##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30292.34100000035c02.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15535871 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74278397394005 33.0 33.0 33.0 33.0 33.0 2 32.75759292800513 33.0 33.0 33.0 33.0 33.0 3 32.79247375316131 33.0 33.0 33.0 33.0 33.0 4 36.65411948901996 37.0 37.0 37.0 37.0 37.0 5 36.69243378758745 37.0 37.0 37.0 37.0 37.0 6 36.66928529465776 37.0 37.0 37.0 37.0 37.0 7 36.637401533522 37.0 37.0 37.0 37.0 37.0 8 36.62986001879135 37.0 37.0 37.0 37.0 37.0 9 36.61796329282085 37.0 37.0 37.0 37.0 37.0 10-11 36.585457294283664 37.0 37.0 37.0 37.0 37.0 12-13 36.56269651698318 37.0 37.0 37.0 37.0 37.0 14-15 38.663890650224886 40.0 37.0 40.0 37.0 40.0 16-17 38.60198527008882 40.0 37.0 40.0 37.0 40.0 18-19 38.53781059330372 40.0 37.0 40.0 37.0 40.0 20-21 38.51261503136837 40.0 37.0 40.0 37.0 40.0 22-23 38.46649502303411 40.0 37.0 40.0 37.0 40.0 24-25 38.41295428495769 40.0 37.0 40.0 37.0 40.0 26-27 38.32275818330366 40.0 37.0 40.0 37.0 40.0 28-29 38.24099611151509 40.0 37.0 40.0 37.0 40.0 30-31 38.188500181290124 40.0 37.0 40.0 37.0 40.0 32-33 38.11192047101833 40.0 37.0 40.0 37.0 40.0 34-35 38.02836995750029 40.0 37.0 40.0 37.0 40.0 36-37 37.93641988273461 40.0 37.0 40.0 35.0 40.0 38-39 37.840116656478415 40.0 37.0 40.0 33.0 40.0 40-41 37.73945110641045 40.0 37.0 40.0 33.0 40.0 42-43 37.61662635458289 40.0 37.0 40.0 33.0 40.0 44-45 37.52704119389251 40.0 37.0 40.0 33.0 40.0 46-47 37.38464592683603 40.0 37.0 40.0 33.0 40.0 48-49 37.227651864514065 40.0 37.0 40.0 33.0 40.0 50-51 37.08047292617195 37.0 37.0 40.0 33.0 40.0 52-53 36.89303274338465 37.0 37.0 40.0 33.0 40.0 54-55 36.69787184767433 37.0 37.0 40.0 33.0 40.0 56-57 36.509512051174994 37.0 37.0 40.0 33.0 40.0 58-59 36.28615270428031 37.0 37.0 40.0 33.0 40.0 60-61 36.022878440481385 37.0 37.0 40.0 33.0 40.0 62-63 35.77947525439674 37.0 37.0 40.0 33.0 40.0 64-65 35.48802233231726 37.0 35.0 37.0 33.0 40.0 66-67 35.198062020468626 37.0 33.0 37.0 30.0 40.0 68-69 34.90354811777209 37.0 33.0 37.0 27.0 40.0 70-71 34.62272543328919 37.0 33.0 37.0 27.0 38.5 72-73 34.38151925952526 37.0 33.0 37.0 27.0 37.0 74-75 34.05623328103072 37.0 33.0 37.0 27.0 37.0 76-77 31.254959731578616 33.0 30.0 35.0 24.5 37.0 78-79 33.72605365994607 35.0 33.0 37.0 27.0 37.0 80-81 34.135188332858846 37.0 33.0 37.0 27.0 37.0 82-83 34.06603794534597 37.0 33.0 37.0 27.0 37.0 84-85 33.98233694139196 37.0 33.0 37.0 27.0 37.0 86-87 33.848018208956546 37.0 33.0 37.0 27.0 37.0 88-89 33.73927441853759 37.0 33.0 37.0 27.0 37.0 90-91 33.65571862691188 37.0 33.0 37.0 27.0 37.0 92-93 33.562365412277174 37.0 33.0 37.0 27.0 37.0 94-95 33.424543947358984 37.0 33.0 37.0 27.0 37.0 96-97 33.315063571266776 37.0 33.0 37.0 27.0 37.0 98-99 33.15670000092045 37.0 33.0 37.0 27.0 37.0 100-101 31.458954505994548 33.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11027053714520463 2306 2 0.10597089528945247 2306 3 0.08344791968414711 2306 4 0.007132159523692394 2306 5 0.05777868050157764 2306 6 0.09659694984390654 2306 7 0.08343871653059409 2306 8 0.1172149285887798 2306 9 0.0917647538260482 2306 10-11 0.13213150033427468 2306 12-13 0.12319366212738458 2306 14-15 0.1477351524468773 2306 16-17 0.18058273529026536 2306 18-19 0.19021611090992963 2306 20-21 0.15346442345653344 2306 22-23 0.1505492588887094 2306 24-25 0.17067445776066137 2306 26-27 0.2009031868788682 