##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30295.34100000035c39.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13629433 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74336298509263 33.0 33.0 33.0 33.0 33.0 2 32.756415105455964 33.0 33.0 33.0 33.0 33.0 3 32.790743679505965 33.0 33.0 33.0 33.0 33.0 4 36.65227599710127 37.0 37.0 37.0 37.0 37.0 5 36.690016378524334 37.0 37.0 37.0 37.0 37.0 6 36.66811744846613 37.0 37.0 37.0 37.0 37.0 7 36.6346225114427 37.0 37.0 37.0 37.0 37.0 8 36.62749602276192 37.0 37.0 37.0 37.0 37.0 9 36.61702896958369 37.0 37.0 37.0 37.0 37.0 10-11 36.58413391077971 37.0 37.0 37.0 37.0 37.0 12-13 36.561285308053534 37.0 37.0 37.0 37.0 37.0 14-15 38.65463629338066 40.0 37.0 40.0 37.0 40.0 16-17 38.59830544674896 40.0 37.0 40.0 37.0 40.0 18-19 38.53826234004012 40.0 37.0 40.0 37.0 40.0 20-21 38.51066361307913 40.0 37.0 40.0 37.0 40.0 22-23 38.46529048567171 40.0 37.0 40.0 37.0 40.0 24-25 38.4119330202511 40.0 37.0 40.0 37.0 40.0 26-27 38.321694159984496 40.0 37.0 40.0 37.0 40.0 28-29 38.23701565575031 40.0 37.0 40.0 37.0 40.0 30-31 38.18616089898971 40.0 37.0 40.0 37.0 40.0 32-33 38.10669501071688 40.0 37.0 40.0 37.0 40.0 34-35 38.020694661325976 40.0 37.0 40.0 37.0 40.0 36-37 37.92442136074186 40.0 37.0 40.0 35.0 40.0 38-39 37.81691054205997 40.0 37.0 40.0 33.0 40.0 40-41 37.70525703453695 40.0 37.0 40.0 33.0 40.0 42-43 37.566978795082676 40.0 37.0 40.0 33.0 40.0 44-45 37.459121373574376 40.0 37.0 40.0 33.0 40.0 46-47 37.29207873137496 40.0 37.0 40.0 33.0 40.0 48-49 37.1144229184002 37.0 37.0 40.0 33.0 40.0 50-51 36.94842683477735 37.0 37.0 40.0 33.0 40.0 52-53 36.73474916381335 37.0 37.0 40.0 33.0 40.0 54-55 36.51737111881323 37.0 37.0 40.0 33.0 40.0 56-57 36.307464074257524 37.0 37.0 40.0 33.0 40.0 58-59 36.07070818720045 37.0 37.0 40.0 33.0 40.0 60-61 35.80093280476157 37.0 37.0 40.0 33.0 40.0 62-63 35.552195054629195 37.0 33.0 37.0 33.0 40.0 64-65 35.260978281341565 37.0 33.0 37.0 33.0 40.0 66-67 34.97015657217729 37.0 33.0 37.0 27.0 40.0 68-69 34.68329295136489 37.0 33.0 37.0 27.0 38.5 70-71 34.4237836599659 37.0 33.0 37.0 27.0 37.0 72-73 34.214650638805004 37.0 33.0 37.0 27.0 37.0 74-75 33.91345043480533 37.0 33.0 37.0 27.0 37.0 76-77 31.02729222118044 33.0 30.0 35.0 24.5 37.0 78-79 33.61876502859657 35.0 33.0 37.0 27.0 37.0 80-81 34.079676975557234 37.0 33.0 37.0 27.0 37.0 82-83 34.0245582116292 37.0 33.0 37.0 27.0 37.0 84-85 33.952822358787785 37.0 33.0 37.0 27.0 37.0 86-87 33.82386449971911 37.0 33.0 37.0 27.0 37.0 88-89 33.71908798407094 37.0 33.0 37.0 27.0 37.0 90-91 33.64274999554273 37.0 33.0 37.0 27.0 37.0 92-93 33.549080728449965 37.0 33.0 37.0 27.0 37.0 94-95 33.41162614761744 37.0 33.0 37.0 27.0 37.0 96-97 33.30279707893938 37.0 33.0 37.0 27.0 37.0 98-99 33.14083047328528 37.0 33.0 37.0 27.0 37.0 100-101 31.360503918248103 33.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1222901597997037 2306 2 0.11581191162860449 2306 3 0.08930966477107916 2306 4 0.046401826390535916 2306 5 0.044931349801565545 2306 6 0.08212506378987428 2306 7 0.08481869932964514 2306 8 0.10762434677643284 2306 9 0.09188321561457968 2306 10-11 0.13135747203480008 2306 12-13 0.10407618708494226 2306 14-15 0.17830837747453643 2306 16-17 0.20329104694989297 2306 18-19 0.19595122324454195 2306 20-21 0.16204808960763728 2306 22-23 0.14530257859110662 2306 24-25 0.14601158828461536 2306 26-27 