349178354940619 1101 22-23 0.021829464519257158 1101 24-25 0.0309359834351568 1101 26-27 0.0142753729275924 1101 28-29 0.009995573319969253 1101 30-31 -0.015661436759181413 1101 32-33 -0.00480720469614937 1101 34-35 -0.001470248941430441 1101 36-37 0.005407349394438654 1101 38-39 0.002683679713811671 1101 40-41 -0.09479812254412678 1101 42-43 0.050290362770425645 1101 44-45 0.03479127192380105 1101 46-47 0.014870869248667873 1101 48-49 -0.031374589436858 1101 50-51 -0.017499401882091092 1101 52-53 -0.11139836052142016 1101 54-55 -0.10782860488407664 1101 56-57 -0.04325456828852481 1101 58-59 0.005440785399230208 1101 60-61 0.06845549208813395 1101 62-63 -0.0563645680362157 1101 64-65 -0.0874396930543071 1101 66-67 -0.15075994323847652 1101 68-69 -0.08848240634561222 1101 70-71 -0.19505736389820783 1101 72-73 -0.29488716286024186 1101 74-75 -0.2592064777701637 1101 76-77 -0.4854097742947552 1101 78-79 -0.04880123956183979 1101 80-81 0.013640925300066442 1101 82-83 -0.06323581915369658 1101 84-85 -0.03234059651606458 1101 86-87 -0.0035170553691799 1101 88-89 0.033941823772543955 1101 90-91 0.002289204525943944 1101 92-93 -0.09926606184036046 1101 94-95 -0.12377625292650407 1101 96-97 -0.10050303577128261 1101 98-99 -0.06029948343792313 1101 100-101 -0.038348738583266595 2304 1 0.0660209346986278 2304 2 0.0749446928001376 2304 3 0.030846226500408136 2304 4 0.02287733806257819 2304 5 0.002603525249270433 2304 6 0.04229505879678186 2304 7 0.06726484461839988 2304 8 0.06942638638052756 2304 9 0.061386230790944296 2304 10-11 0.06285906196796276 2304 12-13 0.024148951355208226 2304 14-15 0.04868471711900213 2304 16-17 0.05089252409648992 2304 18-19 0.019804547715942533 2304 20-21 0.0247801296122816 2304 22-23 0.05640396753279475 2304 24-25 -0.017557155815495662 2304 26-27 -0.008460970461122486 2304 28-29 0.008560968219889276 2304 30-31 0.042471441724565295 2304 32-33 0.028193497257412048 2304 34-35 0.0031433090379238138 2304 36-37 0.05897111140848921 2304 38-39 0.061472856439486634 2304 40-41 -0.09291653359633045 2304 42-43 -0.06957095050339035 2304 44-45 0.03415389704959182 2304 46-47 0.06736555096372854 2304 48-49 -0.00785817846641379 2304 50-51 -0.015912774709548216 2304 52-53 -0.032995951179756844 2304 54-55 -0.011728968903618409 2304 56-57 -0.01949715695668175 2304 58-59 -0.05732721728118406 2304 60-61 -0.02924466278567195 2304 62-63 -0.09771215119118182 2304 64-65 -0.11179410611597262 2304 66-67 -0.18353670867665528 2304 68-69 -0.14149552411726773 2304 70-71 -0.16807277209247218 2304 72-73 -0.17138122237299314 2304 74-75 -0.23679189867416994 2304 76-77 -0.3859408755641134 2304 78-79 -0.10745473118922177 2304 80-81 0.07366939030754338 2304 82-83 0.059047836913208585 2304 84-85 -0.00930020545393262 2304 86-87 0.030828490067591474 2304 88-89 0.06669746758493034 2304 90-91 0.04532482759100276 2304 92-93 -0.11894647364672295 2304 94-95 -0.13413910262746498 2304 96-97 -0.19286109888700764 2304 98-99 -0.0677633917628171 2304 100-101 0.0018608420012995452 2202 1 0.06372131564966566 2202 2 0.05615274831865236 2202 3 0.043474175498310785 2202 4 -0.02583397977572588 2202 5 -0.016270471682211962 2202 6 0.020646410623228917 2202 7 