2306 28-29 0.1853290603637845 2306 30-31 0.15943372001007106 2306 32-33 0.1411190538547018 2306 34-35 0.14563994330003283 2306 36-37 0.10616299316334477 2306 38-39 0.09339545503785018 2306 40-41 0.10772239084417379 2306 42-43 0.0964262147531798 2306 44-45 0.08907654832277245 2306 46-47 0.08672638495752949 2306 48-49 0.07407997845034942 2306 50-51 -0.015020869207894805 2306 52-53 -0.05421897843305601 2306 54-55 -0.060667932289611315 2306 56-57 -0.017277250803132915 2306 58-59 -0.11181677520853128 2306 60-61 -0.09310448100628577 2306 62-63 -0.06893877185547836 2306 64-65 -0.10879147854191018 2306 66-67 -0.2373779852027056 2306 68-69 -0.2981993869437858 2306 70-71 -0.262871761745437 2306 72-73 -0.21892511288195493 2306 74-75 -0.31556961581748766 2306 76-77 -0.47947679157495315 2306 78-79 -0.10799339879361725 2306 80-81 -0.0640937861108597 2306 82-83 -0.11721592412904158 2306 84-85 -0.10335752805686127 2306 86-87 -0.24097177814689275 2306 88-89 -0.08616578289935717 2306 90-91 -0.14436370028108314 2306 92-93 -0.2262457435366514 2306 94-95 -0.25256455768666797 2306 96-97 -0.3726412262045926 2306 98-99 -0.2240948729046508 2306 100-101 -0.40714875130293393 2307 1 0.10379191292634005 2307 2 0.09865271585778146 2307 3 0.07939926250671192 2307 4 0.03610775354296436 2307 5 0.033717657194252126 2307 6 0.0774924287872949 2307 7 0.09438232667015711 2307 8 0.11277914541299339 2307 9 0.10321798979003916 2307 10-11 0.12774539975048782 2307 12-13 0.12434328317318233 2307 14-15 0.156407455101089 2307 16-17 0.16939298772926747 2307 18-19 0.17767366233799464 2307 20-21 0.18374925807051312 2307 22-23 0.1849672774564013 2307 24-25 0.12672696960560614 2307 26-27 0.19447586894359148 2307 28-29 0.12730698692859477 2307 30-31 0.18895489810378763 2307 32-33 0.1325983589573667 2307 34-35 0.19759405611673486 2307 36-37 0.2068084401958572 2307 38-39 0.11280583455145887 2307 40-41 0.08998708030485858 2307 42-43 0.1659309876360595 2307 44-45 0.09949695051710705 2307 46-47 0.11939879285540655 2307 48-49 -0.005427552337863517 2307 50-51 0.0715873137473011 2307 52-53 -7.505539602519207E-4 2307 54-55 0.01702309058038054 2307 56-57 -0.015817006891467145 2307 58-59 0.032755371986105786 2307 60-61 -3.0991461311913326E-4 2307 62-63 0.012924593194362899 2307 64-65 -0.11336027405786808 2307 66-67 -0.16739018185237597 2307 68-69 -0.04954135618300626 2307 70-71 -0.16510886504172362 2307 72-73 -0.23676944779240472 2307 74-75 -0.3129279051718896 2307 76-77 -0.5171523265605877 2307 78-79 -0.20599105522592964 2307 80-81 -0.044314484907417295 2307 82-83 -0.13281790541201843 2307 84-85 -0.08147714624519864 2307 86-87 -0.129193495721033 2307 88-89 -0.09649260357020495 2307 90-91 -0.23485146315763927 2307 92-93 -0.1880794625856268 2307 94-95 -0.14293202516513048 2307 96-97 -0.46108063696810575 2307 98-99 -0.24161422061204263 2307 100-101 -0.24895914134091157 2201 1 0.09774870533275504 2201 2 0.0996056203241551 2201 3 0.0637727279835758 2201 4 0.04561959792246739 2201 5 0.02709517716396448 2201 6 0.059479131376178884 2201 7 0.09290007201155959 2201 8 0.09145199197392628 2201 9 0.0685965862490221 2201 10-11 0.11424062601761165 2201 12-13 0.0789748630040279 2201 14-15 0.17272255689614724 2201 16-17 0.18505996145043468 2201 18-19 0.20546206221779073 2201 20-21 0.14953826333926656 2201 22-23 0.13826553005794295 2201 24-25 0.18076099761024267 2201 26-27 0.14897534012749958 2201 28-29 