0.2278209288134292 2306 28-29 0.17681511123495142 2306 30-31 0.1863271947641465 2306 32-33 0.16477599086685046 2306 34-35 0.15244418301531937 2306 36-37 0.1848495561885244 2306 38-39 0.18682335347909174 2306 40-41 0.13159533351550579 2306 42-43 0.12969059630090385 2306 44-45 0.11159172460771316 2306 46-47 0.09732328588693662 2306 48-49 0.02842438428412919 2306 50-51 0.0445789094408795 2306 52-53 0.009101409759857404 2306 54-55 0.01024578168107837 2306 56-57 0.07440269028549551 2306 58-59 -0.041825747484764975 2306 60-61 -0.03487396283852462 2306 62-63 -0.03180936046749849 2306 64-65 -0.04749606281926333 2306 66-67 -0.14378260426748568 2306 68-69 -0.20335947629686757 2306 70-71 -0.196294823358798 2306 72-73 -0.19712802955286435 2306 74-75 -0.2116371810891451 2306 76-77 -0.4593285045607587 2306 78-79 -0.07123364092699802 2306 80-81 0.02346865116840746 2306 82-83 -0.0048037039729109665 2306 84-85 -0.1468266647302201 2306 86-87 -0.19876394275799925 2306 88-89 -0.0396520356359531 2306 90-91 -0.14468620246771025 2306 92-93 -0.123084975106174 2306 94-95 -0.17535061822160003 2306 96-97 -0.2966439469129156 2306 98-99 -0.24445365798590757 2306 100-101 -0.3860958767317406 2307 1 0.09326120624366041 2307 2 0.10681255242482735 2307 3 0.090609775908014 2307 4 0.05093291129054478 2307 5 0.06654836364817385 2307 6 0.09508888976413488 2307 7 0.07562472855163804 2307 8 0.1052175166542284 2307 9 0.1017619024973726 2307 10-11 0.10088409178140267 2307 12-13 0.1368478378499276 2307 14-15 0.18094364264603513 2307 16-17 0.17620595630462077 2307 18-19 0.15951683012139029 2307 20-21 0.1648284970667362 2307 22-23 0.15256154277307843 2307 24-25 0.10635303156340115 2307 26-27 0.14394307163401976 2307 28-29 0.1225714649018883 2307 30-31 0.1251737007960685 2307 32-33 0.09490136238770219 2307 34-35 0.1281444395239575 2307 36-37 0.10507388819091545 2307 38-39 0.06240009901635801 2307 40-41 0.0497559891729793 2307 42-43 0.10136357544281793 2307 44-45 0.09263797098116555 2307 46-47 0.041738109386066924 2307 48-49 -0.038468383994406 2307 50-51 -0.02194788739067377 2307 52-53 -0.15434690106706483 2307 54-55 -0.08301977945296102 2307 56-57 -0.1301159585711531 2307 58-59 -0.10347029671963526 2307 60-61 -0.14408540649512958 2307 62-63 -0.15176300752890626 2307 64-65 -0.17845327274406486 2307 66-67 -0.3213311912295467 2307 68-69 -0.27927347071265984 2307 70-71 -0.2888285552738239 2307 72-73 -0.34965758938927394 2307 74-75 -0.39987992827374796 2307 76-77 -0.6979625665135423 2307 78-79 -0.243681569309949 2307 80-81 -0.17625844206136065 2307 82-83 -0.25745681611704185 2307 84-85 -0.21531745527897783 2307 86-87 -0.1554021960521581 2307 88-89 -0.2758843311704169 2307 90-91 -0.283397059106683 2307 92-93 -0.285140095651677 2307 94-95 -0.2664667507357166 2307 96-97 -0.556767944413231 2307 98-99 -0.3899373839030389 2307 100-101 -0.4220400723175288 2201 1 0.08116705721922557 2201 2 0.0969999608881551 2201 3 0.06405056672603848 2201 4 -0.03643888297684583 2201 5 0.012810987820920161 2201 6 0.03862169947219485 2201 7 0.08753172449067392 2201 8 0.08965613508024717 2201 9 0.06643319083016053 2201 10-11 0.07731404976542677 2201 12-13 0.08743521472121074 2201 14-15 0.1293053426522448 2201 16-17 0.1297455821984883 2201 18-19 0.1380187849077359 2201 20-21 0.09691644372855279 2201 22-23 0.07864713946170099 2201 24-25 0.15342449947764436 2201 26-27 0.18748459816570318 2201 28-29 0.14240128910453365 