FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30292.34100000035c02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30292.34100000035c02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535871
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980350.6310235196983806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662190.4262329418157501No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC196070.12620470393967612No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189740.12213026228140025No Hit
TTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCC173720.11181864216045563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT353600.057.1304441
CTTTGTG963650.047.5271261
TTGTGTT1073650.044.110123
TTTGTGT1051200.043.681042
TGTGTTT1163100.040.9876824
GTGTTTG1212550.039.2902345
CCGATCT6600.030.2145088
TTGAGGG380400.029.6563389
TTGAGTC84350.028.9299934
TTTGAGG528350.028.5328
GTTTGAG993150.028.3545827
TGTTTGA1756200.027.162686
GTTTGAC246650.026.7381487
TTTGACG98900.025.156218
TTGACGG79350.024.23379
AACTTTG261950.023.9883542
TTTGATT265200.023.8378723
TAACTTT263250.023.73981
CTTCCGA8850.023.6058465
GTTTGAT486150.023.3810527
Produced by FastQC (version 0.11.2)