0.019411437525057806 2202 8 0.04909689065152634 2202 9 0.014330936179540288 2202 10-11 0.011025479725098819 2202 12-13 0.036838957573948505 2202 14-15 0.010572330673312536 2202 16-17 0.017518138890601165 2202 18-19 -0.03392821631268106 2202 20-21 0.01054778789040256 2202 22-23 0.04713526635600118 2202 24-25 0.016323275397262194 2202 26-27 0.010633092364464858 2202 28-29 -0.036126001486067594 2202 30-31 0.016865602557352588 2202 32-33 0.01384748754065157 2202 34-35 0.03768237935071994 2202 36-37 0.03783751547970127 2202 38-39 0.09455900257480465 2202 40-41 0.04603734718566699 2202 42-43 0.06676332837641041 2202 44-45 0.03313035241571072 2202 46-47 0.0785817697047122 2202 48-49 0.05511413730526016 2202 50-51 -0.004844206975015197 2202 52-53 -0.03451415825145432 2202 54-55 0.018333557272917744 2202 56-57 -0.0180596903638488 2202 58-59 -0.010927158536446768 2202 60-61 0.09073619190571236 2202 62-63 0.0028888973085159364 2202 64-65 0.004346905014450897 2202 66-67 -0.05811223916034436 2202 68-69 -0.030528867932552828 2202 70-71 -0.04186384248552599 2202 72-73 -0.16758111279479948 2202 74-75 -0.15347962099778556 2202 76-77 -0.3786943756614072 2202 78-79 -0.04516082286451706 2202 80-81 0.12278687665134669 2202 82-83 0.03422500776327553 2202 84-85 0.06914147311862706 2202 86-87 0.0854761813308329 2202 88-89 0.10527889850587258 2202 90-91 0.04038167549730787 2202 92-93 0.08890502842508141 2202 94-95 0.036618911479123994 2202 96-97 -0.07683160530253019 2202 98-99 0.07273518212456764 2202 100-101 0.13160908500068658 1102 1 0.033136348759377654 1102 2 0.06314298968010945 1102 3 0.03330197011371183 1102 4 -0.04927558939581189 1102 5 -0.05001534569757382 1102 6 0.03697133643126449 1102 7 0.024127381866783537 1102 8 0.03426272903037386 1102 9 0.03128717657612867 1102 10-11 0.05883976936346613 1102 12-13 0.04934829649024408 1102 14-15 0.055033138258423264 1102 16-17 -0.0014407267898164378 1102 18-19 0.029592187728660235 1102 20-21 0.011152813734163658 1102 22-23 0.01636121285981318 1102 24-25 -0.02119954772155097 1102 26-27 0.010849301260726918 1102 28-29 -0.004904398139366606 1102 30-31 -0.05581424900485388 1102 32-33 -0.013501142507969632 1102 34-35 0.0740624756162589 1102 36-37 0.001022629022706667 1102 38-39 0.03297563019305727 1102 40-41 6.137303519508919E-4 1102 42-43 -0.03183671260838139 1102 44-45 -0.024365825457273615 1102 46-47 -0.02846439615238694 1102 48-49 -0.03159114432157395 1102 50-51 -0.030140776075811004 1102 52-53 -0.04229774263588837 1102 54-55 -0.0828130295375118 1102 56-57 -0.029938484118268605 1102 58-59 -0.06868135082181936 1102 60-61 -0.07203172566919847 1102 62-63 -0.07113770741417369 1102 64-65 -0.13481743862484308 1102 66-67 -0.18439945793168278 1102 68-69 -0.14494357660068857 1102 70-71 -0.19184401692481146 1102 72-73 -0.1797113167159523 1102 74-75 -0.20933114934847197 1102 76-77 -0.46734553735462825 1102 78-79 -0.10280530772951124 1102 80-81 0.02296152368327853 1102 82-83 -0.009604036401050564 1102 84-85 -0.06685706445772865 1102 86-87 -0.05127697145908883 1102 88-89 -0.08205758229552629 1102 90-91 -0.18938124875494822 1102 92-93 -0.22424605081125293 1102 94-95 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1213 