2201 30-31 0.1474404660230988 2201 32-33 0.1394970958814028 2201 34-35 0.13390055879331442 2201 36-37 0.10229922512467482 2201 38-39 0.10537883500554557 2201 40-41 0.0990073644251126 2201 42-43 0.1468168768018785 2201 44-45 0.10502371464761495 2201 46-47 0.10092925934601027 2201 48-49 0.1386964564274038 2201 50-51 0.07646591982891238 2201 52-53 0.014298990675058576 2201 54-55 -0.02122620815141829 2201 56-57 0.0251207500478543 2201 58-59 -0.03965779874519626 2201 60-61 0.047807745211692065 2201 62-63 0.06088748364988561 2201 64-65 -0.03562879036820732 2201 66-67 -0.10360512097176411 2201 68-69 -0.13201865366078636 2201 70-71 -0.10726754031414032 2201 72-73 -0.20353034319437313 2201 74-75 -0.18900157001102968 2201 76-77 -0.04772361159942662 2201 78-79 -0.04537082559864558 2201 80-81 -0.04613381534163352 2201 82-83 -0.07923199505432876 2201 84-85 -0.03521376458692771 2201 86-87 -0.0191853599205416 2201 88-89 3.0568849126666464E-5 2201 90-91 -0.05620877373127797 2201 92-93 -0.08074779759044048 2201 94-95 -0.0863217806254255 2201 96-97 -0.2558210019073499 2201 98-99 -0.09978158084422972 2201 100-101 -0.1410764909279898 1101 1 0.09050465657938389 1101 2 0.08041594348495096 1101 3 0.04147559696455261 1101 4 -0.0016078856555026277 1101 5 0.0015536993259814835 1101 6 0.05293000503001366 1101 7 0.07439152417104111 1101 8 0.037130337967340665 1101 9 0.013905999902192434 1101 10-11 0.05561743490907389 1101 12-13 0.07106209124067675 1101 14-15 0.09050653317849822 1101 16-17 0.08427228640270101 1101 18-19 0.12349556718192645 1101 20-21 0.10615502046799463 1101 22-23 0.08000818663742137 1101 24-25 0.10693398333496162 1101 26-27 0.12180081635870721 1101 28-29 0.1277890039497649 1101 30-31 0.03349127250521633 1101 32-33 0.08206202629293813 1101 34-35 0.0940739827327377 1101 36-37 0.022987328331780077 1101 38-39 0.02757574301737975 1101 40-41 0.003545782409908327 1101 42-43 0.06381755845683301 1101 44-45 0.08298620173840021 1101 46-47 0.07940935024603846 1101 48-49 0.052285449318127064 1101 50-51 -0.0018624992918390149 1101 52-53 -0.0974744795848963 1101 54-55 -0.16081967128934593 1101 56-57 -0.09751503415841256 1101 58-59 -0.01922274971990845 1101 60-61 0.01661146470775776 1101 62-63 -0.11257682348549025 1101 64-65 -0.03104784955592521 1101 66-67 -0.06133697789502435 1101 68-69 -0.14383777312835377 1101 70-71 -0.16262003407027237 1101 72-73 -0.29813932941824817 1101 74-75 -0.23953366464504455 1101 76-77 -0.47927007225881724 1101 78-79 -0.04321824143508479 1101 80-81 0.07695720827147312 1101 82-83 0.0431434765458647 1101 84-85 -0.0011905227913331373 1101 86-87 0.0036772915054470445 1101 88-89 0.02486488195064851 1101 90-91 0.04276751144935531 1101 92-93 -0.05319614939838857 1101 94-95 -0.13247737535894544 1101 96-97 -0.11869327700882337 1101 98-99 -0.01112147669996233 1101 100-101 0.028010235659134963 2304 1 0.08678166327260328 2304 2 0.07344304066662488 2304 3 0.04456894919668741 2304 4 -0.004328377261394678 2304 5 -0.004061383697205656 2304 6 0.020255721877070698 2304 7 0.04360753066125511 2304 8 0.05103434917609917 2304 9 0.009816544411336281 2304 10-11 0.05855284298718999 2304 12-13 0.03339563222881736 2304 14-15 0.06588780280599593 2304 16-17 0.04009920081804097 2304 18-19 0.05866095860715603 2304 20-21 0.049299797028957926 2304 22-23 0.0713973644448842 2304 24-25 0.016415307857329253 2304 26-27 0.04705037339954288 2304 28-29 