0.1498727134329627 2201 30-31 0.1300793824903934 2201 32-33 0.08825990663196848 2201 34-35 0.1561979533000155 2201 36-37 0.14516560813090962 2201 38-39 0.14585405518860028 2201 40-41 0.11096693624320864 2201 42-43 0.1111297157742328 2201 44-45 0.09689839959199986 2201 46-47 0.12063526221039922 2201 48-49 0.11791996959495066 2201 50-51 0.10791347473651314 2201 52-53 0.04268414223945882 2201 54-55 0.006548488086252746 2201 56-57 0.03868008808080958 2201 58-59 0.027824318638742795 2201 60-61 0.03520061718194967 2201 62-63 0.021705087592444272 2201 64-65 0.008829226204113638 2201 66-67 -0.06666539370309721 2201 68-69 -0.05066802930032566 2201 70-71 -0.07789632806193225 2201 72-73 -0.1264387369029052 2201 74-75 -0.19653467179422535 2201 76-77 -0.10440388573147175 2201 78-79 -0.0017871444393833258 2201 80-81 0.014694621760177995 2201 82-83 0.03357424341032811 2201 84-85 0.047938915005254046 2201 86-87 0.05055727891851092 2201 88-89 -0.01130319279766212 2201 90-91 -0.026032110069266423 2201 92-93 -0.02681461167164656 2201 94-95 -0.047458381330706345 2201 96-97 -0.16463462117196315 2201 98-99 -0.020343823750906154 2201 100-101 -0.04328494415436879 1101 1 0.06132099709954275 1101 2 0.06844765353782378 1101 3 0.029432868732030215 1101 4 -0.023931358481100062 1101 5 0.004020435926747723 1101 6 0.019255933361776556 1101 7 0.06569017956999801 1101 8 0.08223365204452193 1101 9 0.07084453243539457 1101 10-11 0.06883506300765418 1101 12-13 0.07216992543823864 1101 14-15 0.07150282450744783 1101 16-17 0.07531366361010328 1101 18-19 0.08948699867616483 1101 20-21 0.03713358585331861 1101 22-23 0.057436792337547615 1101 24-25 0.08218876564891531 1101 26-27 0.11476166032029056 1101 28-29 0.09928293750588324 1101 30-31 0.008309845054057519 1101 32-33 0.045570371700740964 1101 34-35 0.06929025583985293 1101 36-37 0.03780921717145702 1101 38-39 0.04417993904612416 1101 40-41 0.001945672108959684 1101 42-43 0.0988350568185723 1101 44-45 0.1378674657881831 1101 46-47 0.1445296843364332 1101 48-49 0.061473293622825054 1101 50-51 0.09729717878306587 1101 52-53 -0.007643951194687304 1101 54-55 0.004887784494485459 1101 56-57 0.09127117650257333 1101 58-59 0.08372255836912501 1101 60-61 0.09309334889653087 1101 62-63 0.014387371951421812 1101 64-65 -0.0026606427506976615 1101 66-67 -0.03065922661765086 1101 68-69 -0.06515166558528307 1101 70-71 -0.13203995618646758 1101 72-73 -0.19521868331184322 1101 74-75 -0.19354355539156387 1101 76-77 -0.360956618962021 1101 78-79 0.03797022278711637 1101 80-81 0.13339736872582364 1101 82-83 0.05658286601394735 1101 84-85 0.033029447034643056 1101 86-87 0.04568182681610011 1101 88-89 0.0782812526484733 1101 90-91 0.02868945048503946 1101 92-93 -0.004037405965590324 1101 94-95 -0.05512521639342083 1101 96-97 -0.07548324454431565 1101 98-99 -0.08032415917756452 1101 100-101 -0.010983863167290053 2304 1 0.06565421685209571 2304 2 0.06833741482710565 2304 3 0.05315041262450393 2304 4 -0.046169673526065935 2304 5 -0.019134295933703527 2304 6 0.015281841523744788 2304 7 0.051035695703113504 2304 8 0.07542509929194807 2304 9 0.010727440066354177 2304 10-11 0.052384815173319055 2304 12-13 0.029242098232032276 2304 14-15 0.07345523890190009 2304 16-17 0.06690428605006105 2304 18-19 0.07623032723141421 2304 20-21 0.03128412978718842 2304 22-23 0.06845259018417948 2304 24-25 0.009714899015314415 2304 26-27 0.025914048681393353 2304 28-29 