38-39 -0.27612405438338783 1213 40-41 -0.27625150293010137 1213 42-43 -0.25158144265524385 1213 44-45 -0.25306274996498246 1213 46-47 -0.4207370588069921 1213 48-49 -0.23238330271524177 1213 50-51 -0.21435876799215947 1213 52-53 -0.17628089836342298 1213 54-55 -0.11488386413922314 1213 56-57 -0.09594185947473477 1213 58-59 -0.14329204787713934 1213 60-61 -0.1098072398949057 1213 62-63 -0.05284158402299255 1213 64-65 -0.014089873262427943 1213 66-67 0.04132078165992681 1213 68-69 0.0657474215541285 1213 70-71 -0.011097467010742434 1213 72-73 0.014805925451888413 1213 74-75 0.10145621346330813 1213 76-77 0.5153009170866412 1213 78-79 0.08386218422264591 1213 80-81 -0.09742255708049186 1213 82-83 -0.11345470372996402 1213 84-85 -0.07425824387115654 1213 86-87 -0.10745800157121721 1213 88-89 -0.04511903727161126 1213 90-91 -0.016937870967247193 1213 92-93 0.03336842437688148 1213 94-95 0.10505557661847575 1213 96-97 0.03669702338217462 1213 98-99 0.13474983283528275 1213 100-101 0.45927712093465445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 25.0 8 422.0 9 1316.0 10 825.0 11 281.0 12 228.0 13 295.0 14 686.0 15 1416.0 16 2650.0 17 4343.0 18 6398.0 19 8960.0 20 12161.0 21 16176.0 22 21457.0 23 28019.0 24 36453.0 25 46987.0 26 61760.0 27 79526.0 28 103741.0 29 135839.0 30 178316.0 31 239909.0 32 332653.0 33 482646.0 34 782191.0 35 1528654.0 36 3499341.0 37 5411714.0 38 1243815.0 39 63.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.998693267859174 24.183281885141472 30.741049060282737 25.07697578671661 2 25.95544676541549 24.169657768685664 29.914935364234196 19.95996010166465 3 21.024275458578217 22.10041342698262 36.22346543799342 20.65184567644575 4 20.897403898102134 22.511731131282303 35.30860765065995 21.282257319955615 5 26.853194351188463 21.73891623164666 31.456275925035253 19.951613492129624 6 27.969250277537032 22.260765041399814 30.325979603577398 19.44400507748576 7 26.805672638965966 27.05756364359851 26.242020700853097 19.89474301658242 8 25.552272586952085 23.59750504353167 30.258877348079615 20.59134502143663 9 22.27534883186361 22.669475593946068 33.74455744643365 21.310618127756666 10-11 25.968735840301076 23.61553338441556 28.857860178852274 21.55787059643109 12-13 25.18837512816881 26.68484653065921 25.873087944881824 22.253690396290153 14-15 24.970752547542723 24.986576849851655 27.14949547071395 22.89317513189167 16-17 24.478671539329948 24.870215828185145 26.826059110114066 23.82505352237084 18-19 24.36597128640175 25.323641361535948 27.150231332835805 23.1601560192265 20-21 23.611455931188193 25.712079789113435 27.53170424229206 23.144760037406314 22-23 23.733468579710507 25.878557742314307 26.984196174898123 23.403777503077066 24-25 24.126838470399356 25.78377712043653 26.66909589679694 23.420288512367176 26-27 24.02948588739702 25.783219978994016 26.88707135412071 23.30022277948825 28-29 23.860983889361663 26.264506088504756 26.475820376757962 23.39868964537562 30-31 23.867670105585344 26.16556742158188 26.428611850121897 23.53815062271088 32-33 23.71966618887992 26.478984519667335 26.39730197773999 23.404047313712752 34-35 23.552139178250616 26.46562280362354 