0.07986097375822254 2304 30-31 0.05915705230034973 2304 32-33 0.09218567913345765 2304 34-35 0.0494957988464364 2304 36-37 0.06968856769611875 2304 38-39 0.09133589196328984 2304 40-41 -0.052271997451917684 2304 42-43 -0.012211705628473624 2304 44-45 0.014948735112064071 2304 46-47 0.05997961328640855 2304 48-49 0.02657176302822961 2304 50-51 0.005091547759242587 2304 52-53 -0.043541098217431795 2304 54-55 -0.11417308460336528 2304 56-57 -0.04507759547903589 2304 58-59 -0.014425534066660362 2304 60-61 -0.05692913265915678 2304 62-63 -0.055127170859123 2304 64-65 -0.08094931231917712 2304 66-67 -0.15269366519862615 2304 68-69 -0.10240743460060031 2304 70-71 -0.11376798537131094 2304 72-73 -0.07072804559856394 2304 74-75 -0.18785689600823474 2304 76-77 -0.4734306796539336 2304 78-79 -0.13962192091898373 2304 80-81 0.04310591040761835 2304 82-83 0.026665037401812697 2304 84-85 0.025816319753445782 2304 86-87 0.048045320931471736 2304 88-89 0.15938246956218904 2304 90-91 0.0793149753838378 2304 92-93 -0.09391724270331281 2304 94-95 -0.1460236956496317 2304 96-97 -0.08585297800978964 2304 98-99 -0.009724043020206352 2304 100-101 0.10889448138455293 2202 1 0.04245810604332689 2202 2 0.05170008241215385 2202 3 0.03510877490418807 2202 4 -0.022494509121912643 2202 5 -0.008154215494123207 2202 6 0.04546119729306497 2202 7 0.028961134530852917 2202 8 0.0484354073050568 2202 9 0.031303888773727806 2202 10-11 0.016481052741418978 2202 12-13 0.028383857429886916 2202 14-15 0.03269095864366989 2202 16-17 0.02332462755513376 2202 18-19 0.031468373718333 2202 20-21 0.0024308975450253456 2202 22-23 0.09736326748981128 2202 24-25 0.018576379083434347 2202 26-27 0.019283782063006072 2202 28-29 -0.015886261586132377 2202 30-31 0.027715821289000075 2202 32-33 0.05146870496331957 2202 34-35 0.008303722461775465 2202 36-37 0.06518264868159918 2202 38-39 0.06203847632176007 2202 40-41 -0.0023826309273786705 2202 42-43 0.04905018433177588 2202 44-45 0.06295510287288408 2202 46-47 0.11052764415921246 2202 48-49 0.05300832171647585 2202 50-51 0.02983323676968297 2202 52-53 0.05551668462931758 2202 54-55 0.04452897676008405 2202 56-57 0.02924215116662765 2202 58-59 0.027005577496616695 2202 60-61 0.11336991746220093 2202 62-63 0.0957318498934896 2202 64-65 -0.042678392456231506 2202 66-67 -0.08025205317406403 2202 68-69 -0.02531438556874832 2202 70-71 -0.056034716759796765 2202 72-73 -0.226456137061966 2202 74-75 -0.18216004829091048 2202 76-77 -0.3304447270344717 2202 78-79 0.0016110105017403953 2202 80-81 0.07781774501858507 2202 82-83 0.03607184531600893 2202 84-85 0.013570895265814897 2202 86-87 0.03105583006018975 2202 88-89 0.06376685949702221 2202 90-91 0.003398717817397312 2202 92-93 0.12498681833963587 2202 94-95 -0.020460432260911432 2202 96-97 -0.10790537924889776 2202 98-99 0.0294411906555041 2202 100-101 0.18607076255513988 1102 1 0.058521429996055474 1102 2 0.0670151160921506 1102 3 0.03701307110983265 1102 4 -0.04514223666530626 1102 5 -0.05637146683687888 1102 6 0.04043177926721597 1102 7 -0.0015926505443388805 1102 8 0.021279957840661723 1102 9 0.01926773650629343 1102 10-11 0.04781805846662479 1102 12-13 0.038749467494071155 1102 14-15 0.06474859741994266 1102 16-17 0.04417777090861108 1102 18-19 0.02321657687802059 1102 20-21 0.022520290877579896 1102 22-23 0.0714025797819744 1102 24-25 0.024786647839654563 1102 26-27 0.028172560362698107 1102 