0.04893039256650411 2304 30-31 0.07742302326469996 2304 32-33 0.026133751968409058 2304 34-35 0.03718777797417516 2304 36-37 0.06369420438565498 2304 38-39 0.09191016603784874 2304 40-41 -0.0076228539255822625 2304 42-43 -0.04813745132381797 2304 44-45 -0.014409586806358732 2304 46-47 -0.006858430263662285 2304 48-49 -0.01059171203764464 2304 50-51 -0.016625634514802812 2304 52-53 -0.10171107097040277 2304 54-55 -0.09771614291943109 2304 56-57 -0.059208096928038856 2304 58-59 -0.07518599579656637 2304 60-61 0.012874883188509045 2304 62-63 -0.09440597019769115 2304 64-65 -0.08804841083650672 2304 66-67 -0.21927863093950606 2304 68-69 -0.11099988566424202 2304 70-71 -0.18855316821260004 2304 72-73 -0.14892025341361403 2304 74-75 -0.18879770550210395 2304 76-77 -0.4221269002774761 2304 78-79 -0.16065913511257435 2304 80-81 -0.00124509760397018 2304 82-83 -0.006853342021202025 2304 84-85 -0.035525173447631175 2304 86-87 -0.05512806571869788 2304 88-89 0.07621541503529272 2304 90-91 -0.011597884686814552 2304 92-93 -0.12698347293897427 2304 94-95 -0.1803728740882704 2304 96-97 -0.1547323883097249 2304 98-99 -0.1386368794559587 2304 100-101 0.008922906877796066 2202 1 0.045871744148549 2202 2 0.058760203154776036 2202 3 0.034822657346907704 2202 4 -0.012618002937159645 2202 5 0.011328500409668152 2202 6 0.05952291162685697 2202 7 0.018623785014682426 2202 8 0.06835848343028061 2202 9 -0.00275796596208977 2202 10-11 0.013531921193283836 2202 12-13 0.04855695387350778 2202 14-15 0.038205702414437326 2202 16-17 0.008620915185119316 2202 18-19 -0.028514947475734687 2202 20-21 0.0338075615593354 2202 22-23 0.08620665662940752 2202 24-25 -0.009134896997380793 2202 26-27 0.02375380688035733 2202 28-29 -6.377134951875973E-4 2202 30-31 0.054572069765157494 2202 32-33 0.010506680691413806 2202 34-35 0.03065628278420718 2202 36-37 0.07373442342547776 2202 38-39 0.08720038999736346 2202 40-41 0.04339046027305926 2202 42-43 0.09541850392739804 2202 44-45 0.011165681836651231 2202 46-47 0.08833538219209913 2202 48-49 0.049227410435868535 2202 50-51 0.003324015679986303 2202 52-53 0.038550772425679725 2202 54-55 0.03487037817748018 2202 56-57 0.017215007637304325 2202 58-59 0.009451538580101726 2202 60-61 0.06471334993819511 2202 62-63 0.04526743678773215 2202 64-65 -0.010275966003987946 2202 66-67 -0.12966487673145366 2202 68-69 -0.03625520361571688 2202 70-71 -0.041418060670785906 2202 72-73 -0.12641974003255285 2202 74-75 -0.15017914818748324 2202 76-77 -0.29625301584939834 2202 78-79 0.026098524667077072 2202 80-81 0.08487846625227746 2202 82-83 0.028562229579890186 2202 84-85 -0.003791398774602328 2202 86-87 0.010114982408374829 2202 88-89 0.03311573229019871 2202 90-91 -0.040306385122057975 2202 92-93 0.04374946482477782 2202 94-95 -0.04269143208946247 2202 96-97 -0.1793455482518027 2202 98-99 -0.01949470768062156 2202 100-101 0.09472617158863983 1102 1 0.05515397366632868 1102 2 0.06141354886825923 1102 3 0.02452807154769232 1102 4 -0.0382010604612546 1102 5 -0.06574807680109984 1102 6 -0.004396731177827462 1102 7 0.016231393603966637 1102 8 0.035546389926985 1102 9 0.0038448577494349934 1102 10-11 0.01998205786663476 1102 12-13 0.03557293590973387 1102 14-15 0.01563613254162277 1102 16-17 -0.0032433626703038954 1102 18-19 0.0052814696449488 1102 20-21 -0.008901739566780975 1102 22-23 0.027756772023245446 1102 24-25 0.004632070899909024 1102 26-27 