26.535100920924947 23.447137097200894 36-37 23.745201912815467 26.389325337011925 26.451963624100756 23.41350912607185 38-39 23.889778613846673 26.09193198965785 26.52453106043456 23.493758336060917 40-41 23.645778330182488 26.345751739437045 26.280459334452004 23.72801059592846 42-43 23.649336749995612 26.210617543520552 26.275726804330883 23.864318902152952 44-45 23.826441648083975 26.081180435798945 26.26775838677456 23.824619529342524 46-47 23.484913470181485 26.141180355616818 26.4748523262462 23.8990538479555 48-49 23.494795574217132 25.89768298606034 26.40638177922392 24.20113966049861 50-51 23.653184268213494 25.998673848660715 26.66306354555177 23.68507833757402 52-53 23.604154162793204 26.067331222898538 26.741326875274357 23.5871877390339 54-55 23.597792550726105 26.11408005043916 26.29419187383481 23.993935524999927 56-57 23.72640629596765 26.30531214910375 26.484109549691308 23.484172005237294 58-59 23.579486219518778 26.644939561629887 26.44417592294625 23.331398295905082 60-61 23.998512796395918 26.409411563831597 26.149136914796895 23.44293872497559 62-63 23.720006531669487 26.58096887365144 26.500980451245855 23.19804414343322 64-65 23.87684302368407 26.42204197208659 26.325943635345094 23.37517136888424 66-67 24.062565873685482 26.28693635561583 26.507229349503103 23.14326842119558 68-69 23.84281230643438 26.073371763484644 26.861633369290335 23.222182560790646 70-71 23.73308638579012 26.381998295080706 26.638374001119676 23.246541318009502 72-73 23.525256420078573 26.088640609269348 26.884888835274673 23.50121413537741 74-75 23.570705271563117 26.092267029544004 26.94609880288678 23.3909288960061 76-77 23.55040979706682 26.13319066214002 26.692583942726372 23.623815598066788 78-79 23.704011231646255 26.12413270783131 26.39856343255011 23.773292627972324 80-81 23.607258404346002 26.029998344230165 26.703130981906554 23.65961226951728 82-83 23.333232298367353 26.64766158075336 26.7189175125425 23.300188608336786 84-85 23.49672996223491 26.346250186365133 26.4304202848255 23.72659956657446 86-87 23.4266843534734 26.428040090453486 26.653013861093854 23.49226169497926 88-89 23.49825232924933 27.274622245082387 26.04061484563588 23.1865105800324 90-91 23.508647669125978 26.491501321936344 26.198364036067616 23.80148697287006 92-93 23.4726028972242 26.66717773863065 26.19704060589439 23.663178758250762 94-95 23.395457108468253 26.94393150447114 25.94960900602135 23.711002381039258 96-97 23.331503973897245 26.4445250898551 26.14253707922776 24.081433857019892 98-99 23.533580490365562 26.422581600500628 26.02970013524152 24.01413777389229 100-101 23.619084772596793 27.057053620379527 25.537333455853734 23.786528151169943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 32931.0 1 18936.0 2 9039.5 3 11435.5 4 10511.0 5 7882.5 6 4127.0 7 4190.5 8 5013.0 9 5243.5 10 4472.5 11 3302.5 12 2376.0 13 1893.5 14 1746.0 15 1738.0 16 1819.0 17 2014.0 18 2235.5 19 2508.5 20 2946.0 21 3526.0 22 4355.5 23 5528.5 24 7089.5 25 9207.0 26 11823.5 27 14839.0 28 18499.5 29 23131.5 30 29880.0 31 40235.5 32 54795.0 33 73015.5 34 96706.5 35 130180.5 36 170505.5 37 216356.5 38 265722.0 39 318474.0 40 377748.0 41 438143.0 42 497732.0 43 