28-29 0.026061666960082164 1102 30-31 -0.04701441743725354 1102 32-33 0.0646112662860503 1102 34-35 0.01078365906019485 1102 36-37 -0.020566863810927316 1102 38-39 0.005646675370023502 1102 40-41 0.04266350395563023 1102 42-43 0.024644451282981095 1102 44-45 0.0013897050886910733 1102 46-47 -0.05340321253940061 1102 48-49 -0.045255901574655866 1102 50-51 -0.006965479589091217 1102 52-53 -0.024148699251405503 1102 54-55 -0.12197541978351012 1102 56-57 -0.02580876902698037 1102 58-59 -0.11779438682302157 1102 60-61 -0.039819182029376066 1102 62-63 0.006242273362580875 1102 64-65 -0.061153495503589284 1102 66-67 -0.15350943324303046 1102 68-69 -0.07193896348876905 1102 70-71 -0.18680141573969422 1102 72-73 -0.17513788756203752 1102 74-75 -0.18276926264147164 1102 76-77 -0.451908287315991 1102 78-79 -0.1327528717518689 1102 80-81 -0.020661180590586525 1102 82-83 -0.01471346868417811 1102 84-85 -0.0542691720114874 1102 86-87 -0.035995391718692815 1102 88-89 -0.04981326970333555 1102 90-91 -0.11725330183558924 1102 92-93 -0.1464162449459394 1102 94-95 -0.3021120895335514 1102 96-97 -0.36337017702454233 1102 98-99 -0.3211450751377143 1102 100-101 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-0.14842405106232803 1213 46-47 -0.35182548641738265 1213 48-49 -0.1562969044878315 1213 50-51 -0.1383952176352352 1213 52-53 -0.0764413678611433 1213 54-55 0.00956907841467114 1213 56-57 0.003189932022365838 1213 58-59 -0.009126572966295043 1213 60-61 0.045341324105059755 1213 62-63 0.05336127033471172 1213 64-65 0.10162177894562774 1213 66-67 0.1350936146521846 1213 68-69 0.19216340514706332 1213 70-71 0.14562189890747135 1213 72-73 0.1608463763488075 1213 74-75 0.2318234377671189 1213 76-77 0.5799272364320451 1213 78-79 0.18721806849775646 1213 80-81 4.1570532672352556E-4 1213 82-83 0.036804784913258004 1213 84-85 0.048784480917909434 1213 86-87 0.019339607503653156 1213 88-89 0.07788092803834701 1213 90-91 0.10568058937253966 1213 92-93 0.2114782946910907 1213 94-95 0.2631587971446123 1213 96-97 0.23123359524409892 1213 98-99 0.33462950551847115 1213 100-101 0.5882565744606758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 22.0 8 401.0 9 1129.0 10 851.0 11 322.0 12 297.0 13 508.0 14 1289.0 15 2914.0 16 5601.0 17 8955.0 18 12444.0 19 16348.0 20 20980.0 21 26105.0 22 32765.0 23 40052.0 24 49354.0 25 61035.0 26 75580.0 27 95245.0 28 120367.0 29 152531.0 30 198074.0 31 259457.0 32 350914.0 33 496535.0 34 771191.0 35 1441464.0 36 3202641.0 37 5024682.0 38 1159339.0 39 39.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.84208350788914 24.662285028188773 32.01869729620833 23.476934167713758 2 24.541006217940247 24.80681331351055 30.60746547563644 20.044714992912766 3 21.54605404348075 22.905054084054708 34.89186967645683 20.657022196007713 4 21.55495020456873 23.27962964691635 34.04192278949969 21.123497359015236 5 25.525808740539684 22.36280848953878 31.90239828758834 20.208984482333197 6 26.519635849855238 22.79702317770666 30.967678552732163 19.71566241970594 7 25.46494047111131 25.823972281165332 28.822057381257167 19.8890298664662 8 24.65271299253608 23.94165626699218 30.95717187941714 20.448458861054604 9 22.442753121131304 23.24740875133984 33.54358174694428 20.766256380584576 10-11 25.03078016816917 24.037347741300064 30.070741284326797 20.861130806203967 12-13 24.633533911498738 26.086485035731126 28.095746903044315 