FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30291.34100000035bb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30291.34100000035bb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12310450
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219210.17806822658798013No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT187430.1522527608657685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149590.12151464812415469No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC139320.11317214236685094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT440050.058.9030381
CTTTGTG1113250.050.9351841
TTTGTGT1231150.046.1624642
TTGTGTT1279050.045.694413
TGTGTTT1362150.043.042944
GTGTTTG1518650.038.621445
AACTTTG240650.032.9894562
TAACTTT243300.032.57561
TTGAGGG369450.031.9491429
TTTGAGG546350.031.7810138
GTTTGAG1180650.030.837187
TGTTTGA1979100.029.6455176
GTTTGAC335950.027.2769877
TTGACGG80250.025.8553339
TTTGACG118550.025.8328258
TTTGACC101200.025.0069338
TTGAGTC154150.024.9328844
TTGACCC70100.024.3158879
TTGAGGC77100.023.7093729
GTTTGAT484600.022.407667
Produced by FastQC (version 0.11.2)