576978.5 44 665441.5 45 743567.0 46 820363.0 47 897736.5 48 933341.0 49 903624.0 50 842192.0 51 770559.0 52 690237.5 53 623755.0 54 565277.0 55 508410.0 56 468540.0 57 387667.0 58 275792.5 59 196679.5 60 147577.0 61 110816.0 62 77114.5 63 48061.5 64 28544.0 65 16951.5 66 10448.0 67 6917.0 68 5541.0 69 4518.0 70 3590.5 71 2819.0 72 2179.0 73 1693.0 74 1270.5 75 890.5 76 657.5 77 495.0 78 403.5 79 338.0 80 244.0 81 177.5 82 121.5 83 79.5 84 52.0 85 37.0 86 32.5 87 28.5 88 24.5 89 22.0 90 21.5 91 18.0 92 9.5 93 6.5 94 5.0 95 3.5 96 3.0 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14002821588523084 2 0.0 3 0.0 4 0.18348524840133695 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.164399755082024E-4 12-13 0.0 14-15 5.291091686769895E-4 16-17 0.0 18-19 0.0 20-21 0.003878965892221373 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 3.1536308066840434E-5 32-33 0.0 34-35 1.8220977994174472E-4 36-37 2.733146699126171E-4 38-39 0.010070594376011046 40-41 0.008024238385896066 42-43 0.001229916014606777 44-45 0.0011493232273248513 46-47 0.0021935254277602348 48-49 1.226411980377128E-4 50-51 0.0010967627138801174 52-53 0.02000453141706578 54-55 0.018042272248462377 56-57 0.02186517359300937 58-59 0.04188722518122339 60-61 0.015575432150789525 62-63 0.002291638386190405 64-65 0.007498633251448725 66-67 0.013346866380732802 68-69 0.02858591124547603 70-71 0.012120454400355673 72-73 0.019174075304638983 74-75 0.012751180561692483 76-77 0.0235786463313077 78-79 0.009583533618089843 80-81 0.006864403055882267 82-83 0.001958755134373756 84-85 0.020449543764231196 86-87 0.009681646576520013 88-89 0.01295441454701212 90-91 0.04671928838390928 92-93 0.035958399264657395 94-95 0.029977012834646658 96-97 0.026630660145331924 98-99 0.03354411968042928 100-101 0.2446026134348737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4269267E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.493660250273024 #Duplication Level Percentage of deduplicated Percentage of total 1 80.70996213871487 27.032720506865154 2 9.209590752087731 6.169258073889659 3 3.494328315193313 3.5111353617598136 4 1.43874822709612 1.9275577721616044 5 0.8688022928608388 1.4549684410869572 6 0.49458681802990984 0.9939313708414446 7 0.37862245079801005 0.8877016211107985 8 0.37999705248784305 1.0181993737706385 9 0.2592995731502783 0.7816402625522615 >10 2.0716737796229188 14.185942599829145 >50 0.32991957377351605 7.736121974256896 >100 0.32379655420041165 21.28729128274955 >500 0.029430295139318864 6.77170342612831 >1k 0.011093623625932908 5.7400263227940425 >5k 1.0673581180513263E-4 0.2513964977793276 >10k+ 4.181740702040581E-5 0.2504051124241866 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21284 0.14915972908769595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14473 0.10142777481141814 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.40968215115745E-5 0.0 0.0 2 8.40968215115745E-5 0.0 0.0 3 9.811295843017024E-5 0.0 0.0 4 9.811295843017024E-5 0.0 0.0 5 9.811295843017024E-5 0.0 0.0 6 9.811295843017024E-5 0.0 0.0 7 1.1212909534876599E-4 0.0 0.0 8 1.1913716380806386E-4 0.0 0.0 9 1.2614523226736174E-4 0.0 0.0 10-11 1.5417750610455323E-4 