21.184234149725818 14-15 24.578687824679957 24.64630277100585 29.018378895068103 21.75663050924609 16-17 24.294033361475858 24.658956832613654 28.9137449811742 22.13326482473629 18-19 23.655826328211894 24.933014454819947 29.32854580230887 22.08261341465929 20-21 22.95197548130011 25.36660854404389 29.47280326807406 22.20861270658194 22-23 22.944677889388355 25.266348203920145 29.346371195338794 22.442602711352702 24-25 23.165384062565188 25.444139899290015 28.89105511579242 22.499420922352385 26-27 23.011045287063666 25.34012236605881 29.164430391198227 22.4844019556793 28-29 23.180685506139547 25.44938223035397 28.718124224243226 22.651808039263262 30-31 22.962850597145515 25.24880176529824 28.808588770252598 22.979758867303644 32-33 22.83359843362523 25.694223670199634 28.51149053669364 22.9606873594815 34-35 22.6927546190104 25.790442467876908 28.343341410842694 23.173461502269998 36-37 22.782474101657698 25.98680183578208 28.565232405208285 22.665491657351936 38-39 23.098770373301665 26.027180997758283 28.14542358816511 22.728625040774943 40-41 22.73455849525132 26.60186094813722 28.183795640206714 22.479784916404746 42-43 22.903738565402325 26.49561270748535 27.994096984618917 22.60655174249341 44-45 22.736219876658907 26.123196919279103 28.634643325737553 22.505939878324437 46-47 22.71263366920123 26.313678868732122 28.576843428740478 22.396844033326172 48-49 22.689104608606065 25.95383411201685 28.671893010220394 22.685168269156694 50-51 22.6541019673394 26.252384064899026 28.521624613686015 22.571889354075562 52-53 22.7313916422838 26.72814786662051 28.128522263586238 22.411938227509452 54-55 22.682516407623453 26.513671579386283 28.330590060955206 22.473221952035058 56-57 22.779564701355692 26.736270701541653 28.18324948788978 22.300915109212877 58-59 22.67947215387137 26.714552674694463 28.23156602410953 22.374409147324638 60-61 22.823054416049175 26.65133599721296 27.837023548385265 22.6885860383526 62-63 22.68425617551375 26.908695938964165 27.878165036320425 22.52888284920166 64-65 23.11846145137 26.965115523554044 27.478156088792023 22.438266936283934 66-67 23.486369405064643 26.97539151201439 27.0409899311068 22.497249151814167 68-69 23.225962230917073 27.158265943149136 27.10635615960809 22.5094156663257 70-71 23.35683713481025 27.344294240400068 27.11931654683254 22.179552077957144 72-73 23.466652899685158 27.093918626718644 27.045840599342164 22.393587874254035 74-75 23.27850396803834 27.18667162079069 27.193881199425864 22.340943211745103 76-77 23.296441132678538 27.137286637174775 27.18483830250499 22.381433927641698 78-79 23.42252124003057 26.984532949733747 27.17980962789419 22.413136182341493 80-81 23.406714033196682 27.007895970714245 27.24419909204775 22.34119090404132 82-83 23.112860397271927 27.150917423232805 27.224615922375662 22.511606257119606 84-85 22.903467551908925 27.391394675586607 27.116607003176043 22.588530769328425 86-87 22.85955027282175 27.133409142223414 27.137687058876537 22.869353526078303 88-89 22.53590538212455 27.513763829183407 27.058366414037042 22.891964374655 90-91 22.599877318676466 27.529493010787082 27.11590140585104 22.754728264685415 92-93 22.64806405969463 27.87519389642602 26.833793752479902 22.64294829139945 94-95 22.56021534603226 28.10865119452367 26.6633096828909 22.66782377655317 96-97 22.589384666949904 28.148230155228966 26.62145836744462 22.640926810376506 98-99 