0.02785099829433335 1102 28-29 0.021540287792852553 1102 30-31 -0.05932342855608397 1102 32-33 -0.007194219338003904 1102 34-35 -0.01589093530326835 1102 36-37 -0.08836690897469168 1102 38-39 -0.048937856363345134 1102 40-41 -0.05645390121579652 1102 42-43 -0.07436605162639154 1102 44-45 -0.08185393903450944 1102 46-47 -0.05972812032044317 1102 48-49 -0.07933826136183342 1102 50-51 -0.05847925065121018 1102 52-53 -0.1278226825961113 1102 54-55 -0.17913677031535258 1102 56-57 -0.13269388277626604 1102 58-59 -0.1441493723596423 1102 60-61 -0.11454076611395436 1102 62-63 -0.11360852532468613 1102 64-65 -0.15535286901602774 1102 66-67 -0.20652849535777307 1102 68-69 -0.18574998855391556 1102 70-71 -0.27855015278842643 1102 72-73 -0.2231179452658978 1102 74-75 -0.1924826560637385 1102 76-77 -0.5036234034384535 1102 78-79 -0.1899946654229865 1102 80-81 -0.06892207786727056 1102 82-83 -0.0782148708042456 1102 84-85 -0.08265230120191092 1102 86-87 -0.13985804294308224 1102 88-89 -0.20288337870251638 1102 90-91 -0.2672447114573515 1102 92-93 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1213 24-25 -0.33099302771918104 1213 26-27 -0.3639683490242973 1213 28-29 -0.3441786151773556 1213 30-31 -0.3158765848435934 1213 32-33 -0.311228959953219 1213 34-35 -0.2574971335681653 1213 36-37 -0.2836120957407928 1213 38-39 -0.19616659511947887 1213 40-41 -0.25575392158901167 1213 42-43 -0.17343118918300604 1213 44-45 -0.18057116401221407 1213 46-47 -0.35209560128699025 1213 48-49 -0.20044272771923488 1213 50-51 -0.11099381696420352 1213 52-53 -0.0522830436250743 1213 54-55 -0.0074066014798361834 1213 56-57 -0.041587433851333344 1213 58-59 -0.040539833853763696 1213 60-61 0.035717430398896965 1213 62-63 0.019485732008270418 1213 64-65 0.1045705506721859 1213 66-67 0.13636576711134296 1213 68-69 0.17313795624641415 1213 70-71 0.1329981611046307 1213 72-73 0.1562729859864831 1213 74-75 0.2295221575527009 1213 76-77 0.5583979465683626 1213 78-79 0.2404855091506235 1213 80-81 0.04140506734444216 1213 82-83 0.05479770747504631 1213 84-85 0.05926272242159314 1213 86-87 0.11195701846055783 1213 88-89 0.1617733094219389 1213 90-91 0.14182866995942334 1213 92-93 0.2755035171890796 1213 94-95 0.31223632280119773 1213 96-97 0.23985138193664568 1213 98-99 0.3176529252127338 1213 100-101 0.598858390963656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 35.0 8 435.0 9 1414.0 10 1036.0 11 401.0 12 359.0 13 678.0 14 1675.0 15 3992.0 16 7623.0 17 12198.0 18 16857.0 19 21926.0 20 27462.0 21 33460.0 22 40344.0 23 48575.0 24 59652.0 25 71812.0 26 88074.0 27 109377.0 28 137759.0 29 173343.0 30 222182.0 31 290071.0 32 387103.0 33 529846.0 34 788692.0 35 1429399.0 36 3337134.0 37 6018651.0 38 1674262.0 39 43.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.132801029432265 25.880689836670314 32.40264265122123 22.58386648267619 2 23.780797356002765 25.65590947556143 31.283299146858262 19.279994021577547 3 21.077949218296162 23.812517495800524 35.25901444470027 19.850518841203048 4 20.961399983221394 24.136670715585655 34.69496692737991 20.206962373813045 5 24.907589667808132 23.28410811341057 32.41814379122999 19.39015842755131 6 25.753142517725593 23.54076575429855 31.754975308432982 18.951116419542878 7 24.940507036908325 26.367134485089377 29.652653526796147 19.039704951206147 8 24.291879097090856 24.635960223923075 31.503827497022858 19.56833318196321 9 