FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30301.34100000035c7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30301.34100000035c7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14269267
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212840.14915972908769595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144730.10142777481141814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1082800.063.9596521
TTTGTGT1135950.061.021422
ACTTTGT338950.055.9251941
TTGTGTT1298700.054.832063
TGTGTTT1387350.051.547784
GTGTTTG1516400.047.1750265
AACTTTG153050.044.317862
TAACTTT153800.043.571031
TTTGAGG450300.033.1202778
GTTTGAG1190100.032.687937
TTGAGGG213950.032.135559
TGTTTGA2275700.031.4410746
TTTGAGT465000.029.4701863
TTTGAGC322800.027.2859068
TTGAGTC164550.026.0447084
GTTTGAC459000.025.1271257
TTGAGGC101500.024.8871449
GTTTGAA243900.023.2659742
TTTGAGA199400.022.8836988
TTGAGGT153950.022.7926189
Produced by FastQC (version 0.11.2)