FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30295.34100000035c39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30295.34100000035c39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13629433
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608900.4467537277596214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA435560.31957308862371603No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC216950.15917756813507944No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC162000.11886041040738819No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159840.11727560493528968No Hit
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT144150.105763754075463No Hit
CCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA142990.10491265484044714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT261400.058.9268461
CTTTGTG722750.057.1952971
TTTGTGT816450.050.7796672
TTGTGTT861600.049.7141723
TGTGTTT952750.045.162694
GTGTTTG1067450.040.2998165
TTTGAGG418750.031.419858
GTTTGAG783350.030.0822857
TTGAGTC61700.029.9122264
TTGAGGG323200.029.6029959
TGTTTGA1462300.029.3693626
GTTTGAA97600.027.7796782
TCCGATC4500.027.4336266
TTTGAGT242950.027.2732183
GTTTGAC261850.027.1269767
TGATTTT94100.025.9355775
TTGAGGC40550.025.0584899
TTTGAGA107300.024.5147553
TTTGACG130450.024.0227248
TGAGTGG51100.023.8800165