21.94160855223373 23.928848276353477 34.13995906634394 19.989584105068843 10-11 24.516593891494242 24.753898219377543 30.829640827698856 19.89986706142936 12-13 24.14632240445354 26.849160887085123 28.82509129999856 20.179425408462777 14-15 24.208454190043472 25.312022322653423 29.59475662241132 20.884766864891784 16-17 23.91018823469891 25.350158996557067 29.649393329797856 21.090259438946166 18-19 23.074219655917585 25.71845505153847 30.13198616286142 21.075339129682526 20-21 22.295251828606386 26.352147183503305 30.240114659954397 21.112486327935915 22-23 22.282629020284734 26.163631250542696 30.213568328418795 21.34017140075378 24-25 22.432099236663333 26.41929120034532 29.776933652448584 21.37167591054277 26-27 22.34431529458503 26.29287408475521 29.86316956416541 21.499641056494355 28-29 22.58432114942252 26.2539094203344 29.48594256479086 21.675826865452215 30-31 22.42082005934205 26.072686593828532 29.52093715293006 21.98555619389936 32-33 22.309569897947785 26.423034151094587 29.187295002642593 22.08010094831503 34-35 22.23991821238473 26.50187725504661 29.04408793636215 22.21411659620651 36-37 22.262582323798867 26.709566416862433 29.329857283154926 21.697993976183767 38-39 22.49045501989784 26.854558227894998 28.97037489102061 21.68461186118655 40-41 22.202964305187926 27.198214787872327 28.98916888612819 21.609652020811552 42-43 22.249677943827713 27.23114275703256 28.853043613069403 21.666135686070326 44-45 22.078333071355846 26.887776310618015 29.468183767359875 21.565706850666267 46-47 22.09367034513713 27.054708648281107 29.280322583417156 21.57129842316461 48-49 22.031919394188378 26.769132881507467 29.35132462154877 21.847623102755385 50-51 22.026449809090035 26.964085007546963 29.24963953246731 21.759825650895696 52-53 22.2176673615163 27.48167642308173 28.73533825747575 21.56531795792622 54-55 22.095259475051424 27.113919117931317 29.05914950835864 21.731671898658618 56-57 22.173242840724868 27.365006132580525 28.87003944539145 21.59171158130316 58-59 22.18747785458415 27.207335118736133 28.894081315342962 21.711105711336756 60-61 22.10364594087974 27.182869033281765 28.71281647110287 22.000668554735626 62-63 22.008399008890816 27.425628753353564 28.582461350594336 21.983510887161287 64-65 22.384997051617027 27.49703705293922 28.29435087505065 21.823615020393103 66-67 22.604130274558322 27.536877175519837 27.929170021419562 21.92982252850228 68-69 22.427663772790492 27.802303867879623 27.807435363914173 21.962596995415716 70-71 22.603211535910685 27.928567674141767 27.86598250428422 21.60223828566332 72-73 22.79151359954512 27.755552616998735 27.652423435966366 21.800510347489784 74-75 22.719138179883764 27.81281562995599 27.71020144342883 21.75784474673142 76-77 22.755263758343286 27.602556307367742 27.923317989771988 21.71886194451698 78-79 22.839410149449197 27.517721223970565 27.868117548980536 21.7747510775997 80-81 22.8527204447863 27.57654074254171 27.766465563738674 21.804273248933317 82-83 22.613808344091428 27.61890567157016 27.863975186784 21.903310797554415 84-85 22.23187921268102 28.003514758247384 27.757654219160056 22.00695180991154 86-87 22.172003404044943 27.75794588289519 27.800148830406723 22.269901882653144 88-89 21.887280891110866 27.99847391975796 27.806571228391917 22.30767396073926 90-91 21.845416512704652 28.099878405962837 27.8614814535285 22.193223627804013 92-93 21.912884328706948 