0.0 0.0 12-13 2.1024205377893624E-4 0.0 0.0 14-15 2.8733080683121287E-4 0.0 0.0 16-17 3.8193973103173415E-4 0.0 0.0 18-19 4.8706075792120224E-4 0.0 0.0 20-21 6.13205990188564E-4 0.0 0.0 22-23 8.094319070489045E-4 0.0 7.008068459297874E-6 24-25 9.916416869906492E-4 7.008068459297874E-6 7.008068459297874E-6 26-27 0.0011843635696213409 7.008068459297874E-6 7.008068459297874E-6 28-29 0.00145067017107466 7.008068459297874E-6 7.008068459297874E-6 30-31 0.001727488875216926 7.008068459297874E-6 7.008068459297874E-6 32-33 0.00204285195588533 7.008068459297874E-6 7.008068459297874E-6 34-35 0.0024353037896060114 1.0512102688946812E-5 7.008068459297874E-6 36-37 0.003146622738224745 1.4016136918595749E-5 7.008068459297874E-6 38-39 0.003949046576814352 2.1024205377893624E-5 7.008068459297874E-6 40-41 0.00484607933960448 3.5040342296489374E-5 7.008068459297874E-6 42-43 0.006244188997234406 3.5040342296489374E-5 7.008068459297874E-6 44-45 0.007824508434806077 3.5040342296489374E-5 7.008068459297874E-6 46-47 0.00975172726111299 3.5040342296489374E-5 7.008068459297874E-6 48-49 0.012127462468814972 3.5040342296489374E-5 7.008068459297874E-6 50-51 0.015137427872083407 3.5040342296489374E-5 7.008068459297874E-6 52-53 0.019139034962342497 3.5040342296489374E-5 7.008068459297874E-6 54-55 0.0239080255488947 3.5040342296489374E-5 7.008068459297874E-6 56-57 0.030057605621928583 3.5040342296489374E-5 7.008068459297874E-6 58-59 0.03778400109830449 3.5040342296489374E-5 7.008068459297874E-6 60-61 0.0469645707799847 3.5040342296489374E-5 7.008068459297874E-6 62-63 0.058342169923654805 3.8544376526138314E-5 7.008068459297874E-6 64-65 0.07226019388382038 4.204841075578725E-5 1.0512102688946812E-5 66-67 0.08963669962864945 4.204841075578725E-5 1.4016136918595749E-5 68-69 0.11147033691359198 4.204841075578725E-5 1.4016136918595749E-5 70-71 0.13839183189998477 4.204841075578725E-5 1.4016136918595749E-5 72-73 0.1702154707736564 4.204841075578725E-5 1.4016136918595749E-5 74-75 0.20905418617508525 4.204841075578725E-5 1.4016136918595749E-5 76-77 0.25655487419220624 4.204841075578725E-5 1.4016136918595749E-5 78-79 0.3160919197881713 5.256051344473406E-5 1.4016136918595749E-5 80-81 0.38794914973558203 5.6064547674382994E-5 1.4016136918595749E-5 82-83 0.47507345682157326 5.6064547674382994E-5 1.4016136918595749E-5 84-85 0.5804397661071168 5.6064547674382994E-5 1.4016136918595749E-5 86-87 0.705642413166703 5.6064547674382994E-5 1.4016136918595749E-5 88-89 0.8533970245283097 6.307261613368087E-5 1.4016136918595749E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 108280 0.0 63.959652 1 TTTGTGT 113595 0.0 61.02142 2 ACTTTGT 33895 0.0 55.925194 1 TTGTGTT 129870 0.0 54.83206 3 TGTGTTT 138735 0.0 51.54778 4 GTGTTTG 151640 0.0 47.175026 5 AACTTTG 15305 0.0 44.31786 2 TAACTTT 15380 0.0 43.57103 1 TTTGAGG 45030 0.0 33.120277 8 GTTTGAG 119010 0.0 32.68793 7 TTGAGGG 21395 0.0 32.13555 9 TGTTTGA 227570 0.0 31.441074 6 TTTGAGT 46500 0.0 29.470186 3 TTTGAGC 32280 0.0 27.285906 8 TTGAGTC 16455 0.0 26.044708 4 GTTTGAC 45900 0.0 25.127125 7 TTGAGGC 10150 0.0 24.887144 9 GTTTGAA 24390 0.0 23.265974 2 TTTGAGA 19940 0.0 22.883698 8 TTGAGGT 15395 0.0 22.792618 9 >>END_MODULE