22.79718717894255 28.076786336308718 26.599806471945698 22.52622001280303 100-101 23.14865080444389 28.165454289101135 26.10204168240932 22.58385322404565 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 95618.0 1 52832.5 2 15751.0 3 19071.0 4 18080.0 5 13905.0 6 7743.0 7 7888.0 8 9164.0 9 9165.0 10 7389.0 11 5064.0 12 3474.0 13 2787.0 14 2533.5 15 2449.5 16 2501.5 17 2653.0 18 2838.5 19 3018.5 20 3248.0 21 3535.0 22 3938.0 23 4467.0 24 5013.0 25 5631.5 26 6365.0 27 7471.0 28 8821.5 29 10130.5 30 12306.0 31 17139.5 32 48152.5 33 96162.5 34 146411.5 35 235563.0 36 309389.0 37 430123.0 38 560179.0 39 534859.5 40 481452.5 41 475920.0 42 509656.5 43 632435.5 44 734131.5 45 783028.5 46 790488.0 47 711152.5 48 642790.0 49 628422.5 50 584250.5 51 514424.0 52 473750.0 53 449667.0 54 416251.5 55 388908.0 56 384182.0 57 347092.0 58 282634.5 59 221846.0 60 168517.5 61 120163.0 62 75881.0 63 44328.0 64 26035.0 65 16177.0 66 10366.5 67 6969.5 68 5261.0 69 4082.5 70 3091.0 71 2351.0 72 1886.5 73 1548.0 74 1212.0 75 857.5 76 638.5 77 561.5 78 509.5 79 427.0 80 302.5 81 188.5 82 126.0 83 93.5 84 67.0 85 50.5 86 45.5 87 43.5 88 31.0 89 21.0 90 21.0 91 21.0 92 14.0 93 8.0 94 6.5 95 7.5 96 7.5 97 4.5 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13716638102260012 2 0.0 3 0.0 4 0.17953791621412277 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.914530780554115E-4 12-13 0.0 14-15 3.6685311854132155E-4 16-17 0.0 18-19 0.0 20-21 0.0035841549681487122 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 5.869649896661145E-5 32-33 0.0 34-35 1.87095090456074E-4 36-37 2.751398389059912E-4 38-39 0.009336411866876634 40-41 0.007758943457148951 42-43 9.3547545228037E-4 44-45 0.00114091319866351 46-47 0.0019443215282690044 48-49 1.394041850457022E-4 50-51 0.0010088460759886343 52-53 0.01995680964864789 54-55 0.01804550490104761 56-57 0.021805749366096156 58-59 0.0417478848900024 60-61 0.015154702326941995 62-63 0.002377208208147764 64-65 0.007179315529853663 66-67 0.013338779390162451 68-69 0.028398099906283704 70-71 0.01201443963222828 72-73 0.019267125785790207 74-75 0.012689449370344313 76-77 0.02348960518020082 78-79 0.009783972671497046 80-81 0.007065591063105853 82-83 0.0018672823733753266 84-85 0.020030180272356157 86-87 0.009567529331557667 88-89 0.01302695423940233 90-91 0.04646194746325838 92-93 0.03588557205571207 94-95 0.03012964662579874 96-97 0.027073760148349533 98-99 0.033273577851697864 100-101 0.2431942693434129 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3629433E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.023519191074875 #Duplication Level Percentage of deduplicated Percentage of total 1 78.61698617278417 13.383377728568597 2 9.184822907174455 3.12716018053817 3 3.3343902834603805 1.7028917094306415 4 1.4898007594763862 1.0144660727929675 5 0.9448355196695358 0.8042212800751773 6 0.9189382875324191 0.9386138143932972 7 0.6593134032311763 0.7856684060987172 8 0.4785855348131813 0.651776802917042 9 0.3692450429123461 0.5657265066804832 >10 2.8374935653061995 9.685976835110678 >50 0.4602372477583154 5.601764090099421 >100 0.5377035166673385 19.228093133870765 >500 0.09034538448714365 10.728953758483838 >1k 0.07074468918733205 22.196511877543205 >5k 0.0052374716441741815 6.190820507678449 >10k+ 0.0013202138951473785 3.393977295718529 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60890 0.4467537277596214 