28.455224952881853 27.528312340439697 22.103578377971502 94-95 21.962891787598434 28.555648894080132 27.431688014876304 22.04977130344513 96-97 21.870277204989353 28.727097110113824 27.339510842296335 22.063114842600488 98-99 22.140968652710065 28.56878358601989 27.23739791734925 22.052849843920797 100-101 22.44215904382468 28.701648754021196 26.82917284392768 22.027019358226443 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 154673.0 1 84200.5 2 19576.0 3 22511.5 4 21967.5 5 16573.0 6 7480.5 7 6861.0 8 8458.5 9 9361.5 10 8223.5 11 5739.0 12 3478.5 13 2105.5 14 1486.5 15 1278.5 16 1179.5 17 1111.5 18 1120.0 19 1174.0 20 1294.5 21 1419.0 22 1595.5 23 1854.5 24 2188.5 25 2612.0 26 3064.0 27 3698.0 28 4743.0 29 6432.0 30 9011.5 31 13990.0 32 44014.0 33 90470.5 34 145675.0 35 268953.5 36 381396.5 37 540148.5 38 731359.0 39 746684.5 40 709383.5 41 734201.0 42 830000.5 43 996585.5 44 1087518.0 45 1067530.0 46 975219.0 47 798646.0 48 660539.0 49 626921.0 50 577410.0 51 506164.0 52 463073.0 53 410440.0 54 356929.5 55 320525.0 56 278402.5 57 214880.0 58 154716.0 59 112382.0 60 81790.0 61 65262.5 62 55068.5 63 39867.5 64 26247.0 65 17959.5 66 13120.5 67 9930.5 68 8058.5 69 6483.5 70 4886.5 71 3823.5 72 3596.5 73 3337.0 74 2636.5 75 1940.0 76 1482.0 77 1366.0 78 1393.5 79 1224.0 80 834.0 81 507.5 82 345.5 83 263.0 84 196.5 85 136.0 86 116.0 87 116.0 88 95.0 89 78.5 90 77.5 91 72.5 92 69.0 93 57.0 94 38.0 95 34.0 96 33.5 97 18.0 98 9.5 99 5.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13710206527847715 2 0.0 3 0.0 4 0.18040829509977266 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.981528682878482E-4 12-13 0.0 14-15 5.374658427583493E-4 16-17 0.0 18-19 0.0 20-21 0.0034951371570992058 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.8275375098055326E-5 32-33 0.0 34-35 1.44826125294166E-4 36-37 2.478135921700174E-4 38-39 0.008988874843257904 40-41 0.0073024550731658365 42-43 9.622891436212364E-4 44-45 0.0011907925857520315 46-47 0.001882739628824158 48-49 1.1907925857520316E-4 50-51 9.301055602225328E-4 52-53 0.019400264072738503 54-55 0.017697752510947087 56-57 0.020970822942595238 58-59 0.04129475585887653 60-61 0.015213179872567172 62-63 0.002236759046209897 64-65 0.006980619239178801 66-67 0.012487230358696979 68-69 0.027922476956715205 70-71 0.011840340332383039 72-73 0.01882739628824158 74-75 0.0125934361839127 76-77 0.0233202245307006 78-79 0.00940726142744105 80-81 0.006694185346930339 82-83 0.0017958439536476585 84-85 0.019834742448621 86-87 0.009082207235114144 88-89 0.0125934361839127 90-91 0.046016087543466345 92-93 0.03537941323019482 94-95 0.029557402993369345 96-97 0.026725247654283434 98-99 0.03304932179212868 100-101 0.24352995721964996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5535871E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.796576541697977 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97958504050513 9.722785755772744 2 9.759947452668861 2.497878292420545 3 3.719369534341558 1.4278559079918391 4 2.0213223501074027 1.0346402467437685 5 1.1309029462858058 0.7235843056689033 6 0.8472790185884057 0.6505362488124765 7 0.6548658979017402 0.5866029110933173 8 0.3761671048603206 0.38509209198512173 9 0.39023492431117546 0.4494303970372505 >10 3.5288512934808955 9.60608423594779 >50 0.6404865218560345 5.75803532242893 >100 0.746091210896665 19.333774976224184 >500 0.07555444999370181 6.659139626410976 >1k 0.12094952700239607 31.56722699157392 >5k 0.006551245211256565 5.709360095256842 >10k+ 0.0018414819886897011 3.8879725946313033 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98035 0.6310235196983806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66219 0.4262329418157501 No Hit CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC 19607 0.12620470393967612 No Hit TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18974 0.12213026228140025 No Hit TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC 17372 0.11181864216045563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.673546336732585E-4 0.0 0.0 2 1.737913503529992E-4 0.0 0.0 3 1.802280670327399E-4 0.0 0.0 4 1.9310150039222133E-4 0.0 0.0 5 1.9953821707196205E-4 0.0 0.0 6 2.1241165043144346E-4 0.0 0.0 7 2.4459523383014704E-4 0.0 0.0 8 3.025256839478134E-4 0.0 0.0 9 3.604561340654798E-4 0.0 0.0 10-11 4.89190467660294E-4 0.0 0.0 12-13 7.402224181701818E-4 0.0 0.0 14-15 9.976910853598101E-4 0.0 0.0 16-17 0.001251941394209568 0.0 0.0 18-19 0.0016703279783927146 0.0 0.0 20-21 0.0022110121794909343 0.0 0.0 22-23 0.002832155339085913 0.0 0.0 24-25 0.00352732074049791 0.0 0.0 26-27 0.004470299734079924 0.0 0.0 28-29 0.005580633361335196 0.0 0.0 30-31 0.006639473255152543 0.0 0.0 32-33 0.007997620474577834 0.0 0.0 34-35 0.009133700968552068 0.0 0.0 36-37 0.010527250129715933 0.0 0.0 38-39 0.01203022347443539 0.0 0.0 40-41 0.013809975636383694 0.0 0.0 42-43 0.016075699907652424 0.0 0.0 44-45 0.018537744037653247 0.0 0.0 46-47 0.02154369072709216 3.2183583398703557E-6 0.0 48-49 0.02505170131755085 6.4367166797407114E-6 0.0 50-51 0.029364301492977127 6.4367166797407114E-6 0.0 52-53 0.03397299063567147 6.4367166797407114E-6 0.0 54-55 0.039579370863725635 6.4367166797407114E-6 0.0 56-57 0.04658251861128353 6.4367166797407114E-6 0.0 58-59 0.05494703193660658 1.2873433359481423E-5 0.0 60-61 0.0652039399657734 1.2873433359481423E-5 0.0 62-63 0.07701853343143747 1.2873433359481423E-5 0.0 64-65 0.09136597491057952 1.2873433359481423E-5 0.0 66-67 0.10973636431455952 1.2873433359481423E-5 0.0 68-69 0.13267038584447566 1.2873433359481423E-5 0.0 70-71 0.16016160278364824 1.2873433359481423E-5 0.0 72-73 0.1945948186619212 1.2873433359481423E-5 0.0 74-75 0.2364109485718567 1.2873433359481423E-5 0.0 76-77 0.28803985306005697 1.2873433359481423E-5 0.0 78-79 0.35146082250554217 1.2873433359481423E-5 0.0 80-81 0.42915199283001254 1.2873433359481423E-5 6.4367166797407114E-6 82-83 0.52330184770458 1.2873433359481423E-5 6.4367166797407114E-6 84-85 0.6379011514706836 1.2873433359481423E-5 6.4367166797407114E-6 86-87 0.7769889438448607 1.9310150039222132E-5 6.4367166797407114E-6 88-89 0.9441665678094263 1.9310150039222132E-5 6.4367166797407114E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTGT 35360 0.0 57.130444 1 CTTTGTG 96365 0.0 47.527126 1 TTGTGTT 107365 0.0 44.11012 3 TTTGTGT 105120 0.0 43.68104 2 TGTGTTT 116310 0.0 40.987682 4 GTGTTTG 121255 0.0 39.290234 5 CCGATCT 660 0.0 30.214508 8 TTGAGGG 38040 0.0 29.656338 9 TTGAGTC 8435 0.0 28.929993 4 TTTGAGG 52835 0.0 28.532 8 GTTTGAG 99315 0.0 28.354582 7 TGTTTGA 175620 0.0 27.16268 6 GTTTGAC 24665 0.0 26.738148 7 TTTGACG 9890 0.0 25.15621 8 TTGACGG 7935 0.0 24.2337 9 AACTTTG 26195 0.0 23.988354 2 TTTGATT 26520 0.0 23.837872 3 TAACTTT 26325 0.0 23.7398 1 CTTCCGA 885 0.0 23.605846 5 GTTTGAT 48615 0.0 23.381052 7 >>END_MODULE