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43556 0.31957308862371603 No Hit CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC 21695 0.15917756813507944 No Hit TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC 16200 0.11886041040738819 No Hit TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15984 0.11727560493528968 No Hit TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT 14415 0.105763754075463 No Hit CCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA 14299 0.10491265484044714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1005593556239646E-4 0.0 0.0 2 1.1005593556239646E-4 0.0 0.0 3 1.173929979332229E-4 0.0 0.0 4 1.2473006030404933E-4 0.0 0.0 5 1.3940418504570219E-4 0.0 0.0 6 1.3940418504570219E-4 0.0 0.0 7 1.4674124741652863E-4 0.0 0.0 8 1.9076362164148722E-4 0.0 0.0 9 2.127748087539665E-4 0.0 0.0 10-11 3.081566195747101E-4 0.0 0.0 12-13 4.915831788453709E-4 0.0 0.0 14-15 8.070768607909074E-4 0.0 0.0 16-17 0.0013463509450466502 0.0 0.0 18-19 0.0021020683692417725 0.0 0.0 20-21 0.0029458305418868123 0.0 0.0 22-23 0.003940002493133794 0.0 0.0 24-25 0.005062573035870238 0.0 0.0 26-27 0.006463951948698086 0.0 0.0 28-29 0.00789467911100924 0.0 0.0 30-31 0.009450136333624444 0.0 0.0 32-33 0.011383452268337209 3.6685311854132158E-6 0.0 34-35 0.01333511085897704 7.3370623708264315E-6 0.0 36-37 0.0151840505764253 7.3370623708264315E-6 0.0 38-39 0.017564927315758476 7.3370623708264315E-6 0.0 40-41 0.020437387233937024 7.3370623708264315E-6 0.0 42-43 0.023614335240504866 7.3370623708264315E-6 0.0 44-45 0.027257186707620194 7.3370623708264315E-6 0.0 46-47 0.031252217168535185 1.1005593556239648E-5 0.0 48-49 0.03597361680416199 1.4674124741652863E-5 0.0 50-51 0.04151676742532136 1.4674124741652863E-5 0.0 52-53 0.047991724967575686 1.4674124741652863E-5 0.0 54-55 0.055640612489162244 1.4674124741652863E-5 0.0 56-57 0.06486696842047648 1.834265592706608E-5 0.0 58-59 0.07582853960249117 2.567971829789251E-5 0.0 60-61 0.08933607142718263 2.9348249483305726E-5 0.0 62-63 0.10555097926670903 2.9348249483305726E-5 0.0 64-65 0.12495017217517412 2.9348249483305726E-5 0.0 66-67 0.14860852978990396 2.9348249483305726E-5 0.0 68-69 0.17698461850907518 2.9348249483305726E-5 0.0 70-71 0.2118540074264278 2.9348249483305726E-5 0.0 72-73 0.25484185585709984 2.9348249483305726E-5 0.0 74-75 0.3067919259737364 2.9348249483305726E-5 0.0 76-77 0.36861768204150536 2.9348249483305726E-5 0.0 78-79 0.4444425531128111 2.9348249483305726E-5 0.0 80-81 0.536284231339631 2.9348249483305726E-5 0.0 82-83 0.6489448240436708 2.9348249483305726E-5 0.0 84-85 0.7845630849060266 2.9348249483305726E-5 0.0 86-87 0.9490270064792865 3.301678066871894E-5 0.0 88-89 1.1459684346370094 3.668531185413216E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTGT 26140 0.0 58.926846 1 CTTTGTG 72275 0.0 57.195297 1 TTTGTGT 81645 0.0 50.779667 2 TTGTGTT 86160 0.0 49.714172 3 TGTGTTT 95275 0.0 45.16269 4 GTGTTTG 106745 0.0 40.299816 5 TTTGAGG 41875 0.0 31.41985 8 GTTTGAG 78335 0.0 30.082285 7 TTGAGTC 6170 0.0 29.912226 4 TTGAGGG 32320 0.0 29.602995 9 TGTTTGA 146230 0.0 29.369362 6 GTTTGAA 9760 0.0 27.779678 2 TCCGATC 450 0.0 27.433626 6 TTTGAGT 24295 0.0 27.273218 3 GTTTGAC 26185 0.0 27.126976 7 TGATTTT 9410 0.0 25.935577 5 TTGAGGC 4055 0.0 25.058489 9 TTTGAGA 10730 0.0 24.514755 3 TTTGACG 13045 0.0 24.022724 8 TGAGTGG 5110 0.0 23.880016 5 >>END_MODULE